Article
Multidisciplinary Sciences
Jack M. Moen, Kyle Mohler, Svetlana Rogulina, Xiaojian Shi, Hongying Shen, Jesse Rinehart
Summary: Protein phosphorylation is a crucial post-translational modification that regulates cellular processes and protein-protein interactions. Traditional strategies for functional assignment of phosphorylation events have become inadequate in keeping up with the rapid identification pace through proteomic surveillance. However, the development of phospho-amino-acid-specific orthogonal translation systems offers a kinase-independent method to assess the physiological function of phosphorylation.
NATURE COMMUNICATIONS
(2022)
Review
Biology
Seung-Hyeon Seok
Summary: Protein phosphorylation, a crucial post-translational modification process, is regulated by protein kinases and protein phosphatases. These reversible enzyme activities provide a regulatory mechanism for diverse protein functions in cellular processes.
Article
Chemistry, Medicinal
Dhanaji M. Lade, Roxy Nicoletti, James Mersch, Yehenew M. Agazie
Summary: SHP2 is an important oncogene in cancers driven by overexpression of receptor tyrosine kinases. In this study, we developed an optimized derivative BPDA2 as an SHP2 inhibitor with increased potency and selectivity, and demonstrated its efficacy and selectivity in cellular assays.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Julia Kamenz, Lendert Gelens, James E. Ferrell
Summary: The phosphorylation of mitotic proteins is bistable, regulated by both Cdk1 and PP2A-B55 in a bidirectional manner. The changes in PP2A-B55 activity serve as the ultimate trigger for mitotic entry and exit, indicating its crucial role in regulating cell cycle progression.
Review
Biochemistry & Molecular Biology
Hieu Nguyen, Arminja N. Kettenbach
Summary: Dynamic protein phosphorylation and dephosphorylation play critical roles in cellular signaling and biological functions. Dysregulation of these processes has been linked to various human diseases. This review focuses on the mechanisms controlling the specific dephosphorylation of proteins. The majority of serine/threonine dephosphorylation is catalyzed by highly conserved phosphoprotein phosphatase (PPP) catalytic subunits, which form holoenzymes with regulatory and scaffolding subunits. PPP holoenzymes recognize phosphorylation site consensus motifs and interact with short linear motifs (SLiMs) or structural elements distal to the phosphorylation site. Recent advances in understanding the mechanisms of PPP site-specific dephosphorylation preference and substrate recruitment are discussed, with emphasis on their roles in regulating cell division.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Shutang Tan, Christian Luschnig, Jiri Friml
Summary: The plant hormone auxin is central to plant growth and development, with protein phosphorylation emerging as a crucial post-translational modification type regulating auxin output. This review comprehensively discusses the genetics, biochemistry, and cell biology of kinases and phosphatases involved in auxin action, providing insights into the mechanisms of developmental auxin responses and future directions for integrating protein phosphorylation control into the molecular auxin network.
