Article
Virology
Zhitao Wang, Yangyang Qiao, Zhijie Chen, Yumeng Liang, Lu Cui, Yanhui Zhang, Xuefeng Li, Li Xu, Ping Wei, Shengwang Liu, Hai Li
Summary: The study identified Fos as a key regulator of ILTV infection through its transcription factor function on both the virus and host sides, assisting in the restoration of viral gene transcription and viral production.
Article
Virology
Bo Yang, YongXuan Yao, Han Cheng, Xian-Zhang Wang, Yue-peng Zhou, Sheng-Nan Huang, Xuehui Ma, Hong Yang, Jinpeng Wu, Xuan Jiang, Shuang Cheng, Jin-Yan Sun, Wen-Bo Zeng, Jason Chen, Fu-Kun Zhang, Hong-Jie Shen, Jian-Yang Gu, Michael A. McVoy, William J. Britt, Sitang Gong, Min-Hua Luo
Summary: The host protein WDR11 plays a crucial role in the formation of the virion assembly compartment (vAC) and virion morphogenesis during human cytomegalovirus (HCMV) infection.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Bo Yang, YongXuan Yao, Hui Wu, Hong Yang, Xue-Hui Ma, Dong Li, Xian-Zhang Wang, Sheng-Nan Huang, Xuan Jiang, Shuang Cheng, Jin-Yan Sun, Zhen-Li Huang, CongJian Zhao, Michael A. McVoy, Jin-Hyun Ahn, Wen-Bo Zeng, William J. Britt, Sitang Gong, Min-Hua Luo
Summary: WDR5 is localized to the vAC during HCMV infection and incorporated into virions, potentially contributing to efficient virion maturation.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Widaliz Vega-Rodriguez, Nagendraprabhu Ponnuraj, Maricarmen Garcia, Keith W. Jarosinski
Summary: Studying interindividual spread of Marek's disease herpesvirus in chickens revealed that the functional role of glycoprotein C (gC) varies depending on virus genera (Mardivirus vs. Iltovirus) rather than host species (Gallid vs. Meleagrid). The study highlights the conservation of gC functionality across different virus genera.
Article
Immunology
Naazneen Ibtehaj, Afrin Bahauddin, Maxim Ivannikov, Erik Rytting, Mohammad Jamaluddin, Yuejin Liang, Jiaren Sun, Sherry L. Haller, Xiaorong Wu, Ruksana Huda
Summary: This study compared the efficacy of unique sets of monoclonal antibody-siRNA conjugates, either individually or in combination, against different subsets of B cells in a mouse model of Myasthenia gravis. The combination treatment significantly improved the clinical symptoms and could potentially be a new therapeutic approach for MG.
JOURNAL OF AUTOIMMUNITY
(2023)
Article
Microbiology
Francesca Oberto, Emanuele Carella, Claudio Caruso, Pier Luigi Acutis, Davide Lelli, Luigi Bertolotti, Loretta Masoero, Simone Peletto
Summary: The aim of this study was to develop a qualitative PCR assay that allows the discrimination of BuHV-1, BoHV-1 and BoHV-5. Specific nucleotide sequences of BuHV-1, BoHV-1 and BoHV-5 were identified through the alignment of homologous genes. The design of the primers and the optimization of the PCR assay were focused on the target sequences located on the portions of gD, gE and gG genes. This qualitative PCR assay allowed the differentiation of BoHV-1 and BoHV-5 infections in both cattle and water buffaloes, as well as heterologous BuHV-1 infections in bovine.
Review
Virology
Soumya Sucharita, Akshaya Krishnagopal, Sylvia van Drunen van den Hurk
Summary: This review provides a comprehensive analysis of the roles of specific tegument proteins in capsid transport and virion morphogenesis of Herpesviridae, with some proteins released into the cytoplasm after virus entry and others remaining associated with the capsid for transport and envelopment.
