Article
Plant Sciences
Maolong Hu, Hongkun Zhang, Lingna Kong, Juanjuan Ma, Ting Wang, Xinyu Lu, Yue Guo, Jiefu Zhang, Rongzhan Guan, Pu Chu
Summary: The application of herbicides is an effective strategy for weed control, and the development of herbicide-resistant crops can enhance weed management. However, the use of the herbicide tribenuron-methyl (TBM) is limited in rapeseed fields due to rapeseed's sensitivity to TBM. A study was conducted on the TBM-resistant rapeseed mutant M342 and its wild-type plants, revealing that M342 exhibited improved tolerance to TBM and had higher levels of proteins related to non-target-site resistance (NTSR) to herbicides compared to the wild-type plants. Differentially accumulated proteins (DAPs) between the two genotypes were enriched in glutathione metabolism and oxidoreduction coenzyme metabolic process, providing protection against oxidative stress caused by TBM. These findings offer insights into the mechanism of NTSR in plants and contribute to the development of herbicide-resistant crops.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Caiyue Liu, Tianbao Zhang, Xinsen Yang, Liu Wang, Yan Long, Agula Hasi, Xinwu Pei
Summary: In this study, a mutant with resistance to ALS-inhibiting herbicides was obtained from the elite cultivar Longya10 through EMS mutagenesis. The mutant showed significantly increased resistance to TBM and slight resistance to imazethapyr compared to Longya10. Biochemical analysis revealed a Pro197Ser mutation in the LuALS1 gene, which conferred high resistance to sulfonylurea herbicides in the mutant. Two CAPS markers were developed based on this mutation site for marker assistant selection in breeding. The mutant did not cause yield losses in natural field conditions, making it a valuable germplasm resource for breeding herbicide-resistant flax varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Xiangyang Qin, Cheng Yang, Mengmeng Hu, Yunxia Duan, Na Zhang, Jinxin Wang, Hengzhi Wang, Weitang Liu
Summary: Black-grass population HN-14 in Chinese wheat fields has developed resistance to mesosulfuron-methyl through target-site mutation. It also exhibits varying degrees of resistance to fenoxaprop-P-ethyl, clodinafop-propargyl, haloxyfop-methyl, and fluazifop-P-butyl, while remaining sensitive to other herbicides.
Article
Plant Sciences
Shmuel Galili, Joseph Hershenhorn, Marvin Edelman, Vladimir Sobolev, Evgeny Smirnov, Orit Amir-Segev, Aharon Bellalou, Evgenia Dor
Summary: Chickpea is an important crop in Israel, but sensitive to imidazolinone herbicides. A chickpea line resistant to these herbicides was developed using chemical mutagenesis, with a point mutation detected in one of the genes encoding the AHAS catalytic subunit. The mutation provided resistance to a specific group of herbicides and showed single-gene semi-dominant inheritance pattern, as confirmed by genetic transformation in tobacco plants.
Article
Plant Sciences
Ryan Widianto, Denny Kurniadie, Dedi Widayat, Uum Umiyati, Ceppy Nasahi, Santika Sari, Abdul Shukor Juraimi, Hisashi Kato-Noguchi
Summary: Monochoria vaginalis is an aquatic herbaceous plant that is often found as a noxious weed in paddy fields. This research evaluated the resistance levels of M. vaginalis to different groups of herbicides and investigated the mutations in the ALS gene of the resistant biotype. The study found that the resistant biotype had varying levels of resistance to different herbicides and had specific amino acid substitutions in the ALS protein. Further research is needed to understand the molecular basis of resistance mechanisms and to develop control strategies.
Article
Agronomy
Yi Cao, Xinxin Zhou, Zhaofeng Huang
Summary: The study revealed the molecular basis for resistance of Amaranthus retroflexus to ALS-inhibiting herbicides, with Trp-574-Leu and Gly-654-Tyr substitutions in ALS contributing to broad-spectrum resistance. The Gly-654-Tyr substitution, previously unreported, conferred high resistance to multiple classes of ALS-inhibiting herbicides in the R-Tyr654 population. Despite this resistance, effective control of the population was achieved with herbicides targeting other sites of action.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Wegi A. Wuddineh, Xia Xu, Gan-Yuan Zhong
Summary: This study identified VvALS1 as the best candidate gene for providing resistance to ALS-inhibiting herbicides in grapevine. The researchers found that chlorsulfuron and imazapyr herbicides showed a linear response curve in grapevine callus and in vitro shoots. By introducing amino acid substitutions in VvALS gene, the transgenic plants exhibited high resistance to chlorsulfuron or imazapyr herbicides. This research highlights the potential use of VvALS mutations as a selectable marker and enables gene-editing in grapevine improvement.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Agronomy
Axelle Koreki, Severine Michel, Caroline Lebeaux, Lidwine Trouilh, Christophe Delye
Summary: This study investigates the prevalence, mode of evolution, and spread of resistance-endowing ALS alleles in corn poppy populations in France. With the use of massively parallel amplicon sequencing, it was found that mutant ALS alleles have complex geographic distributions, which could be attributed to large population sizes, multiple independent mutation events, and human-mediated spread of resistance. The study also reveals that mutant ALS alleles are associated with resistance to the synthetic auxin 2,4-D.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Tiancheng Lou, Ke Wang, Junmin Chen, Jingjing Cao, Tao Gu, Liben Jiang, Yuanlai Lou, Rongxiang Cao, Hongchun Wang
Summary: Tribenuron-methyl-resistant cleavers have been reported in the major winter wheat farming region in China. The study evaluated the frequency and distribution of multiple herbicide resistance in cleavers populations across Jiangsu Province from 2017 to 2020. The results highlight the importance of diversity in herbicide sites of action for effective weed management.
