Article
Biochemistry & Molecular Biology
Mohamad Ismail Sultan-Alolama, Amr Amin, Khaled A. El-Tarabily, Ranjit Vijayan
Summary: This study reports the isolation of a phage, UAE_MI-01, from bird feces for the first time, indicating birds as a potential source of phages. The phage was found to be lytic to Verotoxin-producing Escherichia coli O157:H7 and showed stability under varying conditions. The presence of the phage suggests the presence of the host bacteria in wild birds, making it important to investigate other bird species for this pathogenic bacterium.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Chemical
Firuze Ergin
Summary: The effects of freeze drying, spray drying, and electrospraying on the properties of powders containing phage Felix O1 were investigated. Powders produced by spray drying and electrospraying showed lower loss in activity compared to powders produced by freeze drying.
Article
Food Science & Technology
Ya-Ke Li, Hu Chen, Mei Shu, Chan Zhong, Yan Bi, Huan-Huan Yang, Guo-Ping Wu
Summary: The alkali-resistant phage JN01 showed lytic activities against multiple E. coli strains, including multidrug resistant strains. It exhibited stability under stressed conditions and its genome did not contain known virulence-associated or antibiotic-related genes. Treatment with JN01 reduced viable cell counts of E. coli O157:H7 in milk and raw beef samples, suggesting its potential as a biological control agent for E. coli O157:H7 in food.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mohamad Ismail Sultan-Alolama, Amr Amin, Ranjit Vijayan, Khaled A. A. El-Tarabily
Summary: In this study, a virulent bacteriophage named Ec_MI-02 was isolated from the feces of a wild pigeon in the UAE, which can be used as a bio-preservative or in phage therapy. The phage was found to infect multiple serotypes of E. coli O157:H7. The research provides evidence that wild birds may serve as natural reservoirs for phages without antibiotic resistance genes, and studying the genetic makeup of phages that infect human pathogens is crucial for their safe usage in the food industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Do-Won Park, Jong-Hyun Park
Summary: BECP10, a novel bacteriophage targeting E. coli O157 strains, shows strong antibacterial activity and can effectively reduce the pathogen levels in contaminated food matrices over a long period of time, making it a promising biocontrol agent.
Article
Biochemistry & Molecular Biology
Maria Kornienko, Dmitry Bespiatykh, Maja Malakhova, Roman Gorodnichev, Nikita Kuptsov, Egor Shitikov
Summary: Phage therapy is a hopeful approach to combat multidrug-resistant bacteria. However, it requires the isolation of new phages or the search for suitable phages in existing libraries. This study proposes a simple PCR method to differentiate between two families of Staphylococcus phages and eleven genera of Klebsiella phages, allowing for rapid screening and identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Danielle L. Peters, Carly M. Davis, Greg Harris, Hongyan Zhou, Philip N. Rather, Sabahudin Hrapovic, Edmond Lam, Jonathan J. Dennis, Wangxue Chen
Summary: The world is currently facing a global health crisis due to the rapid increase in antimicrobial-resistant bacterial infections. Acinetobacter baumannii, a Gram-negative bacterium with many intrinsic antibiotic resistance mechanisms and the ability to quickly acquire new resistance determinants, is one of the most concerning pathogens. Phage therapy, especially the myoviruses DLP1 and DLP2, shows potential as a treatment option against A. baumannii infections. These phages have a limited host range and exhibit different burst sizes, latency periods, and virulence indices.
Article
Microbiology
Cecile Philippe, Amel Chaib, Fety Jaomanjaka, Olivier Claisse, Patrick M. Lucas, Johan Samot, Christian Cambillau, Claire Le Marrec
Summary: This study reports the first group of virulent oenophages named Vinitor isolated from the winemaking environment. Vinitor phages, morphologically similar to siphoviruses infecting other LAB, are more abundant in musts than temperate oenophages during winemaking. Genomic analysis of Vinitor phages reveals unique combinations of modules and possible evolutionary connections with (pro)phages associated with other bacteria.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Virology
Nikita Kuptsov, Maria Kornienko, Dmitry Bespiatykh, Roman Gorodnichev, Ksenia Klimina, Vladimir Veselovsky, Egor Shitikov
Summary: In the process of bacteriophage infection in Staphylococcus aureus, most of the differentially expressed genes (DEGs) are involved in nucleotide and amino acid metabolism, as well as in cell death prevention. Some virulence factors are overexpressed at the late infection stage, which is of significance for the use of bacteriophages in therapy.
