Review
Biochemistry & Molecular Biology
Eui-Joon Kil, Donghun Kim
Summary: In this review, the destructive nature of the small brown planthopper, Laodelphax striatellus, and its transmission of viruses causing significant loss in rice production are discussed. Research on vector biology, interactions between vectors and pathogens, as well as omics approaches have been conducted to explore novel strategies for control. The roles of saliva proteins, microbial community, insecticide resistance, RSV classification, host range and symptoms, genome composition and virus-derived proteins, distribution, interactions, resistance, and control are explored to propose future directions for integrated pest management.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Agronomy
Chengye Lu, Daoran Jin, Lujie Zhang, Gang Lu, Yinghua Ji, Yijun Zhou, Yunyue Wang, Shuo Li
Summary: This study demonstrates that transgenic rice plants expressing viral glycoproteins can interfere with the acquisition and transmission of Rice stripe virus (RSV) by the small brown planthopper (SBPH). The soluble fraction (NSvs2-N-S) of the viral glycoprotein NSvs2-N was transformed into rice calli, resulting in transgenic plants with the exogenous gene. Feeding SBPH on the transgenic plants before RSV-infected rice or feeding the insects on RSV-infected transgenic plants inhibited virus acquisition by the vectors and reduced subsequent viral titers. Immunofluorescence labeling confirmed the inhibition of viral infection in the insect midgut after SBPH feeding on transgenic plants. In vitro experiments using RSV-infected insect cells further supported the role of NSvc2-N-S in reducing viral infection. The findings suggest that NSvs2-N-S transgenic plants have a negative effect on RSV transmission by insect vectors and provide a novel and effective approach to control plant viral diseases.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Jiangen Wu, Yuanyuan Zheng, Chenxi Xu, Qiqi Jiao, Chenglong Ye, Tongtong Chen, Xiaoping Yu, Kun Pang, Peiying Hao
Summary: This study reveals the importance of transferrin genes NlTsf1, NlTsf2, and NlTsf3 in the interaction between rice and brown planthopper (BPH). RNAi treatment of NlTsf3 significantly reduces the egg production of BPH, while treatments of NlTsf1 and NlTsf2 also affect the survival rate of BPH.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Virology
Jie Li, Wenyu Pan, Shuling Zhao, Changyong Liang
Summary: In this study, we identified three small brown planthopper proteins that interact with the N-terminal 381 amino acids of Rice stripe tenuivirus Pc2 (Pc2N) using a yeast two-hybrid system. Further investigation revealed that heat shock protein cognate 70 (HSC70) interacts with RSV Pc2N and the inhibition of HSC70 expression reduced virus levels in SBPHs, impacting virus transmission efficiency.
ARCHIVES OF VIROLOGY
(2022)
Article
Virology
Lu Tong, Xiaofang Chen, Wei Wang, Yan Xiao, Jinting Yu, Hong Lu, Feng Cui
Summary: Alternative splicing (AS) is a common posttranscriptional regulatory event in eukaryotic organisms, and its effects on insect vectors in response to virus infection are not well understood. In this study, third-generation sequencing technology was used to analyze the AS response of the small brown planthopper to rice stripe virus (RSV). It was found that 31.4% of alternatively spliced genes responded to RSV infection, and one of the c-Jun N-terminal kinase genes showed different isoforms that facilitated viral accumulation in the planthoppers. These results suggest that AS events may play a role in the complex regulatory relationships between viruses and insect vectors.
JOURNAL OF VIROLOGY
(2022)
Article
Plant Sciences
Wintai Kamolsukyeunyong, Wissarut Sukhaket, Kitsada Pitija, Pornwalai Thorngkham, Sugunya Mahatheeranont, Theerayut Toojinda, Apichart Vanavichit
Summary: The rice sesquiterpene synthase II gene plays a role in the antixenosis defense mechanism against brown planthopper infestation, with differences in functionality observed between resistant and susceptible rice varieties. Single-nucleotide polymorphisms in the gene's promoter region and exon 5, as well as a seven amino acid deletion in the protein sequence, may negatively regulate gene function. Analysis suggests a correlation between SNPs in specific genes and the expression of OsSTPS2, while a 21 bp deletion in exon 5 affects the antixenosis mechanism during BPH infestation.
