Article
Agronomy
Yi Cao, Xinxin Zhou, Zhaofeng Huang
Summary: The study revealed the molecular basis for resistance of Amaranthus retroflexus to ALS-inhibiting herbicides, with Trp-574-Leu and Gly-654-Tyr substitutions in ALS contributing to broad-spectrum resistance. The Gly-654-Tyr substitution, previously unreported, conferred high resistance to multiple classes of ALS-inhibiting herbicides in the R-Tyr654 population. Despite this resistance, effective control of the population was achieved with herbicides targeting other sites of action.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Hai-Lian Wang, Hao-Ran Li, Yi-Chi Zhang, Wen-Tao Yang, Zheng Yao, Ren-Jun Wu, Cong-Wei Niu, Yong-Hong Li, Jian-Guo Wang
Summary: A series of 42 novel sulfonylurea compounds were synthesized and evaluated for their herbicidal activities against monocotyledon weed species, showing potential for safe and effective use in peanut fields at ultralow dosages. The chemical structure and electronic properties were found to influence the compounds' behavior towards different plant species.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agronomy
Lei Chen, Gang Gu, Chengxu Wang, Zhufeng Chen, Wei Yan, Man Jin, Gang Xie, Junli Zhou, Xing Wang Deng, Xiaoyan Tang
Summary: Herbicide resistance is valuable for integrated weed management in agriculture, particularly in rice fields where weedy rice poses a challenge. This study obtained three mutant variants of the OsALS gene in rice through ethyl methyl sulfonate mutagenesis, with W-548 M mutant showing high levels of resistance to a broad spectrum of herbicides. The stable inheritance of herbicide resistance in rice lines makes W-548 M mutation a valuable resource for breeding herbicide resistant rice and weed management.
Article
Biology
Rui Zhang, Sha Chen, Xiangbing Meng, Zhuangzhuang Chai, Delin Wang, Yuge Yuan, Kunling Chen, Linjian Jiang, Jiayang Li, Caixia Gao
Summary: Utilizing genome editing, various missense mutations were created in rice to confer tolerance to different herbicides, with a novel triple mutant showing high tolerance to all tested herbicides. Field trials suggested that these mutated varieties have potential practical applications in weed control.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Plant Sciences
Candelario Palma-Bautista, Jose G. Vazquez-Garcia, Maria D. Osuna, Blanca Garcia-Garcia, Joel Torra, Joao Portugal, Rafael De Prado
Summary: A study in southern Spain found that Sinapis alba populations have developed resistance to the acetolactate synthase-inhibiting herbicide tribenuron-methyl. The resistant populations showed significantly higher resistance to tribenuron-methyl compared to the susceptible population. A mutation in the ALS gene and P450-mediated metabolism were identified as contributing factors to the resistance. Alternative chemical control methods were effective in controlling both the resistant and susceptible populations.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Axelle Koreki, Severine Michel, Caroline Lebeaux, Lidwine Trouilh, Christophe Delye
Summary: This study investigates the prevalence, mode of evolution, and spread of resistance-endowing ALS alleles in corn poppy populations in France. With the use of massively parallel amplicon sequencing, it was found that mutant ALS alleles have complex geographic distributions, which could be attributed to large population sizes, multiple independent mutation events, and human-mediated spread of resistance. The study also reveals that mutant ALS alleles are associated with resistance to the synthetic auxin 2,4-D.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Barbara Wrzesinska, Tadeusz Praczyk
Summary: Centaurea cyanus, a weed species commonly found in fields with cereals, sugar beet, and maize, shows resistance mainly against acetolactate synthase (ALS) inhibitors. Genetic variability in ALS sequence has been observed among resistant plants, with four amino acid sequence changes identified in some resistant plants compared to susceptible ones. Further research is needed to confirm the significance of these mutations in herbicide resistance development.
