Review
Immunology
Kalani Ratnasiri, Aaron J. Wilk, Madeline J. Lee, Purvesh Khatri, Catherine A. Blish
Summary: This article reviews the advancements in single-cell RNA sequencing (scRNA-seq) technology and its applications in antiviral immunity. By analyzing the transcripts of both the host and virus, scRNA-seq enables a better understanding of the delicate balance between the two. The development of this technology contributes to a better understanding of viral pathogenesis and enhances the development of antiviral therapeutic strategies.
SEMINARS IN IMMUNOPATHOLOGY
(2023)
Article
Immunology
Lin Lv, Tianyun Zhang, Hanying Jia, Yanyan Zhang, Asif Ahsan, Xiaoyang Zhao, Teng Chen, Zhiqiang Shen, Ning Shen
Summary: This study investigated the temporal viral and host transcriptomes in porcine alveolar macrophages infected with virulent and attenuated African swine fever virus (ASFV) strains. The findings demonstrated that the attenuated ASFV strain HuB20 can quickly activate the host's antiviral immune response, as well as activating genes associated with RNA virus response in addition to the DNA sensing mechanism.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Balazs Kakuk, Andras Attila Kiss, Gabor Torma, Zsolt Csabai, Istvan Prazsak, Mate Mizik, Klara Megyeri, Dora Tombacz, Zsolt Boldogkoi
Summary: This study utilized nanopore sequencing to investigate the transcriptome structure and gene expression patterns of Vesicular stomatitis Indiana virus, revealing that despite the virus having a simple genome, it displays a complex transcriptomic architecture with differential expression patterns in different cell types.
Article
Microbiology
Bartosz Czech, Yachun Wang, Kai Wang, Hanpeng Luo, Lirong Hu, Joanna Szyda
Summary: Climate change affects animal physiology by reducing animal vitality due to heat stress. This study analyzed the interaction between gene expression and microbiota to identify genes and microbiota associated with physiological changes in cattle under heat stress. These genes and microbiota can be used as biomarkers for heat stress in cattle.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Xiaoting Yao, Tian Jing, Qingru Geng, Ming Pang, Xuanduo Zhao, Shaofei Li, Dekun Chen, Wentao Ma
Summary: Through comprehensive analysis of the transcriptomes of attenuated ORFV strain and goat lip fibroblasts, it was found that the global patterns of immune gene expression induced by different ORFV isolates were mostly shared. However, there were significant differences in host transcripts and viral gene non-synonymous mutations between the wild-type and attenuated ORFV strains. Additionally, the study provided novel insights into the molecular basis of ORFV virulence.
Article
Multidisciplinary Sciences
Zoltan Maroti, Dora Tombacz, Norbert Moldovan, Gabor Torma, Victoria A. Jefferson, Zsolt Csabai, Gabor Gulyas, Akos Dormo, Miklos Boldogkoi, Tibor Kalmar, Florencia Meyer, Zsolt Boldogkoi
Summary: This study utilized third-generation sequencing technology to analyze the effect of bovine alphaherpesvirus type 1 on gene expression in bovine epithelial cells, revealing significant up- and down-regulation of host gene networks by the virus. The study also identified a large number of novel bovine transcript isoforms, demonstrating differential expression of host transcript isoforms in response to viral infection.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Physical
Jingyu Qi, Haibin Zhu, Yijian Li, Xiangyu Guan, Ying He, Guanhua Ren, Qiang Guo, Longqi Liu, Ying Gu, Xuan Dong, Ya Liu
Summary: Cell-cell interaction is an important way for transmitting information and activating the effects of functional cells. Researchers propose a droplet-based microfluidic platform for cell-cell interaction sequencing and screening, which allows for efficient analysis and interpretation of single-cell transcriptomes in cell interactions. This work fills a gap in the current experimental platform for cell-cell interaction research and has significant practical implications in biomedicine.
Article
Parasitology
Xiaoxiang Hu, Xiaolei Liu, Chen Li, Yulu Zhang, Chengyao Li, Yanfeng Li, Yingxi Chen, Heng Guo, Xue Bai, Mingyuan Liu
Summary: The gene expression patterns in response to Trichinella infection in murine myocytes were most pronounced during early infection, with a sharp increase in differentially expressed genes at 10 days post-infection. However, the initial reaction steadied in later stages of infection. This dataset provides valuable insight into how pathogens evade host defenses and survive in the mammalian environment.
