Article
Genetics & Heredity
Xiao-Ling Su, Zhong-Yue Mai, Kun-Jiang Wei, Yang-Jian Huang, Hong-Li Shan, De-Jie Cheng
Summary: In this study, the complete genome of a sugarcane mosaic sample from southern China was sequenced and analyzed. The results showed that the sugarcane streak mosaic virus (SCSMV) is the primary pathogen of sugarcane mosaic disease in this region. The phylogenetic analysis revealed the genetic relationship between different SCSMV isolates, and the selective pressure analysis provided insights into the evolution and adaptation of the virus. These findings have practical implications for the monitoring and genetic studies of SCSMV.
Article
Plant Sciences
Nar B. Ranabhat, John P. Fellers, Myron A. Bruce, Jessica L. Shoup Rupp
Summary: Wheat breeders are using new sequencing technology to study the natural field virus populations in Kansas. They found brome mosaic virus (BMV) present in 29 out of 47 sampled counties, often co-infected with other wheat viruses. The study also revealed the genetic characteristics and variations of BMV isolates. This information is important for plant breeding and improving monitoring tools for virus detection.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Li Jiang, Qiang Zhang, Shimin Xiao, Fusheng Si
Summary: By systematically analyzing 107 strains of SMV, this study revealed the codon usage profile of the SMV genome and identified the factors influencing its codon usage patterns. The findings suggest that natural selection based on geographical diversity plays a critical role in shaping the codon usage preferences of SMV.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Virology
Mariola Usovsky, Pengyin Chen, Dexiao Li, Aiming Wang, Ainong Shi, Cuiming Zheng, Ehsan Shakiba, Dongho Lee, Caio Canella Vieira, Yi Chen Lee, Chengjun Wu, Innan Cervantez, Dekun Dong
Summary: This review summarizes the history and current state of the genetic basis for soybean resistance to Soybean mosaic virus (SMV) and the application of molecular markers in crop improvement. It discusses the molecular underpinnings of SMV-soybean interactions and the distribution and interactions of resistance genes. Although the physical position of some resistance genes has been identified, there are still elusive causative genes. Soybean resistance to SMV has been achieved through the combination of multiple genes.
Article
Plant Sciences
Kai Zhang, Yingchao Shen, Tao Wang, Yu Wang, Song Xue, Hexiang Luan, Liqun Wang, Kai Li, Dongquan Guo, Haijian Zhi
Summary: The leaves of soybean cultivar ZheA8901 exhibit different symptoms when infected with different strains of soybean mosaic virus (SMV). A proteomic analysis identified key proteins associated with mosaic and symptomless leaves, namely GmGSTU13 and ascorbate peroxidase (APX), respectively. The upregulation of GmGSTU13 was found to be correlated with SMV-induced mosaic symptoms, while the upregulation of APX was associated with symptomless leaves. This study provides valuable information for identifying key proteins involved in symptom development in soybean infected with different SMV strains.
