Article
Medicine, General & Internal
Rasmus Eio Callesen, Cecilie Mullerup Kiel, Lisette Hvid Hovgaard, Kathrine Kronberg Jakobsen, Michael Papesch, Christian von Buchwald, Tobias Todsen
Summary: This study reveals that the optimal insertion depths for nasal mid-turbinate swabs are underestimated in current guidelines, providing clinical guidance for anatomically correct nasal and nasopharyngeal swab specimen collection for virus testing.
Article
Medical Laboratory Technology
Miriam B. Vos, Mark D. Gonzalez, Cheryl Stone, Rebecca Cleeton, Janet Figueroa, Robert Jerris, Sunita Park, Stacy Heilman, Risha Nayee, Ann Chahroudi, Nils Schoof, Maud Mavigner, Claudia R. Morris, Traci Leong, Amanda Grindle, Adrianna Westbrook, Wilbur Lam, Beverly B. Rogers
Summary: This study compared three sample types for SARS-CoV-2 PCR testing in children and found that saliva and mid-turbinate samples had over 95% agreement with NP swab samples when obtained within 10 days of symptom onset. However, the agreement of saliva and mid-turbinate samples in children with symptom onset >10 days prior, or without symptoms, was 82% compared to NP swab samples. Ct values from mid-turbinate samples correlated more closely with NP samples than saliva samples.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2022)
Article
Medical Laboratory Technology
Miriam B. Vos, Mark D. Gonzalez, Cheryl Stone, Rebecca Cleeton, Janet Figueroa, Robert Jerris, Sunita I. Park, Stacy Heilman, Risha Nayee, Ann Chahroudi, Nils Schoof, Maud Mavigner, Claudia R. Morris, Traci Leong, Amanda Grindle, Adrianna Westbrook, Wilbur Lam, Beverly B. Rogers
Summary: This study compares the performance of three sample types for SARS-CoV-2 testing in children. Saliva and mid-turbinate swabs collected within 10 days of symptom onset show a positive agreement of over 95% with nasopharyngeal swabs. The positive agreement for saliva and mid-turbinate samples collected from children with symptom onset over 10 days ago or without symptoms is 82% compared to nasopharyngeal swabs. Ct values from mid-turbinate nasal samples have a closer correlation with Ct values from nasopharyngeal samples than saliva samples.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2022)
Article
Multidisciplinary Sciences
Sonsoles Salto-Alejandre, Judith Berastegui-Cabrera, Pedro Camacho-Martinez, Carmen Infante-Dominguez, Marta Carretero-Ledesma, Juan Carlos Crespo-Rivas, Eduardo Marquez, Jose Manuel Lomas, Claudio Bueno, Rosario Amaya, Jose Antonio Lepe, Jose Miguel Cisneros, Jeronimo Pachon, Elisa Cordero, Javier Sanchez-Cespedes
Summary: The study assessed the ability of nasopharyngeal SARS-CoV-2 viral load at first patient's hospital evaluation to predict unfavorable outcomes in COVID-19 patients. High viral loads were associated with unfavorable outcomes but were not independently predictive. Five other predictors were independently associated with increased odds of ICU admission and death.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Nick Vereecke, Sophia Zwickl, Sophie Gumbert, Annika Graaf, Timm Harder, Mathias Ritzmann, Kathrin Lillie-Jaschniski, Sebastiaan Theuns, Julia Stadler
Summary: To date, there is a lack of comprehensive diagnostic methods to study polymicrobial infections associated with porcine respiratory disease. However, a new nanopore-based genomic diagnostic platform provides a panoramic view of viral and bacterial profiles, assisting in effective preventive and therapeutic measures.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Annalucia Biancofiore, Antonio Mirijello, Maria A. Puteo, Maria P. Di Viesti, Maria Labonia, Massimiliano Copetti, Salvatore De Cosmo, Renato Lombardi
Summary: This study evaluated the effect of Remdesivir on viral load in COVID-19 patients. The results showed that patients treated with Remdesivir had a significant reduction in viral load compared to the control group.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Agnieszka Smolinska, David S. Jessop, Kirk L. Pappan, Alexandra De Saedeleer, Amerjit Kang, Alexandra L. Martin, Max Allsworth, Charlotte Tyson, Martine P. Bos, Matt Clancy, Mike Morel, Tony Cooke, Tom Dymond, Claire Harris, Jacqui Galloway, Paul Bresser, Nynke Dijkstra, Viresh Jagesar, Paul H. M. Savelkoul, Erik V. H. Beuken, Wesley H. V. Nix, Renaud Louis, Muriel Delvaux, Doriane Calmes, Benoit Ernst, Simona Pollini, Anna Peired, Julien Guiot, Sara Tomassetti, Andries E. Budding, Frank McCaughan, Stefan J. Marciniak, Marc P. van der Schee
Summary: The study reveals that face mask filters have lower sensitivity in detecting SARS-CoV-2 and are not suitable to replace nasopharyngeal swabs for COVID-19 diagnosis. The number of viral particles collected on face mask filters is generally below the limit of detection.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, General & Internal
Ho-Jae Lim, Young-Hyun Baek, Min-Young Park, Jae-Hyun Yang, Min-Jin Kim, Nackmoon Sung, Yong-Hak Sohn, Sun-Hwa Lee, Jung-Eun Park, Yong-Jin Yang
Summary: In this study, the virus detection rate of self-collected swabs was compared to HCW-collected swabs. The results showed that the viral load in HCW-collected swabs was slightly higher than that in self-collected swabs. However, self-collection showed comparable sensitivity and specificity to HCW-collection, indicating that it can be a reliable alternative.
