Article
Food Science & Technology
Mehdi Zarei, Saeid Rahimi, Ali Fazlara, Sahar Elmi Anvari
Summary: The surfaces of poultry slaughterhouse equipment are major sources of Pseudomonas contamination, causing spoilage of poultry meat during refrigerated storage. Pseudomonas strains with high biofilm-forming ability were isolated from different surfaces, and their biofilm formation was studied. These strains produced biofilm on polystyrene microplates, and some strains were identified as P. fragi, P. fluorescens, P. lundensis, and P. cedrina. The ability of these strains to form biofilms with S. enteritidis depended on the timing of biofilm formation and the contribution of S. enteritidis.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Veterinary Sciences
A. Guerrini, G. Mescolini, P. Roncada, G. Tosi, E. Raffini, M. Frasnelli
Summary: This study aimed to investigate the prevalence of Salmonella enterica serovar Enteritidis (S.E.) and Salmonella enterica serovar Typhimurium (S. T.) in ornamental poultry in Italy. The results showed that both S.E. and S.T. were widespread in studied backyard poultry farms, with animals serving as potential reservoirs of Salmonella. It is important to inform farmers about the importance of regular and periodic control of animals, eggs, or meat to prevent the spread of these diseases.
PAKISTAN VETERINARY JOURNAL
(2021)
Article
Agriculture, Dairy & Animal Science
Xin Yu, Hongwei Zhu, Yongheng Bo, Youzhi Li, Yue Zhang, Yang Liu, Jianlong Zhang, Linlin Jiang, Guozhong Chen, Xingxiao Zhang
Summary: The study investigated the prevalence and antimicrobial resistance of Salmonella enterica serovar Enteritidis in broiler chickens in Shandong Province, China. It found that S. Enteritidis was the predominant serovar with high antimicrobial resistance rates to several antibiotics. Multidrug resistance, particularly to fluoroquinolone, showed an increasing trend from 2013 to 2018. This study provides updated information on antimicrobial resistance data and risk assessment for Salmonella Enteritidis in broiler chickens in Shandong Province, which can be valuable for future surveillance activities to ensure chicken supply safety.
Article
Agronomy
Xiaoqing Hu, Xiaoying Sun, Shuanghua Luo, Shuyan Wu, Zhaojuan Chu, Xiujuan Zhang, Zhaojun Liu, Jiaxin Wu, Xiaohong Wang, Chang Liu, Xiaoyuan Wang
Summary: The study demonstrated the effectiveness of blue light (BL) in inactivating Salmonella Enteritidis on eggshells, while maintaining eggshell freshness. BL-treated eggs showed lower weight loss, higher Haugh unit, and higher total amino acid content during storage period, indicating the advantage of BL treatment in maintaining egg quality.
Article
Agriculture, Dairy & Animal Science
Katya Arnold, Seunghyun Lim, Tal Rakler, Albert Rovira, Cinthia Satuchne, Elinor Yechezkel, Anat Wiseman, Yaniv Pima, Eugenia Yakunin, Assaf Rokney, Ehud Elnekave
Summary: Non-typhoidal Salmonella (NTS) is a global threat to public health, and poultry is one of the main reservoirs. The current methods for NTS characterization are time-consuming and have limited discriminatory power. Whole-genome sequencing can identify genetic variation within serotypes, and a multiplex PCR assay has been designed for the rapid and cost-efficient detection of emerging S. Muenchen serotype and its variants.
Article
Infectious Diseases
Mohammad Mehdi Soltan Dallal, Fatemeh Zeynali Kelishomi, Farhad Nikkhahi, Taghi Zahraei Salehi, Fatemeh Fardsanei, Amir Peymani
Summary: This study evaluated the antibiotic resistance, antibiotic resistance genes, and biofilm formation capacity of 34 Salmonella enterica serovar Entritidis strains. The genetic diversity of the strains was analyzed using PFGE. The results showed that the strains had high resistance to cefuroxime, nalidixic acid, and ciprofloxacin, and most of them were biofilm producers. Genetic analysis revealed high homogeneity among strains with the same XbaI PFGE profile, indicating the need for multiple typing methods for reliable results.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Ji-Yeon Hyeon, Shaoting Li, David A. Mann, Shaokang Zhang, Kyu-Jik Kim, Dong-Hun Lee, Xiangyu Deng, Chang-Seon Song
Summary: This study investigated 96 SE isolates from poultry sources in South Korea from 2010 to 2017, revealing the presence of various antimicrobial resistance genes and a common virulence gene profile among the isolates. Phylogenetic analysis suggested the existence of genetically related isolates in processing plants, slaughterhouses, and retail meats, indicating a potential common source for different SE strains.
