Article
Microbiology
Qian Du, Xuezhi Zhang, Ning Xu, Mengyu Ma, Bicheng Miao, Yong Huang, Dewen Tong
Summary: This study identified CCT5 as a host interacting protein of PPV NS1 and showed that CCT5 mediates the interaction of PPV NS1 and COPE, regulating viral replication.
VETERINARY MICROBIOLOGY
(2022)
Article
Virology
Jonathan L. Sanchez, Niloofar Ghadirian, Nancy C. Horton
Summary: This paper presents two new structures of the N-terminal domain of the main replication protein, NS1, from human parvovirus B19 (B19V). The NS1-nuc domain plays a crucial role in the rolling hairpin replication of the B19V DNA genome by recognizing origin of replication sequences in double-stranded DNA and cleaving single-stranded DNA at the terminal resolution site (trs). The high-resolution structures of NS1-nuc provide insights into DNA recognition and cleavage by B19V NS1-nuc and predict the important residues involved in DNA cleavage and sequence-specific recognition at the viral origin of replication.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Massimo De Paschale, Claudia Pavia, Teresa Cerulli, Debora Cagnin, Maria T. Manco, Luisa Belvisi, Alessia Paganini, Laura Pogliani, Elena Ceriani, Alessandro Porta, Luciana Parola, Paola Mirri, Bianca Osnaghi, Laura Vismara, Pierangelo Clerici
Summary: Parvovirus B19 (B19V) infection in pregnancy is common in Italy, with higher IgG seroprevalence among Italian women compared to non-Italian women. The prevalence of viremia is low and does not lead to significant fetal damage at birth.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Virology
Alessandro Reggiani, Andrea Avati, Francesca Valenti, Erika Fasano, Gloria Bua, Elisabetta Manaresi, Giorgio Gallinella
Summary: In this study, a derived B19V minigenome was designed and produced, which was capable of replication, transcription, and production of NS1 protein in cells, and could complement defective genomes. This provides a valuable tool for studying viral expression and genetics.
Review
Microbiology
Ignacio Sanchez-Moguel, Carmina Montiel, Ismael Bustos-Jaimes
Summary: Virus-like particles (VLPs) retain cellular recognition properties but are non-infectious, making them promising as medical tools. The VLPs of Parvovirus B19 (B19V) can be produced through heterologous expression and in-vitro assembly of its structural proteins. Chimeric forms of VP2 can be created to carry peptides or functional proteins on the VLPs' surface, without compromising their ability to form VLPs. This review discusses the engineering considerations and potential applications of these multifunctional VLPs.
Article
Virology
Kang Ning, Anuradha Roy, Fang Cheng, Peng Xu, Steve Kleiboeker, Carlos R. Escalante, Jingxin Wang, Jianming Qiu
Summary: This study developed a high-throughput screening assay for identifying antivirals against B19V replication and discovered a purine derivative that exhibited significant antiviral activity against B19V infection.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Gloria Bua, Pasquale Marrazzo, Elisabetta Manaresi, Chiara Gamberini, Laura Bonsi, Francesco Alviano, Giorgio Gallinella
Summary: Both BM-MSCs and FM-MSCs can be infected by B19V, although they cannot support viral replication, they allow persistence of the virus in the infected cultures. Further studies are needed to understand the potential role of MSCs in B19V transmission and the conditions that can favor a potential reactivation of the virus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Dongxin Zeng, Junwen Zheng, Shuwen Feng, Panpan Fan, Dongchi Zhao
Summary: This study demonstrates that B19 NS1 protein negatively regulates GATA1 expression and induces the expression of Hes1/Hes5, thereby interfering with the Notch signaling pathway and potentially playing a role in the negative regulation of hematopoietic cell differentiation.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Article
Biochemistry & Molecular Biology
Yoshito Abe, Yohei Ikeda, Saki Fujiyama, R. Manjunatha Kini, Tadashi Ueda
Summary: This study confirmed the importance of His26 in PriB for replication restart using plasmid complementation and examined the structure of the PriB-DnaT complex through NMR analysis. By evaluating the PriB-DnaT peptide complex model prepared by in silico docking and molecular dynamic simulation, a structural model was proposed that sheds light on the PriB-DnaT interaction.
