Article
Biochemistry & Molecular Biology
Maria Ungericht, Valeria Groaz, Moritz Messner, Thomas Schuetz, Luca Brunelli, Marc-Michael Zaruba, Daniela Lener, Eva Stocker, Axel Bauer, Alexander Stephan Kroiss, Agnes Mayr, Christoph Roecken, Gerhard Poelzl
Summary: This study evaluated the correlation between 99mTc-DPD scintigraphy and histological amyloid load. The results showed a significant correlation between cardiac 99mTc-DPD uptake and histological amyloid load. This study provides evidence for the use of cardiac 99mTc-DPD uptake measurements for risk stratification and guidance of therapy.
AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
(2023)
Article
Cardiac & Cardiovascular Systems
Ulrika Thelander, Gunilla T. Westermark, Gunnar Antoni, Sergio Estrada, Alice Zancanaro, Elisabet Ihse, Per Westermark
Summary: This study found that the binding of skeletal probes to amyloid-containing hearts is due to the presence of tiny calcification clusters, which are not directly associated with amyloid fibrils. Therefore, bone scans can be considered surrogate markers of ATTR amyloid, but caution is needed when estimating amyloid load or disease progression.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Angela Pucci, Alberto Aimo, Veronica Musetti, Andrea Barison, Giuseppe Vergaro, Dario Genovesi, Assuero Giorgetti, Silvia Masotti, Chiara Arzilli, Concetta Prontera, Luigi Emilio Pastormerlo, Michele Alessandro Coceani, Marco Ciardetti, Nicola Martini, Cataldo Palmieri, Claudio Passino, Claudio Rapezzi, Michele Emdin
Summary: The study found that amyloid and fibrosis contribute similarly to extracellular volume expansion in cardiac amyloidosis (CA), and their combination can better explain the correlation with cardiac magnetic resonance imaging and peripheral biomarkers compared to amyloid deposits alone.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Cardiac & Cardiovascular Systems
Franziska Seidel, Bernd Opgen-Rhein, Axel Rentzsch, Martin Boehne, Bardo Wannenmacher, Dorothee Boecker, Katja Reineker, Maria Grafmann, Gesa Wiegand, Tobias Hecht, Daniela Kiski, Marcus Fischer, Konstantin Papakostas, Bettina Ruf, Jennifer Kramp, Marcus Khalil, Michael Kaestner, Michael Steinmetz, Gunther Fischer, Sevinc Oezcan, Noa Freudenthal, Ulrich Schweigmann, Regina Hellwig, Thomas Pickardt, Karin Klingel, Daniel Messroghli, Stephan Schubert
Summary: Endomyocardial biopsy (EMB) provides important information for clinical decision-making in pediatric myocarditis patients. Children with fulminant clinical presentation, high amounts of mononuclear cell infiltrates, healing/chronic inflammation, and young age have the highest risk for major adverse cardiac events (MACE).
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Philippe Germain, Armine Vardazaryan, Aissam Labani, Nicolas Padoy, Catherine Roy, Soraya El Ghannudi
Summary: The aim of this study was to compare the classification of cardiac MR-images of AL versus ATTR amyloidosis by neural networks and human readers. The results showed that Cine-CNN had significantly better discrimination between AL and ATTR amyloidosis compared to Gado-CNN or human readers. However, the performance of Cine-CNN was still suboptimal for clinical practice.
Article
Cardiac & Cardiovascular Systems
Pranav Chandrashekar, Anish K. Desai, Barry H. Trachtenberg
Summary: The therapeutic landscape for cardiac amyloidosis is evolving rapidly, focusing on early identification and targeted therapy to prevent further deposition. Targeted therapeutics for transthyretin and light chain amyloidosis are being reviewed, with an emphasis on understanding the underlying mechanistic pathways of the diseases. Early recognition of cardiac adverse effects of therapies in patients with advanced amyloidosis can help prevent decompensation and guide therapy changes when necessary.