Article
Infectious Diseases
Elena Circella, Gaia Casalino, Francesco D'Amico, Nicola Pugliese, Michela Maria Dimuccio, Antonio Camarda, Giancarlo Bozzo
Summary: In recent years, there has been a more cautious use of drugs on livestock farms, leading to the consideration of alternative products in order to reduce the risk of antibiotic resistance. This study aimed to evaluate the effectiveness of garlic in inhibiting Salmonella enterica subsp. enterica serovar Enteritidis and to determine its Minimal Inhibitory Concentration (MIC)(90) and MIC50 values. The results showed that garlic has the potential to inhibit the growth of S. Enteritidis in vitro, with efficacy varying depending on the microbial concentration used.
Article
Agriculture, Dairy & Animal Science
Matias Aguilera, Eduardo Tobar-Calfucoy, Victoria Rojas-Martinez, Rodrigo Norambuena, Maria Jesus Serrano, Onix Cifuentes, Maria Sofia Zamudio, Daniel San Martin, Pabla Lara, Andrea Sabag, Marcela Zabner, Daniel Tichy, Pamela Camejo, Luis Leon, Michael Pino, Soledad Ulloa, Felipe Rojas, Christian Pieringer, Cecilia Muster, Daniel Castillo, Nicolas Ferreira, Camilo Avendano, Mauro Canaval, Hans Pieringer, Pablo Cifuentes, Nicolas Cifuentes Munoz
Summary: In this study, we isolated and characterized 47 bacteriophages that showed variable degrees of lytic activity against 258 Salmonella isolates from a commercial broiler company in Brazil. Three of these bacteriophages were selected to assemble a cocktail, which was found to be highly effective against multiple serovars of Salmonella in vitro, including under conditions that resemble the chicken gut. The cocktail also showed limited or no cross-resistance, highlighting its potential for in vivo application in commercial broiler farms.
Article
Biotechnology & Applied Microbiology
Diessy Kipper, Renato H. Orsi, Laura M. Carroll, Andrea K. Mascitti, Andre F. Streck, Andre S. K. Fonseca, Nilo Ikuta, Eduardo C. Tondo, Martin Wiedmann, Vagner R. Lunge
Summary: The study assessed the population structure, recent temporal evolution, and important genetic characteristics of multidrug-resistant Salmonella enterica serovar Heidelberg isolated from Brazilian poultry farms, revealing a monophyletic group with antibiotic resistance genes and virulence factors. This lineage poses a threat to Brazilian and global poultry operations.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Amrit Pal, Montana R. Riggs, Andrea Urrutia, Rachel C. Osborne, Alexandra P. Jackson, Matthew A. Bailey, Kenneth S. Macklin, Stuart B. Price, R. Jeffrey Buhr, Dianna Bourassa
Summary: This study evaluated the impact of different levels of aerosolized Salmonella on the colonization and persistence of Salmonella in various tissues of broilers. Higher levels of challenge resulted in increased Salmonella prevalence in tissues, while the prevalence remained low and constant over time with a lower challenge level of 10(3) Salmonella.
Article
Immunology
Leonardo Saenz, Miguel Guzman, Sonia Vidal, Mario Caruffo, Daniela Siel, Caridad Zayas, Rodolfo Paredes, Carolina Valenzuela, Hector Hidalgo, Oliver Perez, Lisette Lapierre
Summary: This study evaluated the efficacy and safety of a cochleate-based trivalent injectable vaccine against three Salmonella serovars. The vaccine showed significant reduction in bacterial count and induction of neutralizing antibodies, demonstrating its effectiveness in controlling the spread of Salmonella in poultry farms.
Article
Biology
Sion C. Bayliss, Rebecca K. Locke, Claire Jenkins, Marie Anne Chattaway, Timothy J. Dallman, Lauren A. Cowley, Ben S. Cooper
Summary: This study developed a hierarchical machine learning model to rapidly identify and trace the geographical source of Salmonella enterica serovar Enteritidis infections from whole genome sequencing data. The model achieved high accuracy in predicting the continent, sub-region, and country of origin, and provides results within 4 minutes per sample, facilitating rapid outbreak resolution and real-time genomic epidemiology.
Article
Multidisciplinary Sciences
Rortana Chea, Hung Nguyen-Viet, Sothyra Tum, Fred Unger, Johanna Lindahl, Delia Grace, Chhay Ty, Sok Koam, Vor Sina, Huy Sokchea, Son Pov, Theng Heng, Or Phirum, Sinh Dang-Xuan
Summary: This study assesses the transfer of Salmonella enterica from raw chicken carcasses to ready-to-eat chicken salad in Cambodia. The results show how different hygiene practices influence the risk of pathogens contaminating chicken salad.