Article
Biochemistry & Molecular Biology
Felicitas Escher, Ganna Aleshcheva, Heiko Pietsch, Christian Baumeier, Ulrich M. Gross, Benedikt Norbert Schrage, Dirk Westermann, Claus-Thomas Bock, Heinz-Peter Schultheiss
Summary: This study analyzed the impact of transcriptionally active cardiotropic B19V infection on long-term outcomes in adult patients with non-ischemic cardiomyopathy. The results showed that transcriptionally active B19V infection combined with inflammation was the strongest predictor of impaired survival. The findings suggest that advanced diagnostic differentiation of B19V-positive patients is of high prognostic importance and transcriptionally active B19V infection is an unfavorable prognostic trigger of adverse outcome.
Article
Infectious Diseases
Trine H. Mogensen, Morten Kelder Skouboe, Ingolf Molle
Summary: Human infection with parvovirus B19 can lead to various clinical manifestations, and treatment can be challenging. This case report presents a 43-year-old man with persistent parvovirus B19 infection and anaemia. The patient showed resistance to standard treatments but responded well to pegylated interferon alfa-2a. This highlights the potential of interferon alfa-2a as a treatment option for persistent parvovirus B19 infection.
LANCET INFECTIOUS DISEASES
(2023)
Article
Medicine, General & Internal
Qihang Zou, Peisong Chen, Jiaxin Chen, Dingbang Chen, Han Xia, Ling Chen, Huiyu Feng, Li Feng
Summary: This report presents a case of multisystem dysfunction caused by B19V infection in a non-immunosuppressed adult. The pathogen was successfully detected in multiple types of samples using mNGS technology and confirmed by PCR. Treatment with intravenous gamma globulin resulted in significant symptom resolution.
FRONTIERS IN MEDICINE
(2022)
Review
Microbiology
Liting Shao, Weiran Shen, Shengqi Wang, Jianming Qiu
Summary: Human bocavirus 1 (HBoV1) is an autonomous human parvovirus that causes acute respiratory tract infections in children and infects human airway epithelial cells in vitro. It expresses multiple proteins and RNA, playing important roles in viral gene expression and replication, and serving as a helper virus for other viruses in human cells.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Mengqing Yang, Jiangang Ma, Zhili Chu, Xuhong Cao, Kejia Lu, Xiaolei Shi, Lina Tong, Chuanqi Yan, Haijin Liu, Xinglong Wang, Sa Xiao, Zengqi Yang
Summary: The study revealed a potential interaction between MSI1 and NDV V protein, indicating that MSI1 may act as a new antiviral molecule by inhibiting viral release and blocking apoptosis to inhibit the release of NDV.
Article
Biochemistry & Molecular Biology
Ieva Rinkunaite, Egidijus Simoliunas, Daiva Bironaite, Rasa Rutkiene, Virginija Bukelskiene, Rolandas Meskys, Julius Bogomolovas
Summary: The unique region VP1u plays a crucial role in B19V's tropism for erythroid precursor cells, but its interaction with endothelial cells leads to upregulation of cellular stress signaling pathways, potentially causing endothelial damage during acute B19V infection.
Article
Psychology, Multidisciplinary
Ai-bao Zhou, Yue Yuan, Hai-yan Wang, Hai-yan Zhao, Jin-guo Zhao, Yi-tong Wang, Lu Cai
Summary: This study investigated the impact of attentional resources and emotional reactivity on emotional memory enhancement in adolescents and adults. The results showed that age and attentional resources had an interaction effect on emotional memory enhancement, with higher levels observed in adults compared to adolescents and in the sufficient attentional resources task. Negative emotions were significantly higher than positive and neutral emotions, and positive emotions were significantly higher than neutral emotions. Emotional reactivity and age also had an interaction effect on emotional memory enhancement, with adolescents and adults with high emotional reactivity showing significantly higher levels of negative emotions compared to positive and neutral emotions. Emotional memory enhancement was moderated by attentional resources during the encoding process and emotional reactivity.
CURRENT PSYCHOLOGY
(2023)
Article
Materials Science, Ceramics
Adam Bratten, Visharad Jalan, Meng Shi, Tyler Gerczak, Haiming Wen, Peter Doyle, Haiyan Zhao, Xiaoqing He
Summary: Surrogate tristructural-isotropic (TRISO)-coated fuel particles were oxidized in 0.2 kPa O-2 to examine the behavior of the SiC layer. The analysis of the resulting SiO2 layers showed a majority of smooth, amorphous SiO2 with a constant thickness indicative of passive oxidation. The activation energy for oxide growth was consistent across all temperatures and the presence of irregular grain size influenced the oxidation response of the SiC layer.