Article
Immunology
Alireza Milani, Elnaz Agi, Mohammad Hassan Pouriayevali, Mahdieh Motamedi-Rad, Fatemeh Motevalli, Azam Bolhassani
Summary: In this study, researchers investigated the immune responses of mice immunized with dendritic cells loaded with HIV-1 proteins. The results showed that using Hsp27 as an adjuvant and a heterologous DC prime/protein boost regimen significantly improved immune responses. This finding suggests that the DC Hsp27-Nef prime/protein Hsp27-Nef boost regimen may be a promising candidate for HIV-1 vaccine development.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Juan S. Rey, Wen Li, Alexander J. Bryer, Hagan Beatson, Christian Lantz, Alan N. Engelman, Juan R. Perilla
Summary: Transmission electron microscopy is widely used in biomedical imaging for discerning ultrastructure morphology at the nanometer scale, with a particular focus on visualizing virus particles. A newly developed RCNN backbone shows promising results in identifying and classifying HIV-1 virions at different stages of maturation and morphogenesis, outperforming common RCNN backbones.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Insun Kook, Joseph M. Ziegelbauer
Summary: The study demonstrates that MCPIP1 inhibits KSHV infection and suppresses viral miRNA biogenesis by directly degrading KSHV pre-miRNAs, which is related to its substrate specificity and binding affinity. Additionally, MCPIP1 can reduce the expression of Dicer to restrict KSHV infection.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Virology
Michaela Prochazkova, Tibor Fuzik, Danyil Grybchuk, Vyacheslav Yurchenko, Pavel Plevka
Summary: This study presents the structure of Leishmania RNA virus 1 (LRV1), revealing that its capsid is built from dimers and exhibits icosahedral symmetry. Additionally, the location of the RNA polymerase within the viral particle varies.
Article
Fisheries
Carolina Gallardo-Ybarra, Arturo Sanchez-Paz, Trinidad Encinas-Garcia, Christian Minjarez-Osorio, Adriana Muhlia-Almazan, Ariel Cruz-Villacorta, Jose Manuel Grijalva-Chon, Enrique De La Re Vega
Summary: This study demonstrates that silencing the DNA polymerase gene of OsHV-1 using shRNA can delay the mortality of infected Pacific oysters, reduce apoptotic levels and decrease viral load. This suggests that shRNA may be a promising approach for controlling OsHV-1 infection in the future.
AQUACULTURE INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Rachel M. Miller, Rachel A. Knoener, Bayleigh E. Benner, Brian L. Frey, Mark Scalf, Michael R. Shortreed, Nathan M. Sherer, Lloyd M. Smith
Summary: Through proteomic analysis of HIV-1 virions, novel post-translational modifications of highly conserved residues within the viral matrix and capsid proteins were discovered, forming dehydroamino acids dehydroalanine and dehydrobutyrine. These rare and understudied dehydroamino acids may play an important role in viral particle maturation and provide valuable insights into HIV infectivity mechanisms.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Multidisciplinary Sciences
Jerry Joe E. K. Harrison, Dario Oliveira Passos, Jessica F. Bruhn, Joseph D. Bauman, Lynda Tuberty, Jeffrey J. DeStefano, Francesc Xavier Ruiz, Dmitry Lyumkis, Eddy Arnold
Summary: In this study, the cryo-electron microscopy structure of stable HIV-1 Pol was determined, revealing that RT in the Pol has a similar arrangement to the mature RT heterodimer. The dimerization of RT may activate proteolytic processing by bringing two PR monomers together. This finding suggests that HIV-1 leverages the dimerization interfaces in Pol to regulate assembly and maturation of polyprotein precursors.
Article
Microbiology
Huichao Huang, Jun Zhao, Ting-Yu Wang, Shu Zhang, Yuzheng Zhou, Youliang Rao, Chao Qin, Yongzhen Liu, Yongheng Chen, Zanxian Xia, Pinghui Feng
Summary: The study found that mouse RIG-I is resistant to evasion by HSV-1, while the N495 of human RIG-I determines species-specific deamidation by HSV-1.
Article
Virology
Mohamed S. H. Hassan, Ahmed Ali, Motamed Elsayed Mahmoud, Danah Altakrouni, Shahnas M. Najimudeen, Mohamed Faizal Abdul-Careem
Summary: This study compared the efficacy of different vaccination programs against Canadian DMV/1639 IBV and found that the autogenous inactivated DMV/1639 vaccine was more effective in reducing viral loads and boosting virus neutralization titers. Both vaccination programs provided significant protection against pathological effects.
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.
Article
Virology
Xiao Han, Hankun Xu, Yifan Weng, Rong Chen, Jidong Xu, Tong Cao, Renjie Sun, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: Classical swine fever virus (CSFV) can inhibit the host innate immunity by decreasing the level of high mobility group box 1 (HMGB1), and CSFV Npro can evade host antiviral immune response through upregulating HMGB1 acetylation.