Article
Chemistry, Medicinal
Christian Borgo, Luca Cesaro, Tsuyoshi Hirota, Keiko Kuwata, Claudio D'Amore, Thomas Ruppert, Renata Blatnik, Mauro Salvi, Lorenzo A. Pinna
Summary: CK2, a protein kinase with pleiotropic functions, is implicated in global human pathologies, particularly cancer. Selective inhibitors like CX4945 and GO289 show promise in controlling the CK2-dependent phosphoproteome.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ilona Faustova, Kaidi Moll, Ervin Valk, Mart Loog, Mihkel Ord
Summary: Cyclins not only activate CDK complexes, but also serve as docking scaffolds for CDK substrates and inhibitors. G1-cyclins in yeast play a specific role in promoting bud growth and polarization, essential for cell survival. The discovery of a specific docking motif in G1-cyclins expands our understanding of cyclin specificity mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Maru Jaime-Garza, Carlos A. Nowotny, Daniel Coutandin, Feng Wang, Mariano Tabios, David A. Agard
Summary: PP5 requires Hsp90 to dephosphorylate CRaf kinase and Cdc37. Hsp90 acts as a platform to facilitate targeted dephosphorylation by PP5 and collaborates with phosphorylated Cdc37 for the folding and activation of client kinases. The cryo-EM structure reveals how Hsp90 activates and scaffolds PP5 to dephosphorylate nearby phosphorylation sites of CRaf, showing the role of Hsp90 in post-translational modification of client kinases goes beyond folding and activation.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Rosa D. Hernansaiz-Ballesteros, Csenge Foldi, Luca Cardelli, Laszlo G. Nagy, Attila Csikasz-Nagy
Summary: Studying the kinase-phosphatase pair Wee1 and Cdc25 revealed their reversed action on CDK, which helps establish a stable G2 phase and add checkpoints to the cell cycle. This regulatory mechanism appeared in LECA and co-evolved in eukaryotes, indicating its crucial role at the emergence of eukaryotes.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Gaifen Li, Jia Liu, Mengyuan Guo, Yakun Gu, Yuying Guan, Qianqian Shao, Wei Ma, Xunming Ji
Summary: Chronic hypoxia leads to irreversible cognitive impairment, primarily due to hippocampal neurodegeneration. The underlying mechanism of this process is poorly understood. This study found that chronic hypoxia can induce alpha-synuclein hyperphosphorylation, protein aggregation, hippocampal neurodegeneration, and cognitive deficits. It was also discovered that chronic hypoxia enhances ceramide catabolism, leading to the inhibition of protein phosphatase 2A and sustained induction of alpha-synuclein hyperphosphorylation. Intermittent hypoxic preconditioning can protect against chronic hypoxia-induced hippocampal neurodegeneration and cognitive impairment by preventing alpha-synuclein hyperphosphorylation. This research reveals a novel mechanism underlying alpha-synuclein pathology during chronic hypoxia and has implications for developing therapeutic interventions for chronic hypoxia-related brain disorders.
CELL DEATH DISCOVERY
(2022)
Article
Multidisciplinary Sciences
Danielle L. Schmitt, Stephanie D. Curtis, Anne C. Lyons, Jin-fan Zhang, Mingyuan Chen, Catherine Y. He, Sohum Mehta, Reuben J. Shaw, Jin Zhang
Summary: AMP-activated protein kinase (AMPK) is a master regulator of cellular metabolism. Researchers have developed a sensitive biosensor for AMPK and used it to uncover mechanisms for AMPK activity in the lysosome and nucleus.
NATURE COMMUNICATIONS
(2022)
Review
Immunology
Frederic Bornancin, Carien Dekker
Summary: This review focuses on the regulation of the NLRP3 inflammasome by phosphorylation and dephosphorylation, and discusses the kinases and phosphatases that have been reported to modulate NLRP3 activity. The aim is to integrate the current understanding and highlight potential gaps for further studies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Chen Su, Simon Rousseau, Amin Emad
Summary: The study identified transcriptional regulatory mechanisms involved in host cell response to SARS-CoV-2 infection, focusing on those specific to the virus when compared to other respiratory viruses. Through network-guided analyses, kinases associated with the network were identified, revealing pathways linked to inflammation and immunity that may exacerbate organ damage in COVID-19 complications.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Rong Liu, Guangxi Yang, Min Bao, Zhongmei Zhou, Xiaoyun Mao, Wenjing Liu, Xiaoyan Jiang, Di Zhu, Xinle Ren, Jian Huang, Ceshi Chen
Summary: This study identifies STAMBPL1 as a deubiquitinase of MKP-1 in breast cancer cells, which stabilizes MKP-1 protein by deubiquitination. Depletion of STAMBPL1 and MKP-1 increases breast cancer sensitivity to cisplatin and activates the JNK signaling pathway. These findings suggest that the STAMBPL1/MKP-1 axis could be a potential therapeutic target in breast cancer.
