Journal
VIRUS RESEARCH
Volume 136, Issue 1-2, Pages 140-145Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2008.05.001
Keywords
P1 function; plantibody-mediated resistance; Polerovirus
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The P1 protein of Potato leafroll virus (PLRV) is thought to play a major role in the replication cycle by promoting the maturation of the genome-linked virion protein VPg. To study the relevance of P1 and its autoproteolytic derivative Pl-C25 in the viral life cycle, the V-H and V-L domains of monoclonal antibody mAbP1-1, raised against the C-terminus of PI, were used to develop a single chain variable fragment antibody scFvP1-1 for expression in plants. The transient expression of scFvP1-1 in tobacco (Nicotiana tabacum) strongly reduced virus accumulation, while transgenic potato (Solanum tuberosum) plants expressing scFvP1-1 showed high levels of resistance following PLRV inoculation by viruliferous aphids. This is the first report that conclusively demonstrates that a PLRV gene product is essential for the completion of the virus life cycle in vivo without genetic alteration of the viral genome. This is also the first time plantibody-mediated resistance has been demonstrated with a luteovirus. (c) 2008 Elsevier B.V. All rights reserved.
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