Article
Plant Sciences
Hua Jiang, Kai Li, Junyi Gai
Summary: The study revealed that targeting the viral genome via host-induced gene silencing (HIGS) could explore broad-spectrum resistance (BSR) to Soybean mosaic virus (SMV), with S1-TIR showing the best performance in inhibiting SMV multiplication. The efficacy of S1-TIR was validated in transgenic soybean using Nicotiana benthamiana-chimeric-SMV assays, suggesting its potential as a breeding source for SMV control in field.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Kristin Widyasari, Phu-Tri Tran, Jiyoung Shin, Hokyoung Son, Kook-Hyung Kim
Summary: The purple acid phosphatase GmPAP2.1 from soybean cultivar L29 acts as a resistance factor against specific strains of Soybean mosaic virus (SMV) by up-regulating the ICS1 gene to activate the salicylic acid (SA) pathway for defense response. Overexpression of GmPAP2.1 from L29 confers SMV resistance to susceptible cultivar Lee 74 via SA pathway accumulation.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Agriculture, Multidisciplinary
Yi-ran Zhao, Yu-zhong Zhao, Si-dang Liu, Yi-hong Xiao, Ning Li, Kui-hao Liu, Fan-liang Meng, Jun Zhao, Meng-da Liu, Bao-quan Li
Summary: During surveillance in China, 11 H9N2 avian influenza viruses (AIVs) were isolated from chickens in Shandong Province. The isolates showed close genetic relationship with several sublineages of Eurasian lineage and had mutation sites associated with human receptor binding, mammalian fitness, antigenic variation, and drug resistance. Studies with mice revealed varying levels of pathogenicity among the isolates. The average antibody titer of the H9N2 AIVs was 8.60 log2. Epidemiological surveillance of H9N2 AIVs is crucial based on these findings.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Agronomy
Tianjie Sun, Xizhe Sun, Fukuan Li, Nan Ma, Mengxuan Wang, Yan Chen, Na Liu, Yuan Jin, Jie Zhang, Chunyan Hou, Chunyan Yang, Dongmei Wang
Summary: In response to Soybean mosaic virus (SMV) infection, soybean exhibits a defense mechanism by depositing callose on plasmodesmata to block virus transport. This study reveals that hydrogen peroxide (H2O2) plays a crucial role in regulating callose accumulation. Transcriptome analysis identifies two genes, GmSEOB and GmPAP27, regulated by H2O2, which positively regulate callose accumulation in response to SMV infection.
Article
Biotechnology & Applied Microbiology
Soumya Sinha, Abdul Samad
Summary: A new strain of Cucumber mosaic virus infecting Salvia sclarea was identified for the first time, causing leaf symptoms and being mechanically transmissible. Phylogenetic analysis revealed its relationship to subgroup IA of CMV, with unique changes in the MP and conserved CP.
Article
Agronomy
Yingchao Shen, Adhimoolam Karthikeyan, Yunhua Yang, Na Ma, Jinlong Yin, Yuan Yuan, Liqun Wang, Haijian Zhi
Summary: This study investigated the functional response of the soybean FNR gene during soybean mosaic virus (SMV) infection by silencing the gene in resistant and susceptible lines. The silencing of GmFNR resulted in decreased photosynthetic capacity and catalase activity in both lines, with a significant increase in H2O2 content in the susceptible line. SMV infection in the susceptible line caused serious morphological changes and increased SMV NIa-protease transcript accumulation; however, this impact was not observed in the resistant line.Analysis revealed that GmFNR interacts with EF1A and coincides with increased SMV accumulation, providing novel insights into the SMV resistance mechanism.
Article
Virology
Ali Celik, Ali Ferhan Morca, Sevgi Coskan, Adyatma Irawan Santosa
Summary: The gene sequence data of apple mosaic virus (ApMV) in NCBI GenBank were analyzed to determine its global phylogeny and population structure. The movement protein (MP) and coat protein (CP) genes showed identical phylogenies, indicating the presence of recombinant isolates. The isolates in group 3 had higher divergence among them, and there was genetic separation and lack of gene flow among the three phylogroups.
Article
Agronomy
Qiuyan Ren, Hua Jiang, Wenyang Xiang, Yang Nie, Song Xue, Haijian Zhi, Kai Li, Junyi Gai
Summary: This study cloned the resistance gene GmCAL against Soybean mosaic virus and found that it plays a crucial regulatory role in soybean resistance.
Article
Virology
Xintao Gao, Jialei Li, Tong Wu, Jinping Dou, Wenrong Zhang, Hong Jia, Zhifang Zhang, Xingjian Liu, Yinue Li
Summary: In this study, a novel strain of GETV, named BJ0304, was successfully isolated and its growth kinetics were determined. Genetic and phylogenetic analyses revealed that BJ0304 was clustered in Group III and most closely related to the GETV-V1 strain. The pathogenicity of BJ0304 was assessed and found to be less virulent in mice compared to its closest homolog GETV-V1. Mutation and glycosylation analysis identified a unique mutation (171 T > I) in the E2 protein that affected its glycosylation and may be associated with viral pathogenicity.