PARASITES & VECTORS
(2021)
Article
Neurosciences
Rachel E. McMillan, Ellen Wang, Aaron F. Carlin, Nicole G. Coufal
Summary: Microglia, the resident macrophage of the central nervous system, play important roles in human development, health, and disease. Recent studies have shown that microglia have both protective and detrimental effects in the progression of neurotropic viral infections. Understanding the diversity of human microglial responses is crucial for therapeutic interventions, but modeling human microglia has been challenging due to interspecies differences and rapid transformation in vitro.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Multidisciplinary Sciences
Joseph Hiatt, Judd F. Hultquist, Michael J. McGregor, Mehdi Bouhaddou, Ryan T. Leenay, Lacy M. Simons, Janet M. Young, Paige Haas, Theodore L. Roth, Victoria Tobin, Jason A. Wojcechowskyj, Jonathan M. Woo, Ujjwal Rathore, Devin A. Cavero, Eric Shifrut, Thong T. Nguyen, Kelsey M. Haas, Harmit S. Malik, Jennifer A. Doudna, Andrew P. May, Alexander Marson, Nevan J. Krogan
Summary: This study systematically evaluates the functional roles of 426 genes in HIV replication by using CRISPR-Cas9-mediated knock-out in primary human CD4+ T cells. They identify 86 candidate host factors that alter HIV infection, providing new insights into HIV biology.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Belinda L. Spillings, Christopher J. Day, Albert Garcia-Minambres, Anupriya Aggarwal, Nicholas D. Condon, Thomas Haselhorst, Damian F. J. Purcell, Stuart G. Turville, Jennifer L. Stow, Michael P. Jennings, Johnson Mak
Summary: This study reveals that HIV is captured near the lymphocyte surface in a virion-glycan-dependent manner. Removing certain glycans impairs virus-cell binding and interfering with glycan interactions reduces HIV infectivity. These findings provide new insights into the interaction between HIV and host cells.
Article
Immunology
Luis A. F. Andrade, Alice F. Versiani, Edel F. Barbosa-Stancioli, Jenner K. P. dos Reis, Jordana Grazziela A. C. dos Reis, Flavio G. da Fonseca
Summary: The feline immunodeficiency virus (FIV) is a global retrovirus that affects both domestic and wild cats, and can lead to death. Currently, there is only one effective vaccine for certain FIV subtypes, while another common subtype is not preventable by vaccination. Researchers have developed a new vaccine prototype and conducted preclinical tests in mice, showing potential for further development and use in cats.
Article
Chemistry, Multidisciplinary
Natchaya Rasri, Lueacha Tabtimmai, Charoenkwan Kraiya, Montarop Yamabhai, Chomdao Sinthuvanich, Jatuporn Rattanasrisomporn, Kiattawee Choowongkomon
Summary: A feline IgG-bound scFv was selected and a scFv-AP platform was developed for feline infectious disease detection.
Article
Microbiology
Zheng Zhu, Lvyan Liao, Jiawen Su, Zhenni Liu, Shulei Pan, Yifan Huang, Yijian Wu
Summary: The study revealed that opportunistic pathogens proliferated significantly in Muscovy ducklings infected with MDRV. The immune system of the host actively combats the infection of gut microbes. The interaction between MDRV, intestinal bacteria, and host immune system was analyzed, providing insights into the mechanism of intestinal immunosuppression caused by MDRV.
