Article
Parasitology
Tania Rojas-Campos, Froylan Ibarra-Velarde, Yolanda Vera-Montenegro, Miguel Flores-Ramos, Irene Cruz-Mendoza, Gerardo Leyva-Gomez, Alicia Hernandez-Campos
Summary: This study compared the fasciolicidal efficacy of a new injectable formulation of fosfatriclaben with subcutaneous closantel and oral triclabendazole. Results showed that fosfatriclaben and triclabendazole had similar efficacy, but fosfatriclaben's intramuscular administration was more convenient and had lower dosage.
VETERINARY PARASITOLOGY
(2021)
Article
Parasitology
Froylan Ibarra-Velarde, Tania Rojas-Campos, Yolanda Vera-Montenegro, Miguel Flores-Ramos, Rafael Castillo, Gerardo Leyva-Gomez
Summary: The study aimed to determine the effective dose of fosfatriclaben through a field study in sheep naturally infected with F. hepatica. Results showed that fosfatriclaben had a high fasciolicidal efficacy similar to triclabendazole, with only half the dose required to remove eggs and flukes in the sheep studied.
PARASITOLOGY RESEARCH
(2022)
Article
Veterinary Sciences
Rafael Zafra, Leandro Buffoni, Raul Perez-Caballero, Veronica Molina-Hernandez, Maria T. Ruiz-Campillo, Jose Perez, Alvaro Martinez-Moreno, Francisco J. Martinez Moreno
Summary: In this study, sheep were vaccinated with two different vaccines containing a cocktail of antigens and formulated with different adjuvants. The results showed that the first vaccine significantly reduced fluke burden, decreased egg output, and alleviated hepatic lesions. Additionally, animals in the first vaccine group exhibited higher levels of IgG1 and IgG2 in the serological study, supporting the hypothesis of protection found in this group.
VETERINARY RESEARCH
(2021)
Article
Microbiology
Martha V. Fernandez-Baca, Cristian Hoban, Rodrigo A. Ore, Pedro Ortiz, Young-Jun Choi, Cesar Murga-Moreno, Makedonka Mitreva, Miguel M. Cabada
Summary: This study characterized a large sample of Fasciola hepatica with varying triclabendazole susceptibility to determine the complexity of resistance in this parasite. The results showed that resistance varied by geographic location, season, and morphology, providing a basis for further research on the association between resistance phenotype and genotype.
Article
Parasitology
Carolin M. Morawietz, Alejandra M. Peter Ventura, Christoph G. Grevelding, Simone Haeberlein, Bernhard Spengler
Summary: Understanding drug penetration, distribution, and metabolization in parasites is essential for drug efficacy. Recently, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) has been used to visualize the distribution patterns of drug compounds in blood flukes and liver flukes. This technique provides high spatial resolution and has the potential to contribute to preclinical drug discovery in liver fluke research.
PARASITOLOGY RESEARCH
(2022)
Article
Veterinary Sciences
Jane M. Kelley, Grant Rawlin, Travis Beddoe, Mark Stevenson, Terry W. Spithill
Summary: This study in Australia highlighted deficiencies in controlling Fasciola hepatica infection in dairy cattle, such as excessive use of clorsulon, limited rotation of flukicides, lack of diagnostic testing to inform treatment, and potential underdosing of animals. Communication gaps were identified in diagnostic testing for flukicide use, rotation of flukicide actives, flukicide administration, and testing of replacement animals.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Parasitology
Anand Chakroborty, Deiniol Pritchard, Marc E. Bouillon, Anna Cervi, Alan Cookson, Charlotte Wild, Caroline Fenn, Joseph Payne, Peter Holdsworth, Colin Capner, Jenna O'Neill, Gilda Padalino, Josephine Forde-Thomas, Sandeep Gupta, Brendan G. Smith, Maggie Fisher, Martina Lahmann, Mark S. Baird, Karl F. Hoffmann
Summary: Control of liver fluke infections is a significant challenge in the livestock sector due to drug resistant parasites. This study evaluated the anthelmintic activities of chemically modified abietic acid analogues against F. hepatica and found moderate activity in one analogue, but it also showed toxicity to mammalian cells. Therefore, further medicinal chemistry improvements are needed to consider these analogues as potential anthelmintics against liver fluke pathogens.
VETERINARY PARASITOLOGY
(2022)
Article
Parasitology
Monique Costa, Anderson Saravia, Diego Ubios, Pablo Lores, Valeria da Costa, Maria Florencia Festari, Mercedes Landeira, Santiago A. Rodriguez-Zraquia, Georgget Banchero, Teresa Freire
Summary: The aim of this research was to investigate the relationship between hepatic function, hematological parameters, leukocyte counts in circulation, parasite egg shedding, and the effects of triclabendazole (TCZ) treatment during Fasciola hepatica infection in cattle. The results showed increased levels of serum aspartate aminotransferase (AST) and gamma glutamyltransferase (GGT) in the early stages of infection. Additionally, high circulating eosinophil count and plateletcrit levels were correlated with the number of flukes in the livers of infected cattle. TCZ treatment reduced parasite burden and liver damage in chronically infected cattle but did not completely eliminate them.
