Article
Biochemistry & Molecular Biology
Akash Kumar, Preeti Hooda, Anindita Puri, Radhika Khatter, Mohammed S. Al-Dosari, Neha Sinha, Mohammad K. Parvez, Deepak Sehgal
Summary: Hepatitis E Virus (HEV) is a RNA virus that requires modulation of specific enzymes for infection prevention. Methotrexate and compound A have been found to inhibit the activity of HEV helicase, thereby inhibiting HEV RNA replication.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Immunology
Pierre Cappy, Quentin Lucas, Nakourogou Kankarafou, Camille Sureau, Syria Laperche
Summary: Following a study demonstrating the role of hepatitis C virus (HCV) in transmitting hepatitis D virus (HDV) in a liver-humanized mouse model, a large cohort of HCV-positive blood donors were screened for HDV markers. However, no evidence of HCV-assisted propagation of HDV in humans was found.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Virology
Susannah Stephenson-Tsoris, John L. Casey
Summary: This study identified the start site for HDV genome RNA synthesis as position 1646 and revealed some differences in the synthesis process. The findings are important for targeting virus replication.
JOURNAL OF VIROLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mingxiao Chen, Yi Xu, Ni Li, Ping Yin, Qing Zhou, Shengjun Feng, Tiantian Wu, Lai Wei, Haihe Wang, Yongshui Fu, Yi-Ping Li
Summary: Researchers successfully developed a full-length infectious clone and a subgenomic replicon for HCV genotype 3, providing new tools for the study of the virology and pathogenesis of this genotype.
JOURNAL OF GENERAL VIROLOGY
(2021)
Review
Oncology
Yutaka Tsutsumi, Shinichi Ito, Souichi Shiratori, Takanori Teshima
Summary: The hepatitis C virus (HCV) can lead to liver cancer and is associated with lymphoma. Advances in drug treatment have improved outcomes, with the potential for HCV eradication using direct acting antivirals (DAA). HCV reactivation during lymphoma treatment and the role of HCV-RNA as a biomarker are also explored in this review.
Article
Biophysics
Akhilesh Babu Ganganboina, Kenshin Takemura, Wenjing Zhang, Tian-Cheng Li, Enoch Y. Park
Summary: The study successfully developed a viral capsid-nanoparticle hybrid structure by incorporating quantum dots into virus-like particles for HEV antibody detection. The newly developed method provides an efficient route for highly sensitive and specific antibody detection in clinical diagnosis research, utilizing QDs-encapsulated VLPs as useful fluorescence emitters for biosensing platform construction.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Cell Biology
Woan-Ing Twu, Ji-Young Lee, Heeyoung Kim, Vibhu Prasad, Berati Cerikan, Uta Haselmann, Keisuke Tabata, Ralf Bartenschlager
Summary: Although the viral DMVs are structurally similar to autophagosomes, traditional macroautophagy is not essential for the replication of HCV and SARS-CoV-2. These viruses instead utilize factors involved in autophagosome formation, particularly class III phosphatidylinositol 3-kinase (PI3K).
Article
Multidisciplinary Sciences
Payuda Hansoongnern, Pornpitra Pratedrat, Pornjarim Nilyanimit, Rujipat Wasitthankasem, Nawarat Posuwan, Nasamon Wanlapakorn, Kanchanok Kodchakorn, Prachya Kongtawelert, Napaporn Pimsing, Yong Poovorawan
Summary: Hepatitis C virus (HCV) is a viral pathogen that causes chronic hepatitis and can lead to cirrhosis and hepatocellular carcinoma. The detection of HCV RNA is the standard method for diagnosis and monitoring of antiviral treatment. A study investigated the correlation between HCV RNA and HCVcAg levels, as well as the impact of amino acid sequence heterogeneity on HCVcAg quantification. The findings showed a strong positive correlation between HCV RNA and HCVcAg, but samples with certain genotypes and amino acid substitutions exhibited lower HCVcAg levels than expected based on HCV RNA values.
