Article
Virology
Kosuke Oda, Yasuyuki Matoba, Masanori Sugiyama, Takemasa Sakaguchi
Summary: The study reveals the crucial role of the binding between the Respirovirus C protein and Alix in viral replication, with mutant C proteins of varying binding affinities showing impaired virus production. This interaction is essential for viral budding and provides insights for the development of new antiviral drugs against hPIV1.
JOURNAL OF VIROLOGY
(2021)
Review
Multidisciplinary Sciences
Jessica Sook Yuin Ho, Zeyu Zhu, Ivan Marazzi
Summary: Viruses have evolved unique strategies for gene expression by altering conventional gene-protein production line steps with dedicated viral enzymes or host factors. Targeting these unique enzymatic activities and host factors can expose vulnerabilities of a virus, providing a paradigm for novel antiviral therapies. Understanding the mechanisms of unconventional gene and protein expression in viruses could guide translational efforts aimed at viral eradication.
Review
Virology
Daniel Kolakofsky, Philippe Le Mercier, Machiko Nishio, Martin Blackledge, Thibaut Crepin, Rob W. H. Ruigrok
Summary: VSV and SeV are members of the Mononegavirales order, studying these viruses can provide insights into their genetic information expression and RNA synthesis processes, as well as the characteristics of their nucleocapsid, helping to establish a unified model based on commonalities among these viruses.
Article
Cell Biology
Linjuan Wu, Dongning Jin, Dan Wang, Xuping Jing, Peng Gong, Yali Qin, Mingzhou Chen
Summary: A study found that the N-terminus of the Ebola virus nucleoprotein contributes to the release of virus-like particles (VLPs) containing nucleoprotein by recruiting the matrix protein VP40. Mutations in the C-terminal hydrophobic core of nucleoprotein strengthen the interaction with VP40, leading to reduced VLP release and inhibition of viral RNA synthesis. This suggests that the interaction between the N-terminus of nucleoprotein and VP40 induces a conformational change in the C-terminus of nucleoprotein, inhibiting RNA synthesis and initiation of virion assembly/budding.
Article
Microbiology
Naoko Morita, Yukie Tanaka, Kenji Takeuchi, Yoshinori Kitagawa, Ryusuke Sakuma, Naoki Koide, Takayuki Komatsu
Summary: Macrophages play a crucial role in the immune response against respiratory viral infections. However, viral pathogens have developed virulence factors to counteract macrophage function. The accessory protein C from Sendai virus (SeV) can inhibit the secretion of pro-inflammatory factors and lead to morphological changes in macrophages. This study suggests that the C protein may limit phagocytosis in SeV-infected macrophages by suppressing membrane ruffling.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Muhammad Ikram Anwar, Ni Li, Qing Zhou, Mingxiao Chen, Chengguang Hu, Tao Wu, Haihang Chen, Yi-Ping Li, Yuanping Zhou
Summary: This study identified that PPP2R5D interacts with HCV NS5B and is essential for HCV infection in cultured hepatoma cells by facilitating HCV replication.
Article
Cell Biology
Nicolas Fossat, Emma A. Lundsgaard, Rui Costa, Lizandro R. Rivera-Rangel, Louise Nielsen, Lotte S. Mikkelsen, Santseharay Ramirez, Jens Bukh, Troels K. H. Scheel
Summary: We used CLEAR-CLIP to investigate the interaction between SARS-CoV-2 and miRNA, revealing the regulation of cellular miRNA and the interaction with miR-15 family during infection. We found that these interactions are not critical for virus production. This work contributes to understanding the interplay between miRNA regulation and SARS-CoV-2 infection.
Article
Biochemistry & Molecular Biology
Monica L. Husby, Souad Amiar, Laura Prugar, Emily A. David, Caroline B. Plescia, Kathleen E. Huie, Jennifer M. Brannan, John M. Dye, Elsje Pienaar, Robert Stahelin
Summary: Phosphatidylserine (PS) plays a crucial role in the assembly and spread of lipid-enveloped viruses. The Ebola virus matrix protein eVP40 induces clustering of PS and promotes viral budding in vitro, while the FDA-approved drug fendiline reduces PS clustering and inhibits virus budding and entry. Fendiline decreases PS content in mammalian cells and the plasma membrane, leading to a significant decrease in the ability of VP40 to form new virus particles. Moreover, particles formed from fendiline-treated cells have altered morphology and cannot efficiently infect or enter cells. These findings provide insights into the mechanism by which PS clustering enhances viral assembly, budding, and spread, and lay the foundation for potential drug targeting strategies.
Article
Microbiology
Haiying Mao, Lei Cao, Ting Xu, Xiaohan Xia, Peilei Ren, Pengfei Han, Chengfei Li, Xianfeng Hui, Xian Lin, Kun Huang, Meilin Jin
Summary: This study identified a novel host factor, YWHAG, that interacts with the IAV matrix protein M2 and regulates viral budding. Knockout of YWHAG increased viral production, while overexpression reduced the titer of virus progeny.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Cell Biology
Kate Dicker, Aino I. Jarvelin, Manuel Garcia-Moreno, Alfredo Castello
Summary: RNA viruses rely on cellular RNA-binding proteins to facilitate replication and spread, with a pool of RBPs typically incorporated into viral particles. These RBPs play crucial roles in viral particle formation and infectivity, suggesting broader implications for host RBPs in virus infection than previously thought.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Mei-Lin Yang, Yi-Cheng Chen, Chung-Teng Wang, Hao-Earn Chong, Nai-Hui Chung, Chia-Hsing Leu, Fu-Tong Liu, Michael M. C. Lai, Pin Ling, Chao-Liang Wu, Ai-Li Shiau
Summary: Galectin-3 enhances viral infection by increasing vRNP nuclear import and RdRp activity, thereby facilitating viral transcription and replication. This study identifies galectin-3 as a potential therapeutic target for influenza.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Biochemical Research Methods
Jason Yeung, Andrew L. Routh
Summary: In this study, we present ViReMaShiny, a web-based application built using the R Shiny framework, which allows interactive exploration and point-and-click visualization of viral recombination data in BED format. This tool can help researchers contextualize recombination events and perform downstream analysis.
