Article
Pathology
Chung-Ta Lee, Nan-Haw Chow, Yi-Lin Chen, Chung-Liang Ho, Yu-Min Yeh, Shao-Chieh Lin, Peng-Chan Lin, Bo-Wen Lin, Chien-An Chu, Hung-Wen Tsai, Jenq-Chang Lee
Summary: Microsatellite instability (MSI) in colorectal cancers (CRCs) is associated with mismatch repair system deficiencies, particularly due to methylation of MMR genes and BRAF mutations. Different patterns of dMMR were found to be associated with specific clinicopathological features, with BRAF V600E mutations predominantly occurring in certain types. The expression patterns of MMR proteins also showed distinct associations with tumor staging and age at diagnosis.
PATHOLOGY RESEARCH AND PRACTICE
(2021)
Article
Oncology
Ning Zhao, Yinghao Cao, Jia Yang, Hang Li, Ke Wu, Jiliang Wang, Tao Peng, Kailin Cai
Summary: The study found that serum tumor markers and clinicopathological characteristics were significantly associated with the status of KRAS and MMR. The combination of these two factors had a strong predictive power for KRAS mutations and dMMR among colorectal cancer patients.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medicine, General & Internal
Masanao Kaneko, Mitsuko Nakashima, Kiichi Sugiura, Natsuki Ishida, Satoshi Tamura, Shinya Tani, Mihoko Yamade, Yasushi Hamaya, Satoshi Osawa, Kyota Tatsuta, Kiyotaka Kurachi, Satoshi Baba, Yuji Iwashita, Tomio Arai, Haruhiko Sugimura, Masato Maekawa, Ken Sugimoto, Moriya Iwaizumi
Summary: This study aimed to understand the histopathological characteristics of colorectal cancers (CRC) that exhibit both deficient mismatch repair (dMMR) and BRAFV600E mutation. The findings showed that medullary carcinomas have unique characteristics of harboring the BRAFV600E mutation and expressing dMLH1.
Article
Oncology
Johannes Uhlig, Michael Cecchini, Amar Sheth, Stacey Stein, Jill Lacy, Hyun S. Kim
Summary: This study found distinct mutational behavior in stage IV colorectal cancer based on microsatellite and KRAS status, with KRAS mutation, MSI-H, and primary CRC sidedness independently affecting overall survival and interacting with different prognostic profiles. Generically classifying adenocarcinomas at different sites as CRC may underestimate this diversity.
JOURNAL OF THE NATIONAL COMPREHENSIVE CANCER NETWORK
(2021)
Article
Chemistry, Analytical
Raluca-Ioana Stefan-van Staden, Alexandru Adrian Bratei, Ruxandra-Maria Ilie-Mihai, Damaris-Cristina Gheorghe, Bianca Maria Tuchiu
Summary: Two stochastic microsensors based on inulins immobilization were designed for the recognition and analysis of MLH1, MSH2, MSH6, PMS2, and KRAS proteins. The sensors showed different working concentration ranges and limits of determination in different biological samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Multidisciplinary Sciences
Hyoun Wook Lee, Boram Song, Kyungneun Kim
Summary: The presence of a residual adenoma component in colorectal cancer is associated with a high frequency of KRAS mutation and indolent clinicopathological features. The discordance in the incidence of KRAS mutation between the adenoma and carcinoma components emphasizes the need to document the adenoma component in the pathology report and avoid including it in molecular testing samples.
Article
Oncology
Joana Lemos Garcia, Isadora Rosa, Sofia Saraiva, Ines Marques, Ricardo Fonseca, Pedro Lage, Ines Francisco, Patricia Silva, Bruno Filipe, Cristina Albuquerque, Isabel Claro
Summary: Recognition of hereditary colorectal cancer (CRC) syndrome is crucial, and Lynch Syndrome (LS) is the most frequent immunohistochemistry (IHC)-screening for mismatch repair proteins (MMR) deficiency in CRC. An unicentric cohort study was conducted in a central Oncological Hospital to assess its results. Routine immunohistochemistry screening for MMR protein deficiency in CRC patients under 70 years-old identified Lynch Syndrome patients that could be missed using restrictive criteria. These results highlight the importance of universal CRC screening for MMR protein status.
