Article
Biochemistry & Molecular Biology
Aditya Mittal, Anandkumar Madhavjibhai Changani, Sakshi Taparia
Summary: This study identified unique signatures of intrinsic disorder by systematically synthesizing different peptide libraries, expanding the known intrinsically disordered proteins and providing a major advancement in exploring the functional manifestations of such proteins.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Review
Biochemistry & Molecular Biology
Tanuj Handa, Debanjan Kundu, Vikash Kumar Dubey
Summary: The importance of protein structure in performing biological functions optimally is being challenged by the emergence of intrinsically disordered proteins (IDPs) and intrinsically disordered protein regions (IDPRs). Understanding the evolutionary and functional significance of these proteins is crucial in studying various organisms. This review highlights the importance of IDPs in the physiology of specific organisms and establishes a positive correlation between IDPs and their relevance in human health and future medicine. Extensive research on IDPs and IDPRs will greatly contribute to expanding the field of structural biology, aiding in the comprehension of protein folding, misfolding, associated diseases, and drug design.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Myriam Guillien, Assia Mouhand, Aurelie Fournet, Amandine Gontier, Aleix Marti Navia, Tiago N. Cordeiro, Frederic Allemand, Aurelien Thureau, Jean-Louis Baneres, Pau Bernado, Nathalie Sibille
Summary: This study used an integrated biophysical strategy to describe the basal conformations of the C-terminal domains of three class A GPCRs, and revealed the presence of transient secondary structures in these regions that may be involved in interaction with arrestin. The findings provide insights into the molecular mechanisms of GPCR signal transduction.
Review
Biochemistry & Molecular Biology
Toshio Ando
Summary: High-speed atomic force microscopy (HS-AFM) can directly visualize the dynamic structure of intrinsically disordered proteins (IDPs) and reveal their unique functions. This technique does not alter the dynamic structure of IDPs and can estimate the number of amino acids in fully disordered regions from images. In addition, the dumbbell-like structure observed on IDPs has important functional relevance.
Review
Biochemistry & Molecular Biology
Emma Langella, Martina Buonanno, Giuseppina De Simone, Simona Maria Monti
Summary: hCA IX is a tumor-associated enzyme that is over-expressed in many tumors. This article aims to provide an overview of the poorly investigated proteoglycan-like domain of hCA IX and its role in modulating catalytic activity, tumor spreading, and migration. Special attention is given to the newly discovered disordered features of the PG domain and its potential diagnostic and therapeutic applications.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Virology
Viktoria Kolbe, Wing H. Ip, Lisa Kieweg-Thompson, Judith Lang, Julia Gruhne, Tina Meyer, Britta Wilkens, Marcel Schie, Roland Thunauer, Sabrina Schreiner, Luca D. Bertzbach, Estefania Rodriguez, Thomas Dobner
Summary: This research identified potential SUMO-conjugation motifs in E1B-55K proteins from HAdV species A to F and demonstrated that these proteins are SUMOylated at a highly conserved lysine residue. The study also revealed that E1B-55K SUMOylation regulates intracellular localization and p53-mediated transcription in most HAdV species.
JOURNAL OF VIROLOGY
(2022)
Review
Biochemistry & Molecular Biology
Samuel Naudi-Fabra, Martin Blackledge, Sigrid Milles
Summary: Single molecule fluorescence and nuclear magnetic resonance spectroscopy are powerful techniques for analyzing intrinsically disordered proteins. They provide complementary views to decipher the complex properties and interactions of IDPs, and have made significant contributions to our understanding of their molecular characteristics.
Review
Biochemistry & Molecular Biology
Federica Quaglia, Edoardo Salladini, Marco Carraro, Giovanni Minervini, Silvio C. E. Tosatto, Philippe Le Mercier
Summary: The presence of intrinsically disordered regions (IDRs) in SARS-CoV-2 viral proteins, particularly in the S1 subunit of spike and nucleoprotein, plays a crucial role in the emergence of successive variants and antigenicity. Mutations occurring in these IDRs are critical for immune evasion and antibody escape. This suggests the importance of understanding the structural flexibility of viral proteins for vaccine and therapeutic strategies.
Review
Biochemistry & Molecular Biology
Birgit Strodel
Summary: Although AlphaFold has made breakthroughs in protein structure prediction, it cannot predict protein folding pathways and structures of intrinsically disordered proteins. Molecular dynamics simulations offer an alternative computational method and have successfully explored the energy landscapes of IDP conformational switching and protein aggregation.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Milos Avramov, Eva Schad, Agnes Revesz, Lilla Turiak, Iva Uzelac, Agnes Tantos, Laszlo Drahos, Zeljko D. Popovic
Summary: This study utilizes wet lab methods and bioinformatics tools to analyze intrinsically disordered proteins in a cold-hardy insect species. The results suggest that these proteins play important roles in the insect's adaptive mechanisms to extreme temperatures.
