Article
Microbiology
Yifeng Qin, Shuying Qin, Xiangmei Huang, Lishi Xu, Kang Ouyang, Ying Chen, Zuzhang Wei, Weijian Huang
Summary: In this study, two field strains of pseudorabies virus (PRV) were isolated and characterized. The GXLB-2015 strain showed genetic recombination between a foreign PRV strain and Chinese PRV variants, while the GXGG-2016 strain had a natural deletion in the thymidine kinase (TK) gene. Pathogenicity analysis revealed that GXLB-2015 strain was highly pathogenic to mice, while the GXGG-2016 strain was not pathogenic. These findings contribute to our understanding of PRV evolution in China and have implications for the control of PRV infection in pig farms.
VETERINARY MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Nanhua Chen, Xinshuai Li, Yanzhao Xiao, Shubin Li, Jianzhong Zhu
Summary: Research has shown that PRRS viruses derived from MLVs are circulating and evolving in Chinese swine herds for years, emphasizing the necessity for the fair use of PRRS MLVs.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Chang-Zhan Xie, Yi-Mo Tao, Zhuo Ha, Ping Zhang, Ying Zhang, He Zhang, Ning-Yi Jin, Hui-Jun Lu
Summary: This study provides valuable evidence for understanding the genomic characteristics and potential pathogenicity of the NADC34-like strain 2020-Acheng-1.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Microbiology
I. Ruedas-Torres, I. M. Rodriguez-Gomez, J. M. Sanchez-Carvajal, F. Larenas-Munoz, F. J. Pallares, L. Carrasco, J. Gomez-Laguna
Summary: PRRSV is considered one of the most economically important diseases in the global pig industry, causing reproductive failure in sows and respiratory problems in growing pigs. Strains capable of producing higher mortality rates and greater severity in clinical signs and lesions have been identified since the late 1980s in the United States and Canada. However, there is still a lack of homogeneity and consensus in describing these virulent strains.
VETERINARY MICROBIOLOGY
(2021)
Article
Virology
Jiazeng Chen, Lingxue Yu, Yanjun Zhou, Shen Yang, Yun Bai, Qian Wang, Jinmei Peng, Tongqing An, Fei Gao, Liwei Li, Chao Ye, Changlong Liu, Guangzhi Tong, Xuehui Cai, Zhijun Tian, Yifeng Jiang
Summary: This study investigates the mechanisms of attenuation of highly pathogenic PRRSV and identifies the key gene and amino acids that regulate the virus's infection efficiency. The findings suggest that these factors not only affect the virus's ability to infect cells in vitro, but also impact its virulence.
Article
Immunology
Chang-zhan Xie, Zheng Wang, Zhuo Ha, Ying Zhang, Yu-biao Xie, He Zhang, Fu-long Nan, Jin-yong Zhang, Guan-yu Zhao, Zhuo-xin Li, Cheng-hui Li, Cheng-dong Yu, Ping Zhang, Hui-jun Lu, Ning-yi Jin
Summary: The study identified a new PRRSV strain, PRRSV-HB-16-China-2019, in China, which is a novel NSP2-deletion NADC30-like strain with specific deletions and mutations. This strain, recombined between a NADC30-like strain and a vaccine strain, poses a challenge to PRRSV prevention in China.
MICROBIAL PATHOGENESIS
(2021)
Article
Immunology
Xiaojuan Wang, Xiaofei Bai, Yuzhou Wang, Lulu Wang, Lulu Wei, Feifei Tan, Zhi Zhou, Kegong Tian
Summary: PRRSV-1 has caused more clinical infections in Chinese swine herds and its pathogenicity in China remains unclear. In this study, a PRRSV-1 strain, 181187-2, was isolated from a farm with abortion reports in China. The results showed that PRRSV-1 181187-2 exhibited moderate pathogenicity in piglets.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Wan-Ting Yang, I-Ju Chiu, Yao-Ting Huang, Po-Yu Liu
Summary: This study used comparative genomics to investigate the fluoroquinolone-resistant mechanisms of carbapenem-resistant E. coli (CREc) and identified a new carbapenem resistance mechanism. Additionally, the study examined the clinical characteristics of CREc and found it to be polyclonal and highly diverse in Taiwan.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Yifeng Jiang, Wu Tong, Lingxue Yu, Liwei Li, Fei Gao, Guoxin Li, Changlong Liu, Pengfei Chen, Qi Shen, Yujiao Zhang, Yanjun Zhou, Guangzhi Tong
Summary: In this study, we generated chimeric viruses to identify the virulence associated regions of HP-PRRSV during in vitro attenuation. Our results revealed that ORF1a played a crucial role in virus replication, cytokine response, and lung damage. Furthermore, the exchange of ORF1b and ORF2-7 between different backbones led to different outcomes in virus replication and cytokine response. Consistent amino acid changes were also found during PRRSV attenuation. These findings provide fundamental data for understanding PRRSV attenuation and suggest different mechanisms for virulence enhancement in vivo and attenuation in vitro.