Review
Biochemistry & Molecular Biology
Ja Yeon Kim Chu, Jing-hsiung James Ou
Summary: HCV utilizes cellular autophagy pathway to promote its replication, regulating autophagy through various mechanisms to control intracellular protein and membrane trafficking, thereby enhancing replication and suppressing host innate immune responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Chun-yang Gan, Jing Cui, Wen-lu Zhang, Yu-wei Wang, Ai-long Huang, Jie-li Hu
Summary: Recombinant DNA technology is used to engineer exogenous genes into the HBV genome, with potential implications for disrupting the formation of HBV's relaxed circular DNA through the induction of adverse secondary structures. This study provides valuable insights into the construction and optimization of recombinant HBV, highlighting the potential role of DNA sequence in selective pressure during HBV evolution.
FRONTIERS IN MICROBIOLOGY
(2021)
Editorial Material
Biology
Altaira D. Dearborn, Ashish Kumar, Joseph Marcotrigiano
Summary: A domain in the ORF1 polyprotein of the hepatitis E virus, previously believed to be a protease, is actually a zinc-binding domain.
Article
Multidisciplinary Sciences
E. Heilmann, J. Kimpel, B. Hofer, A. Roessler, I. Blaas, L. Egerer, T. Nolden, C. Urbiola, H. G. Kraeusslich, G. Wollmann, D. von Laer
Summary: This study presents a regulatory switch for RNA viruses that allows control over viral activity using clinically approved HIV protease inhibitors.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Mark Anderson, Jeffery Gersch, Ka-Cheung Luk, George Dawson, Ivana Carey, Kosh Agarwal, Pir Shah, Geoffrey Dusheiko, Daryl Lau, Gavin Cloherty
Summary: The study found that full-length pregenomic RNA is the primary source of circulating HBV RNA in infected patients, clarifying the major identity of HBV RNA species in circulation.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Marylin Rheault, Sophie E. Cousineau, Danielle R. Fox, Quinn H. Abram, Selena M. Sagan
Summary: In this study, the different roles of miR-122 in the HCV life cycle were isolated and evaluated using mutations, mutant miRNAs, and HCV luciferase reporter RNAs. The results indicate that the riboswitch has minimal contribution, while genome stability and translational promotion have similar contributions in the establishment phase of infection. However, in the maintenance phase, translational promotion becomes the dominant role. Additionally, an alternative conformation of the 5' untranslated region, termed SLIIalt, was found to be important for efficient virion assembly.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Microbiology
Anna V. Bulankina, Rebecca M. Richter, Christoph Welsch
Summary: Positive-strand RNA viruses, such as HCV, manipulate the lipid metabolism of infected cells and modify intracellular membranes to support their replication. Phospholipids play crucial roles in HCV replication and in the interactions between lipids and viral proteins.
Article
Biochemistry & Molecular Biology
Koudai Kawano, Aurelien J. Doucet, Mikinori Ueno, Ryusho Kariya, Wenfeng An, Flavia Marzetta, Misao Kuroki, Priscilla Turelli, Sayaka Sukegawa, Seiji Okada, Klaus Strebel, Didier Trono, Yasuo Ariumi
NUCLEIC ACIDS RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Shinya Satoh, Daichi Onomura, Youki Ueda, Hiromichi Dansako, Masao Honda, Shuichi Kaneko, Nobuyuki Kato
BIOCHEMICAL JOURNAL
(2019)
Article
Biochemistry & Molecular Biology
Mikinori Ueno, Masato Nogawa, Rokeya Siddiqui, Koichi Watashi, Takaji Wakita, Nobuyuki Kato, Masanori Ikeda, Takasi Okimura, Shogo Isaka, Tatsuya Oda, Yasuo Ariumi
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Biochemistry & Molecular Biology
Youki Ueda, Weilin Gu, Hiromichi Dansako, Hironori Nishitsuji, Shinya Satoh, Kunitada Shimotohno, Nobuyuki Kato
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Virology
Nobuyuki Kato, Youki Ueda, Hiroe Sejima, Weilin Gu, Shinya Satoh, Hiromichi Dansako, Masanori Ikeda, Kunitada Shimotohno
ARCHIVES OF VIROLOGY
(2020)
Article
Genetics & Heredity
Naoki Yamamoto, Yasuo Ariumi, Nao Nishida, Rain Yamamoto, Georg Bauer, Takashi Gojobori, Kunitada Shimotohno, Masashi Mizokami
Review
Biochemistry & Molecular Biology
Naoki Yamamoto, Rain Yamamoto, Yasuo Ariumi, Masashi Mizokami, Kunitada Shimotohno, Hiroshi Yoshikura
Summary: Individuals with comorbidities are more susceptible to severe cases of COVID-19 after infection with SARS-CoV-2, possibly due to genetic polymorphisms such as those of the angiotensin-converting enzyme 1 (ACE1) gene, which may increase their risk. However, there is still debate surrounding the association between ACE1 D/I polymorphism and comorbidities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Hiromichi Dansako, Youki Ueda, Shinya Satoh, Nobuyuki Kato
Summary: This study showed that HBV infection activated the ATM-Chk2 signaling pathway in certain human hepatocyte cells, and proposed a novel model that EVs mediate this activation by transferring mtDNA intercellularly.