Article
Medical Laboratory Technology
Chengfeng Wang, Diling Pan
Summary: This study investigated the relationship between BRAF, KRAS, and PIK3CA mutations and clinicopathologic features and prognosis of colorectal cancer patients. The results showed that the mutant patterns of BRAF, KRAS, and PIK3CA were not associated with the general and clinicopathological features of patients. However, these mutation patterns could be used as independent prognostic factors for colorectal cancer.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Medicine, General & Internal
Razvan Catalin Popescu, Cristina Tocia, Costel Brinzan, Georgeta Camelia Cozaru, Mariana Deacu, Andrei Dumitru, Nicoleta Leopa, Anca Florentina Mitroi, Anca Nicolau, Eugen Dumitru
Summary: Colorectal cancer is a heterogeneous disease with multiple molecular features, and this study aimed to assess the frequency of microsatellite instability in colon cancer. Results showed that microsatellite instability was found in 16.3% of patients, with MSI-H tumors more common in females and those with a family history of colorectal cancer. Additionally, BRAF mutations were identified in 13.9% of patients and were more often present in MSI tumors.
Article
Pathology
Tomoyuki Nakajima, Takeshi Uehara, Mai Iwaya, Kazuyuki Matsuda, Megumi Wada, Tadanobu Nagaya, Takehito Ehara, Hiroyoshi Ota
Summary: Tumor budding (TB) is an important prognostic factor in colorectal carcinoma (CRC). Osteopontin (OPN) analysis in CRC stromal cells may have prognostic implications.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Article
Cell Biology
Nigel Scott, Nick P. West, Alison Cairns, Olorunda Rotimi
Summary: Medullary carcinoma of the colon is often mistaken for poorly differentiated adenocarcinoma NOS and occasionally for neuroendocrine or metastatic carcinoma. Greater familiarity with morphological criteria and use of mismatch repair protein staining should improve diagnosis. Most medullary carcinomas exhibit mismatch repair deficiency, are located in ascending colon and caecum and have a lower rate of vascular channel invasion and lymph node metastasis compared to poorly differentiated adenocarcinoma.
Review
Oncology
Robin Park, Laercio Lopes, Sunggon Lee, Ivy Riano, Anwaar Saeed
Summary: BRAF mutations are associated with worse overall survival but do not affect the response to immune checkpoint inhibitors in patients with deficient mismatch repair/microsatellite instability-high colorectal cancer.
Article
Medicine, Research & Experimental
Jing Wang, Ruiyu Li, Junjie Li, Yuting Yi, Xiaoding Liu, Jingci Chen, Hui Zhang, Junliang Lu, Cami Li, Huanwen Wu, Zhiyong Liang
Summary: This study utilized DNA and RNA sequencing to detect fusion genes in dMMR CRCs, revealing that fusion-positive tumors tend to occur in older patients, are predominantly right-sided, and display mutations in key WNT pathway components.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Virology
XuSha Zhou, Jing Zhao, Jian V. Zhang, Yinglin Wu, Lei Wang, Xiaoqing Chen, Dongmei Ji, Grace Guoying Zhou
Summary: This study demonstrated that MEK inhibitor Trametinib can enhance oncolytic virus activity in BRAF V600E-mutated tumor cells, and combination therapy with oHSV and MEKi Trametinib can improve virus replication and show synergistic therapeutic efficacy in vivo for BRAF or KRAS-mutated tumors, providing new insights for clinical development.
Article
Oncology
Min Li, Congcong Zhu, Ying Xue, Changhong Miao, Ruiping He, Wei Li, Baolong Zhang, Wenqiang Yu, Xingxu Huang, Minzhi Lv, Ye Xu, Qihong Huang
Summary: This study aims to develop a reliable DNA methylation classifier for predicting recurrence risk and chemotherapy benefit in stage II colorectal cancer patients. By conducting genome-wide DNA methylation capture sequencing, a relapse-related DNA methylation signature consisting of eight CpG sites was identified. The classifier showed higher prognostic accuracy than clinicopathological risk factors.
