Journal
VIRUS RESEARCH
Volume 160, Issue 1-2, Pages 32-39Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2011.04.031
Keywords
FIPV; Entry; Monocytes; Aminopeptidase N; DC-SIGN
Categories
Funding
- Ghent University [01D29005]
- Hannah L. Dewerchin by the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT Vlaanderen)
Ask authors/readers for more resources
Two potential receptors have been described for the feline infectious peritonitis virus (FIPV): feline aminopeptidase N (fAPN) and feline dendritic cell-specific intercellular adhesion molecule grabbing nonintegrin (fDC-SIGN). In cell lines, fAPN serves as a receptor for serotype II, but not for serotype 1 FIPV. The role of fAPN in infection of in vivo target cells, monocytes, is not yet confirmed. Both serotype 1 and II FIPVs use fDC-SIGN for infection of monocyte-derived cells but how is not known. In this study, the role of fAPN and (DC-SIGN was studied at different stages in FIPV infection of monocytes. First, the effects of blocking the potential receptor(s) were studied for the processes of attachment and infection. Secondly. the level of co-localization of FIPV and the receptors was determined. It was found that FIPV I binding and infection were not affected by blocking fAPN while blocking MC-SIGN reduced FIPV I binding to 36% and practically completely inhibited infection. Accordingly, 66% of bound FIPV I particles co-localized with MC-SIGN. Blocking fAPN reduced FIPV II binding by 53% and infection by 80%. Further, 60% of bound FIPV II co-localized with fAPN. MC-SIGN was not involved in FIPV II binding but infection was reduced with 64% when (DC-SIGN was blocked. In conclusion, FIPV I infection of monocytes depends on (DC-SIGN. Most FIPV I particles already interact with MC-SIGN at the plasma membrane. For FIPV II, both fAPN and MC-SIGN are involved in infection with only fAPN playing a receptor role at the plasma membrane. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available