Article
Plant Sciences
Maolong Hu, Hongkun Zhang, Lingna Kong, Juanjuan Ma, Ting Wang, Xinyu Lu, Yue Guo, Jiefu Zhang, Rongzhan Guan, Pu Chu
Summary: The application of herbicides is an effective strategy for weed control, and the development of herbicide-resistant crops can enhance weed management. However, the use of the herbicide tribenuron-methyl (TBM) is limited in rapeseed fields due to rapeseed's sensitivity to TBM. A study was conducted on the TBM-resistant rapeseed mutant M342 and its wild-type plants, revealing that M342 exhibited improved tolerance to TBM and had higher levels of proteins related to non-target-site resistance (NTSR) to herbicides compared to the wild-type plants. Differentially accumulated proteins (DAPs) between the two genotypes were enriched in glutathione metabolism and oxidoreduction coenzyme metabolic process, providing protection against oxidative stress caused by TBM. These findings offer insights into the mechanism of NTSR in plants and contribute to the development of herbicide-resistant crops.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Sarah Shah, Thierry Lonhienne, Cody-Ellen Murray, Yibi Chen, Katherine E. Dougan, Yu Shang Low, Craig M. Williams, Gerhard Schenk, Gimme H. Walter, Luke W. Guddat, Cheong Xin Chan
Summary: This study identified 12 common herbicide-target genes and their proteins from seven species of Weeds of National Significance in Australia using high-throughput genome sequencing. The study found high sequence conservation at the herbicide-target sites among the diverse weed species. Mutations observed in the proteins of fireweed and parthenium suggest resistance of these weeds to certain herbicides. These findings provide important insights for herbicide discovery and development.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Caiyue Liu, Tianbao Zhang, Xinsen Yang, Liu Wang, Yan Long, Agula Hasi, Xinwu Pei
Summary: In this study, a mutant with resistance to ALS-inhibiting herbicides was obtained from the elite cultivar Longya10 through EMS mutagenesis. The mutant showed significantly increased resistance to TBM and slight resistance to imazethapyr compared to Longya10. Biochemical analysis revealed a Pro197Ser mutation in the LuALS1 gene, which conferred high resistance to sulfonylurea herbicides in the mutant. Two CAPS markers were developed based on this mutation site for marker assistant selection in breeding. The mutant did not cause yield losses in natural field conditions, making it a valuable germplasm resource for breeding herbicide-resistant flax varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Nicolas Loste, Graciela M. Nestares, Silvina A. Felitti, Ana C. Ochogavia
Summary: Chemically induced male sterility (CIMS) is a valuable tool for hybrid breeding in crops, and the gametocidal effect of the herbicide imazapyr on sunflowers has been investigated at the molecular level. The comparative transcriptomic analysis revealed genes related to xenobiotic metabolism and stress as potentially involved in the male sterility induction process. These findings offer new insights into the molecular mechanisms underlying male sterility induction in sunflowers.
Article
Agronomy
Xiangyang Qin, Cheng Yang, Mengmeng Hu, Yunxia Duan, Na Zhang, Jinxin Wang, Hengzhi Wang, Weitang Liu
Summary: Black-grass population HN-14 in Chinese wheat fields has developed resistance to mesosulfuron-methyl through target-site mutation. It also exhibits varying degrees of resistance to fenoxaprop-P-ethyl, clodinafop-propargyl, haloxyfop-methyl, and fluazifop-P-butyl, while remaining sensitive to other herbicides.
Article
Food Science & Technology
So Hee Yang, Euyeon Kim, Hyosun Park, Yeonjong Koo
Summary: Herbicide resistance is an important trait in improving farming methods and crop productivity. This study demonstrates the usefulness of using CRISPR-Cas9 system to edit herbicide binding protein genes in tomatoes and confirms the feasibility of targeting site editing through sgRNA efficiency testing. The success of breeding herbicide-resistant transgenic tomatoes highlights the value of CRISPR-Cas9 as a tool for crop improvement.
APPLIED BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Shmuel Galili, Joseph Hershenhorn, Marvin Edelman, Vladimir Sobolev, Evgeny Smirnov, Orit Amir-Segev, Aharon Bellalou, Evgenia Dor
Summary: Chickpea is an important crop in Israel, but sensitive to imidazolinone herbicides. A chickpea line resistant to these herbicides was developed using chemical mutagenesis, with a point mutation detected in one of the genes encoding the AHAS catalytic subunit. The mutation provided resistance to a specific group of herbicides and showed single-gene semi-dominant inheritance pattern, as confirmed by genetic transformation in tobacco plants.
Article
Agriculture, Multidisciplinary
Juliusz Pernak, Michal Niemczak, Tomasz Rzemieniecki, Katarzyna Marcinkowska, Tadeusz Praczyk
Summary: This study synthesized and characterized dicationic herbicidal ionic liquids containing different herbicidal anions with various modes of action. The HILs showed better performance in weed control compared to traditional herbicides, with the alkyl chain length in the cation influencing efficacy.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)