Article
Microbiology
Marta Kuzminska-Bajor, Paulina Sliwka, Pawel Korzeniowski, Maciej Kuczkowski, David Saez Moreno, Anna Wozniak-Biel, Emilia Sliwinska, Krzysztof Grzymajlo
Summary: The UPWr_S134 phage cocktail has shown promising results in reducing Salmonella in broiler chickens. The phages in the cocktail remain active under various conditions encountered in the chicken gastrointestinal tract and exhibit strong stability. In mouse and chicken models, the application of the UPWr_S134 phage cocktail delayed infection symptoms and reduced the number of pathogens in internal organs compared to untreated animals.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Ophthalmology
Rodrigo A. Valenzuela, Ivan Flores, Myriam Pujol, Carolina Llanos, Ester Carreno, Gabriel Rada, Carl P. Herbort, Loreto Cuitino, Cristhian A. Urzua
Summary: This review evaluates the different definitions of refractoriness in uveitis in the literature and finds a lack of consensus. Definitions of corticosteroids-refractoriness are related to persistence of inflammation and recurrences above a dosage threshold, while definitions for immunomodulatory therapy and biologic agents vary greatly.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2022)
Article
Infectious Diseases
Patricio Retamal, Pedro abalos, Raul Alegria-Moran, Nicolas Valdivieso, Martin Vordermeier, Gareth Jones, Karina Saadi, Carolina Perez Watt, Constanza Salinas, Constanza avila, Valentina Padilla, Belen Benavides, Romina Orellana
Summary: The study evaluated the efficacy of vaccinating dairy cattle with the BCG Russia strain in a high prevalence area of Chile for tuberculosis prevention. Results showed a significant reduction in infection rates and beneficial effects on milk production parameters.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Alfredo Sierra-Cristancho, Luis Gonzalez-Osuna, Julian Balanta-Melo, Emilio A. Cafferata, Carolina Rojas, Samanta Melgar-Rodriguez, Paola Carvajal, Rolando Vernal
Summary: This study describes the dentine thickness in mandibular first premolars with C-shaped root canals and identifies the areas with thinner canal walls as potential danger zones. In addition, it analyzes the internal and external anatomical characteristics of these teeth and their association with dentine thickness.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Microbiology
Dinka Ivulic, Ramon Rossello-Mora, Tomeu Viver, David A. Montero, Sonia Vidal, Francisco Aspee, Hector Hidalgo, Roberto Vidal
Summary: This study investigated the relationship between airborne and respiratory tract-associated bacterial diversity in broiler production systems. Results showed a decrease in coliforms in the air over time, but an increase was observed in week 3 due to litter management practices. There were distinct microbial communities in the air primarily associated with the Bacilli class, and the tracheal microbiome shifted from Escherichia coli/Shigella to Gallibacterium over the production cycle.
Review
Biochemistry & Molecular Biology
Angelica M. Cardenas, Laura J. Ardila, Rolando Vernal, Samanta Melgar-Rodriguez, Hernan G. Hernandez
Summary: This study investigated the epigenetic profile of periodontitis in the systemic immunity and identified several genes with differential DNA methylation and RNA expression in patients with periodontitis, which could serve as potential biomarkers for clinical use.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Luis Daniel Sansores-Espana, Samanta Melgar-Rodriguez, Rolando Vernal, Bertha Arelly Carrillo-Avila, Victor Manuel Martinez-Aguilar, Jaime Diaz-Zuniga
Summary: Periodontitis is a chronic disease caused by dysbiotic changes in the subgingival microbiota. Neutrophils play a central role in periodontal inflammation, and recent evidence suggests the presence of different neutrophil subsets. However, there is no evidence demonstrating the presence of specific neutrophil subtypes in periodontal tissues during periodontitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Roberto M. Vidal, David A. Montero, Felipe Del Canto, Juan C. Salazar, Carolina Arellano, Alhejandra Alvarez, Nora L. Padola, Hernan Moscuzza, Analia Etcheverria, Daniel Fernandez, Victoria Velez, Mauro Garcia, Rocio Colello, Marcelo Sanz, Angel Onate
Summary: In this study, the safety and immunogenicity of a vaccine candidate against Shiga toxin-producing Escherichia coli (STEC) was evaluated. The vaccine, formulated with two chimeric proteins, was administered to pregnant cows in their third trimester of gestation. The results demonstrated that the vaccine is safe and immunogenic, suggesting the feasibility of vaccinating cows with it during late pregnancy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Josefa Nunez-Belmar, Mauricio Morales-Olavarria, Emiliano Vicencio, Rolando Vernal, Juan P. Cardenas, Cristian Cortez
Summary: This review discusses the use of different -omics technologies and techniques in studying the pathogenic potential of P. gingivalis and its interaction with the host.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Dentistry, Oral Surgery & Medicine
Angelica M. Cardenas, Vanessa Campos-Bijit, Fabrizio Di Francesco, Frank Schwarz, Emilio A. Cafferata, Rolando Vernal
Summary: This systematic review analyzed 63 studies using electrolyzed water for oral disinfection, showing that electrolyzed water has high disinfection potential in the dental setting and is effective against various oral conditions. It demonstrated a broad antimicrobial spectrum, efficiently disinfecting viruses, fungi, and bacteria, and being compatible with most dental materials. Additionally, electrolyzed water can effectively protect against SARS-CoV-2 infection and contamination in dental offices.