Article
Virology
Yasuo Ariumi
Summary: SARS-CoV-2 infection is regulated by host cellular RNA helicases, with DDX21 and MOV10 limiting viral infection and replication, and DDX1, DDX5, and DDX6 being required for SARS-CoV-2 infection. The virus disrupts P-body formation and attenuates or suppresses stress granule formation. SARS-CoV-2 hijacks host cellular RNA helicases to facilitate viral infection and replication while suppressing the host innate immune system.
JOURNAL OF VIROLOGY
(2022)
Review
Genetics & Heredity
Naoki Yamamoto, Nao Nishida, Rain Yamamoto, Takashi Gojobori, Kunitada Shimotohno, Masashi Mizokami, Yasuo Ariumi
Summary: The renin-angiotensin-aldosterone system plays a significant role in SARS-CoV-2 infection, with ACE1 gene polymorphisms potentially linked to the severity of COVID-19 symptoms. Variations in ACE1 insertion/deletion polymorphism may explain mortality differences between regions, while research on the ACE1 genotype could offer new insights into the pathogenesis, treatment, and diagnosis of SARS-CoV-2 infections.
Article
Genetics & Heredity
Yasuo Ariumi
Summary: Hepatitis B virus (HBV) restricts the retrotransposition of long interspersed element-1 (LINE-1, L1) in a reverse transcriptase (RT)-independent manner, with HBV polymerase (Pol) playing a key role in inhibiting L1 retrotransposition.
Article
Biochemistry & Molecular Biology
Weilin Gu, Youki Ueda, Hiromichi Dansako, Shinya Satoh, Nobuyuki Kato
Summary: This study focused on the anti-HCV activity of N-89 (N-251) and found that the acquisition of N-89 (N-251) resistance in HCV-RNA replication involved both host factors (RAC2 and CKMT1B genes) and viral factors (M414I mutation in HCV NS5B), which acted additively to increase resistance.
Article
Biochemistry & Molecular Biology
Daichi Onomura, Shinya Satoh, Youki Ueda, Hiromichi Dansako, Nobuyuki Kato
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Hiromichi Dansako, Hirotaka Imai, Youki Ueda, Shinya Satoh, Kunitada Shimotohno, Nobuyuki Kato
Article
Biochemistry & Molecular Biology
Youki Ueda, Weilin Gu, Hiromichi Dansako, Hye-Sook Kim, Sayaka Yoshizaki, Nobuaki Okumura, Tomohiro Ishikawa, Hironori Nishitsuji, Fumihiro Kato, Takayuki Hishiki, Shinya Satoh, Koji Ishii, Michiaki Masuda, Kunitada Shimotohno, Masanori Ikeda, Nobuyuki Kato
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2018)
Article
Virology
Mohamed S. H. Hassan, Ahmed Ali, Motamed Elsayed Mahmoud, Danah Altakrouni, Shahnas M. Najimudeen, Mohamed Faizal Abdul-Careem
Summary: This study compared the efficacy of different vaccination programs against Canadian DMV/1639 IBV and found that the autogenous inactivated DMV/1639 vaccine was more effective in reducing viral loads and boosting virus neutralization titers. Both vaccination programs provided significant protection against pathological effects.
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.
Article
Virology
Xiao Han, Hankun Xu, Yifan Weng, Rong Chen, Jidong Xu, Tong Cao, Renjie Sun, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: Classical swine fever virus (CSFV) can inhibit the host innate immunity by decreasing the level of high mobility group box 1 (HMGB1), and CSFV Npro can evade host antiviral immune response through upregulating HMGB1 acetylation.