Article
Food Science & Technology
Yuxin Wang, Jing Li, Yang Wang, Jiaoling Wu, Xuhang Wang, Feng Xue, Jianluan Ren, Jianjun Dai, Fang Tang
Summary: The foodborne pathogen E. coli O157:H7 poses a serious threat to human life and health. The emergence of bacterial resistance and limitations of traditional food additives have created an unprecedented risk to human food safety. Therefore, there is an urgent need for new food additives that effectively eliminate Shiga toxin producing E. coli (STEC) O157:H7 from food and food processing equipment. Bacteriophage, a highly versatile and safe option in the food chain, has gained renewed attention. This study developed a novel phage cocktail that can effectively remove STEC O157:H7 from liquid food, solid food, and food processing materials simultaneously, without altering the appearance and nutritional content. This study presents a new strategy of utilizing phage cocktail as a food preservative to safeguard human food safety.
Article
Virology
Jumpei Fujiki, Shin-ichi Yoshida, Tomohiro Nakamura, Keisuke Nakamura, Yurika Amano, Keita Nishida, Keitaro Nishi, Michihito Sasaki, Tomohito Iwasaki, Hirofumi Sawa, Hitoshi Komatsuzawa, Hiroshi Hijioka, Hidetomo Iwano
Summary: This study reported the discovery of a virulent bacteriophage Phi SG005 against Streptococcus gordonii from drainage water, showing efficient bactericidal effects and no prophage-related elements. These findings contribute to the understanding of streptococcus viruses and the development of phage therapy against S. gordonii infection.
Article
Food Science & Technology
Kai-Sheng Shen, Mei Shu, Meng-Xuan Tang, Wen-Yu Yang, Shu-Chao Wang, Chan Zhong, Guo-Ping Wu
Summary: This study cloned, expressed, and characterized an endolysin named LysJN01 from bacteriophage JN01. The recombinant LysJN01 showed strong lytic activity against E. coli O157:H7 and exhibited broad-spectrum antimicrobial activity against Gram-negative pathogens. Additionally, it significantly reduced the count of E. coli O157:H7 on contaminated lettuce.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Immunology
Gunaraj Dhungana, Roshan Nepal, Madhav Regmi, Rajani Malla
Summary: The study demonstrated that oKp_Pokalde_002 rapidly distributed in the mouse model and significantly reduced bacterial count in the blood and other organs within 24 hours, showing promising therapeutic potential for carbapenem-resistant Klebsiella pneumoniae infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Yan Zhou, Qiyang Wan, Hongduo Bao, Yonghao Guo, Shujiao Zhu, Hui Zhang, Maoda Pang, Ran Wang
Summary: Phage SQ17 is a broad-host range lytic phage capable of infecting various E. coli strains, with a short latent period, high burst size, and stability. Experimental results demonstrate that SQ17 can effectively control the counts of EHEC O157:H7 and ETEC in milk, raw beef, and fresh lettuce.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Sangbin Kim, Yoonjee Chang
Summary: The use of antibiotics in the food industry is being replaced by bacteriophage due to the increase of antibiotic-resistant bacteria. In this study, the Salmonella Enter-itidis phage PBSE191 was characterized and applied to a polyvinyl alcohol (PVA) film. The phage showed rapid and strong lytic activity against a broad range of Salmonella isolates. Furthermore, it remained stable in a PVA solution and demonstrated antibacterial performance in both in vitro and in food tests.
FOOD RESEARCH INTERNATIONAL
(2022)
Correction
Virology
Stuart G. Siddell, Peter J. Walker, Elliot J. Lefkowitz, Arcady R. Mushegian, Bas E. Dutilh, Balazs Harrach, Robert L. Harrison, Sandra Junglen, Nick J. Knowles, Andrew M. Kropinski, Mart Krupovic, Jens H. Kuhn, Max L. Nibert, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Arvind Varsani, Francisco Murilo Zerbini, Andrew J. Davison
ARCHIVES OF VIROLOGY
(2020)
Article
Virology
Stuart G. Siddell, Peter J. Walker, Elliot J. Lefkowitz, Arcady R. Mushegian, Bas E. Dutilh, Balazs Harrach, Robert L. Harrison, Sandra Junglen, Nick J. Knowles, Andrew M. Kropinski, Mart Krupovic, Jens H. Kuhn, Max L. Nibert, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Arvind Varsani, Francisco Murilo Zerbini, Andrew J. Davison
ARCHIVES OF VIROLOGY
(2020)
Article
Genetics & Heredity
Jinwei Suo, Heng Zhang, Qi Zhao, Nan Zhang, Yongxue Zhang, Ying Li, Baohua Song, Juanjuan Yu, Jianguo Cao, Tai Wang, Ji Luo, Lihai Guo, Jun Ma, Xumin Zhang, Yimin She, Lianwei Peng, Weimin Ma, Siyi Guo, Yuchen Miao, Sixue Chen, Zhi Qin, Shaojun Dai
GENOMICS PROTEOMICS & BIOINFORMATICS
(2020)
Article
Chemistry, Analytical
Yi-Min She, Roger Y. Tam, Xuguang Li, Michael Rosu-Myles, Simon Sauve
ANALYTICAL CHEMISTRY
(2020)
Article
Infectious Diseases
Johannes Wittmann, Dann Turner, Andrew D. Millard, Padmanabhan Mahadevan, Andrew M. Kropinski, Evelien M. Adriaenssens
Article
Multidisciplinary Sciences
Zhijuan Chen, Vanessa Lancon-Verdier, Christine Le Signor, Yi-Min She, Yun Kang, Jerome Verdier
Summary: This study used the genetic diversity in Medicago truncatula to identify genes/loci regulating seed traits through a genome-wide association study, revealing a strong positive correlation between seed size and protein content, as well as multiple QTNs associated with different seed phenotypes. The findings represent valuable resources for improving legume grain production in species such as pea, common bean, and soybean.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Dann Turner, Andrew M. Kropinski, Evelien M. Adriaenssens
Summary: Bacteriophage taxonomy has been undergoing significant changes in recent years, with the abolition of certain classification units and the establishment of guidelines for delineating species, genus, subfamily, and family-level ranks in tailed phage taxonomy.