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Faqi Zhan, Haiyan Zhao, Guochang Wen, Zhiqing Ma, Yisi Liu, Min Zhu, Yuehong Zheng, Dalin Chen, Peiqing La
Summary: A novel ternary NiPx/NiWO4/WO3 photoanode with a heterojunction and cocatalyst was fabricated by an in situ phosphatization process. The heterojunction between NiWO4 and WO3 improved electron-hole pair separation and transfer, while NiPx acted as a cocatalyst to accelerate the surface water oxidation reaction. Compared to the pristine WO3 film, the photocurrent density at 1.23 V vs. RHE was increased by 2.55-fold.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Environmental Sciences
Haiyan Zhao, Ziqian Wang, Yonghong Liang, Tianxiang Wu, Yiliang Chen, Jieru Yan, Yiyong Zhu, Dahu Ding
Summary: Efficient adsorption of antibiotics from livestock wastewater is urgently needed. In this study, alkaline-modified biochar with larger surface area and pore volume was used for the adsorption of different types of antibiotics. The adsorption process was mainly determined by chemisorption and was influenced by solution pH. The study also revealed that the -OH groups on biochar surface were the dominant active sites for antibiotics adsorption. Furthermore, the biochar showed synergistic adsorption towards Zn2+/Cu2+ and antibiotics in a multi-pollutants system.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Dan Zhao, Xiaoyun Li, Mengyu Xu, Yan Jiao, Huan Liu, Xincai Xiao, Haiyan Zhao
Summary: This paper reports the preparation of yellow-green-fluorescent and high-quantum yield carbon dots (4-ACDs) and investigates their optical properties. The 4-ACDs show excellent broad-spectrum antibacterial activity and biocompatibility, making them ideal for cell imaging. The coupling of 4-ACDs and lysozyme (LZM) results in CDs-LZM complex with strong antibacterial activity against Gram-positive bacteria and efficient elimination of biofilm.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Tianjia Sun, Yingquan Zhang, Qingli Yang, Jinfu Ban, Boli Guo, Bo Zhang, Haiyan Zhao
Summary: The study aimed to investigate the spatio-temporal variation of arabinoxylan (AX) and alkylresorcinol (AR) in wheat. The contents of total arabinoxylan (TOT-AX), water-extractable arabinoxylan (WE-AX), and alkylresorcinol (AR) in wheat from the main wheat producing areas of China were analyzed. Factors such as geographical origin, variety, harvest year, and irrigation frequency were found to significantly affect the AX and AR contents in wheat grains. The study identified Xiaoyan 22, Xinmai 18, and Hengguan 35 as the varieties with the highest TOT-AX, WE-AX, and AR contents, respectively.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Physical
Fan Yang, Jiayun Pei, Haiyan Zhao
Summary: This study investigates ultrathin carbon-deficient molybdenum carbide (MoC0.66) as a catalyst for the capture and conversion of carbon dioxide (CO2) to methane (CH4). The results show that MoC(0.66) exhibits remarkable catalytic activity for CO2 hydrogenation to CH4. Unlike conventional catalysts, the limiting step of the MoC0.66 catalyst is determined by the release of *OH species during the CO2 reduction reaction (CO2RR). Increasing the temperature improves the release of H2O and CH4 as well as the selectivity of the CO2RR.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Biochemistry & Molecular Biology
Na Li, Hongyue Zhang, Min Cui, Jujie Ren, Jingru Huang, Bao Sun, Haiyan Zhao, Cong Zhang
Summary: The preparation of covalent polyoxometalate organic frameworks (CPOFs) was introduced using polyoxometalate and covalent organic frameworks. The CPOFs were synthesized by functionalizing polyoxometalate with an amine group and then reacting it with 2,4,6-trihydroxybenzene-1,3,5-tricarbaldehyde. The PtNPs-CPOFs-MWCNTs nanocomposites were formed by incorporating PtNPs and MWCNTs into the CPOFs material, resulting in excellent catalytic activity and electrical conductivity for the electrochemical thymol sensors.
Article
Engineering, Marine
Lepeng Chen, Rongxin Cui, Weisheng Yan, Hui Xu, Haiyan Zhao, Haoquan Li
Summary: Underwater robot has potential to clean ship hull fouling, but design and control are challenging. Use of negative pressure and magnetic adsorption technologies limit utility on rough hulls and warships. We propose a robot with thrusters and crawler belts to adapt to common hulls. A climbing controller guides robot to cover hull without repetition or omission, overcoming difficulties of inconsistent crawler belts and inaccurate depth measurement. Field experiments on giant ship verify effectiveness of proposed solution and controller.
