Article
Chemistry, Multidisciplinary
Sihan Zhang, Zhichao Liu, Zhonghui Zhang, Weikang Wang, Yang Tian
Summary: We developed a high-density intra/extracellular optophysiology platform and two probes for simultaneous quantification of intracellular cysteine and extracellular dopamine in the freely moving animal brain. Using this tool, we discovered that intracellular cysteine regulates extracellular dopamine secretion through the expression of tyrosine hydroxylase in the depressed mice brain. Additionally, we established functional networks of cysteine and dopamine across 32 brain regions in freely moving animals and found that depression reduced the correlations between adjacent brain regions, which could be recovered by treatment with N-acetyl-L-cysteine.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biotechnology & Applied Microbiology
Zhengjie Liu, Dongxin Xu, Jiaru Fang, Qijian Xia, Wenxi Zhong, Hongbo Li, Zhanyun Huang, Nan Cao, Xingxing Liu, Hui-Jiuan Chen, Ning Hu
Summary: This study developed an integrated electrical signal recording and electrical pulse regulating system based on NPMEA, which can achieve high-throughput detection of intracellular electrical signals and provide a reliable recording tool for cell electro-physiological investigation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biophysics
Yang Yang, Aofei Liu, Ching -Ting Tsai, Chun Liu, Joseph C. Wu, Bianxiao Cui
Summary: The unintended binding of small-molecule drugs to ion channels can cause arrhythmia and heart failure. However, the low throughput of current intracellular recording techniques limits the assessment of drug toxicity. To address this challenge, we have developed the NcEA technology, which enables robust, parallel, and long-duration recording of intracellular action potentials. This technology has the potential to be used in preclinical drug screening.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Multidisciplinary Sciences
Zeinab Jahed, Yang Yang, Ching-Ting Tsai, Ethan P. Foster, Allister F. McGuire, Huaxiao Yang, Aofei Liu, Csaba Forro, Zen Yan, Xin Jiang, Ming-Tao Zhao, Wei Zhang, Xiao Li, Thomas Li, Annalisa Pawlosky, Joseph C. Wu, Bianxiao Cui
Summary: This study develops mechanically robust nanocrown electrodes with high success rates achieved through electroporation, and validates their accurate recording of intracellular action potentials through simultaneous patch clamp recording. The study also demonstrates the ability of nanocrown electrodes to enable prolonged recording of the same cells after sequential addition of drugs. This technological advancement is significant for establishing a screening assay for preclinical evaluation of drug-induced arrhythmogenicity.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Yasutoshi Jimbo, Daisuke Sasaki, Takashi Ohya, Sunghoon Lee, Wonryung Lee, Faezeh Arab Hassani, Tomoyuki Yokota, Katsuhisa Matsuura, Shinjiro Umezu, Tatsuya Shimizu, Takao Someya
Summary: An organic electrochemical transistor (OECT) matrix is shown for intracellular action potential recording, where the driving voltage simultaneously causes electroporation. The recorded peak amplitudes were larger than extracellular field potential recording, and could be further enhanced by tuning the driving voltage and OECT geometry. Miniaturization and multiplexed recording capabilities were demonstrated through a 4 x 4 action potential mapping using a matrix of 5- x 5-mu m(2) OECTs, realized with a mild fabrication process and simple circuit without limiting potential applications of organic electronics.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Analytical
Juan Qiao, Deyu Wu, Yuying Song, Wenliang Ji, Qingwei Yue, Lanqun Mao, Li Qi
Summary: A new fluorescent probe was designed to simultaneously measure intracellular temperature and norepinephrine (NE) variations, revealing an increase in cell temperature and decrease in NE reuptake during antidepressant drug inhibition. This study provides insight into the underlying mechanisms of neurotransmitter signaling pathways, which could aid in the treatment of depression.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Dongxin Xu, Jiaru Fang, Hao Wang, Xinwei Wei, Jinhu Yang, Hongbo Li, Tao Yang, Ying Li, Chuan Liu, Ning Hu
Summary: In this study, a nanotrapped microelectrode based on a porous PET membrane was developed to improve cell-electrode coupling and intracellular recording performance. The nanotraps and nanoedge structure enhance the protrusion of the cell membrane and provide effective electroporation, allowing for longer intracellular access and consecutive electroporation events.
Article
Engineering, Biomedical
Yu Huan, Jeffrey P. Gill, Johanna B. Fritzinger, Paras R. Patel, Julianna M. Richie, Elena Della Valle, James D. Weiland, Cynthia A. Chestek, Hillel J. Chiel
Summary: Carbon fiber electrodes (CFEs) show potential for stable intracellular stimulation and recording with good signal-to-noise ratios, allowing for multi-neuron recording simultaneously. Despite lower amplitudes compared to glass microelectrodes, CFEs provide a powerful new technology for studying neural circuit dynamics.
