Journal
VIROLOGY
Volume 423, Issue 2, Pages 143-151Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2011.12.003
Keywords
Vaccinia virus; Dissemination, spread from cell to cell; Casein kinase 2; Phosphorylation; Actin; Arp2/3; N-WASP; Src; RNAi; TBB; Listeria
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Funding
- PEW Latin American Fellows Program in the Biomedical Sciences
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Casein kinase 2 (CK2) is a pleiotropic serine/threonine kinase that regulates numerous cellular processes and is essential to the infectious cycle of several viruses. Here we investigated the potential role of CK2 in vaccinia virus (VACV) infection. We used the CK2 inhibitor TBB and found that CK2 inactivation impaired VACV dissemination and actin tail formation. We used RNAi and confirmed that CK2 depletion impaired VACV actin tail formation. Furthermore, we designed a recombinant virus that allowed us to specifically detect cell-associated enveloped viruses (CEVs) at the plasma membrane and demonstrated that CK2 inactivation does not affect CEV formation. Finally, we showed that CK2 depletion impaired the recruitment of Src to CEVs. We discuss the possibility that CK2 may stimulate the A36-dependent recruitment of Src through A36 phosphorylation. (C) 2011 Elsevier Inc. All rights reserved.
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