Article
Plant Sciences
Wanxin Han, Jinhui Wang, Minna Pirhonen, Yang Pan, Jingxin Qin, Shangqing Zhang, Jiehua Zhu, Zhihui Yang
Summary: Blackleg and aerial stem rot of potato caused by soft rot enterobacteria have increased in Hebei Province, China. In this study, bacterial pathogens were identified and characterized in potato plants showing blackleg or aerial stem rot. Pectobacterium atrosepticum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Pectobacterium parmentieri were identified, along with a new species of Pectobacterium polonicum. Isolate BY21311 of P. polonicum showed reduced ability to macerate potato tubers compared to other Pectobacterium species.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Immunology
Ines Ruedas-Torres, Jaime Gomez-Laguna, Jose Maria Sanchez-Carvajal, Fernanda Larenas-Munoz, Inmaculada Barranco, Francisco Jose Pallares, Librado Carrasco, Irene Magdalena Rodriguez-Gomez
Summary: This study aimed to evaluate the expressions of TFs and cytokines during early infection with different virulent PRRSV-1 strains. The results showed upregulation of TFs T-bet, EOMES, and FOXP3, along with an increase in IFN-gamma, suggesting a stronger polarization to Th1 cells and Tregs in virulent PRRSV-1-infected animals, warranting further studies on their biological functionality.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Xing Ji, Henrike Kruger, Jin Tao, Yaxin Wang, Andrea T. Fessler, Rina Bai, Shaolin Wang, Yanjun Dong, Jianzhong Shen, Yang Wang, Stefan Schwarz, Congming Wu
Summary: Research has shown that MRSA ST9 strains isolated from pigs in China and Germany have evolved independently, while MRSA ST398 strains in these two countries have close evolutionary relationships. Factors contributing to the success of dominant clones like ST9 in China and ST398 in Germany include resistance to common antimicrobial agents, enhanced biofilm formation ability, and resistance to desiccation. Additionally, the presence of the vwb (nu Sa alpha) gene on the genome island may partly explain their colonization fitness in pigs.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Veterinary Sciences
Jiankui Liu, Liling Lai, Ye Xu, Yuan Yang, Jiarui Li, Chen Liu, Cuiqin Hunag, Chunhua Wei
Summary: Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen in the Chinese swine industry. This study analyzed the genetic sequences of four PRRSV isolates and found evidence of recombination and variations in homology between different strains. These findings are important for understanding the transmission and evolution of PRRSV.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Yanxia Zhu, Yanfen Huang, Kunhua Wei, Junnan Yu, Jianping Jiang
Summary: This study provides transcriptomic resources for Chinese Dao-di herb Zanthoxylum nitidum through SMRT sequencing technology. A total of 16,163 transcripts were obtained, with 88% successfully annotated. Additionally, 6,255 long non-coding RNAs and 22,780 simple sequence repeats were identified. Out of 100 randomly selected primer pairs, 36 were verified as positive, with 20 showing polymorphism. These findings enrich the genetic resources of Z. nitidum, facilitating future research on relevant topics.
Article
Microbiology
Ines Ruedas-Torres, Jose Maria Sanchez-Carvajal, Librado Carrasco, Francisco Jose Pallares, Fernanda Larenas-Munoz, Irene Magdalena Rodriguez-Gomez, Jaime Gomez-Laguna
Summary: This study evaluated the expression of immune checkpoints in the lungs and tracheobronchial lymph nodes of piglets infected with two different virulent strains of PRRSV-1 during the early stage of infection. The results showed that PRRSV infection dysregulates the immune response and leads to changes in the expression of costimulatory and coinhibitory molecules. The simultaneous expression of coinhibitory immune checkpoints may work together to control and limit inflammation-induced tissue damage.
FRONTIERS IN MICROBIOLOGY
(2023)