BRITISH JOURNAL OF CANCER
(2023)
Article
Education, Scientific Disciplines
Michael Schoen, Konrad Steinestel, Doreen Spiegelburg, Annika Risch, Mira Seidel, Leon Schurr, Ulrich Kai Fassnacht, Nikola Golenhofen, Tobias Maria Boeckers, Anja Bockers
Summary: This study explores how to integrate scientific competencies into anatomical teaching by having students create scientific posters to cultivate their scientific skills. Results show that students invest extra time in collaboration, integrate primary literature in most posters, but also reveal some issues. Students show positive attitude towards learning new scientific skills, but some perceive the project as an additional burden in a demanding gross anatomy course.
ANATOMICAL SCIENCES EDUCATION
(2022)
Article
Pathology
Daniel Gagiannis, Vincent Gottfried Umathum, Wilhelm Bloch, Conn Rother, Marcel Stahl, Hanno Maximilian Witte, Sonja Djudjaj, Peter Boor, Konrad Steinestel
Summary: This study analyzed biopsy and autopsy samples from COVID-19 patients, revealing vascular, organizing, and fibrotic patterns of lung injury caused by COVID-19. The results suggest a rapid spread of SARS-CoV-2 from the upper airways to the lung periphery, with a diminishing viral load during the course of the disease.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2022)
Review
Oncology
Hanno M. Witte, Niklas Gebauer, Konrad Steinestel
Summary: Salivary gland carcinomas are rare and difficult to treat. New therapeutic approaches, such as molecularly targeted therapy and immune checkpoint inhibition, are needed. Comprehensive analysis of the genomic landscape and tumor microenvironment is crucial for optimizing and personalizing treatment.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jan C. Kamp, Lavinia Neubert, Maximilian Ackermann, Helge Stark, Christopher Werlein, Jan Fuge, Axel Haverich, Alexandar Tzankov, Konrad Steinestel, Johannes Friemann, Peter Boor, Klaus Junker, Marius M. Hoeper, Tobias Welte, Florian Laenger, Mark P. Kuehnel, Danny D. Jonigk
Summary: There is an increased prevalence of pulmonary fibrosis in COVID-19 survivors, but the underlying molecular mechanisms are not well understood. This multicentric study found that there is a shift from pro-inflammatory to fibrogenic activity after the first week of hospitalization in severe COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Yasutoshi Ito, Tetsuo Yamamoto, Kosuke Miyai, Junya Take, Harry Scherthan, Anna Rommel, Stefan Eder, Konrad Steinestel, Alexis Rump, Matthias Port, Nariyoshi Shinomiya, Manabu Kinoshita
Summary: The study showed that AA2G treatment could reduce radiation-induced intestinal damage without compromising the antitumor effectiveness of radiotherapy against bladder tumors in rats. AA2G treatment may promote a restoration of the M2 response as well as tissue remodeling and wound healing. Both AA2G-treated and control groups exhibited similar residual DNA damage in bladders and ilea seven days post-irradiation, suggesting effective tumor control in both groups.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2022)
Article
Oncology
Magdalene Cyra, Miriam Schulte, Ruth Berthold, Lorena Heinst, Esther-Pia Jansen, Inga Gruenewald, Sandra Elges, Olle Larsson, Christoph Schliemann, Konrad Steinestel, Susanne Hafner, Thomas Simmet, Eva Wardelmann, Sareetha Kailayangiri, Claudia Rossig, Ilka Isfort, Marcel Trautmann, Wolfgang Hartmann
Summary: In this study, we found that CREB is phosphorylated and activated in SySa, accompanied by the expression of downstream targets. Human mesenchymal stem cells engineered to express SS18-SSX promote CREB expression and phosphorylation. Conversely, RNAi-mediated knockdown of SS18-SSX impairs CREB phosphorylation in SySa cells. Inhibition of CREB activity reduces downstream target expression, accompanied by suppression of SySa cell proliferation and induction of apoptosis in vitro and in vivo.
