Article
Plant Sciences
Neha Gupta, Kishorekumar Reddy, Prabu Gnanasekaran, Ying Zhai, Supriya Chakraborty, Hanu R. Pappu
Summary: The beta V1 protein encoded by radish leaf curl betasatellite (RaLCB) induces cell death and hypersensitive response (HR) in Nicotiana benthamiana, accompanied by increased accumulation of reactive oxygen species (ROS) and HR-related transcripts. The protein localizes to the cellular periphery and interacts with replication enhancer protein (AC3) of the helper virus.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Cell Biology
Moshik Shteinberg, Ritesh Mishra, Ghandi Anfoka, Miassar Altaleb, Yariv Brotman, Menachem Moshelion, Rena Gorovits, Henryk Czosnek
Summary: Research indicates a positive interaction between viruses and plants, with Tomato yellow curl virus (TYLCV) enhancing tolerance to drought in tomato plants. TYLCV-resistant tomato plants infected with the virus can survive long periods without water and produce fruits similar to control plants after recovery.
Article
Plant Sciences
Dirk Janssen, Almudena Simon, Maher Boulares, Leticia Ruiz
Summary: Transmission parameters of ToLCNDV-ES differ between zucchini and tomato plants, with significantly higher transmission efficiency in zucchini. This may explain the different incidences of ToLCNDV-ES in zucchini and tomato crops in Spain. The results are also important for virus management and the trade and transport of infected plant material.
Article
Agronomy
Premchand Udavatha, Raghavendra K. Mesta, Mantapla Puttappa Basavarajappa, Venkataravanappa Venkataravanappa, Venkatappa Devappa, Lakshminarayana Reddy C. Narasimha Reddy, Kodegandlu Subbanna Shankarappa
Summary: Papaya is an important fruit crop in tropical and subtropical regions, and papaya leaf curl disease is a major concern. A survey in Karnataka, India, found that the disease incidence ranged from 10% to 21%. Genetic analysis revealed the presence of variants of Chilli leaf curl virus and other begomoviruses.
Article
Plant Sciences
V. Venkataravanappa, K. Ashwathappa, H. D. Vinaykumar, C. N. Lakshminarayana Reddy, K. S. Shankarappa, M. Krishna Reddy
Summary: Crotalaria juncea L., known as sunhemp, is a tropical legume plant with good potential for nitrogen fixation and drought tolerance. However, it can act as an alternative host for begomoviruses, with sequencing analysis showing a high sequence identity between the virus and associated DNA satellites.
JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Asifa Khan, Sana Tabanda Saeed, Soumya Sinha, Sujata Singh Yadav, Abdul Samad
Summary: Withania somnifera, an important medicinal plant, was found to be infected by a novel begomovirus, named Withania leaf curl virus, causing severe symptoms and economic losses in India.
Article
Plant Sciences
Susheel Kumar, Rashmi Raj, Lalit Agrawal, Taruna Gupta, Shri Krishna Raj, Meraj Jaidi, Ashish Srivastava
Summary: The study identified severe leaf curl symptoms on radish plants in cultivated fields in Lucknow, caused by a begomovirus and its associated betasatellite transmitted by whiteflies. Molecular analysis showed close phylogenetic relationships with papaya leaf curl virus (PaLCuV) and its betasatellite (PaLCuB).
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Zhiyuan Wang, Yaqin Wang, Rosa Lozano-Duran, Tao Hu, Xueping Zhou
Summary: A novel viral protein C6 encoded by begomoviruses has been discovered, which possesses an internal mitochondrial targeting signal and can localize to plant cell organelles.
PHYTOPATHOLOGY RESEARCH
(2022)
Article
Plant Sciences
Ho-Hsiung Chang, Deri Gustian, Chung-Jan Chang, Fuh-Jyh Jan
Summary: Understanding the seedborne nature of plant viruses is crucial for disease control strategies and seed market. This study revealed that ToLCNDV, ToLCTV, and TYLCTHV can be transmitted through seeds or pollens of cucumber and tomato plants.
Article
Virology
Juliana O. Souza, Tomas A. Melgarejo, Sandra Vu, Erich Y. T. Nakasu, Li-Fang Chen, Maria R. Rojas, F. Murilo Zerbini, Alice K. Inoue-Nagata, Robert L. Gilbertson
Summary: This study describes the molecular and biological properties of tomato mottle leaf curl virus (ToMoLCV) from Brazil and establishes that it is a NW monopartite begomovirus indigenous to northeastern Brazil. It answers a long-standing question regarding the genome of this virus and provides insights into the origin and evolution of Brazilian begomoviruses.