Article
Nanoscience & Nanotechnology
Jie Sun, Tanupong Rattanasawatesun, Penghao Tang, Zhaoxia Bi, Santosh Pandit, Lisa Lam, Caroline Wasen, Malin Erlandsson, Maria Bokarewa, Jichen Dong, Feng Ding, Fangzhu Xiong, Ivan Mijakovic
Summary: The study reveals that vertically oriented graphene preferentially grows in areas with stronger local electric fields during plasma-enhanced chemical vapor deposition. The electric field helps accumulate charges on graphene, transforming cohesive graphene layers into separate three-dimensional VG flakes. Additionally, the field attracts charged precursors to protruding areas on the substrate, aiding in the formation of VG.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Microbiology
Priyanka Singh, Ivan Mijakovic
Summary: Green silver nanoparticles (G-AgNPs) have garnered attention in medical science for their strong antimicrobial properties against multidrug-resistant microorganisms. This study demonstrates a simple and environmentally friendly method to produce G-AgNPs from an environmental isolate of Viridibacillus sp. The produced G-AgNPs were stable for over 1 year in an aqueous solution and exhibited remarkable antimicrobial activity against Gram-negative pathogens.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Priyanka Singh, Ivan Mijakovic
Summary: In this study, rowanberries were found to be effective in reducing gold and silver salts into nanoparticles. The produced nanoparticles were highly monodisperse, spherical, and stable over long periods. However, the antimicrobial activity of these nanoparticles was found to be low.
Review
Microbiology
Fazlurrahman Khan, Priyanka Singh, Abhayraj S. Joshi, Nazia Tabassum, Geum-Jae Jeong, Nilushi Indika Bamunuarachchi, Ivan Mijakovic, Young-Mog Kim
Summary: This review discusses the antimicrobial properties of nisin and its derivatives, as well as various strategies employed to enhance its activity and stability. The underlying mechanisms of action and evolutionary relationships of nisin genes are also explored. The review provides valuable insights into the research on nisin as an antimicrobial agent.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Review
Chemistry, Physical
Muthusankar Eswaran, Bavatharani Chokkiah, Santosh Pandit, Shadi Rahimi, Ragupathy Dhanusuraman, Mahaboobbatcha Aleem, Ivan Mijakovic
Summary: Field effect transistor (FET)-based nanoelectronic biosensors provide a viable route for cancer biomarker detection. Researchers have made significant efforts to address issues such as sensitivity, selectivity, and operational conditions to improve the sensing performance of FETs. This review discusses the fabrication of FETs, choice of bioreceptors, operational conditions, and strategies for improving the sensing performance of FET-based diagnostic devices.
Article
Biophysics
Omur Acet, Emrah Dikici, Burcu Onal Acet, Mehmet Odabasi, Ivan Mijakovic, Santosh Pandit
Summary: Microbial adhesion and formation of biofilms pose serious problems in various areas. Preventing microbial adhesion and biofilm formation is a promising strategy to address these issues. In this study, attaching EGCG to membranes effectively prevented bacterial colonization.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Pharmacology & Pharmacy
Shadi Rahimi, Daniel van Leeuwen, Fariba Roshanzamir, Santosh Pandit, Lei Shi, Nima Sasanian, Jens Nielsen, Elin K. Esbjoerner, Ivan Mijakovic
Summary: To enhance the delivery of doxorubicin (DOX) and minimize its side effects, researchers developed a pH-responsive delivery system based on graphene oxide (GO) that can release drugs specifically in acidic tumor environments.