VETERINARY MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Paola Dezubiria, E. Susan Amirian, Keegan Spera, P. Cynda Crawford, Julie K. Levy
Summary: Approximately 5% of cats in US animal shelters are positive for FeLV or FIV, with over 100,000 positive cats in shelters each year. This study focuses on the retroviral management practices in Florida shelters, which are characterized by chronic pet overpopulation and high shelter admissions.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Milica Krunic, Reinhard Ertl, Benedikt Hagen, Fritz J. Sedlazeck, Regina Hofmann-Lehmann, Arndt von Haeseler, Dieter Klein
BMC VETERINARY RESEARCH
(2015)
Article
Nutrition & Dietetics
Barbara U. Metzler-Zebeli, Evelyne Mann, Reinhard Ertl, Stephan Schmitz-Esser, Martin Wagner, Dieter Klein, Mathias Ritzmann, Qendrim Zebeli
BRITISH JOURNAL OF NUTRITION
(2015)
Article
Virology
Reinhard Ertl, Melanie Korb, Ines Langbein-Detsch, Dieter Klein
Article
Agriculture, Dairy & Animal Science
B. U. Metzler-Zebeli, R. Ertl, D. Gruell, T. Molnar, Q. Zebeli
Article
Reproductive Biology
M. Merkl, R. Ertl, S. Handschuh, C. Aurich, S. Schaefer-Somi
Article
Biotechnology & Applied Microbiology
Juraj Hlavaty, Reinhard Ertl, Ingrid Miller, Cordula Gabriel
BIOMED RESEARCH INTERNATIONAL
(2016)
Article
Multidisciplinary Sciences
Barbara U. Metzler-Zebell, Peadar G. Lawlor, Elizabeth Magowan, Ursula M. McCormack, Tania Curiao, Manfred Hollmann, Reinhard Ertl, Joerg R. Aschenbach, Qendrim Zebell
Article
Veterinary Sciences
J. G. Rushton, R. Ertl, D. Klein, B. Nell
VETERINARY AND COMPARATIVE ONCOLOGY
(2017)
Article
Endocrinology & Metabolism
Barbara U. Metzler-Zebeli, Reinhard Ertl, Dieter Klein, Qendrim Zebeli
Article
Multidisciplinary Sciences
Rohini Chopra-Dewasthaly, Melanie Korb, Rene Brunthaler, Reinhard Ertl
Article
Agriculture, Dairy & Animal Science
Sven Budik, Ingrid Walter, Marie-Christine Leitner, Reinhard Ertl, Christine Aurich
Summary: The mobile preimplantation phase of equine gestation is essential for maternal recognition of pregnancy and relies on prostaglandin synthesis enzymes, with prostaglandins playing a key role in embryonic mobility. The spatial and temporal abundance of these enzymes affects the movement of the embryo, while oxytocin has no effect on key enzyme synthesis.
Article
Agriculture, Dairy & Animal Science
Gustavo D. A. Gastal, Dragos Scarlet, Maria Melchert, Reinhard Ertl, Christine Aurich
Summary: In embryos created through assisted reproductive techniques, changes in gene expression and DNA methylation due to storage temperature differences may impact embryonic development and viability after transfer. This study demonstrates alterations in gene expression and methylation levels of stored horse embryos compared to fresh embryos, highlighting potential risks of compromising embryo development following short-term storage, regardless of temperature.
Article
Virology
Marcha Badenhorst, Armin Saalmueller, Janet M. Daly, Reinhard Ertl, Maria Stadler, Christina Puff, Madeleine de le Roi, Wolfgang Baumgaertner, Michael Engelmann, Sabine Brandner, Hannah K. Junge, Barbara Pratscher, Asisa Volz, Bertrand Saunier, Thomas Krey, Johannes Wittmann, Steffen Heelemann, Julien Delarocque, Bettina Wagner, Daniel Todt, Eike Steinmann, Jessika-M Cavalleri
Summary: Equine hepacivirus (EqHV), the closest genetic relative of hepatitis C virus, lacks an effective vaccine. In this study, the potential of the equine model for hepacivirus vaccine research was investigated. Vaccination with EqHV E2 recombinant protein and subsequent EqHV inoculation were performed on ponies, and their host responses were analyzed. Although the vaccine did not provide complete protection, vaccinated ponies showed faster clearance of EqHV RNA and recovery from liver insult compared to the control group. This study suggests that the equine model holds promise for future hepacivirus vaccine research.
Article
Cell Biology
Natascha Leitner, Reinhard Ertl, Simone Gabner, Andrea Fuchs-Baumgartinger, Ingrid Walter, Juraj Hlavaty
Summary: The study aimed to establish novel canine osteosarcoma cell lines (COS3600, COS3600B, COS4074) and characterize the recently described COS4288 cells. The cell lines differed notably in their biological characteristics, and COS3600B and COS4288 cells showed the ability to produce visible colonies and have the highest migratory capacity, respectively.
Article
Agriculture, Dairy & Animal Science
D. Scarlet, N. Ille, R. Ertl, B. G. Alves, G. D. A. Gastal, S. O. Paiva, M. O. Gastal, E. L. Gastal, C. Aurich
DOMESTIC ANIMAL ENDOCRINOLOGY
(2017)