Article
Chemistry, Analytical
Alice Spadea, Joanna Denbigh, M. Jayne Lawrence, Mustafa Kansiz, Peter Gardner
Summary: This study demonstrates the first use of a novel completely optically-based photothermal method to obtain infrared spectra of biological cells and to combine it with Raman spectra at submicron spatial resolution. The new instrumentation is capable of analyzing subcellular structures, identifying protein secondary structures, and lipid-rich bodies on the submicron scale, showing great potential in cell analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
David Hartnell, Ashley Hollings, Anna Maria Ranieri, Hum Bahadur Lamichhane, Thomas Becker, Nicole J. Sylvain, Huishu Hou, M. Jake Pushie, Elizabeth Watkin, Keith R. Bambery, Mark J. Tobin, Michael E. Kelly, Massimiliano Massi, Jitraporn Vongsvivut, Mark J. Hackett
Summary: This study presents advancements in sub-cellular biochemistry research using SR-ATR-FTIR microspectroscopy, showcasing the capability to directly image sub-cellular protein aggregates and lipid inclusions. The choice of spectral resolutions and the use of a focal plane array detector are highlighted for their impact on data collection speed and image quality. The information provided in the study emphasizes the potential of spectroscopic methods for interdisciplinary research and expanding areas of study in the biological sciences.
Article
Biochemistry & Molecular Biology
Natasha Josifovska, Sofija Andjelic, Lyubomyr Lytvynchuk, Xhevat Lumi, Tanja Ducic, Goran Petrovski
Summary: Synchrotron radiation-based Fourier Transform Infrared (SR-FTIR) microspectroscopy is a technique used for non-destructive and chemically sensitive detection of biomolecular changes in cells. This study investigated the effects of autophagy and oxidative stress on human primary retinal pigment epithelium (hRPEs), using SR-FTIR microspectroscopy to analyze the changes in nucleic acids, proteins, carbonyl groups, and lipids. The results can be used to study drug discovery targeting autophagy and oxidative stress in age-related macular degeneration (AMD).
Article
Infectious Diseases
Amal G. G. Al-Bakri, Lina A. A. Dahabiyeh, Enam Khalil, Deema Jaber, Gihan Kamel, Nina Schleimer, Christian Kohler, Karsten Becker
Summary: In this study, synchrotron-radiation-based Fourier transform infrared (SR-FTIR) was used to investigate the differences between small colony variants (SCVs) of Staphylococcus aureus and diabetic foot Staphylococcus epidermidis. The results showed that SR-FTIR can effectively discriminate between different staphylococci species and differentiate SCVs from their corresponding natural strains.
Article
Biochemistry & Molecular Biology
Hossam Taha Mohamed, Gihan Kamel, Noura El-Husseiny, Aya Ali El-Sharkawy, Ahmed A. El-Sherif, Mohamed El-Shinawi, Mona Mostafa Mohamed
Summary: This study suggests that the pathogenesis of inflammatory breast cancer is related to inflammatory manifestations accompanied by specific cellular and molecular mechanisms in the tumor microenvironment. Tumor-associated macrophages play a significant role in the metastatic process of IBC through the secretion of cytokines. Gene expression signature and SR-mu FTIR were used to study the changes in macrophages stimulated by the secretome of IBC and non-IBC cells, showing significant differences in polarization-related genes and biochemical changes between the two. This research helps to better understand the potential role of TAMs in IBC.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Pavel Seredin, Dmitry Goloshchapov, Vladimir Kashkarov, Anatoly Lukin, Yaroslav Peshkov, Ivan Ippolitov, Yuri Ippolitov, Tatiana Litvinova, Jitraporn Vongsvivut, Boknam Chae, Raul O. Freitas
Summary: This study utilizes infrared spectral analysis of protein secondary structure in dental biofilms to predict cariogenic status of the oral cavity. The study also found that the application of a medicinal agent, known as a modulator, showed strong sensitivity to the spectral markers. This research provides the foundation for infrared spectral diagnosis of changes in the oral microbiome due to tooth decay and for the selection of optimal therapeutic treatments based on microbiome-directed prevention measures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Takahiro Iwai, Sadao Honda, Shimpei Watanabe, Ritsuko Matsushita, Toshio Nakanishi, Masahisa Takatsu, Taro Moriwaki, Makina Yabashi, Tetsuya Ishikawa, Yasuo Seto
Summary: Quantitative evaluation of drug mixing condition for forensic discrimination of drug powders was conducted using micro Fourier transform infrared (FT-IR) spectroscopy. Different mixing modes were tested by mixing, homogenizing, dissolving, and drying processes. The spatial localization and distribution of the drugs were analyzed and evaluated using synchrotron radiation (SR) or globar light with different instruments. The differentiation among the mixing modes was assessed using mixing parameters including the relative content ratio (RCR) and extreme value frequency (%EV) as mixing indices. The discrimination capacity of the SR instrument was found to be superior to that of the globar light instrument.