Article
Plant Sciences
Lin Zhao, Xin Wei, Tao Zheng, Ya-Nan Gou, Jun Wang, Jian-Xin Deng, Mei-Jia Li
Summary: This study collected soybean seeds with serious symptoms of rotted pods from a farm in Hubei Province, China. Fusarium strains were isolated from the seeds and two Fusarium species were identified. The strains from the Fusarium fujikuroi species complex showed higher pathogenicity on soybean seeds, roots, and pods compared to the strains from the Fusarium incarnatum-equiseti species complex. F. proliferatum YZU 201408 was found to be the most pathogenic strain, causing disease severity of 72.2% to 90% in all tests.
Article
Biochemistry & Molecular Biology
Maryam Hajikarimlou, Kathryn Hunt, Grace Kirby, Sarah Takallou, Sasi Kumar Jagadeesan, Katayoun Omidi, Mohsen Hooshyar, Daniel Burnside, Houman Moteshareie, Mohan Babu, Myron Smith, Martin Holcik, Bahram Samanfar, Ashkan Golshani
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Jitender Mehla, George Liechti, Randy M. Morgenstein, J. Harry Caufield, Ali Hosseinnia, Alla Gagarinova, Sadhna Phanse, Norman Goodacre, Mary Brockett, Neha Sakhawalkar, Mohan Babu, Rong Xiao, Gaetano T. Montelione, Sergey Vorobiev, Tanneke den Blaauwen, John F. Hunt, Peter Uetz
Summary: YhcB, a conserved protein across gamma-proteobacteria with unknown function, plays a crucial role in cell division and affects envelope biogenesis. Based on its interactions with key proteins in divisome and elongasome, it is proposed to be renamed as ZapG for its association with the Z-ring. This study provides insights for future research on the protein family and cell transition from exponential to stationary survival.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Correction
Multidisciplinary Sciences
Kamran Rizzolo, Angela Yeou Hsiung Yu, Adedeji Ologbenla, Sa Rang Kim, Haojie Zhu, Koichiro Ishimori, Guillaume Thibault, Elisa Leung, Yi Wen Zhang, Mona Teng, Marta Haniszewski, Noha Miah, Sadhna Phanse, Zoran Minic, Sukyeong Lee, Julio Diaz Caballero, Mohan Babu, Francis T. F. Tsai, Tomohide Saio, Walid A. Houry
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Nelson Martinez-Matias, Nataliya Chorna, Sahily Gonzalez-Crespo, Lilliam Villanueva, Ingrid Montes-Rodriguez, Loyda M. Melendez-Aponte, Abiel Roche-Lima, Kelvin Carrasquillo-Carrion, Ednalise Santiago-Cartagena, Brian C. Rymond, Mohan Babu, Igor Stagljar, Jose R. Rodriguez-Medina
Summary: Functional analysis of the Mtl1 protein in Saccharomyces cerevisiae shows that it enhances yeast cells' resistance to oxidative stress through the cell wall integrity pathway. The protein also plays a role in regulating non-lethal heat stress response and metabolic regulatory mechanisms. Proteomic and metabolomic analysis indicates altered levels of metabolites, enzyme expression, and mitochondrial proteins in mtl1 Delta strains.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Matthew Jessulat, Shahreen Amin, Mohsen Hooshyar, Ramy Malty, Mohamed Taha Moutaoufik, Mara Zilocchi, Zoe Istace, Sadhna Phanse, Hiroyuki Aoki, Katayoun Omidi, Daniel Burnside, Bahram Samanfar, Khaled A. Aly, Ashkan Golshani, Mohan Babu
Summary: The deletion of the yeast PKA catalytic subunit Tpk1 reduces NHEJ efficiency by phosphorylating Nej1 to influence repair protein recruitment and DNA resection. In mammalian cells, the PRKACB homolog also plays a similar role in NHEJ repair by phosphorylating XLF.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Thiago Seraphim, Nardin Nano, Yiu Wing Sunny Cheung, Siripat Aluksanasuwan, Carolina Colleti, Yu-Qian Mao, Vaibhav Bhandari, Gavin Young, Larissa Holl, Sadhna Phanse, Yuliya Gordiyenko, Daniel R. Southworth, Carol Robinson, Visith Thongboonkerd, Lisandra M. Gava, Julio C. Borges, Mohan Babu, Leandro R. S. Barbosa, Carlos H. Ramos, Philipp Kukura, Walid A. Houry
Summary: The R2TP complex, formed by RUVBL1 and RUVBL2 ATPases, associates with PIH1D1 and RPAP3 proteins and plays a role in promoting the formation of macromolecular complexes. RPAP3 is identified as the central subunit of R2TP, linking PIH1D1 and RUVBL1/2. The study provides insights into the structure and function of the R2TP complex.