JOURNAL OF NEURAL ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Jiaru Fang, Dongxin Xu, Hao Wang, Jin Wu, Ying Li, Tao Yang, Chuan Liu, Ning Hu
Summary: Electrophysiology is a unique biomarker for disease investigation or drug assessment in electrogenic cells. Vertical nanoelectrode arrays have been successful in achieving high-quality intracellular electrophysiological study in electrogenic cells. However, achieving high success rate and high-quality, long-term intracellular recording using inexpensive nanostructures remains a challenge. In this study, a scalable and robust hollow nanopillar electrode was developed to enhance intracellular recording of cardiomyocytes. Regulation of the nanostructure geometry proved to be a powerful strategy in enhancing intracellular recording.
Article
Chemistry, Multidisciplinary
Dongxin Xu, Jiaru Fang, Mingyue Zhang, Qijian Xia, Hongbo Li, Ning Hu
Summary: New strategies for intracellular electrophysiology using nanotemplate electrodes provide sensitive and prolonged recording of action potentials, leading to significant advances in the field of cardiology and neuroscience.
Article
Chemistry, Multidisciplinary
M. E. Martinez-Cartagena, J. Bernal-Martinez, M. Roman-Aguirre, A. Aguilar-Elguezabal
Summary: This study demonstrates the feasibility of using copolymer EDOT-pyrrole-coated electrodes for neural intracellular recordings. The electrical characterization of the coated electrodes was crucial to demonstrate their stability in the electrolytic medium. Recordings showed that the electrodes made of electrophoretic deposition of EDOT-PY 0.9-0.1/PO4 copolymer are sensitive to bioelectrical signals, have a signal amplitude almost two times compared to Ag/AgCl electrodes, and have a high signal-to-noise ratio (SNR = 15 dB) compared to Ag/AgCl electrodes (SNR = 11 dB). These characteristics make it a prominent material for applications in electrophysiology and neurophysiology.
MATERIALS TODAY CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yuxiao Mei, Qi-Wei Zhang, Qingyi Gu, Zhichao Liu, Xiao He, Yang Tian
Summary: This article describes a new supramolecular fluorescent probe that efficiently identifies largescale neurotransmitters and enables high-throughput analysis using principal component analysis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biophysics
Dongxin Xu, Jiaru Fang, Moran Yadid, Mingyue Zhang, Hao Wang, Qijian Xia, Hongbo Li, Nan Cao, Tal Dvir, Ning Hu
Summary: Intracellular recording of action potentials is crucial for studying disease mechanisms and electrophysiological studies. Current strategies include 3D nanoelectrodes and transient electroporation, but both have limitations. In this study, an advanced cell-based biosensing platform using electroporation is proposed, which provides consistent, high-quality recordings and allows real-time feedback.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Dongxin Xu, Jiaru Fang, Mingyue Zhang, Hao Wang, Tao Zhang, Tian Hang, Xi Xie, Ning Hu
Summary: This study introduces a novel platform that achieves synchronized intracellular and extracellular recordings of neonatal rat cardiomyocytes by low-voltage three-dimensional nanoroded electroporation, enabling high-throughput large-scale synchronous electrophysiological studies. Recorded signals contain typical EAPs and IAPs with good synchronicity in spatiotemporal dimensions, bridging the correlation between both signals in experimental and simulated ways.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Neurosciences
Jonathan Sackner-Bernstein
Summary: The study investigated intracellular dopamine levels in Parkinson's disease using meta-analytic methods. Results showed that intracellular dopamine levels in Parkinson's patients are not significantly reduced compared to normal individuals. This highlights the importance of modulating VMAT2, neuromelanin, and dopamine synthesis as potential neuroprotective strategies.
JOURNAL OF PARKINSONS DISEASE
(2021)
Article
Neurosciences
Ryan P. Silk, Hanagh R. Winter, Ouria Dkhissi-Benyahya, Carmella Evans -Molina, Alan W. Stitt, Vijay K. Tiwari, David A. Simpson, Eleni Beli
Summary: This study investigates whether diabetes affects the daily rhythm of gene expression in the retina. The results show that diabetic mice exhibited phase advancement in the expression of certain genes compared to non-diabetic mice. The study also identified oxygen-sensing mechanisms and HIF1alpha as potential upstream regulators. These findings provide important insights into the development of diabetic retinopathy.
Article
Neurosciences
Krishnamachari S. Prahalad, Daniel R. Coates
Summary: Visual stimuli presented around the time of a saccade can be perceived differently by the visual system, including a reduction in the harmful impact of flankers. This study investigated the effects of microsaccades on crowded stimuli placed 20 arc minutes from the center of gaze. The findings suggest two separate pre-saccadic benefits, one that regularizes the crowding zone and another that specifically benefits microsaccade targets surrounded by tangential flankers.
Article
Neurosciences
Chandrika Ravisankar, Christopher W. Tyler, Clifton M. Schor, Shrikant R. Bharadwaj
Summary: This study revealed that less than one-third of adults with normal binocular vision were able to successfully free-fuse random-dot image pairs and identify the embedded stereoscopic shapes. The successful participants showed a dissociation of vergence and accommodative responses, while the unsuccessful ones either exhibited strong vergence and accommodation or weak vergence and strong accommodation. Task performance of the unsuccessful cluster improved significantly with pharmacological paralysis of accommodation. A minority of participants also learned to dissociate one direction of their vergence and accommodation crosslinks with repeated free-fusion trials, optimizing their task performance.