HEART FAILURE REVIEWS
(2022)
Article
Health Care Sciences & Services
Estefania Tarazon, Lorena Perez-Carrillo, Manuel Portoles, Esther Rosello-Lleti
Summary: This study focuses on analyzing new ultrastructural findings in cardiac biopsy rejection through the observation of perinuclear clustering of mitochondria, which could complement and improve the diagnosis of rejection.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Medicine, General & Internal
Anna Baritussio, Chun-yan Cheng, Giulia Lorenzoni, Cristina Basso, Stefania Rizzo, Monica De Gaspari, Francesco Fachin, Andrea Silvio Giordani, Honoria Ocagli, Elena Pontara, Maria Grazia Peloso Cattini, Elisa Bison, Nicoletta Gallo, Mario Plebani, Giuseppe Tarantini, Sabino Iliceto, Dario Gregori, Renzo Marcolongo, Alida Linda Patrizia Caforio
Summary: The role of inflammation markers in myocarditis is unclear. This study found that elevated CRP at diagnosis can identify patients with clinically suspected myocarditis and less severe clinical features, but does not contribute to predicting survival. The strongest predictors of death/heart transplant are reduced LVEF, biopsy-proven diagnosis, and positive ANA.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Nicolas L. Palaskas, Ana Segura, Laura Lelenwa, Bilal A. Siddiqui, Sumit K. Subudhi, Juan Lopez-Mattei, Jean B. Durand, Anita Deswal, Bihong Zhao, L. Maximilian Buja, Cezar Iliescu
Summary: A grading system was developed for ICI myocarditis based on degree of inflammatory infiltrate, with some patients able to continue ICI without immunosuppressive therapy and have good cardiovascular outcomes. Further studies are needed to identify low-risk patients who can safely receive ICI therapy.
EUROPEAN JOURNAL OF HEART FAILURE
(2021)
Article
Clinical Neurology
Diane Beauvais, Celine Labeyrie, Cecile Cauquil, Bruno Francou, Ludivine Eliahou, Adeline Not, Andoni Echaniz-Laguna, Clovis Adam, Michel S. Slama, Anouar Benmalek, Luca Leonardi, Francois Rouzet, David Adams, Vincent Algalarrondo, Guillemette Beaudonnet
Summary: Multimodal neurological and cardiac evaluation is crucial for the early detection of hereditary transthyretin amyloidosis and initiation of disease-modifying ATTRv treatments (DMTs).
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2023)
Review
Medicine, General & Internal
Danielle M. Haanschoten, Ahmet Adiyaman, Nils A. 't Hart, Piet L. Jager, Arif Elvan
Summary: This study describes the use of electro-anatomic mapping (EAM)-guided endomyocardial biopsy (EMB) in patients with cardiac sarcoidosis (CS), showing that unipolar voltage mapping may be superior in targeting active inflamed tissue.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Cardiac & Cardiovascular Systems
Daniel Kiblboeck, Karin Klingel, Martin Genger, Selina Traxler, Nathalie Braunsteiner, Clemens Steinwender, Joerg Kellermair
Summary: The European Medicine Agency reported a case series of suspected acute myocarditis following mRNA COVID-19 vaccination. Endomyocardial biopsies were found to be crucial in establishing a correct diagnosis of myocarditis. Despite rare severe adverse events, the value of COVID-19 immunization for the global community should not be undermined.
Article
Cardiac & Cardiovascular Systems
Renzo Marcolongo, Stefania Rizzo, Alessia Cerutti, Elena Reffo, Biagio Castaldi, Anna Baritussio, Cristina Basso, Giovanni Di Salvo, Alida L. P. Caforio
Summary: This study reported three cases of pediatric autoimmune/immune-mediated myocarditis successfully treated with immunosuppressive therapy (IT). IT was associated with a substantial and sustained recovery of cardiac function in the patients, regardless of their heterogeneous clinical and pathological features. Combination IT was well tolerated and enabled tapering and weaning off steroids.