Review
Microbiology
Pragati Rajendra More, Santosh Pandit, Anna De Filippis, Gianluigi Franci, Ivan Mijakovic, Massimiliano Galdiero
Summary: This review focuses on the synthesis of silver nanoparticles (AgNPs) and their mechanism of action against antibiotic-resistant and biofilm-forming bacteria. AgNPs exert their antibacterial effects through oxidative stress, protein dysfunction, membrane disruption, and DNA damage in bacteria. They also prevent biofilm formation by altering bacterial cell adhesion. However, the medical use of AgNPs is limited by their harmful effects on humans and the environment. The review compiles recent studies on the antibacterial activity of AgNPs and discusses their potential applications in medicine.
Article
Chemistry, Multidisciplinary
Shadi Rahimi, Teo Lovmar, Alexandra Aulova, Santosh Pandit, Martin Lovmar, Sven Forsberg, Magnus Svensson, Roland Kadar, Ivan Mijakovic
Summary: To combat bacterial infections in the post-antibiotic era, efforts are being made to engineer new materials with antibacterial properties. A new automated pipeline based on scanning electron microscopy images has been developed to predict the antibacterial potential of these materials. The density of sharp exposed edges of graphite-oriented nanoplatelets (GNPs) is a key factor in killing bacteria on contact.
Article
Energy & Fuels
Muthusankar Eswaran, Shadi Rahimi, Santosh Pandit, Bavatharani Chokkiah, Ivan Mijakovic
Summary: In this work, a flexible, multifunctional polyimide (PI)/Au-polyaniline (PAN)/Pd nanocomposite electrode with excellent electrochemical properties was fabricated. The electrode showed high conductivity, rapid response, and high electron transfer rate due to the strong metal-polymer interaction between PAN and Pd. It demonstrated successful biomedical sensing of glucose and electrooxidation of alcohols such as methanol and ethanol, making it promising for healthcare diagnostics and alternative energy conversion devices.
Article
Chemistry, Multidisciplinary
Zhejian Cao, Xiaozhi Fu, Hao Li, Santosh Pandit, Francoise M. Amombo Noa, Lars Ohrstrom, Aleksej Zelezniak, Ivan Mijakovic
Summary: This study successfully synthesized MOFs using enzymatically recycled PET, demonstrating the feasibility of this approach. Compared to conventional methods, this approach avoids the use of strong acids or bases and high temperatures, leading to reduced environmental and energy penalties.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Jian Zhang, Nisha Neupane, Puspa Raj Dahal, Shadi Rahimi, Zhejian Cao, Santosh Pandit, Ivan Mijakovic
Summary: Developed antibiotic-loaded boron nitride nanoconjugates with strong antimicrobial properties for combating bacterial biofilms.
ACS APPLIED BIO MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Hengzhi Ruan, Marko Bek, Santosh Pandit, Alexandra Aulova, Jian Zhang, Philip Bjellheim, Martin Lovmar, Ivan Mijakovic, Roland Kadar
Summary: This study presents a facile approach of incorporating dopamine and [2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide via in situ synthesis into gelatin hydrogels. The resulting hydrogels show exceptional antibacterial efficiency and multifunctional performance, making them highly promising for various biomedical applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Chemistry, Multidisciplinary
Fazlurrahman Khan, Geum-Jae Jeong, Priyanka Singh, Nazia Tabassum, Ivan Mijakovic, Young-Mog Kim
Summary: Emerging nanotechnology has successfully synthesized and applied nanoparticles using the green-chemistry approach, which offers simplicity, cost-effectiveness, environmental friendliness, stability, and biocompatibility. Researchers have turned to pure-compound-based synthesis due to the diverse and environmentally influenced composition of natural extracts, investigating the biological activities and mechanisms of these nanoparticles.
Article
Multidisciplinary Sciences
Priyanka Singh, Ivan Mijakovic
Summary: The increasing demand for green and biological nanoparticles has led to the exploration of medicinal plants and other green resources. In this study, Ligustrum vulgare berries were used to reduce gold and silver salts into nanoparticles. The resulting nanoparticles showed stability and antimicrobial properties.
SCIENTIFIC REPORTS
(2022)