Review
Biotechnology & Applied Microbiology
Serik Eliby, Sara Bekkuzhina, Olena Kishchenko, Gulnur Iskakova, Gulnar Kylyshbayeva, Satyvaldy Jatayev, Kathleen Soole, Peter Langridge, Nikolai Borisjuk, Yuri Shavrukov
Summary: Doubled haploid production is a valuable biotechnology that accelerates the breeding of new wheat varieties and plays important roles in studying genetic control, marker-assisted selection, genomics, and genetic engineering in wheat. This paper reviews recent advances in androgenesis and gynogenesis techniques, with a focus on the in vitro culture phase, and the emerging innovative approaches in researching and producing wheat doubled haploids. The use of CRISPR-based genome editing for inducing doubled haploids in wheat is also discussed.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Plant Sciences
Monika Gajecka, Marek Marzec, Beata Chmielewska, Janusz Jelonek, Justyna Zbieszczyk, Iwona Szarejko
Summary: This study investigated the genotype-dependent molecular differences in chloroplast differentiation leading to the formation of green and albino regenerants in microspore culture of barley. The results showed that the failed transition from NEP-dependent to PEP-dependent transcription in plastids was associated with albino regenerants, while a high activity of PEP polymerase and increased expression of certain genes correlated with the production of green regenerants. Understanding these molecular mechanisms may help overcome the issue of albinism in cereal androgenesis.
Review
Plant Sciences
Brett Hale, Alison M. R. Ferrie, Sreekala Chellamma, J. Pon Samuel, Gregory C. Phillips
Summary: Androgenesis is widely used in plant breeding programs to redirect cell fate and study cell cycle regulation, meiotic recombination, and embryogenesis. The focus has shifted from protocol development to understanding how protocols work in order to extend their application to other genotypes and species. Transcriptomic and epigenetic analyses have revealed cellular and molecular responses associated with androgenesis, and gene transfer and genome editing technologies show promise for enhancing the genetic composition of microspores used in breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Antonio Calabuig-Serna, Carolina Camacho-Fernandez, Ricardo Mir, Rosa Porcel, Esther Carrera, Isabel Lopez-Diaz, Jose M. Segui-Simarro
Summary: During eggplant microspore embryogenesis, the transition from embryos to calli instead of developing as true embryos is a bottleneck. Studies show that there are no genotype-specific endogenous GR profiles associated with a high embryogenic response. The role of GRs in the development of microspore-derived embryos in eggplant cultures is highlighted, with different GR accumulation patterns influencing the embryogenic response.
PLANT GROWTH REGULATION
(2022)
Review
Biology
Ricardo Mir, Antonio Calabuig-Serna, Jose M. Segui-Simarro
Summary: This review compiles the latest advances in doubled haploid plant production, the application of doubled haploid lines in breeding programs, and future perspectives for alternative technologies for doubled haploid generation in eggplant. Eggplant is globally cultivated for its edible fruit, and breeding programs aim to produce F1 hybrids by crossing highly homozygous pure lines. Doubled haploid individuals can be induced from haploid cells in a single generation, offering a more efficient alternative to traditional self crossing methods.
Article
Plant Sciences
Carolina Camacho-Fernandez, Jose M. Segui-Simarro, Ricardo Mir, Kim Boutilier, Patricia Corral-Martinez
Summary: Different types of embryogenic structures exhibit specific cell wall characteristics that determine their developmental fate. Highly embryogenic structures have thin walls rich in specific components, while less embryogenic structures have thick walls and lower values of cell wall components.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Agronomy
Agnieszka Kielkowska, Waldemar Kiszczak
Summary: This paper presents the historical background and current status of haploid induction in carrot, and discusses the potential of haploidization technology in carrot breeding. The methods of androgenesis, gynogenesis, and induced parthenogenesis, as well as the manipulation of the centromere-specific histone 3 variant (CENH3) in carrot, are also acknowledged.
Review
Agronomy
Marzena Warchol, Edyta Skrzypek, Katarzyna Juzon-Sikora, Dragana Jakovljevic
Summary: Plant in vitro cultures are essential for improving crops and advancing plant biotechnology. They offer advantages over traditional breeding methods and can speed up the development of new cultivars. However, efficient methods for oat regeneration through in vitro culture are still lacking. This review provides an overview of the in vitro techniques used or potentially involved in oat breeding.