Article
Chemistry, Analytical
Shuang Lin, Xiaoyu Fang, Guoqiang Fang, Fengping Liu, Haoyu Dong, Haiyan Zhao, Jing Zhang, Bin Dong
Summary: In this study, a 3D clean cascade-enhanced nanosensor assisted by machine learning algorithms was developed for accurate and ultrasensitive identification of multiple pollutants. The SERS substrate achieved cascading electromagnetic energy with a remarkable enhancement factor of 8.35 x 109, thanks to the combination of micro-level polystyrene sphere porous array and nano-level Au-Ag clusters. With high cleanliness and ultra-sensitivity, hazardous pollutants with similar geometry and Raman peaks at ultra-low concentration were successfully distinguished using principal component analysis. Therefore, this efficient and clean SERS substrate, coupled with artificial intelligence, could advance the application of SERS technology in accurate identification of trace contaminants.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Genetics & Heredity
Xiao-han Chen, Meng Leng, Di-hui Yao, Hai-yan Zhao, Mao-fa Yang
Summary: In this study, we confirmed that the complete mitochondrial genome of Picromerus lewisi Scott has similar characteristics to other Hemiptera species. The mitogenome of P. lewisi is 18,123 bp long with 74.0% A + T content and contains 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs, and one control region. Phylogenetic analysis based on 13 PCGs from 17 Panheteroptera species revealed that P. lewisi is closely related to E. thomsoni within the Pentatomidae family.
MITOCHONDRIAL DNA PART B-RESOURCES
(2023)
Article
Environmental Sciences
Jiayu Dong, Xianni Song, Shuchi Zhang, Mengyu Tan, Haiyan Zhao, Donglei Wu
Summary: A novel hierarchical Cu/ZSM-5 catalyst with large pore volume and size was prepared for the catalytic ozonation of PVA. The catalyst exhibited excellent mineralization performance, with a TOC removal rate of 47.86% after 60 minutes of reaction. O-1(2) played a more significant role than ·OH in PVA removal. The catalyst showed high catalytic activity and stability, making it promising for the treatment of refractory pollutants via catalytic ozonation.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Physical
Muhammad Qasim Zafar, Jinnan Wang, Zhenlin Zhang, Chaochao Wu, Haiyan Zhao, Ghulam Hussain, Ninshu Ma
Summary: Laser cladding is a promising technique for fabrications, remanufacturing, and repair of metallic components. Residual stresses caused by melting and solidification in the process often lead to geometric discrepancies and deterioration of the end product. A hybrid FEM scheme is adopted to efficiently predict the temperature field, residual stress, and distortion in multilayer powder-fed laser cladding. The accuracy of the simulation is validated through experiments, showing precise agreement for residual stress and distortion measurements.
Article
Radiology, Nuclear Medicine & Medical Imaging
Yun Bian, Jian Zhou, Mengmeng Zhu, Jieyu Yu, Haiyan Zhao, Xu Fang, Fang Liu, Tiegong Wang, Jing Li, Li Wang, Jianping Lu, Chengwei Shao
Summary: This study aimed to develop and validate a radiomics nomogram based on a fully automated pancreas segmentation for the assessment of pancreatic exocrine function. The performance of the radiomics nomogram was compared with the pancreatic flow output rate (PFR) and conclusions were drawn on the replacement of secretin-enhanced magnetic resonance cholangiopancreatography (S-MRCP) by the radiomics nomogram for pancreatic exocrine function assessment.
EUROPEAN RADIOLOGY
(2023)
Article
Virology
Siyuan Hao, Xiujuan Zhang, Kang Ning, Zehua Feng, Soo Yeun Park, Cagla Aksu Kuz, Shane McFarlin, Donovan Richart, Fang Cheng, Elizabeth Yan Zhang, Aaron Zhang-Chen, Ziying Yan, Jianming Qiu
Summary: rAAV2.5T, selected through directed evolution, is capable of efficiently transducing human airway epithelium. Our study revealed that KIAA0319L is not essential for vector internalization but plays a critical role in efficient vector transduction to human airway epithelium. We also identified a novel gene, WDR63, which is important for vector transduction of human airway epithelium but not for vector internalization and nuclear entry. Additionally, our study discovered the significant transduction potential of rAAV2.5T in the basal stem cells of human airway epithelium, highlighting its utility in gene editing for pulmonary genetic diseases.
JOURNAL OF VIROLOGY
(2023)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
