Journal
VIROLOGY
Volume 412, Issue 1, Pages 179-187Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2010.12.057
Keywords
Novel DNA virus; Japanese eel endothelial cells-infecting virus; Anguilla japonica
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [21580235]
- Grants-in-Aid for Scientific Research [21580235] Funding Source: KAKEN
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Economic loss due to viral endothelial cell necrosis of eel (VECNE) of Anguilla japonica is a serious problem for the cultured Japanese eel market. However, the viral genome responsible for VECNE is unknown. We recently developed a rapid determination system for viral nucleic acid sequences (RDV) to determine viral genome sequences. In this study, viral DNA fragments were obtained using RDV, and approximately 15-kbp circular full genome sequences were determined using a next-generation sequencing system, overlapping PCR, and Southern blot analysis. One open reading frame (ORF) was homologous to the large T-antigen of polyomavirus; other ORFs have no homology with any nucleic or amino acid sequences of polyomavirus. Therefore, as this DNA virus might comprise a novel virus family, we provisionally named it Japanese eel endothelial cells-infecting virus (JEECV). JEECV was detected in both naturally and experimentally infected eels, suggesting that JEECV potentially causes VECNE. (c) 2011 Elsevier Inc. All rights reserved.
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