Article
Oncology
Arthur Bauer, Niklas Gebauer, Juliana Knief, Lars Tharun, Nele Arnold, Armin Riecke, Konrad Steinestel, Hanno M. Witte
Summary: This study assessed the expression of CD39 and CD73 in different types of salivary gland carcinomas (SGCs) and found specific adenosine signaling patterns in different entities. Targeting the adenosine pathway may be a promising therapeutic option for selected entities.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Gastroenterology & Hepatology
Luisa Schonhart, Rene Kaiser, Roland Schmidt, Adrian Sailer, Konrad Steinestel, Wolfgang Schneider-Kappus, Christian Beltzer
Summary: This article presents a case of a rare primary squamous cell carcinoma (PSCC) of the stomach, including preoperative investigations, surgical treatment, histologic criteria and findings, and follow-up.
ZEITSCHRIFT FUR GASTROENTEROLOGIE
(2023)
Article
Oncology
Daniel Gagiannis, Anna Scheil, Sarah Gagiannis, Carsten Hackenbroch, Ruediger Horstkorte, Konrad Steinestel
Summary: In this study, the expression of polySia-NCAM in lung neuroendocrine neoplasms was investigated to determine its correlation with disease progression, treatment response, and prognosis. The majority of cases showed a strong staining signal for polySia-NCAM, but its expression did not significantly impact response to therapy or overall survival. However, it may still be a potential therapeutic target for individual tumor therapy.
Article
Oncology
Niklas Gebauer, Maria Ziehm, Judith Gebauer, Armin Riecke, Sebastian Meyhoefer, Birte Kulemann, Nikolas von Bubnoff, Konrad Steinestel, Arthur Bauer, Hanno M. Witte
Summary: This study compares several established risk scores and ratios in neuroendocrine neoplasms of the gastro-entero-pancreatic (GEP-NEN) system to evaluate their prognostic capabilities. The Glasgow Prognostic Score (GPS) was found to be the only independent predictor of survival across all stages of GEP-NEN, supporting its clinical utility for risk stratification in this group of patients.
Article
Pathology
Nathalie Maag, Annette Arndt, Konrad Steinestel
Summary: This study used laser microdissection (LMD) to identify intratumoral heterogeneity in colorectal cancer (CRC) and compared the results to routine methods. The results showed that LMD improved spatial resolution in the molecular analysis of small tumor areas and identified low-level mutations in different genes within the same patient sample.
Article
Pathology
Saskia von Stillfried, Benita Freeborn, Svenja Windeck, Peter Boor
Summary: The German Registry of COVID-19 Autopsies serves as the electronic backbone of the National Autopsy Network in Germany, collecting and studying a large amount of COVID-19 autopsy data and samples during the pandemic to promote medical development and understanding of the disease.
Article
Health Care Sciences & Services
Saskia von Stillfried, Roman David Buelow, Rainer Roehrig, Peter Boor
Summary: The German COVID-19 Autopsy Registry (DeRegCOVID) serves as a central hub for multicenter autopsy-based studies and provides support for researchers and data analysis. The registry shows that COVID-19 is the underlying cause of death in most autopsy cases, with diffuse alveolar damage and multi-organ failure being the most common immediate causes of death. The registry is of great importance for medical research, policy-making, and public discussion.
LANCET REGIONAL HEALTH-EUROPE
(2022)
Article
Critical Care Medicine
Saskia von Stillfried, Roman David Buelow, Rainer Roehrig, Patrick Meybohm, Peter Boor
Summary: This study analyzed data from the German COVID-19 autopsy registry and found that bleeding events were more frequent in ECMO cases, with a higher proportion of intracranial bleeding. The study suggests the potential value of autopsies and a joint interdisciplinary multicenter approach in addressing fatal complications in COVID-19.
Article
Oncology
Martin Faehling, Hanno Witte, Martin Sebastian, Matthias Ulmer, Rainer Saetzler, Konrad Steinestel, Wolfgang M. Brueckl, Georg Evers, Christian Meyer zum Bueschenfelde, Annalen Bleckmann
Summary: This retrospective analysis of locally resectable NSCLC patients who received neoadjuvant immuno(chemo)therapy and resection found that over two-thirds of patients had a major pathological response. Survival rates showed promising results.
THERAPEUTIC ADVANCES IN MEDICAL ONCOLOGY
(2022)