Review
Instruments & Instrumentation
Ahmed Refaat, Gihan Kamel
Summary: This article introduces the important role of synchrotron radiation-FTIR microspectroscopy in the field of biomedical research, with a focus on neurodegenerative diseases, diabetes, preeclampsia, and other relevant areas.
APPLIED SPECTROSCOPY REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Clinton J. Kidman, Cyril D. S. Mamotte, M. Adrien Eynaud, Juliane Reinhardt, Jitraporn Vongsvivut, Mark J. Tobin, Mark J. Hackett, Ross M. Graham
Summary: Hepatocytes play a crucial role in maintaining the homeostasis of iron and lipid metabolism in mammals, with dysregulation potentially leading to serious health consequences such as non-alcoholic fatty liver disease (NAFLD). Research has shown that iron loading in cells can disrupt lipid metabolism, contributing to the development of hepatic steatosis.
BIOCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Raquel C. C. Laginha, Clara B. B. Martins, Ana L. C. Brandao, Joana Marques, M. Paula M. Marques, Luis A. E. Batista de Carvalho, Ines P. Santos, Ana L. M. Batista de Carvalho
Summary: In this study, the cytotoxic effects of a complex with two Pd(II) centers (Pd(2)Spm) on healthy and cancerous prostate human cell lines were evaluated and compared to cisplatin. Fourier Transform Infrared (FTIR) and Raman microspectroscopies were used to understand the molecular mechanisms of action of both drugs, revealing differences in their impact on DNA, lipids, and proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Supatcharee Siriwong, Waraporn Tanthanuch, Duangjai Srisamut, Chulalak Chantarakhon, Kanokwan Kamkajon, Kanjana Thumanu
Summary: The development of biochemical analysis techniques to study heterogeneous biological samples has led to the comparison of the efficiencies of SR-FTIR microspectroscopy and FPA-FTIR microspectroscopy. The results show that SR-FTIR microspectroscopy can classify rice tissue components more efficiently and reveal the biochemical distribution in rice tissue more effectively than FPA-FTIR microspectroscopy.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Multidisciplinary Sciences
Maram Diab, Al-Sayed Sallam, Imad Hamdan, Randa Mansour, Rohanah Hussain, Giuliano Siligardi, Nidal Qinna, Enam Khalil
Summary: Insulin mucoadhesive buccal films (MBF) are a noninvasive delivery system formulated with chitosan, glycerin, and L-arginine. Stability testing showed retention of insulin structure and in vivo testing revealed a blood glucose-lowering effect in rat models, suggesting MBFs as a promising insulin delivery system.
Article
Chemistry, Multidisciplinary
Juan Reyes-Herrera, Damaris Acosta-Slane, Hiram Castillo-Michel, Ana Pradas E. del Real, Katarina Vogel-Mikus, Federico Benetti, Marco Roman, Julie Villanova, M. Cecilia Valles-Aragon
Summary: TiO2 nanoparticles are widely used in various industries and can accumulate in wastewater sludges. A study on sludge samples from wastewater treatment plants in Chihuahua State, Mexico revealed the presence of TiO2 nanoparticles of different forms, which may pose potential toxicity to agro-systems.
Article
Instruments & Instrumentation
Jie Shi, Ling Zong, Peiyao Wang, Chao Li, Jianjun Yue, Xin Wang
Summary: FTIR microspectroscopy was used to investigate apoptosis of pancreatic cancer PNAC-1 cells induced by DRE. The study showed significant changes in proteins and biomacromolecular structure and content, suggesting that FTIR microspectroscopy could be a potential tool for apoptotic study.
INFRARED PHYSICS & TECHNOLOGY
(2022)
Article
Instruments & Instrumentation
Gihan Kamel, Stephane Lefrancois, Thierry Moreno, Mohammad Al-Najdawi, Yazeed Momani, Anas Abbadi, Giorgio Paolucci, Paul Dumas
Summary: SESAME is the only synchrotron light facility in the Middle East and neighboring regions, providing infrared spectromicroscopy services for the scientific community in the Middle East. The facility has a collaboration agreement with the French synchrotron facility SOLEIL, and the currently operational infrared beamline has been designed and implemented to meet the demands of various scientific communities in the Middle East.