Article
Cardiac & Cardiovascular Systems
Mizuki Ida, Shiro Nakamori, Shinya Yamamoto, Seimi Watanabe, Kyoko Imanaka-Yoshida, Masaki Ishida, Hajime Sakuma, Keiichi Yamanaka, Kaoru Dohi
Summary: In this article, we present a complex case of an 81-year-old female patient who experienced persistently high levels of troponin after immunotherapy and displayed amyloid deposition in the myocardium. However, histological examination revealed minimal T-lymphocyte infiltration and no myocyte necrosis, indicating transthyretin cardiac amyloidosis rather than immune checkpoint inhibitor myocarditis. This case highlights the importance of evaluating other causes of elevated troponin levels and the necessity of comprehensive histological and immunohistochemical examinations when cardiovascular magnetic resonance imaging is inconclusive.
Article
Pathology
Edyta Reichman-Warmusz, Krzysztof Kaczmarek, Arkadiusz Badzinski, Oliwia Warmusz, Romuald Wojnicz
Summary: Our study suggests that de-novo expression of E-selectin in endothelial cells may be a useful marker of chronic inflammation in patients with heart failure secondary to dilated cardiomyopathy.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Article
Clinical Neurology
Hajrullah Ahmeti, Eva Juettner, Christoph Roecken, Olav Jansen, Matthias Laudes, Michael Synowitz
Summary: Pituitary gland metastases are rare and often diagnosed during autopsies. The most common primary tumors that metastasize to the pituitary gland are breast, lung, thyroid, and renal carcinomas. We present a rare case of spindle cell rhabdomyosarcoma metastasizing to the pituitary gland. The patient experienced symptoms such as headache, visual disorder, panhypopituitarism, and diabetes insipidus. Despite the inability to surgically remove the tumor, biopsy confirmed the diagnosis and the patient received chemotherapy and irradiation. Even high-risk patients can achieve remission after treatment.
JOURNAL OF NEUROLOGICAL SURGERY PART A-CENTRAL EUROPEAN NEUROSURGERY
(2023)
Review
Cardiac & Cardiovascular Systems
Maria Ungericht, Julia Wanschitz, Alexander S. Kroiss, Christoph Roecken, Thomas Schuetz, Moritz Messner, Marc-Michael Zaruba, Wolfgang N. Loescher, Gerhard Poelzl
Summary: This article describes two patients with ATTR amyloid myopathy, one with ATTR variant amyloidosis (ATTRv) and one with wild-type ATTR amyloidosis (ATTRwt). The ATTRwt patient showed muscular tracer uptake consistent with ATTR amyloid myopathy, while in the ATTRv patient, myopathy occurred after neuropathy and cardiomyopathy. Muscle biopsies confirmed the presence of interstitial amyloid deposits, particularly high in the adipose tissue. This case report highlights the frequent concomitant presence of cardiac ATTR amyloidosis and ATTR amyloid myopathy.
JOURNAL OF NUCLEAR CARDIOLOGY
(2023)
Article
Endocrinology & Metabolism
Darab Ghadimi, Michael Ebsen, Christoph Roecken, Regina Foelster-Holst, Birte Groessner-Schreiber, Christof Doerfer, Wilhelm Bockelmann
Summary: Dietary habits and food can lead to oral dysbiosis, which affects overall health and can lead to systemic diseases. Fermentable carbohydrates in modern diets, such as processed starch and synthetic carbohydrates, are risk factors for oral dysbiosis. The interconnectivity of oral, lung, and gut microbiomes is not well understood, particularly regarding the role of lactobacilli and Lactobacillus phages in rescuing dysbiotic effects.
ENDOCRINE METABOLIC & IMMUNE DISORDERS-DRUG TARGETS
(2023)
Article
Biochemistry & Molecular Biology
Julian Baur, Natalie Berghaus, Sarah Schreiner, Ute Hegenbart, Stefan O. Schoenland, Sebastian Wiese, Stefanie Huhn, Christian Haupt
Summary: This study analyzed the exact primary structure of AL proteins and their precursor LCs in 10 lambda AL amyloidosis patients. Results showed that all AL proteins had a common C-terminal truncation region and a significant increase in the isoelectric point of V-L. Moreover, no evidence of other common post-translational modifications was found.
AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
(2023)
Article
Oncology
Magnus Kock Am Brink, Laura Sophie Dunst, Hans-Michael Behrens, Sandra Krueger, Thomas Becker, Christoph Roecken
Summary: The study investigates the problem of intratumoral heterogeneity (ITH) in gastric cancer (GC), and tests the effects of Ki67 and tumor regression on ITH after neoadjuvant/perioperative chemotherapy. The results suggest that Ki67 is significantly associated with tumor regression and may serve as a predictor for chemotherapy sensitivity and an indicator of ITH.
BRITISH JOURNAL OF CANCER
(2023)
Article
Clinical Neurology
Ulf Jensen-Kondering, Nils G. Margraf, Caroline Weiler, Walter Maetzler, Justina Dargvainiene, Kim Falk, Sarah Philippen, Thorsten Bartsch, Charlotte Flueh, Christoph Roecken, Bettina Moeller, Georg Royl, Alexander Neumann, Norbert Brueggemann, Benjamin Roeben, Claudia Schulte, Benjamin Bender, Daniela Berg, Gregor Kuhlenbaeumer
Summary: This study compared the clinical, radiological, and cerebrospinal fluid marker data of patients with CAA, MLH, AD, and healthy controls. The results showed that cSS was more common in CAA, and MLH with cSS was associated with ICH. The concentrations of cerebrospinal fluid markers differed among the groups.
INTERNATIONAL JOURNAL OF STROKE
(2023)
Article
Biochemistry & Molecular Biology
Tim A. Steiert, Genis Parra, Marta Gut, Norbert Arnold, Jean-Remi Trotta, Raul Tonda, Alice Moussy, Zuzana Gerber, Peter M. Abuja, Kurt Zatloukal, Christoph Roecken, Trine Folseraas, Marit M. Grimsrud, Arndt Vogel, Benjamin Goeppert, Stephanie Roessler, Sebastian Hinz, Clemens Schafmayer, Philip Rosenstiel, Jean-Francois Deleuze, Ivo G. Gut, Andre Franke, Michael Forster
Summary: Formalin fixation with paraffin-embedding (FFPE) was developed in the late 19th century and is still commonly used in clinical practice. However, FFPE samples can undergo DNA damage, leading to potential errors in sequencing and analysis. This review discusses mitigation strategies in sample quality control, DNA repair treatments, sample preparation, and bioinformatic analysis of FFPE-DNA.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Bente Holm, Stephan Barsuhn, Hans-Michael Behrens, Sandra Krueger, Christoph Roecken
Summary: This study explored the expression and correlation of RNF43 and LRP1B with clinicopathological characteristics in gastric cancer. The expression of RNF43 and LRP1B was significantly correlated with the Lauren phenotype and with each other. Furthermore, differential effects of RNF43 expression were observed between intestinal and diffuse type gastric cancers. These findings suggest that the expression and biological effects of RNF43 and LRP1B in gastric cancer are context-dependent.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Katharina Pries, Sandra Krueger, Steffen Heckl, Hans-Michael Behrens, Christoph Roecken
Summary: Recent studies have found a connection between certain subunits of the SWI/SNF complex and specific tumor characteristics in gastric cancer. This study aimed to examine the expression patterns of SMARCA4 and SMARCE1, two subunits of the SWI/SNF complex, in gastric cancer and their potential relationship with ERBB2. The results showed significant correlations between the expressions of SMARCA4 and SMARCE1 with ARID1A, p53, and microsatellite status, but not with patient prognosis. Amplification analysis revealed co-amplification of SMARCE1 and ERBB2 in some cases, suggesting potential synergistic effects for improved treatment of gastric cancer.