Article
Plant Sciences
Ryan Kilburn, Suzanne A. Gerdis, Yi-Min She, Wayne A. Snedden, William C. Plaxton
Summary: Phosphoenolpyruvate carboxylase (PEPC) plays a crucial role in plant metabolism. In castor oil seeds, a bacterial-type PEPC forms a complex with Ricinus communis Ca2+-dependent protein kinase-1 (RcCDPK1) which regulates its activity through phosphorylation. The autophosphorylation of RcCDPK1 facilitates its interaction with PEPC and regulates its transphosphorylation activity.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yi-Min She, Kody Klupt, Grayson Hatfield, Zongchao Jia, Roger Y. Tam
Summary: Mannosidases are crucial enzymes that play diverse roles in cellular processes. We discovered unexpected β1-4-galactosidase activity in the α1-6-mannosidase, providing insights into its dual glycosidase specificity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Jianjun Zhang, Yang Xia, Dinghe Wang, Yamin Du, Yongwu Chen, Congcong Zhang, Juan Mao, Muyang Wang, Yi-Min She, Xinxiang Peng, Li Liu, Josef Voglmeir, Zuhua He, Linchuan Liu, Jianming Li
Summary: This study demonstrates that in rescuing bri1-5 protein degradation, AtEDEM1 plays a predominant role over AtEDEM2, while a mutation in ALG3 eliminates the inhibitory effect of irb1-1 on bri1-5 ERAD.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Virology
Janet T. Lin, Sarah Kirst, Stevan Cucic, Alexandra Klem, Yi-Min She, Andrew M. Kropinski, Hany Anany
Summary: A novel bacteriophage with high specificity and a long latent period was isolated from EHEC-enriched sewage, expanding our understanding of bacteriophage diversity.
Article
Multidisciplinary Sciences
Yi-Min She, Shaojun Dai, Roger Y. Tam
Summary: Glycosylation is an important attribute for assessing the manufacturing quality of mAbs. We developed a highly sensitive LC MS/MS method for comprehensive characterization of low-abundance non-human glycans and their structural isomers. Our findings reveal the structural diversity of non-human glycans and the sulfation of alpha-galactosylated glycans, providing an analytical method and candidate structures for quality control of therapeutic mAb products.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Thomas S. Postler, Luisa Rubino, Evelien M. Adriaenssens, Bas E. Dutilh, Balazs Harrach, Sandra Junglen, Andrew M. Kropinski, Mart Krupovic, Jiro Wada, Anya Crane, Jens H. Kuhn, Arcady Mushegian, Janis Rumnieks, Sead Sabanadzovic, Peter Simmonds, Arvind Varsani, F. Murilo Zerbini, Julie Callanan, Lorraine A. Draper, Colin Hill, Stephen R. Stockdale
Summary: The International Committee on Taxonomy of Viruses has adopted a binomial naming format for virus species, which is comparable with other biological taxonomies. Latinization of these names is optional, but it provides the advantage of cultural neutrality and compatibility with other taxonomies.
JOURNAL OF GENERAL VIROLOGY
(2022)
Article
Microbiology
Dann Turner, Evelien M. Adriaenssens, Igor Tolstoy, Andrew M. Kropinski
Summary: Before submitting a bacteriophage genome sequence to the International Nucleotide Sequence Database Collaboration or writing a publication, it is important to address 14 questions to ensure an accurate and comprehensive annotation of the genome.
PHAGE-THERAPY APPLICATIONS AND RESEARCH
(2021)
Article
Virology
Cristina Moraru, Arvind Varsani, Andrew M. Kropinski
