Journal
VIRUS RESEARCH
Volume 158, Issue 1-2, Pages 33-36Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2011.03.001
Keywords
Porcine reproductive and respiratory; syndrome virus; Defective interfering RNA
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Funding
- National Science Foundations of China [30972167, 30571390]
- Chinese Universities [2010KLEP004]
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In the present study, seven new defective interfering (DI) RNA species of porcine reproductive and respiratory syndrome virus (PRRSV) were identified. RT-PCR, Northern blot and sequence analyses indicated that these DI RNA specie have deletions of 8513-9176 nucleotides located between Nsp1/Nsp2 and Nsp10. Compared with the previous DI RNAs of PRRSV reported, they have three distinct characteristics: much smaller deletion sizes; different nucleotide repeats (2-12 nt) used in the junction sites and in-frame deletions. The results further suggested that the similarity-assisted RNA recombination may be the main cause of generation of DI RNAs in PRRSV and probably in other arteriviruses. (C) 2011 Elsevier B.V. All rights reserved.
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