Review
Genetics & Heredity
Matineh Rahmatbakhsh, Alla Gagarinova, Mohan Babu
Summary: Microbial pathogens have evolved diverse strategies to manipulate host systems, causing diseases through changes in the host-pathogen proteome. Mass spectrometry-based proteomics approaches are utilized to study disease progression but present challenges in data analysis. The study reviews steps in temporal and spatial analysis, offering best practices for data preprocessing, statistical analysis, and biological information extraction. Guidance is provided for novices and established users, with future directions and data analysis codes available for testing.
FRONTIERS IN GENETICS
(2021)
Article
Multidisciplinary Sciences
Mara Zilocchi, Cheng Wang, Mohan Babu, Jingjing Li
Summary: The article discusses the qualitative and quantitative definitions of health, as well as understanding the molecular etiologies of diseases through a proteomic perspective. It highlights key proteomic approaches to identify pathogenic mutations and integrating omics data to define the borderline between health and disease.
Article
Biochemical Research Methods
Kevin Dick, Arezo Pattang, Julia Hooker, Nour Nissan, Michael Sadowski, Bradley Barnes, Le Hoa Tan, Daniel Burnside, Sadhna Phanse, Hiroyuki Aoki, Mohan Babu, Frank Dehne, Ashkan Golshani, Elroy R. Cober, James R. Green, Bahram Samanfar
Summary: By analyzing the protein-protein interaction network between humans and soybeans, potential important proteins related to human health and putative allergens can be identified. The study leverages advanced PPI predictors to predict interactions between humans and soybeans, exploring proteins related to immune and neurological pathways.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Sang Hyun Lim, Jamie Snider, Liron Birimberg-Schwartz, Wan Ip, Joana C. Serralha, Hugo M. Botelho, Miqueias Lopes-Pacheco, Madalena C. Pinto, Mohamed Taha Moutaoufik, Mara Zilocchi, Onofrio Laselva, Mohsen Esmaeili, Max Kotlyar, Anna Lyakisheva, Priscilla Tang, Lucia Lopez Vazquez, Indira Akula, Farzaneh Aboualizadeh, Victoria Wong, Ingrid Grozavu, Teuta Opacak-Bernardi, Zhong Yao, Meg Mendoza, Mohan Babu, Igor Jurisica, Tanja Gonska, Christine E. Bear, Margarida D. Amaral, Igor Stagljar
Summary: Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is a chloride and bicarbonate channel that plays a critical role in maintaining fluid homeostasis. Mutations in CFTR are associated with Cystic Fibrosis (CF), the most common lethal autosomal recessive disorder in Caucasians. This study used a high-throughput screening technique to map the protein-protein interactions of CFTR and identify potential drug targets for CF treatment. They discovered candidate proteins, such as FGL2, that may have significant effects on CFTR function in CF pathophysiology.