EXPERIMENTAL PARASITOLOGY
(2022)
Review
Veterinary Sciences
Guilherme Drescher, Tassia Cristina Bello de Vasconcelos, Vinicius Silva Belo, Mariane Marques da Guarda Pinto, Jaqueline de Oliveira Rosa, Luis Gustavo Morello, Fabiano Borges Figueiredo
Summary: Fasciola hepatica can cause problems in both animals and humans. Serological diagnosis of Fasciola is based on recombinant antigens secreted by this worm. The studies found that using native excretory-secretory antigens and recombinant cathepsin in ELISA tests can be effective in serological diagnosis of fascioliasis.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Veronica Molina-Hernandez, Maria T. Ruiz-Campillo, Francisco J. Martinez-Moreno, Leandro Buffoni, Alvaro Martinez-Moreno, Rafael Zafra, Maria J. Bautista, Alejandro Escamilla, Raul Perez-Caballero, Jose Perez
Summary: The study evaluated hepatic lesions in sheep vaccinated with a partly protective vaccine, a non-protective vaccine, and an infected control group. The partially protective vaccine group showed less severe hepatic lesions compared to the infected control group, with dead flukes surrounded by severe granulomatous inflammation observed in the protective vaccine group. This finding suggests that the host response induced by the vaccine may have contributed to the death of adult flukes.
Article
Infectious Diseases
Muslimah N. Alsulami, Khalil Mohamed, Majed H. Wakid, Rewaida Abdel-Gaber, Ashraf G. Timsah, Wafa Abdullah Al-Megrin, Adil Khan, Walaa A. Elkholy, Khaled A. A. Abdelaal, Hatem A. Elshabrawy, Asmaa M. El-Kady
Summary: This study genetically characterized Fasciola adult worms isolated from sheep in Jeddah, Saudi Arabia and found that F. hepatica was the only species isolated in the area. These findings are important for the development of disease control measures.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Parasitology
Zhengjiao Wu, Jinhui Wang, Zhen Meng, Weikun Jin, Kangxin He, Weiyu Zhang, Wenda Di
Summary: This study conducted an in-depth investigation on Fasciola infection in buffaloes in Nanning, Guangxi, China, revealing an infection rate of 38.6% and severe liver damage. A total of 1243 Fasciola flukes were collected from the livers, showing significant morphological variations but lacking accuracy in species identification. Phylogenetic analysis indicated that all flukes were Fasciola gigantica, with no presence of Fasciola hepatica or intermediate forms.
VETERINARY PARASITOLOGY
(2021)
Article
Parasitology
Minhao Zeng, Xiaoxu Wang, Yangyuan Qiu, Xiaojing Sun, Hongyu Qiu, Xiaoxiao Ma, Qingbo Lv, Junfeng Gao, Chunren Wang, Qiaocheng Chang
Summary: Fasciolosis is a neglected parasitic disease caused by liver flukes, with harmful effects on livestock and human health. Changes in host metabolism during F. hepatica infection remain unclear. The study established an artificial sheep model infected with F. hepatica to investigate changes in host metabolic pathways using biochemical parameters and metabolomic analysis. The findings suggest that F. hepatica infection alters the host's metabolic pathways, and the screened biomarkers have the potential for diagnosing the infection.
VETERINARY PARASITOLOGY
(2023)
Article
Parasitology
Jesus Lopez Corrales, Krystyna Cwiklinski, Carolina De Marco Verissimo, Amber Dorey, Richard Lalor, Heather Jewhurst, Amanda McEvoy, Michael Diskin, Catherine Duffy, S. Louise Cosby, Orla M. Keane, John Pius Dalton
Summary: Fasciolosis, a parasitic disease of agricultural livestock caused by the liver fluke Fasciola hepatica, is traditionally diagnosed using laborious methods with low sensitivity. Enzyme linked immunosorbent assays (ELISA) offer a faster and more sensitive alternative for diagnosis, with recombinant cathepsin L proteins showing promise as effective antigens in detecting liver fluke infection in sheep.
VETERINARY PARASITOLOGY
(2021)
Article
Microbiology
David Becerro-Recio, Javier Gonzalez-Miguel, Alberto Ucero, Javier Sotillo, Alvaro Martinez-Moreno, Jose Perez-Arevalo, Krystyna Cwiklinski, John P. Dalton, Mar Siles-Lucas
Summary: This study analyzed the immune response elicited in sheep during an experimental infection by analyzing the excretory/secretory products from adult worms of Fasciola hepatica. Ten different immunogenic proteins from FhES were identified, with some proteins recognized throughout different stages of the infection. Additionally, four FhES proteins were identified for the first time as non-immunogenic proteins, providing insights into host/parasite relationships and potential drug target candidates of F. hepatica.