Journal
VIROLOGY
Volume 397, Issue 1, Pages 205-216Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2009.11.020
Keywords
Human papillomavirus; CUT primers; Novel types; Phylogeny; HPV 115; Complete genome; South America
Categories
Funding
- Agencia Nacional de Prornocion Cientifica y Tecnologica [PICT 05-25464]
- Fogarty International Center/NIH [D43 TW0010137]
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We explored the cutaneotropic HPV genetic diversity in 71 subjects from Argentina. New generic primers (CUT) targeting 88 mucosal/cutaneous HPV were designed and compared to FAP primers. Overall, 69 different HPV types/putative types were identified, being 17 of them novel putative types. Phylogenetic analysis of partial L1 sequences grouped 2 novel putative types in the Beta-PV, 14 in the Gamma-PV and I in the Mu-PV genera. CUT primers showed broader capacity than FAP primers in detecting different genera/species and novel Putative types (p<0.01). Using overlapping PCR, the full-length genome of a Beta-PV putative type was amplified and cloned. The new virus, designated HPV 115, encodes five early genes and two late genes. Phylogenetic analysis indicated HPV 115 as the most divergent type within the genus Beta-PV species 3. This report is the first providing data on cutaneous HPVs circulating in South America and expands our knowledge of the Papillomaviridae family. (C) 2009 Elsevier Inc. All rights reserved.
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