Article
Biotechnology & Applied Microbiology
Vineeta Shrivastava, Arockiasamy Savarimuthu, Manoj Patil, Purbasha Sarkar, Sainath Hadole, Santanu Dasgupta
Summary: Development of haploid and doubled haploids in Jatropha microspore culture was achieved through the establishment of an efficient isolated microspore culture system and optimization of various factors affecting microspore embryogenesis. Microscopic and flow cytometric analyses confirmed the success of gametic embryogenesis in Jatropha microspore culture.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Plant Sciences
Brett Hale, Callie Phipps, Naina Rao, Caroline Kelley, Gregory C. Phillips
Summary: The study successfully diverted isolated soybean microspores from a gametophytic pathway through temperature stress, as indicated by transcriptomic analysis. Cytological characteristics observed in isolated microspore culture supported previous claims of cell fate redirection in soybean microspores. This research provides insights into gametophytic embryogenesis induction and may be useful for soybean androgenesis investigation.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2021)
Article
Plant Sciences
Samira Tajedini, Baratali Fakheri, Mohsen Niazian, Nafiseh Mahdinezhad, Abdelbagi Mukhtar Ali Ghanim, Ali Kazemi Pour, Ivan Ingelbrecht, Mehran E. Shariatpanahi
Summary: Two different androgenesis pathways, shed-microspore culture (SMC) and anther culture (AC), were developed for haploid induction in mutagenized M-1 rice cultivars. The effects of various factors on induction and regeneration phases were evaluated. The SMC method showed better results in terms of the number of regenerated embryos. Chromosome counting and flow cytometry analysis confirmed the presence of haploid plants, providing a valuable foundation for rice breeding research.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Multidisciplinary Sciences
Byomkesh Dash, Sudhansu Sekhar Bhuyan, Sandeep Kumar Singh, Manjusha Chandravani, Nibedita Swain, Prachitara Rout, Jawahar Lal Katara, C. Parameswaran, B. N. Devanna, Sanghamitra Samantaray
Summary: Rice is crucial for global food security. This study developed an androgenic protocol for indica rice, contributing to yield improvement.
Article
Plant Sciences
S. K. Singh, K. P. Jeughale, B. Dash, S. S. Bhuyan, M. Chandravani, C. Parameswaran, B. N. Devanna, R. L. Verma, J. L. Katara, S. Samantaray
Summary: This study proposes a method for identifying haploids without the use of chemicals or complex laboratory facilities, utilizing morphological features and simple microscopic examinations. In addition to plant height, other morphological traits such as leaf number, tiller number, and floral characters can be used to successfully differentiate haploids.
BIOLOGIA PLANTARUM
(2023)
Article
Agronomy
Ade Mulyana, Bambang S. Purwoko, Iswari S. Dewi, Awang Maharijaya
Summary: Eggplant is a highly produced and valuable vegetable crop. Anther culture is used to produce pure lines for hybrid breeding. This study compared six anther culture methods and found that the Modification 1 method was the most effective in producing in vitro androgenic regenerated plants in eggplant. The ploidy level of regenerants was confirmed using flow cytometry.
Review
Food Science & Technology
Ewa Dubas, Iwona Zur, Jana Moravcikova, Jozsef Fodor, Monika Krzewska, Ewa Surowka, Anna Nowicka, Zuzana Gersi
Summary: This study reviews the signaling role of proteins and peptides in the stress-induced microspore embryogenesis processes, providing insights into microspore reprogramming and embryo development. It discusses the potential impact on DHs production and emphasizes the advancements in synthetic peptides for genome-editing techniques in plant science and breeding.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2021)
Article
Horticulture
Claudia M. Liberatore, Antonio Calabuig-Serna, Margherita Rodolfi, Benedetta Chiancone, Jose M. Segui-Simarro
SCIENTIA HORTICULTURAE
(2019)
Article
Plant Sciences
Patricia Corral-Martinez, Charlotte Siemons, Anneke Horstman, Gerco C. Angenent, Norbert de Ruijter, Kim Boutilier
PLANT REPRODUCTION
(2020)
Article
Agronomy
Alba Rivas-Sendra, Patricia Corral-Martinez, Carolina Camacho-Fernandez, Rosa Porcel, Jose M. Segui-Simarro
Article
Biotechnology & Applied Microbiology
Sara Sohrabi, Mohammad Reza Abdollahi, Asghar Mirzaie-Asl, Hassan Ebrahimi Koulaei, Mohsen Aghaeezadeh, Jose M. Segui-Simarro
Summary: The study found that pretreatments with 0.5 M mannitol and 50μM 5-AzaC, as well as the use of MS basal medium and 60 g/L sucrose, notably improved the efficiency of haploid induction and plant regeneration through ovule culture in sugar beet. This new method could potentially be applicable to other crops as well.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Biotechnology & Applied Microbiology
Esin Ari, Hilal Bedir, Ismail Gokhan Deniz, Ilker Genc, Jose M. Segui-Simarro
Summary: The study focused on exploring the androgenic potential of different wild Turkish V. hispanica genotypes as a basis for identifying bottlenecks in future studies. While almost all genotypes responded to microspore embryogenesis, three main bottlenecks were identified: foam-producing saponins in flowers hindered efficient isolation of microspores, embryos had reduced germination ability, and a dense network of hairy roots prevented the formation of functional root systems.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Review
Biology
Ricardo Mir, Antonio Calabuig-Serna, Jose M. Segui-Simarro
Summary: This review compiles the latest advances in doubled haploid plant production, the application of doubled haploid lines in breeding programs, and future perspectives for alternative technologies for doubled haploid generation in eggplant. Eggplant is globally cultivated for its edible fruit, and breeding programs aim to produce F1 hybrids by crossing highly homozygous pure lines. Doubled haploid individuals can be induced from haploid cells in a single generation, offering a more efficient alternative to traditional self crossing methods.