JOURNAL OF SYNCHROTRON RADIATION
(2021)
Review
Environmental Sciences
Zhe (Han) Weng, Johannes Lehmann, Lukas Van Zwieten, Stephen Joseph, Braulio S. Archanjo, Bruce Cowie, Lars Thomsen, Mark J. Tobin, Jitraporn Vongsvivut, Annaleise Klein, Casey L. Doolette, Helen Hou, Carsten W. Mueller, Enzo Lombi, Peter M. Kopittke
Summary: Soil organic carbon management is a nature-based method for carbon dioxide removal, which also enhances soil health and agricultural productivity. Recent theories emphasize the importance of molecular diversity, spatial heterogeneity, and temporal variability of organic compounds in controlling the formation and persistence of soil organic matter (SOM). Advanced in situ techniques are needed to study these processes without disturbing the physical and chemical integrity of the sample.
CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Applied
Mitra Nosratpour, Kamila Kochan, Jisheng Ma, Yong Wang, Bayden R. Wood, Victoria S. Haritos, Cordelia Selomulya
Summary: The structural polymorphism of SLPs can lead to expulsion of the protected component during storage. Changes in thermal behavior, crystalline properties, and fatty acid distribution occur in SLPs at different temperatures. The intact shape and stability of the core of SLPs are related to the outer layer of saturated fatty acids.
Article
Chemistry, Analytical
Thulya Chakkumpulakkal Puthan Veettil, Bayden R. Wood
Summary: Serum is a valuable source for proteomics analysis, but the high concentration range of protein/peptide species makes it challenging to detect low-molecular-weight compounds. In this study, a combination of near-infrared and mid-infrared spectroscopy was used to improve the detection limit of LMW amino acids in serum.
Article
Polymer Science
Andrea Costanzo, Alice Poggi, Stan Looijmans, Deepak Venkatraman, Dan Sawyer, Ljiljana Puskar, Claire Mcllroy, Dario Cavallo
Summary: Extensive research has been conducted in the field of material-extrusion (Mat-Ex) 3D printing to improve the inter-layer bonding process. However, little attention has been given to the fundamental molecular properties of the feedstock and their impact on the microstructure of the weld. This study demonstrates that weld strength can be increased by decreasing the average molar mass of the polymer, and proposes that molar mass should be considered as a key control parameter for achieving high weld strength in Mat-Ex.
Article
Polymer Science
Stan F. S. P. Looijmans, Michelle M. A. Spanjaards, Ljiljana Puskar, Dario Cavallo, Patrick D. Anderson, Lambert C. A. van Breemen
Summary: The development of fiber-reinforced polymer composites during processing is not solely determined by the flow field or the nucleating ability of the fiber surface, but rather by a synergistic combination of the two. The rheological characteristics of the material have a greater influence on the interphase structure formation than perturbations in experimental conditions. Thus, material selection is crucial in the design of hybrid and lightweight composite technologies.
Article
Chemistry, Analytical
Molong Han, Daniel Smith, Soon Hock Ng, Tomas Katkus, Aravind Simon John Francis Rajeswary, Periyasamy Angamuthu Praveen, Keith R. Bambery, Mark J. Tobin, Jitraporn Vongsvivut, Saulius Juodkazis, Vijayakumar Anand
Summary: In this study, phase imaging of biochemical samples was demonstrated for the first time at the Australian Synchrotron's Infrared Microspectroscopy (IRM) beamline using the usually disregarded near-IR (NIR) region of the synchrotron-IR beam. By aligning a pinhole with one of the lobes of the fork-shaped beam and applying a rapid phase-retrieval algorithm, promising preliminary results were obtained for the development of multimodal imaging capabilities at the IRM beamline.
Article
Chemistry, Analytical
Thulya Chakkumpulakkal Puthan Veettil, Rebekah N. Duffin, Supti Roy, Jitraporn Vongsvivut, Mark J. Tobin, Miguela Martin, John A. Adegoke, Philip C. Andrews, Bayden R. Wood
Summary: Neglected tropical diseases, including leishmaniasis, are becoming more widespread. This study demonstrates the potential of using synchrotron Fourier transform infrared microspectroscopy and machine learning models to accurately predict Leishmania infection. The use of this technique could pave the way for future clinical studies using portable devices.
ANALYTICAL CHEMISTRY
(2023)
Article
Instruments & Instrumentation
Miguela Martin, Molin Wongwattanakul, Noppmats Khemtonglang, Pakaphan Kiatchoosakun, Philip Heraud, Patcharee Jearanaikoon, Bayden R. Wood
Summary: Glucose-6 phosphate dehydrogenase (G6PD) deficiency is a common blood disease, affecting 400 million people globally, especially in malaria-endemic regions. Accurate diagnosis of this deficiency is crucial for malaria eradication efforts. This study investigates the use of attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) as a potential diagnostic technique. Results show that ATR FT-IR combined with multivariate data analysis can accurately classify fully deficient participants and partially deficient females who are often misdiagnosed. This approach has the potential to become a frontline screening tool, improving tailored drug treatments and saving lives.