JOURNAL OF VIROLOGY
(2022)
Article
Agronomy
Nasim Ahmed, Syed Shan-e-Ali Zaidi, Imran Amin, Brian E. Scheffler, Shahid Mansoor
Summary: The global spread of Tomato yellow leaf curl virus poses a threat to tomato production in tropical and sub-tropical regions, with a new species Tomato leaf curl Oman virus identified in Oman. In Pakistan, Tomato leaf curl disease symptoms were observed in tomato plants, with the presence of ToLCOMV-PK and associated ToLCB causing enhanced symptoms and virus accumulation in Nicotiana benthamiana plants.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Horticulture
Abhinav Kumar, Nishtha Kashyap, Rohit Gupta
Summary: Papaya leaf curl disease poses a serious threat to papaya production in India, and can also infect chili and tomato plants. The study identified a virus from infected papaya plants that shared nucleotide sequences with chili leaf curl virus and tomato leaf curl virus in different parts of India. Additionally, the study demonstrated biological and molecular characteristics of a recombinant ChiLCV isolated from papaya plants.
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Muhammad Irfan Siddique, Joung-Ho Lee, Jung-Hwan Ahn, Meirina Kartika Kusumawardhani, Ramadhani Safitri, Asep Harpenas, Jin-Kyung Kwon, Byoung-Cheorl Kang
Summary: This study evaluated the resistance of pepper to Pepper yellow leaf curl virus (PepYLCV) using augmented inoculation and constructed a high-density linkage map to identify QTLs associated with resistance. Three QTLs were identified, and candidate genes associated with resistance were inferred. Single markers derived from the QTLs were developed and validated in other populations and commercial varieties.
Article
Plant Sciences
Tahir Farooq, Qi Lin, Xiaoman She, Ting Chen, Zhenggang Li, Lin Yu, Guobing Lan, Yafei Tang, Zifu He
Summary: Begomoviruses, highly pathogenic plant viruses, are economically important and contribute significantly to global crop diseases. This study investigated the molecular mechanisms underlying the interactions between begomoviruses and their whitefly vector (Bemisia tabaci). The researchers found that a specific whitefly species efficiently transmitted the virus, while another species did not. They identified a protein in the whitefly that interacts with the virus and found that viral infection suppressed the transcription of an innate immunity-related gene in the whitefly, which enhanced virus accumulation. The study provides insights into the complex interactions between begomoviruses and their vector and highlights the importance of innate immunity in virus transmission.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Sara Shakir, Georg Jander, Nazia Nahid, Muhammad Mubin, Ayesha Younus, Muhammad Shah Nawaz-ul-Rehman
Summary: The Rep proteins of pathogenic begomoviruses interact with the DNA replication machinery of their eukaryotic hosts, showing 13-28% sequence variation among CLCuMuV and PeLCV isolates. Although phylogenetic clusters can separate based on the country of origin, interactions with PCNA proteins are strongly conserved across different host species.
Article
Virology
Fenisha D. Chahwala, Dhananjay Singh, R. Vinoth Kumar, Sangeeta Rathore, Brijesh K. Yadav, Bijendra Singh, Achuit K. Singh
Summary: Begomoviruses, transmitted by whiteflies, can cause severe diseases in economically important crops and non-cultivated plants, with non-cultivated weeds serving as natural virus reservoirs. A new monopartite begomovirus was discovered in Gujarat, India, in January 2016, indicating that weeds may harbor a complex of begomovirus-alphasatellite-betasatellite and serve as a potential source of virus inoculum.