Article
Plant Sciences
Antonio Calabuig-Serna, Carolina Camacho-Fernandez, Ricardo Mir, Rosa Porcel, Esther Carrera, Isabel Lopez-Diaz, Jose M. Segui-Simarro
Summary: During eggplant microspore embryogenesis, the transition from embryos to calli instead of developing as true embryos is a bottleneck. Studies show that there are no genotype-specific endogenous GR profiles associated with a high embryogenic response. The role of GRs in the development of microspore-derived embryos in eggplant cultures is highlighted, with different GR accumulation patterns influencing the embryogenic response.
PLANT GROWTH REGULATION
(2022)
Article
Plant Sciences
Carolina Camacho-Fernandez, Jose M. Segui-Simarro, Ricardo Mir, Kim Boutilier, Patricia Corral-Martinez
Summary: Different types of embryogenic structures exhibit specific cell wall characteristics that determine their developmental fate. Highly embryogenic structures have thin walls rich in specific components, while less embryogenic structures have thick walls and lower values of cell wall components.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Hilal Bedir, Esin Ari, Gulsun Elif Vural, Jose M. Segui-Simarro
Summary: Vaccaria hispanica, an interesting species with attractive agronomic properties and valuable bioactive compounds, has potential applications in various fields. However, there is a lack of studies on in vitro clonal population production. This study investigates the possibilities of regenerating clonal plants from two wild Turkish genotypes through somatic embryogenesis and organogenesis, and identifies suitable explant types and plant growth regulator combinations for inducing different morphogenic processes.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
Antonio Calabuig-Serna, Ricardo Mir, Paloma Arjona, Jose Maria Segui-Simarro
Summary: In this study, the role of calcium in somatic embryogenesis in carrot was investigated. It was found that high calcium levels and the formation of callose layer are indicators of embryogenesis induction. Ca2+ patterns vary in different embryogenic structures. Modulating intracellular Ca2+ levels can effectively regulate the efficiency of somatic embryogenesis in carrot. Additionally, Ca2+-calmodulin signaling is associated with transcriptional remodeling necessary for embryo progression.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Antonio Calabuig-Serna, Ricardo Mir, Jose M. Segui-Simarro
Summary: In this study, somatic embryogenesis in Arabidopsis was induced using IZEs as explants. The process was characterized at the microscopic level, and specific aspects such as WUS expression, callose deposition, and Ca2+ dynamics were studied. The results showed that somatic embryos are produced from WUS-expressing cells in a finger-like appendix, and Ca2+ levels and callose deposition serve as early markers of the embryogenic regions. The study also found that Ca2+ homeostasis in this system is strictly maintained and cannot be altered to modulate embryo production.
Article
Plant Sciences
Antonio Calabuig-Serna, Ricardo Mir, Rosa Porcel, Jose M. Segui-Simarro
Summary: Brassica napus is a species of high agronomic interest commonly used for studying various processes, including microspore embryogenesis. DH4079 and DH12075 lines, with high and low embryogenic response respectively, are ideal for investigating the mechanisms controlling embryogenesis induction. This study examined the genetic transformation ability of DH4079 plants and compared it with the already studied DH12075 plants. Results showed that DH12075 plants responded to genetic transformation, while DH4079 plants remained recalcitrant, failing to produce any regenerant. The study also tested transient transformation with Agrobacterium rhizogenes, where only DH12075 showed a response. These findings indicate that DH4079 is recalcitrant to Agrobacterium-mediated transformation.
Article
Agronomy
Jose M. Segui-Simarro, Nathanael M. A. Jacquier, Thomas Widiez
Summary: Doubled haploids (DH) have become a powerful tool used by breeding companies to produce pure lines for hybrid seed production. The biotechnological revolution has led to the development of multiple approaches for producing haploids and DHs in various species, offering flexibility and reliability in the process.
DOUBLED HAPLOID TECHNOLOGY, VOL. 1: General Topics, Alliaceae, Cereals
(2021)
Editorial Material
Agronomy
Jose M. Segui-Simarro
DOUBLED HAPLOID TECHNOLOGY, VOL. 3: Emerging Tools, Cucurbits, Trees, Other Species
(2021)