Article
Biochemistry & Molecular Biology
Cristian Navarrete, Alejandro Riquelme, Natalia Baksai, Romina Perez, Claudia Gonzalez, Maria Michea, Hans von Muhlenbrock, Emilio A. Cafferata, Rolando Vernal
Summary: During orthodontic treatment, the production of certain substances in the periodontal tissues of teeth can affect alveolar bone remodeling and teeth movement. This study examined the production levels of certain substances in periodontal tissues of patients with reduced periodontal support undergoing orthodontic treatment. The results showed that the specific orthodontic treatment based on low-intensity intermittent forces was well tolerated by the compromised teeth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Diego Zamorano, Dinka Ivulic, Tomeu Viver, Felipe Morales, Francisco Lopez-Kostner, Roberto M. Vidal
Summary: Anastomotic leakage (AL) is a common complication after colorectal surgery and the underlying mechanism is still not well understood. This study aimed to investigate the association between gut microbiota and colorectal AL development. Through analyzing tissue-associated microbiota using 16S rRNA sequencing, lower microbial diversity was observed in the AL group compared to non-leak anastomosis (NLA) group. The facultative anaerobic bacterium Gemella palaticanis showed high abundance and may be a potential marker for AL.
Article
Dentistry, Oral Surgery & Medicine
Alfredo Torres, M. Angelica Michea, Akos Vegvari, Marion Arce, Alicia Morales, Elias Lanyon, Marcela Alcota, Camila Fuentes, Rolando Vernal, Mauricio Budini, Roman A. Zubarev, Fermin E. Gonzalez
Summary: The study aimed to explore the qualitative and quantitative differences in gingival crevicular fluid (GCF) proteome of periodontitis patients during disease progression. It was found that the GCF proteome of progressive periodontitis was associated with metabolic processes, immune response, and cellular stress, while the proteome of stable periodontitis was mainly related to wound repair, cell death regulation, and autophagy.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Review
Microbiology
Valentina B. Pavez, Nicolas Pacheco, Juan Castro-Severyn, Coral Pardo-Este, Javiera Alvarez, Phillippi Zepeda, Gabriel Kruger, Karem Gallardo, Francisco Melo, Rolando Vernal, Carlos Aranda, Francisco Remonsellez, Claudia P. Saavedra
Summary: In this study, the capacity of Exiguobacterium strains isolated from the Salar de Huasco in Chile to form biofilms in the presence of arsenic was assessed to understand their resistance mechanisms to this heavy metal. The results showed that these strains exhibit a significant ability to form biofilms when exposed to arsenic, shedding light on the underlying mechanisms. These findings enhance our understanding of biofilm formation, its role in arsenic resistance, and the adaptive strategies employed by bacteria in extreme environments.
MICROBIOLOGY SPECTRUM
(2023)
Article
Dentistry, Oral Surgery & Medicine
Fabrizio Di Francesco, Gennaro De Marco, Corina M. Cristache, Rolando Vernal, Emilio A. Cafferata, Alessandro Lanza
Summary: The use of prefabricated titanium abutments for single posterior implant-supported restorations is a clinically acceptable treatment option, with high implant and abutment survival rates and a relatively low incidence of mechanical complications.
INTERNATIONAL JOURNAL OF PROSTHODONTICS
(2022)
Article
Medicine, General & Internal
Claudia Amaya Bautista, Emilio A. Cafferata, Rolando Vernal, Angelica M. Cardenas
Summary: In this case report, the clinical results of a root-covering procedure using the double papilla technique combined with a partially epithelialized connective tissue graft in the treatment of gingival recession in an upper canine were shown after a 12-month follow-up. The technique demonstrated highly successful results in terms of 100% coverage of the defect, long-term stability, and aesthetics.
SAGE OPEN MEDICAL CASE REPORTS
(2022)