Article
Clinical Neurology
Monika Huhndorf, Christoph Roecken, Charlotte Flueh, Caroline Weiler, Gregor Kuhlenbaeumer, Nora Tegeler, Hannes Schacht, Alexander Neumann, Nils G. Margraf, Ulf Jensen-Kondering
Summary: This study aimed to estimate the frequency of deep-seated microbleeds on MRI in patients with lobar hemorrhages and histopathological evidence for cerebral amyloid angiopathy (CAA). The findings showed that approximately 15% of patients with histopathologically diagnosed CAA have deep-seated microbleeds.
FRONTIERS IN NEUROLOGY
(2023)
Review
Biochemistry & Molecular Biology
Francesco Marchi, Chiara Kessler, Daniela Distefano, Lodovico Terzi di Bergamo, Luca Fumagalli, Manuela Averaimo, Emanuele Crupi, Fabio Bergamini, Giorgia Melli, Georg Stussi, Davide Rossi, Claudio Gobbi, Paolo Ripellino, Emanuele Pravata, Dominique E. Kuhlen, Christoph Roecken, Pietro Scarone, Bernhard Gerber, Adalgisa Condoluci
Summary: This study found that amyloid, mostly of the ATTR subtype, was detected in over three quarters of patients undergoing surgery for lumbar spinal stenosis, and it is associated with age and ligamentum flavum thickness. These findings suggest that histopathological examination of the ligamentum flavum may be helpful for future decision making.
AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
(2023)
Article
Pathology
Samir Abdullazade, Hans-Michael Behrens, Sandra Krueger, Jochen Haag, Christoph Roecken
Summary: The study validated gene amplification, protein expression, and tumor biological function of MDM2 in gastric cancer, and found a significant correlation between MDM2 amplification and protein expression. However, MDM2 amplification and expression were not associated with patient survival.
Article
Geriatrics & Gerontology
Patricia Huebbe, Stephanie Bilke, Johanna Rueter, Anke Schloesser, Graeme Campbel, Claus-C. Glueer, Ralph Lucius, Christoph Roecken, Andreas Tholey, Gerald Rimbach
Summary: Recent studies have found that the human APOE epsilon 4 allele protects against non-alcoholic fatty liver disease (NAFLD), while epsilon 3 promotes hepatic steatosis and steatohepatitis. In this study, mice expressing the APOE3 or APOE4 protein isoforms were fed a high-fat and high-sucrose diet to induce obesity. The results showed that APOE3 mice had higher liver weights and exhibited more severe hepatic steatosis compared to APOE4 mice. Proteome analysis also revealed differences in proteins related to inflammation, damage response, and lipid storage between APOE3 and APOE4 mice. These findings support the association of increased NAFLD risk with the APOE epsilon 3 allele and suggest potential mechanisms of protection conferred by APOE epsilon 4.
Article
Medicine, Research & Experimental
Hajrullah Ahmeti, Amke Caliebe, Christoph Rocken, Olav Jansen, Maximilian H. Mehdorn, Michael Synowitz
Summary: This study systematically examined 696 patients with primary intracranial meningiomas to assess the effect of preoperative peritumoral brain edema (PTBE) on preoperative symptoms, neurological deficits, postoperative complications, and long-term outcomes. Results showed that PTBE significantly increased the incidences of specific preoperative symptoms, neurological deficits, and postoperative complications. Patients with preoperative PTBE required more medical support after surgery, but all patients had favorable outcomes.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Hematology
Lukas John, Sandra Sauer, Ute Hegenbart, Peter Dreger, Michael Hundemer, Carsten Mueller-Tidow, Anita Schmitt, Michael Schmitt, Marc S. Raab, Stefan O. Schoenland
Summary: This study assessed the safety and efficacy of donor-derived CAR-T therapy in myeloma patients with prior allo-SCT. The treatment was well tolerated, with no observed adverse effects such as neurotoxicity syndrome or cytokine release syndrome. Importantly, no new graft-versus-host disease was observed, and all patients responded well to treatment.
TRANSPLANTATION AND CELLULAR THERAPY
(2023)