Article
Agronomy
Lei Chen, Gang Gu, Chengxu Wang, Zhufeng Chen, Wei Yan, Man Jin, Gang Xie, Junli Zhou, Xing Wang Deng, Xiaoyan Tang
Summary: Herbicide resistance is valuable for integrated weed management in agriculture, particularly in rice fields where weedy rice poses a challenge. This study obtained three mutant variants of the OsALS gene in rice through ethyl methyl sulfonate mutagenesis, with W-548 M mutant showing high levels of resistance to a broad spectrum of herbicides. The stable inheritance of herbicide resistance in rice lines makes W-548 M mutation a valuable resource for breeding herbicide resistant rice and weed management.
Article
Agronomy
Barbara Wrzesinska, Tadeusz Praczyk
Summary: Centaurea cyanus, a weed species commonly found in fields with cereals, sugar beet, and maize, shows resistance mainly against acetolactate synthase (ALS) inhibitors. Genetic variability in ALS sequence has been observed among resistant plants, with four amino acid sequence changes identified in some resistant plants compared to susceptible ones. Further research is needed to confirm the significance of these mutations in herbicide resistance development.
Article
Biochemistry & Molecular Biology
Lucie Meyer, Fanny Pernin, Severine Michel, Geraldine Bailly, Bruno Chauvel, Valerie Le Corre, Christophe Delye
Summary: Assessing the capacity of weeds to develop herbicide resistance before it occurs in the field is crucial for effective chemical weed control. This study used herbicide selection and controlled crosses to accelerate the evolution of resistance to two commonly used herbicides in Ambrosia artemisiifolia. The results showed that non-target-site-based resistance (NTSR) to herbicides was significantly increased in just two generations of selection. NTSR to the tested herbicides emerged and was reinforced in subsequent generations. Further bioassays confirmed the emergence of resistance to imazamox and/or tribenuron in multiple A. artemisiifolia populations. NTSR was found to be the predominant type of resistance, with only one population showing a mutant ALS allele in addition to NTSR. This study highlights the importance of pre-emptive assessment of resistance evolution in weed populations.
Article
Agriculture, Multidisciplinary
Hudson K. Takano, Zoltan L. Benko, Moriah M. Zielinski, Adel Hamza, Christopher A. Kalnmals, Joshua J. Roth, Karla Bravo-Altamirano, Thomas Siddall, Norbert Satchivi, Jeffrey B. Church, Dilpreet S. Riar
Summary: Herbicides are effective tools for managing weeds and ensuring food production. Corteva Agriscience R&D has discovered new diphenyl-ether compounds with excellent postemergent efficacy on important weed species and corn safety. Through research in chemistry, biology, biochemistry, and computational modeling, the primary mode of action for these compounds was elucidated as targeting the acetolactate synthase (ALS) enzyme. These compounds have a unique biochemical profile and show excellent herbicidal activity and corn selectivity, making them a significant development in the search for novel, safe, and sustainable weed control solutions.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Bochui Zhao, Xian Xu, Binghua Li, Zhizun Qi, Jinan Huang, Ali Hu, Guiqi Wang, Xiaomin Liu
Summary: This study found that a population of D. sanguinalis from Hebei Province, China, displayed 6.9-fold resistance to nicosulfuron. Both ALS gene mutation and increased activity of GSTs enzyme were identified as the resistance mechanisms.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Plant Sciences
Sarah Shah, Thierry Lonhienne, Cody-Ellen Murray, Yibi Chen, Katherine E. Dougan, Yu Shang Low, Craig M. Williams, Gerhard Schenk, Gimme H. Walter, Luke W. Guddat, Cheong Xin Chan
Summary: This study identified 12 common herbicide-target genes and their proteins from seven species of Weeds of National Significance in Australia using high-throughput genome sequencing. The study found high sequence conservation at the herbicide-target sites among the diverse weed species. Mutations observed in the proteins of fireweed and parthenium suggest resistance of these weeds to certain herbicides. These findings provide important insights for herbicide discovery and development.
FRONTIERS IN PLANT SCIENCE
(2022)
