Article
Plant Sciences
Madhu Tiwari, Neelam Gautam, Yuvraj Indoliya, Maria Kidwai, Arun Kumar Mishra, Debasis Chakrabarty
Summary: This study identified OsGSTU5 as a cellular interactor of VirE2 in rice and established its important role in modulating Agrobacterium-mediated transformation efficiency in rice.
PLANT CELL REPORTS
(2022)
Review
Microbiology
Karuppannan Veluthambi, Sukumaran Sunitha
Summary: Geminiviruses are a group of plant DNA viruses that cause significant losses to economically important crops, mainly by infecting hosts through the suppression of host-mediated defense mechanisms. Research has extensively investigated the mechanism of action of the major viral suppressor AC2/AL2/TrAP.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Rachelle A. Lapham, Lan-Ying Lee, Eder Xhako, Esteban Ganan Gomez, V. M. Nivya, Stanton B. Gelvin
Summary: VirE2, an effector protein of Agrobacterium, plays a crucial role in plant transformation by coating and protecting transferred DNA in the plant cytoplasm. Plant-expressed VirE2 can complement a virE2 mutant strain, but nuclear-localized VirE2 cannot stimulate transformation. VirE2 modulates plant RNA and protein levels to facilitate transformation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Horticulture
Hiroto Yamamoto, Yuya Wakita, Toshiya Kitaoka, Kohei Fujishiro, Elly Kesumawati, Sota Koeda
Summary: The tomato leaf curl New Delhi virus (ToLCNDV) poses a serious threat to horticultural crops in various regions. This study isolated full-length ToLCNDV viral sequences from plants in Spain, conducted phylogenetic analysis and agroinoculation experiments, revealing differences in disease symptoms severity among different strains and plant species.
HORTICULTURE JOURNAL
(2021)
Article
Plant Sciences
Kenji Kubota, Yasuhiro Tomitaka, Tomio Usugi, Hiroyuki Hamada, Hiroki Ito, Atsushi Kuwana, Shinya Tsuda
Summary: A new geminivirus, named tomato curly top virus, was discovered in field-grown tomatoes in Fukushima, Japan. It showed a close relationship to common bean curly stunt virus, with high sequence identity.
JOURNAL OF GENERAL PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Yen-Wen Kuo, Alicja Bednarska, Maher Al Rwahnih, Bryce W. Falk
Summary: Developed an improved injection method for delivering viral infectious clones into greenhouse-grown grapevine plants. Developed infectious clones for two isolates of grapevine geminivirus A and found that they accumulated to different viral titers in different parts of the infected grapevine plants. These tools can be applied to grapevine plants, potentially for disease management and trait improvements.
Article
Plant Sciences
Giovana Acha, Ricardo Vergara, Marisol Munoz, Roxana Mora, Carlos Aguirre, Manuel Munoz, Julio Kalazich, Humberto Prieto
Summary: In this study, a Bean yellow dwarf virus-derived replicon vector was successfully utilized for gene editing in potato, showing potential for targeted gene deletion and single site edits. The results indicate promising outcomes for gene editing in potato and provide insights into repair alternatives for targeted genes.
Article
Biotechnology & Applied Microbiology
Ying Li, Min Sun, Xin Wang, Yue-Jing Zhang, Xiao-Wei Da, Ling-Yun Jia, Hai-Long Pang, Han-Qing Feng
Summary: The appropriate use of alpha-naphthalene acetic acid, gibberellins(3), and 6-benzyladenine can enhance plant biomass and transient expression efficiency, with alpha-naphthalene acetic acid and gibberellins(3) beneficial for improving the expression level of green fluorescent protein.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Biology
Tomoaki Mori, Kosuke Takenaka, Fumiya Domoto, Yasuhiro Aoyama, Takashi Sera
Summary: The study successfully established a quick method to evaluate TYLCV infection using cotyledons of Micro-Tom, a miniature tomato cultivar. By constructing a binary plasmid harboring TYLCV genome copies, the susceptibility of Micro-Tom to TYLCV was confirmed. By agroinoculating cotyledons of Micro-Tom 10 days after sowing seeds, typical symptoms of TYLCV infection were consistently observed on true leaves 10 days after inoculation. Molecular analysis detected TYLCV progeny DNA in all leaves demonstrating symptoms 6 days after inoculation, enabling assessment of TYLCV infection within 20 days after sowing seeds.
BMC RESEARCH NOTES
(2021)
Article
Biotechnology & Applied Microbiology
Sukumaran Sunitha, Nagrani Mahajan, Karuppannan Veluthambi
PLANT CELL TISSUE AND ORGAN CULTURE
(2012)
Article
Plant Sciences
Doron Teper, Dor Salomon, Sukumaran Sunitha, Jung-Gun Kim, Mary Beth Mudgett, Guido Sessa
Article
Biochemical Research Methods
Sukumaran Sunitha, Padubidri V. Shivaprasad, Kumari Sujata, Karuppannan Veluthambi
PLANT MOLECULAR BIOLOGY REPORTER
(2012)
Article
Genetics & Heredity
Sukumaran Sunitha, Gnanasekaran Shanmugapriya, Veluthambi Balamani, Karuppannan Veluthambi
Article
Biochemical Research Methods
Sukumaran Sunitha, Christopher D. Rock
TRANSGENIC RESEARCH
(2020)
Article
Multidisciplinary Sciences
Heshani De Silva Weligodage, Gan Jin, Maninderjeet Kaur, Sukumaran Sunitha
Summary: Grapevine red blotch virus (GRBV) is a major threat to the US grapevine industry since its identification in 2011, causing significant economic impacts in the Pacific Northwest grape-growing regions. This study characterized two ORFs of GRBV, C2 and V2, as viral silencing suppressors, showing synergism or additive effects at the mRNA level when expressed together in Nicotiana benthamiana line 16c GFP marker plants. Furthermore, no self-interaction or physical interaction between C2 and V2 proteins was observed.
Article
Biochemistry & Molecular Biology
Doron Teper, Sukumaran Sunitha, Gregory B. Martin, Guido Sessa
PLANT SIGNALING & BEHAVIOR
(2015)
Article
Biochemistry & Molecular Biology
Rajendran Rajeswaran, Sukumaran Sunitha, Padubidri V. Shivaprasad, Mikhail M. Pooggin, Thomas Hohn, Karuppannan Veluthambi
MOLECULAR PLANT-MICROBE INTERACTIONS
(2007)