Article
Infectious Diseases
Olga Nikolai, Chiara Rohardt, Frank Tobian, Andrea Junge, Victor M. Corman, Terry C. Jones, Mary Gaeddert, Federica Lainati, Jilian A. Sacks, Joachim Seybold, Frank P. Mockenhaupt, Claudia M. Denkinger, Andreas K. Lindner
Summary: This study found that professional anterior nasal and nasal mid-turbinate sampling are equally accurate in ambulatory symptomatic adults, and self nasal mid-turbinate sampling is feasible and well accepted by participants.
INFECTIOUS DISEASES
(2021)
Article
Environmental Sciences
Chunhan Jin, Bin Wang, Jian Liu
Summary: The study indicates that the intensification of the Pacific quasi-decadal oscillation since the 1950s is mainly due to an increase in multi-year La Nina events, rather than the influence of the solar activity cycle.
ENVIRONMENTAL RESEARCH LETTERS
(2022)
Article
Microbiology
Emily S. Savela, Alexander Viloria Winnett, Anna E. Romano, Michael K. Porter, Natasha Shelby, Reid Akana, Jenny Ji, Matthew M. Cooper, Noah W. Schlenker, Jessica A. Reyes, Alyssa M. Carter, Jacob T. Barlow, Colten Tognazzini, Matthew Feaster, Ying-Ying Goh, Rustem F. Ismagilov
Summary: Early detection is crucial in controlling the spread of SARS-CoV-2. This study found that high-analytical-sensitivity saliva testing could detect infection several days earlier than low-analytical-sensitivity nasal-swab testing. Although nasal swabs had higher peak viral loads, they were undetectable or at lower loads during the initial days of infection. These findings emphasize the importance of acquiring early viral-load profiles to guide testing strategies and response to emerging variants.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Emergency Medicine
Pierrick Le Borgne, Morgane Solis, Francois Severac, Hamid Merdji, Yvon Ruch, Karine Alame Intern, Eric Bayle, Yves Hansmann, Pascal Bilbault, Samira Fafi-Kremer, Ferhat Meziani
Summary: The COVID-19 pandemic has had devastating effects on global healthcare systems. However, measuring viral load on nasopharyngeal swab specimens does not predict disease severity or mortality in COVID-19 patients.
ACADEMIC EMERGENCY MEDICINE
(2021)
Article
Immunology
Mazharul Maishan, Aartik Sarma, Lauren F. Chun, Saharai Caldera, Xiaohui Fang, Jason Abbott, Stephanie A. Christenson, Charles R. Langelier, Carolyn S. Calfee, Jeffrey E. Gotts, Michael A. Matthay
Summary: E-cigarette use has been increasing rapidly, but its impact on acute lung injury and acute respiratory distress syndrome caused by viral pneumonia is still unclear. Studies on mice have shown that nicotine-containing e-cigarette aerosols can suppress the immune system, exacerbate inflammation, and impair viral infection clearance, leading to worsened lung injury. These findings have important implications for the regulation of e-cigarette products.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Massimo Zollo, Veronica Ferrucci, Barbara Izzo, Fabrizio Quarantelli, Carmela Di Domenico, Marika Comegna, Carmela Paolillo, Felice Amato, Roberto Siciliano, Giuseppe Castaldo, Ettore Capoluongo
Summary: The study compared five CE-IVD kits in detecting synthetic SARS-CoV-2 viral constructs and targets in samples from twenty COVID-19-positive patients. The results showed that the kits exhibited variable performances in detecting viral target genes, with the detection of the sgN transcript being associated with higher viral loads, representing a new marker for early and more severe infection.
Article
Microbiology
Alexander Viloria Winnett, Michael K. Porter, Anna E. Romano, Emily S. Savela, Reid Akana, Natasha Shelby, Jessica A. Reyes, Noah W. Schlenker, Matthew M. Cooper, Alyssa M. Carter, Jenny Ji, Jacob T. Barlow, Colten Tognazzini, Matthew Feaster, Ying-Ying Goh, Rustem F. Ismagilov
Summary: Collecting specimens in the morning can yield higher viral loads for SARS-CoV-2 detection, improving the diagnostic sensitivity of COVID-19 testing.
MICROBIOLOGY SPECTRUM
(2022)