Journal
VIRCHOWS ARCHIV
Volume 454, Issue 4, Pages 401-409Publisher
SPRINGER
DOI: 10.1007/s00428-009-0739-5
Keywords
Proliferation; ICC-MY; ICC-DMP; TUNEL. BrdU
Categories
Funding
- National Science Foundation of China (NSFC) [2007CB512401, 30800982]
- Natural Science Foundation [2008BB5280]
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This study aimed at evaluating whether apoptosis of interstitial cells of Cajal (ICC), smooth muscle cells (SMC), and enteric neurons was involved in a guinea pig model of intestinal ischemia and reperfusion injury. The small intestinal segments were resected at either 6 (I-60/R-6h) and 12 h (I-60/R-12h) or 7 (I-60/R-7d) to 14 (I-60/R-14d) days after 60 min intestinal ischemia in the adult guinea pigs and studied by immunohistochemistry with anti-Kit, 5-bromo-2'-deoxyuridine (BrdU), alpha-smooth muscle actin, vimentin, and beta-tublin III antibodies. Also, apoptosis was tested by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method. In the I-60/R-12h injury, there was a similar to 50% decrease of Kit+ cells in cell numbers at the level of myenteric plexus and a number of Kit-/vimentin-positive cells were labeled by TUNEL. Also, a few SMC and enteric neurons were TUNEL positive. The Kit+ ICC recovered to normal and a number of Kit-/BrdU-double-positive cells were observed in the I-60/R-14d group. Our results indicated that the intestinal I/R injury could lead to apoptosis of ICC, SMC, and enteric neurons which may contribute to the gastrointestinal motility disorders, and proliferation was involved in the recovery of ICC.
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