MOLECULAR SYSTEMS BIOLOGY
(2022)
Article
Virology
Subha Dahal, Ran Cheng, Peter K. Cheung, Terek Been, Ramy Malty, Melissa Geng, Sarah Manianis, Lulzim Shkreta, Shahrazad Jahanshahi, Johanne Toutant, Rose Chan, Sean Park, Mark A. Brockman, Mohan Babu, Samira Mubareka, Karen Mossman, Arinjay Banerjee, Scott Gray-Owen, Martha Brown, Walid A. Houry, Benoit Chabot, David Grierson, Alan Cochrane
Summary: In this study, the medicinal chemistry optimization of a known HIV-1 inhibitor led to the discovery of a new derivative, GPS491, which showed potent anti-HIV-1 activity with reduced toxicity. The study demonstrated that GPS491 not only blocked HIV-1 replication, but also inhibited the replication of adenovirus and multiple coronaviruses. Furthermore, GPS491 induced changes in viral RNA processing and altered the accumulation/phosphorylation/function of splicing regulatory SR proteins. This study highlights the potential of targeting cellular factors involved in RNA processing for the development of broad-spectrum antiviral agents.
Article
Neurosciences
Elisabet Jakova, Mohamed Taha Moutaoufik, Jeremy S. Lee, Mohan Babu, Francisco S. Cayabyab
Summary: This study found that adenosine and A1R receptor ligands interact with alpha-syn to modulate its aggregation and neurodegeneration. A1R agonists and drugs promoting a knot conformation of alpha-syn can cause alpha-synucleinopathy and increase neuronal degeneration, whereas A1R antagonists and drugs promoting a loop conformation of alpha-syn can be harnessed for possible neuroprotective therapies.
TRANSLATIONAL NEURODEGENERATION
(2022)
Article
Multidisciplinary Sciences
Alla Gagarinova, Ali Hosseinnia, Matineh Rahmatbakhsh, Zoe Istace, Sadhna Phanse, Mohamed Taha Moutaoufik, Mara Zilocchi, Qingzhou Zhang, Hiroyuki Aoki, Matthew Jessulat, Sunyoung Kim, Khaled A. Aly, Mohan Babu
Summary: By studying genetic interactions among all transcription factor genes in E. coli, this research reveals condition-dependent interactions and uncovers the roles of uncharacterized transcription factors.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Khaled A. Aly, Mohamed Taha Moutaoufik, Mara Zilocchi, Sadhna Phanse, Mohan Babu
Summary: Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is a rare early-onset neurodegenerative disease caused by mutations in the SACS gene. Understanding the function of Sacsin and using ARSACS disease models can improve our knowledge of this disease. Gene correction strategies and delivery methods provide hope for the development of therapeutics for ARSACS.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Premkumari Kumarathasan, Nazila Nazemof, Dalibor Breznan, Erica Blais, Hiroyuki Aoki, James Gomes, Renaud Vincent, Sadhna Phanse, Mohan Babu
Summary: This study evaluated the internalization and mitochondrial effects of silica nanoparticles (SiNPs) with different sizes and surface modifications. The findings suggest that physicochemical properties are determinants of SiNP exposure-related mitochondrial effects. Mitochondrial exposures combined with proteomic analysis can serve as a new approach in risk assessment, providing insights into related toxicity mechanisms.
Article
Virology
Mohamed S. H. Hassan, Ahmed Ali, Motamed Elsayed Mahmoud, Danah Altakrouni, Shahnas M. Najimudeen, Mohamed Faizal Abdul-Careem
Summary: This study compared the efficacy of different vaccination programs against Canadian DMV/1639 IBV and found that the autogenous inactivated DMV/1639 vaccine was more effective in reducing viral loads and boosting virus neutralization titers. Both vaccination programs provided significant protection against pathological effects.
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.
Article
Virology
Xiao Han, Hankun Xu, Yifan Weng, Rong Chen, Jidong Xu, Tong Cao, Renjie Sun, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: Classical swine fever virus (CSFV) can inhibit the host innate immunity by decreasing the level of high mobility group box 1 (HMGB1), and CSFV Npro can evade host antiviral immune response through upregulating HMGB1 acetylation.