Review
Biochemistry & Molecular Biology
Rakesh Trivedi, Hampapathalu Adimurthy Nagarajaram
Summary: This review discusses different aspects of disordered proteins and protein regions, as well as the experimental and computational methods used to characterize them. Additionally, the role of disordered proteins in diseases and their potential as drug targets are explored.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Guy Jacoby, Merav Segal Asher, Tamara Ehm, Inbal Abutbul Ionita, Hila Shinar, Salome Azoulay-Ginsburg, Ido Zemach, Gil Koren, Dganit Danino, Michael M. Kozlov, Roey J. Amir, Roy Beck
Summary: This study introduces a new type of peptide amphiphiles, intrinsically disordered peptide amphiphiles (IDPA), that exhibit a sharp pH-induced micellar phase-transition. The shape transition can serve as a mechanism for the design of cargo hold-and-release applications, demonstrating the potential of tailoring interactions between disordered peptides for various stimuli-responsive biomedical applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Biochemistry & Molecular Biology
Kiersten M. Ruff, Rohit Pappu
Summary: Accurate predictions of protein structures using AlphaFold have made significant progress. Most protein sequences in the human proteome have been structurally annotated, with over 30% of regions being disordered.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chengtao Ding, Sheng Wang, Zhiyong Zhang
Summary: IDPs are crucial in biological functions but difficult to study due to lack of stable structures. This study uses computational methods and experimental data to efficiently sample UL11 conformations, providing a general protocol for studying IDPs' structural ensembles.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lazar M. Vasovic, Gordana M. Pavlovic-Lazetic, Jovana J. Kovacevic, Milos V. Beljanski, Vladimir N. Uversky
Summary: This paper discusses the properties of proteins and their relationships in the interactomes of SARS-CoV-2, focusing on protein disorder and node degrees in interaction networks. By combining viral interactomes with the interactome of human lung tissue, it is found that new connections in the viral-host interactome are linked to protein disorder.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Roland Frach, Patrick Kibies, Saraphina Boettcher, Tim Pongratz, Steven Strohfeldt, Simon Kurrmann, Joerg Koehler, Martin Hofmann, Werner Kremer, Hans Robert Kalbitzer, Oliver Reiser, Dominik Horinek, Stefan M. Kast
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2016)
Article
Biochemistry & Molecular Biology
Michael Spoerner, Matthias Karl, Pedro Lopes, Marcus Hoering, Karoline Loeffel, Andrea Nuehs, Joseph Adelsberger, Werner Kremer, Hans Robert Kalbitzer
JOURNAL OF BIOMOLECULAR NMR
(2017)
Article
Chemistry, Physical
Paul Hendrik Schummel, Andreas Haag, Werner Kremer, Hans Robert Kalbitzer, Roland Winter
JOURNAL OF PHYSICAL CHEMISTRY B
(2016)
Article
Biochemistry & Molecular Biology
Silvia De Sanctis, Michael Wenzler, Nils Kroeger, Wilhelm M. Malloni, Manfred Sumper, Rainer Deutzmann, Patrick Zadravec, Eike Brunner, Werner Kremer, Hans Robert Kalbitzer
Article
Multidisciplinary Sciences
Sunilkumar Puthenpurackal Narayanan, Divya Gopalakrishnan Nair, Daniel Schaal, Marisa Barbosa de Aguiar, Sabine Wenzel, Werner Kremer, Stephan Schwarzinger, Hans Robert Kalbitzer
SCIENTIFIC REPORTS
(2016)
Article
Chemistry, Multidisciplinary
Markus Beck Erlach, Hans Robert Kalbitzer, Roland Winter, Werner Kremer
Article
Biochemistry & Molecular Biology
Tobias Harsch, Philipp Schneider, Barbel Kieninger, Harald Donaubauer, Hans Robert Kalbitzer
JOURNAL OF BIOMOLECULAR NMR
(2017)
Article
Biochemistry & Molecular Biology
Markus Beck Erlach, Joerg Koehler, Edson Crusca, Claudia E. Munte, Masatsune Kainosho, Werner Kremer, Hans Robert Kalbitzer
JOURNAL OF BIOMOLECULAR NMR
(2017)
Article
Chemistry, Multidisciplinary
Italo A. Cavini, Claudia E. Munte, Markus Beck Erlach, Thomas van Groen, Inga Kadish, Tao Zhang, Tamar Ziehm, Luitgard Nagel-Steger, Janine Kutzsche, Werner Kremer, Dieter Willbold, Hans Robert Kalbitzer
CHEMICAL COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Daniela Baumstark, Werner Kremer, Alfred Boettcher, Christina Schreier, Paul Sander, Gerd Schmitz, Renate Kirchhoefer, Fritz Huber, Hans Robert Kalbitzer
JOURNAL OF LIPID RESEARCH
(2019)
Article
Biophysics
Raphael Kreitner, Claudia E. Munte, Katrin Singer, Karl O. Stetter, Gudrun Horn, Werner Kremer, Hans Robert Kalbitzer
BIOMOLECULAR NMR ASSIGNMENTS
(2020)
Article
Biochemistry & Molecular Biology
Markus Beck Erlach, Joerg Koehler, Claudia E. Munte, Werner Kremer, Edson Crusca, Masatsune Kainosho, Hans Robert Kalbitzer
JOURNAL OF BIOMOLECULAR NMR
(2020)
Article
Chemistry, Multidisciplinary
Eric Girard, Pedro Lopes, Michael Spoerner, Anne-Claire Dhaussy, Thierry Prange, Hans Robert Kalbitzer, Nathalie Colloc'h
Summary: In this study, the researchers used high pressure crystallography to investigate the allosteric transitions of Ras protein. They found that pressure can shift the equilibria between different conformational states, providing insight into the structural plasticity involved in Ras protein's allosteric equilibria.
Article
Biochemistry & Molecular Biology
Konstanze von Koenig, Norman Kachel, Hans Robert Kalbitzer, Werner Kremer
Article
Biochemistry & Molecular Biology
Matthias Karl, Michael Spoerner, Thuy-Vy Pham, Sunilkumar Puthenpurackal Narayanan, Werner Kremer, Hans Robert Kalbitzer
BIOPHYSICAL CHEMISTRY
(2017)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.