Article
Virology
Oliver Siering, Bevan Sawatsky, Christian K. Pfaller
Summary: Paramyxoviruses, such as measles virus (MeV) and canine distemper virus (CDV), express accessory proteins like the C protein, which play important roles in regulating the host's immune response and viral replication. MeV C protein prevents excessive production of double-stranded RNA, while a similar disruption in CDV's C open reading frame does not lead to attenuation. By introducing additional mutations to prevent expression of truncated C proteins, a new CDV was successfully attenuated both in cell culture and in ferrets, revealing the crucial role of morbillivirus C proteins in pathogenesis.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Edward Dobbs, Greg Deakin, Julie Bennett, Caoimhe Fleming-Archibald, Ian Jones, Helen Grogan, Keny Burton
Summary: The study examined viral interactions in Agaricus bisporus cultures with 9 viruses, identifying four clusters of viral RNA indicating synergism and coreplication. Diseased fruitbodies showed significantly higher levels of specific viral RNA, suggesting an exponential shift in replicative capacity. These interactions appeared to be stochastic, with disease symptoms randomly distributed and high levels of specific viral RNA present.
Article
Microbiology
De-Sheng Ker, Huw T. Jenkins, Sandra J. Greive, Alfred A. Antson
Summary: The study reports the first cryoEM structure of a Henipavirus RNA-bound nucleocapsid assembly, revealing the composition of its helical assembly and the wrapping of RNA. The structure shows similarities and differences with other members of the Paramyxoviridae family and the evolutionarily distant Filoviridae Ebola virus.
Article
Biotechnology & Applied Microbiology
Makoto Hirayama, Hiromi Shibata, Koji Imamura, Takemasa Sakaguchi, Kanji Hori
MARINE BIOTECHNOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Makoto Hirayama, Hiromi Shibata, Koji Imamura, Takemasa Sakaguchi, Kanji Hori
MARINE BIOTECHNOLOGY
(2016)
Correction
Biotechnology & Applied Microbiology
Makoto Hirayama, Hiromi Shibata, Koji Imamura, Takemasa Sakaguchi, Kanji Hori
MARINE BIOTECHNOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Kosuke Oda, Takashi Oda, Yasuyuki Matoba, Mamoru Sato, Takashi Irie, Takemasa Sakaguchi
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Virology
Asuka Yoshida, Ryoko Kawabata, Tomoyuki Honda, Kouji Sakai, Yasushi Ami, Takemasa Sakaguchi, Takashi Irie
JOURNAL OF VIROLOGY
(2018)
Article
Engineering, Biomedical
Kentaro Sueoka, Taiichiro Chikama, Miftahul Akhyar Latief, Ji-Ae Ko, Yoshiaki Kiuchi, Takemasa Sakaguchi, Akira Obana
LASERS IN MEDICAL SCIENCE
(2018)
Article
Multidisciplinary Sciences
Yuta Tsukamoto, Sotaro Ikeda, Koji Uwai, Riho Taguchi, Kazuaki Chayama, Takemasa Sakaguchi, Ryo Narita, Wan-Ling Yao, Fumihiko Takeuchi, Yukie Otakaki, Koichi Watashi, Takaji Wakita, Hiroki Kato, Takashi Fujita
Article
Biology
Takahito Ohmine, Seika Narai, Toshiki Matsubara, Toshihito Nomura, Kosuke Oda, Masaya Fukushi, Takashi Irie, Takayuki Komatsu, Yukinobu Tohya, Takemasa Sakaguchi
BIOCONTROL SCIENCE
(2018)
Article
Orthopedics
Muhammad P. Johan, Tadahiko Kubo, Taisuke Furuta, Tomohiko Sakuda, Takemasa Sakaguchi, Mahito Nakanishi, Mitsuo Ochi, Nobuo Adachi
JOURNAL OF ORTHOPAEDIC RESEARCH
(2018)
Article
Multidisciplinary Sciences
Takayoshi Kawahara, Isamu Akiba, Megumi Sakou, Takemasa Sakaguchi, Hatsumi Taniguchi
Meeting Abstract
Ophthalmology
Yunialthy Dwia Pertiwi, Taiichiro Chikama, Kentaro Sueoka, Ji-Ae Ko, Yoshiaki Kiuchi, Hajime Onodera, Takemasa Sakaguchi
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2018)
Meeting Abstract
Ophthalmology
Taiichiro Chikama, Kentaro Sueoka, Ji-Ae Ko, Takemasa Sakaguchi, Yoshiaki Kiuchi
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2018)
Meeting Abstract
Ophthalmology
Taiichiro Chikama, Kentaro Sueoka, Ji-Ae Ko, Yoshiaki Kiuchi, Takemasa Sakaguchi, Akira Obana
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2017)
Article
Biochemical Research Methods
Kei Takenaka, Shigenori Togashi, Ryo Miyake, Takemasa Sakaguchi, Michihiro Hide
JOURNAL OF BREATH RESEARCH
(2016)
Meeting Abstract
Ophthalmology
Kentaro Sueoka, Taiichiro Chikama, Miftahul Akhyar Latief, Ji-Ae Ko, Yoshiaki Kiuchi, Takemasa Sakaguchi, Akira Obana
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2016)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.