Journal
VIROLOGY
Volume 468, Issue -, Pages 226-237Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2014.07.043
Keywords
Minute virus of mice (MVM); Human bocavirus 1 (HBoV1); Parvovirus; Non-structural proteins; NS2; NP1; Functional complementation; DNA replication block
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Funding
- Public Health Service Grants from National Institutes of Health [CA029303, AI026109]
- CTSA from National Center for Advancing Translational Sciences [UL1 TR000142]
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Parvoviruses encode a small number of ancillary proteins that differ substantially between genera. Within the genus Protoparvovirus, minute virus of mice (MVM) encodes three isoforms of its ancillary protein NS2, while human bocavirus 1 (HBoV1), in the genus Bocaparvovirus, encodes an NP1 protein that is unrelated in primary sequence to MVM NS2. To search for functional overlap between NS2 and NP1, we generated murine A9 cell populations that inducibly express HBoV1 NP1. These were used to test whether NP1 expression could complement specific defects resulting from depletion of MVM NS2 isoforms. NP1 induction had little impact on cell viability or cell cycle progression in uninfected cells, and was unable to complement late defects in MVM virion production associated with low NS2 levels. However, NP1 did relocate to MVM replication centers, and supports both the normal expansion of these foci and overcomes the early paralysis of DNA replication in NS2-null infections. (C) 2014 Elsevier Inc. All rights reserved.
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