Review
Plant Sciences
Shengli Jing, Jingang Xu, Hengmin Tang, Peng Li, Bin Yu, Qingsong Liu
Summary: This review explores the roles of sRNAs in rice-BPH interactions and highlights recent research progress in unraveling the mechanism of cross-kingdom RNA interference between host plants and insects, as well as the application of this interference in pest management of crops including rice.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Kun Zhang, Xinjian Zhuang, Hongmei Xu, Xiao Guo, Zhen He, Kai Xu, Fang Liu
Summary: This study cloned and expressed the capsid protein gene of rice stripe virus and developed multiple serology-based detection methods using the prepared polyclonal antibody. These methods can simultaneously identify RSV-infected rice plants and SBPHs in wild field conditions, contributing to the control of RSV in rice planting regions.
Article
Agronomy
Lu Jun, Liu Jinhui, Chen Lin, Sun Jiawei, Su Qin, Li Shihui, Yang Jianhua, Zhang Wenqing
Summary: This study reveals the involvement of miRNA in plant defense response against brown planthopper (BPH). The genetic history of rice varieties determines the regulation mode of miRNA and affects their response to BPH-feeding. Furthermore, a potential cross-kingdom miRNA, miR5795, is found to target a gene in BPH, leading to a reduction in BPH oviposition.
Article
Multidisciplinary Sciences
Mengyu Liu, Gaojie Hong, Huijing Li, Xiaoli Bing, Yumeng Chen, Xiangfeng Jing, Jonathan Gershenzon, Yonggen Lou, Ian T. Baldwin, Ran Li
Summary: Plants produce chemical defenses to poison or deter insect herbivores, but these defenses may also affect the beneficial endosymbionts of the herbivores. This study found that rice produces an antifungal flavonoid phytoalexin in response to attack by a pest, which inhibits the pest's beneficial endosymbionts.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Wei Guo, Linlin Du, Chenyang Li, Shuhui Ma, Zhaoyun Wang, Ying Lan, Feng Lin, Yijun Zhou, Yunyue Wang, Tong Zhou
Summary: Research has shown that plant viruses can modify the feeding preference of insect vectors to facilitate the transmission of the viruses. In this study, it was found that rice stripe virus (RSV) enhances its spread by causing its vector, the small brown planthopper (SBPH), to feed more on rice leaves. Furthermore, the leaves were found to be more susceptible to RSV infection compared to the stems. These findings provide insights into how viruses manipulate their vectors for efficient transmission.
Article
Plant Sciences
Xiaozun Li, Jian Zhang, Xinxin Shangguan, Jingjing Yin, Lili Zhu, Jie Hu, Bo Du, Wentang Lv
Summary: The transcription factor OsWRKY71 plays a key role in the Bph15-mediated resistance against the brown planthopper in rice, and three genes have been identified to be involved in this resistance.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Entomology
Amir Zaman Shah, Chao Ma, Yuanyuan Zhang, Qiuxin Zhang, Gang Xu, Guoqing Yang
Summary: This study demonstrates that the use of decoyinine as a seed treatment significantly reduces the fecundity of the small brown planthopper (SBPH) and improves rice resistance. Decoyinine enhances rice defense against SBPH by stimulating plant defensive responses, making it a potential environmentally friendly alternative to chemical pest control.
Article
Biochemistry & Molecular Biology
Xiao Zhang, Daoming Liu, Dong Gao, Weining Zhao, Huaying Du, Zeyu Qiu, Jie Huang, Peizheng Wen, Yongsheng Wang, Qi Li, Wenhui Wang, Haosen Xu, Jun He, Yuqiang Liu, Jianmin Wan
Summary: Plants have a defense system against insect pests, and cytokinin plays a crucial role in enhancing rice resistance against the brown planthopper. The presence of cytokinin promotes the biosynthesis and signaling in response to brown planthopper feeding. This resistance is dependent on the plant hormone jasmonic acid.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shaojie Shi, Huiying Wang, Lingyun Nie, Di Tan, Cong Zhou, Qian Zhang, Yi Li, Bo Du, Jianping Guo, Jin Huang, Di Wu, Xiaohong Zheng, Wei Guan, Junhan Shan, Lili Zhu, Rongzhi Chen, Longjian Xue, Linda L. Walling, Guangcun He
Summary: The resistance of rice plants to the brown planthopper is associated with the fortification of the sclerenchyma tissue, which prevents the insects from reaching the phloem for feeding. The discovery of the resistance gene Bph30 and its role in enhancing cellulose and hemicellulose synthesis sheds light on a novel mechanism for controlling planthoppers in rice. This fortified sclerenchyma-mediated resistance mechanism expands our understanding of plant-insect interactions.