APPLIED SPECTROSCOPY
(2023)
Review
Instruments & Instrumentation
Callum Gassner, Jitraporn Vongsvivut, Soon Hock Ng, Meguya Ryu, Mark J. J. Tobin, Saulius Juodkazis, Junko Morikawa, Bayden R. R. Wood
Summary: This review provides an overview of the knowledge gathered through polarized infrared spectroscopy on biological materials, including biomolecules, cells, and tissues. The review discusses the factors that can affect the analysis, such as sample preparation, sample properties, and instrumentation. It also highlights the potential impacts of using inherently polarized light sources for biological sample analysis.
APPLIED SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Alexander Veber, Victor M. R. Zancajo, Ljiljana Puskar, Ulrich Schade, Janina Kneipp
Summary: The mechanical and chemical properties of plant cell walls depend on the supramolecular assembly of cellulose fibrils. Diffraction limited infrared micro-spectroscopic mapping experiments were conducted to study the local orientation of cellulose in secondary plant cell walls. Results showed significant anisotropy of cellulose macromolecules and other biopolymers in different regions of the cross section. This study demonstrates the use of diffraction limited IR micro-spectroscopy in studying hierarchically structured materials with complex anisotropic behavior.
Article
Chemistry, Analytical
Thulya Chakkumpulakkal Puthan Veettil, Diana Alves, Jitraporn Vongsvivut, Rosemary L. Sparrow, Bayden R. Wood, Gil Garnier
Summary: Oxidative treatment of RBCs prior to freeze-drying stabilizes the cells for storage at room temperature. Synchrotron-based FTIR microspectroscopy was used to analyze single RBCs and compare lipid and protein spectral data before and after oxidation and freeze-drying. The analysis showed that oxidized and freeze-dried RBCs have different spectral profiles compared to untreated RBCs, indicating changes in lipid peroxidation, stiffness of the membrane, and protein secondary structure. Freeze-drying did not introduce additional changes. This study highlights the potential of freeze-dried RBCs as a stable source for blood serology testing and demonstrates the power of synchrotron FTIR microspectroscopic live-cell analysis.
Article
Chemistry, Analytical
John A. Adegoke, Callum Gassner, Varun J. Sharma, Sheila K. Patel, Louise Jackett, Isaac O. Afara, Jaishankar Raman, Louise M. Burrell, Bayden R. Wood
Summary: This study demonstrates that a portable handheld near-infrared spectrometer coupled with machine learning algorithms can effectively differentiate between kidney and cardiac fibrosis in a rat model of kidney failure, providing a potential intraoperative surgical tool for detecting and quantifying fibrosis in the heart and kidney.
ANALYSIS & SENSING
(2023)
Article
Chemistry, Analytical
Zack Richardson, Adele Kincses, Elif Ekinci, David Perez-Guaita, Karin Jandeleit-Dahm, Bayden R. Wood
Summary: In this study, a method combining ultracentrifugation, infrared spectroscopy, and machine learning was developed to accurately quantify low level microalbuminuria in urine samples. The results showed high sensitivity and specificity in discriminating between normal and micro/macroalbuminuric samples, and regression analysis provided quantification of albumin concentration. This approach is cost-effective, quick, and portable, making it a valuable tool for early screening of diabetic kidney disease.
ANALYSIS & SENSING
(2023)
Article
Chemistry, Analytical
John A. Adegoke, Hannah Raper, Callum Gassner, Philip Heraud, Bayden R. Wood
Summary: This study successfully discriminated different stages of functional asexual blood stage parasites using visible microspectrophotometry combined with machine learning, providing an important method for monitoring the progression and recovery of malaria.
Article
Biochemical Research Methods
Amir Pourabed, Thulya Chakkumpulakkal Puthan Veettil, Citsabehsan Devendran, Prasanna Nair, Bayden R. Wood, Tuncay Alan
Summary: Malaria, a life-threatening disease transmitted by infected mosquitoes, requires better diagnostic platforms to improve detection sensitivity and reduce processing times and costs. With the use of an acoustically actuated microfluidic mixer, blood cell lysis can be enhanced, leading to increased efficiency in releasing hemoglobin into plasma. The combination of acoustofluidic/spectroscopic approach shows promise for point-of-care blood diagnosis, especially in detecting neonatal and congenital malaria.
Article
Chemistry, Analytical
Elena Naumovska, Gabriel Kofi Nzulu, Laura Mazzei, Arnaud Le Febvrier, Kristina Komander, Martin Magnuson, Max Wolff, Per Eklund, Maths Karlsson
Summary: Results from an infrared spectroscopy study on hydrated nanocrystalline films of BaZr1-xScxO3-x/2 with different Sc concentrations show variations in the O-H stretching bands, indicating different proton configurations. Higher Sc concentrations exhibit a distinct O-H stretching band, while lower Sc concentrations show a broader, weaker band.
VIBRATIONAL SPECTROSCOPY
(2024)