Article
Virology
Sunjian Lyu, Fulei Xiong, Tianpeng Qi, Weifeng Shen, Qi Guo, Mingming Han, Li Liu, Weishao Bu, Julin Yuan, Bao Lou
Summary: This study identified and characterized a novel temperate A. hydrophila phage, P05B, which has strong lytic ability and stability across different temperatures and pH values.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Virology
Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang
Summary: Coxsackievirus A6 (CVA6) has emerged as an important agent causing hand, foot, and mouth disease (HFMD). Mutations in residue 64 of the virus have been found to increase resistance to ribavirin and decrease sensitivity to guanidine hydrochloride. This mutation also leads to increased replication fidelity and reduced pathogenicity. These findings provide a foundation for the development of a safe and effective live attenuated CVA6 vaccine.
Article
Virology
Yao Xiong, Keran Ma, Xiao Zou, Yantao Liang, Kaiyang Zheng, Tiancong Wang, Hong Zhang, Yue Dong, Ziyue Wang, Yundan Liu, Hongbing Shao, Andrew Mcminn, Min Wang
Summary: This study isolated a novel virus, vB_VviC_ZQ26, infecting Vibrio cyclitrophicus from coastal waters near Qingdao, China. It was found that vB_VviC_ZQ26 belongs to a new vibriophage-specific family, Coheviridae, and is mainly found in temperate and tropical epipelagic zones.
Article
Virology
Xing Li, Zhiping Ye, Ewan P. Plant
Summary: By analyzing clinical and cultured samples using the same bioinformatic pipeline, it was found that 5' copyback DVGs are prevalent in human clinical samples but not in cultured samples. Furthermore, there are differences in DVG production and composition between in vivo and in vitro infections.
Article
Virology
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Suvrotoa Mitra, Animesh Gope, Mamta Chawla-Sarkar
Summary: Rotavirus is a major cause of viral gastroenteritis in infants and continues to be a severe issue in underdeveloped nations. Recent research has shown that a long non-coding RNA called SLC7A11-AS1 is upregulated during rotavirus infection and plays a role in facilitating the virus's propagation by regulating intracellular glutathione and lipid peroxidation.
Article
Virology
Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
Summary: Bat-borne emerging viruses, such as the Ebola virus and Nipah virus, pose a significant threat to public health. In this study, we identified micafungin as a potential antiviral drug against Pteropine orthoreovirus (PRV) and found that it inhibits viral release in human cells through activating the endoplasmic reticulum stress response pathway. This research provides valuable insights into the development of effective treatments for future virus pandemics.
Article
Virology
M. Joubert, N. van den Berg, J. Theron, V. Swart
Summary: This study investigated the host gene expression in asymptomatic avocado nursery trees infected with Avocado sunblotch viroid (ASBVd) using RNA sequencing. The results showed that 631 genes were differentially expressed during infection, with 63% upregulated. ASBVd infection significantly affected plant defence responses, phytohormone networks, gene expression pathways, secondary metabolism, cellular transport, as well as protein modification and degradation. This study provides insights into the molecular interactions underlying avsunviroid-host interactions.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.
Article
Virology
Manel Essaidi-Laziosi, Francisco J. Perez-Rodriguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A. Mueller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
Summary: This study found that the Omicron variant has different replication characteristics compared to other variants of concern, being able to efficiently produce infectious virus in nasal cells but not lung cells. In addition, the Delta and Omicron variants showed increased infection efficiency in A549 cells. These findings suggest that the variants of concern have better adaptation to humans but do not have an extended host range.
Article
Virology
Martin Faye, Modeste Name Faye, Babacar Ndiaye, Moussa Moise Diagne, Safietou Sankhe, Ndeye Marieme Top, Amadou Diallo, Cheikh Loucoubar, Ndongo Dia, Amadou Alpha Sall, Ousmane Faye
Summary: Genomic surveillance in Senegal since March 2020 has detected the emergence of Omicron BA.4 and BA.5 sublineages in June 2022. Next-generation sequencing and phylogeny analysis of isolates from a cluster of cases in Northern Senegal in July 2022 revealed that the BA.4 cases originated from a XAS recombinant, marking the first reported sequence of this variant from Senegal. Continuous genomic surveillance of positive SARS-CoV-2 samples is crucial.
