Journal
VIROLOGY
Volume 378, Issue 2, Pages 355-362Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2008.06.003
Keywords
equine arteritis virus; reverse genetics; infectious clone; structural and nonstructural proteins; attenuation; vaccine
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Funding
- Fort Dodge Animal Health
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Comparative sequence analysis of a series of strains of equine arteritis Virus (EAV) of defined Virulence for horses, ranging from the horse-adapted Virulent Bucyrus (VB) strain to a fully attenuated vaccine strain derived from it, identified 13 amino acid substitutions associated with attenuation. These include 4 substitutions ill the replicase proteins and 9 in the Structural proteins. Using reverse genetic techniqUeS, these amino acid Substitutions Were introduced into a virulent infectious cDNA clone pEAVrVBS derived from the VB strain of EAV. Inoculation of horses with the recombinant Viruses clearly demonstrated that changes ill either the replicase (nsp1, nsp2 and nsp7) Or structural proteins (GP2, GP4, GP5 and M) resulted in attenuation of the virulent VB strain. The recombinant Virus with substitutions ill the structural proteins was more attenuated than the recombinant virus with substitutions only in the replicase proteins. (C) 2008 Elsevier Inc. All rights reserved.
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