ARCHIVES OF VIROLOGY
(2021)
Article
Plant Sciences
Ram Krishna, Waquar Akhter Ansari, Durgesh Kumar Jaiswal, Achuit Kumar Singh, Jay Prakash Verma, Major Singh
Summary: This study aimed to enhance drought tolerance and yield potential in transgenic tomato plants by co-overexpressing the AtDREB1A and BcZAT12 genes, resulting in improved physiological indexes and growth attributes under water deficit conditions. The findings provide a basis for genetic engineering in plants through a multigenic transgenic approach to tackle various biotic and abiotic stresses.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Achuit K. Singh, Brijesh K. Yadav, Ram Krishna, R. Vinoth Kumar, Gyan P. Mishra, Suhas G. Karkute, Nagendran Krishnan, Tania Seth, Shweta Kumari, Bijendra Singh, Prabhakar M. Singh, Jagdish Singh
Summary: This study reports the association of a monopartite begomovirus (bhendi yellow vein mosaic virus; BYVMV) and betasatellite (bhendi yellow vein mosaic betasatellite; BYVB) with OELCuD in the Mau region of Uttar Pradesh, India. Co-inoculation of BYVMV and BYVB resulted in increased symptom severity and enhanced BYVMV accumulation. This is the first study that satisfies Koch's postulates for OELCuD in its natural host, which is significant in understanding the impact on virus pathogenesis and disease development.
Article
Virology
Sangeeta, Punam Ranjan, R. Vinoth Kumar, Bhavin S. Bhatt, Fenisha D. Chahwala, Brijesh K. Yadav, Sunita Patel, Bijendra Singh, Achuit K. Singh
Summary: Identified two distinct monopartite begomovirus and betasatellite complexes causing Tomato leaf curl disease in western India, demonstrating they are monopartite begomoviruses. Detection of recombination events in cloned begomoviruses suggests potential virus resistance breaking disease complexes in India.
Article
Biotechnology & Applied Microbiology
Suhas Gorakh Karkute, Waquar Akhter Ansari, Achuit Kumar Singh, Prabhakar Mohan Singh, Nagendra Rai, Anant Bahadur, Jagdish Singh
Summary: This study characterized two tomato genotypes for stress tolerance and identified several stress-responsive genes that were significantly up-regulated after high-temperature stress exposure. These candidate genes can be used for developing gene-based markers and marker-assisted breeding, confirming the rapid response of the genotypes to high-temperature stress and establishing them as potential candidates for stress tolerance improvement programs.
Article
Plant Sciences
Ram Krishna, Waquar Akhter Ansari, Durgesh Kumar Jaiswal, Achuit Kumar Singh, Ram Prasad, Jay Prakash Verma, Major Singh
Summary: Double transgenic tomato plants developed by pyramiding AtDREB1 and BcZAT12 genes showed significant drought tolerance and increased yield potential; this study lays the foundation for a multigene transgenic approach to cope with drought stress in tomato.
PLANT CELL REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Pratap Adinath Divekar, Srinivasa Narayana, Bhupendra Adinath Divekar, Rajeev Kumar, Basana Gowda Gadratagi, Aishwarya Ray, Achuit Kumar Singh, Vijaya Rani, Vikas Singh, Akhilesh Kumar Singh, Amit Kumar, Rudra Pratap Singh, Radhe Shyam Meena, Tusar Kanti Behera
Summary: Plants have evolved adaptive strategies to defend against herbivores by synthesizing plant secondary metabolites (PSMs). PSMs play important roles in detecting and responding to herbivore attacks, and have limited adaptability to insects. They cause direct toxicity to pests and indirectly protect plants by attracting herbivore natural enemies. Understanding the molecular regulation of PSM biosynthesis can contribute to improving plant tolerance to herbivores.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Asha Lata Singh, Sneha Chaudhary, Sunil Kumar, Aniruddha Kumar, Achuit Singh, Akhilesh Yadav
Summary: This study successfully degraded and decolorized RY-145 dye using a mixed bacterial consortium of E. asburiae and E. cloacae. The optimal conditions for maximum degradation and decolorization were determined. Biochemical analysis identified several compound metabolites with industrial applications. Toxicity tests and genotoxicity studies demonstrated the reduced toxicity of the treated dye.
Article
Biotechnology & Applied Microbiology
Nagendran Krishnan, B. Rajasekhar Reddy, Shweta Kumari, Achuit Kumar Singh
Summary: In this study, a Begomovirus associated with golden mosaic disease on vegetable cowpea was characterized. The genomic components were cloned and sequenced, showing high similarity to mungbean yellow mosaic India virus previously reported from India. Phylogenetic analysis suggested a common ancestral relationship between the study isolate and MYMIV isolates from India, Pakistan, and Nepal. Recombination analysis indicated that this cowpea isolate likely evolved through recombination of MYMIV sequences. Additionally, Agrobacterium-based dimeric clone constructs were found to be highly infectious on cowpea host, causing typical golden mosaic symptoms.