Article
Virology
Sunjian Lyu, Fulei Xiong, Tianpeng Qi, Weifeng Shen, Qi Guo, Mingming Han, Li Liu, Weishao Bu, Julin Yuan, Bao Lou
Summary: This study identified and characterized a novel temperate A. hydrophila phage, P05B, which has strong lytic ability and stability across different temperatures and pH values.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Virology
Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang
Summary: Coxsackievirus A6 (CVA6) has emerged as an important agent causing hand, foot, and mouth disease (HFMD). Mutations in residue 64 of the virus have been found to increase resistance to ribavirin and decrease sensitivity to guanidine hydrochloride. This mutation also leads to increased replication fidelity and reduced pathogenicity. These findings provide a foundation for the development of a safe and effective live attenuated CVA6 vaccine.
Article
Virology
Yao Xiong, Keran Ma, Xiao Zou, Yantao Liang, Kaiyang Zheng, Tiancong Wang, Hong Zhang, Yue Dong, Ziyue Wang, Yundan Liu, Hongbing Shao, Andrew Mcminn, Min Wang
Summary: This study isolated a novel virus, vB_VviC_ZQ26, infecting Vibrio cyclitrophicus from coastal waters near Qingdao, China. It was found that vB_VviC_ZQ26 belongs to a new vibriophage-specific family, Coheviridae, and is mainly found in temperate and tropical epipelagic zones.
Article
Virology
Xing Li, Zhiping Ye, Ewan P. Plant
Summary: By analyzing clinical and cultured samples using the same bioinformatic pipeline, it was found that 5' copyback DVGs are prevalent in human clinical samples but not in cultured samples. Furthermore, there are differences in DVG production and composition between in vivo and in vitro infections.
Article
Virology
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Suvrotoa Mitra, Animesh Gope, Mamta Chawla-Sarkar
Summary: Rotavirus is a major cause of viral gastroenteritis in infants and continues to be a severe issue in underdeveloped nations. Recent research has shown that a long non-coding RNA called SLC7A11-AS1 is upregulated during rotavirus infection and plays a role in facilitating the virus's propagation by regulating intracellular glutathione and lipid peroxidation.
Article
Virology
Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
Summary: Bat-borne emerging viruses, such as the Ebola virus and Nipah virus, pose a significant threat to public health. In this study, we identified micafungin as a potential antiviral drug against Pteropine orthoreovirus (PRV) and found that it inhibits viral release in human cells through activating the endoplasmic reticulum stress response pathway. This research provides valuable insights into the development of effective treatments for future virus pandemics.
Article
Virology
M. Joubert, N. van den Berg, J. Theron, V. Swart
Summary: This study investigated the host gene expression in asymptomatic avocado nursery trees infected with Avocado sunblotch viroid (ASBVd) using RNA sequencing. The results showed that 631 genes were differentially expressed during infection, with 63% upregulated. ASBVd infection significantly affected plant defence responses, phytohormone networks, gene expression pathways, secondary metabolism, cellular transport, as well as protein modification and degradation. This study provides insights into the molecular interactions underlying avsunviroid-host interactions.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.
Article
Virology
Manel Essaidi-Laziosi, Francisco J. Perez-Rodriguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A. Mueller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
Summary: This study found that the Omicron variant has different replication characteristics compared to other variants of concern, being able to efficiently produce infectious virus in nasal cells but not lung cells. In addition, the Delta and Omicron variants showed increased infection efficiency in A549 cells. These findings suggest that the variants of concern have better adaptation to humans but do not have an extended host range.
Article
Virology
Martin Faye, Modeste Name Faye, Babacar Ndiaye, Moussa Moise Diagne, Safietou Sankhe, Ndeye Marieme Top, Amadou Diallo, Cheikh Loucoubar, Ndongo Dia, Amadou Alpha Sall, Ousmane Faye
Summary: Genomic surveillance in Senegal since March 2020 has detected the emergence of Omicron BA.4 and BA.5 sublineages in June 2022. Next-generation sequencing and phylogeny analysis of isolates from a cluster of cases in Northern Senegal in July 2022 revealed that the BA.4 cases originated from a XAS recombinant, marking the first reported sequence of this variant from Senegal. Continuous genomic surveillance of positive SARS-CoV-2 samples is crucial.