Article
Virology
Sunjian Lyu, Fulei Xiong, Tianpeng Qi, Weifeng Shen, Qi Guo, Mingming Han, Li Liu, Weishao Bu, Julin Yuan, Bao Lou
Summary: This study identified and characterized a novel temperate A. hydrophila phage, P05B, which has strong lytic ability and stability across different temperatures and pH values.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Virology
Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang
Summary: Coxsackievirus A6 (CVA6) has emerged as an important agent causing hand, foot, and mouth disease (HFMD). Mutations in residue 64 of the virus have been found to increase resistance to ribavirin and decrease sensitivity to guanidine hydrochloride. This mutation also leads to increased replication fidelity and reduced pathogenicity. These findings provide a foundation for the development of a safe and effective live attenuated CVA6 vaccine.
Article
Virology
Yao Xiong, Keran Ma, Xiao Zou, Yantao Liang, Kaiyang Zheng, Tiancong Wang, Hong Zhang, Yue Dong, Ziyue Wang, Yundan Liu, Hongbing Shao, Andrew Mcminn, Min Wang
Summary: This study isolated a novel virus, vB_VviC_ZQ26, infecting Vibrio cyclitrophicus from coastal waters near Qingdao, China. It was found that vB_VviC_ZQ26 belongs to a new vibriophage-specific family, Coheviridae, and is mainly found in temperate and tropical epipelagic zones.
Article
Virology
Xing Li, Zhiping Ye, Ewan P. Plant
Summary: By analyzing clinical and cultured samples using the same bioinformatic pipeline, it was found that 5' copyback DVGs are prevalent in human clinical samples but not in cultured samples. Furthermore, there are differences in DVG production and composition between in vivo and in vitro infections.
Article
Virology
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Suvrotoa Mitra, Animesh Gope, Mamta Chawla-Sarkar
Summary: Rotavirus is a major cause of viral gastroenteritis in infants and continues to be a severe issue in underdeveloped nations. Recent research has shown that a long non-coding RNA called SLC7A11-AS1 is upregulated during rotavirus infection and plays a role in facilitating the virus's propagation by regulating intracellular glutathione and lipid peroxidation.
Article
Virology
Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
Summary: Bat-borne emerging viruses, such as the Ebola virus and Nipah virus, pose a significant threat to public health. In this study, we identified micafungin as a potential antiviral drug against Pteropine orthoreovirus (PRV) and found that it inhibits viral release in human cells through activating the endoplasmic reticulum stress response pathway. This research provides valuable insights into the development of effective treatments for future virus pandemics.
Article
Virology
M. Joubert, N. van den Berg, J. Theron, V. Swart
Summary: This study investigated the host gene expression in asymptomatic avocado nursery trees infected with Avocado sunblotch viroid (ASBVd) using RNA sequencing. The results showed that 631 genes were differentially expressed during infection, with 63% upregulated. ASBVd infection significantly affected plant defence responses, phytohormone networks, gene expression pathways, secondary metabolism, cellular transport, as well as protein modification and degradation. This study provides insights into the molecular interactions underlying avsunviroid-host interactions.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.
Article
Virology
Manel Essaidi-Laziosi, Francisco J. Perez-Rodriguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A. Mueller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
Summary: This study found that the Omicron variant has different replication characteristics compared to other variants of concern, being able to efficiently produce infectious virus in nasal cells but not lung cells. In addition, the Delta and Omicron variants showed increased infection efficiency in A549 cells. These findings suggest that the variants of concern have better adaptation to humans but do not have an extended host range.
Article
Virology
Martin Faye, Modeste Name Faye, Babacar Ndiaye, Moussa Moise Diagne, Safietou Sankhe, Ndeye Marieme Top, Amadou Diallo, Cheikh Loucoubar, Ndongo Dia, Amadou Alpha Sall, Ousmane Faye
Summary: Genomic surveillance in Senegal since March 2020 has detected the emergence of Omicron BA.4 and BA.5 sublineages in June 2022. Next-generation sequencing and phylogeny analysis of isolates from a cluster of cases in Northern Senegal in July 2022 revealed that the BA.4 cases originated from a XAS recombinant, marking the first reported sequence of this variant from Senegal. Continuous genomic surveillance of positive SARS-CoV-2 samples is crucial.