Review
Plant Sciences
Jagesh Kumar Tiwari, Suresh Reddy Yerasu, Nagendra Rai, Dhananjaya P. Singh, Achuit K. Singh, Suhas G. Karkute, Prabhakar M. Singh, Tusar K. Behera
Summary: Tomatoes are important vegetable crops, and genomics-assisted breeding shows great potential in this field. With the rapid development and cost reduction of sequencing technologies, as well as the use of high-throughput genotyping platforms and bioinformatics tools, the tomato genome has been decoded and extensively studied, providing valuable information for breeding programs. Genomics-assisted breeding has made progress in identifying genes and SNP markers associated with agronomic traits, and the genomic selection method is expected to improve breeding efficiency and genetic gain.
CRITICAL REVIEWS IN PLANT SCIENCES
(2022)
Article
Plant Sciences
Ram Krishna, Durgesh Kumar Jaiswal, Waquar Akhter Ansari, Saurabh Singh, P. S. Soumia, Achuit Kumar Singh, Babita Kumari, Major Singh, Jay Prakash Verma
Summary: This study investigates the use of hexa plant growth-promoting microorganisms (PGPM) to improve the growth and yield of tomato plants under drought stress. The inoculation of hexa-PGPM consortium to the plants helps enhance plant growth, reduce cellular damage, and increase antioxidant enzyme activity. The gene expression analysis also shows up-regulation of stress-responsive genes. Moreover, the application of hexa-PGPM improves the physical, chemical, and biological properties of soil under drought stress conditions.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2022)
Article
Genetics & Heredity
Vemula Chandra Kaladhar, Yeshveer Singh, Athira Mohandas Nair, Kamal Kumar, Achuit Kumar Singh, Praveen Kumar Verma
Summary: This study identified a key virulence factor, BsCE66, in the fungal pathogen Bipolaris sorokiniana, which causes the devastating Spot Blotch disease in wheat plants. BsCE66 triggers oxidative burst and cell death in the host plant, contributing to disease progression and immune modulation.
FUNGAL GENETICS AND BIOLOGY
(2023)
Article
Virology
Vinoth Sangeeta, R. Vinoth Kumar, Brijesh K. Yadav, Bhavin S. Bhatt, Ram Krishna, Nagendran Krishnan, Suhas G. Karkute, Sudhir Kumar, Bijendra Singh, Achuit Singh
Summary: In the western part of India, a study on the characterization of virus complexes with tomato leaf curl disease (ToLCD) caused by begomoviruses was conducted. A complex of 19 DNA-A and 4 DNA-B begomoviruses as well as 15 betasatellites associated with ToLCD was identified. The study also discovered a novel betasatellite and an alphasatellite, and detected recombination breakpoints in the cloned begomoviruses and betasatellites. The findings suggest the potential of these virus complexes in breaking disease resistance and expanding their host range, highlighting the need to investigate the interaction mechanism between resistance-breaking virus complexes and infected hosts.
Article
Plant Sciences
Nagendran Krishnan, Shweta Kumari, Tribhuvan Chaubey, R. Vinoth Kumar, Manimurugan Chinnappa, Vikas Dubey, Koshlendra Kumar Pandey, Jagdish Singh, Achuit Kumar Singh
Summary: Mosaic disease caused by begomovirus is a major problem for farmers cultivating sponge gourd in India. We identified a bipartite begomovirus, tomato leaf curl New Delhi virus (ToLCNDV), in sponge gourd samples. Additionally, we detected the presence of tomato leaf curl New Delhi alphasatellite and tobacco leaf curl Patna betasatellite. We also confirmed the seed transmissible nature of ToLCNDV and identified two moderately resistant lines through field screening.
JOURNAL OF PLANT PATHOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Ram Krishna, Waquar Akhter Ansari, P. S. Soumia, Akhilesh Yadav, Durgesh Kumar Jaiswal, Sudhir Kumar, Achuit Kumar Singh, Major Singh, Jay Prakash Verma
Summary: Tomato production is greatly affected by abiotic stresses, especially drought, leading to significant yield loss. Lack of drought tolerance genes in cultivated tomato makes it vulnerable to drought stress. Biotechnological methods, including transgenic technology and CRISPR-Cas, are used to enhance drought tolerance by exploiting non-host genes and editing host tomato genes. This review provides updated information on biotechnological intervention in tomato for drought stress management.