Article
Biochemistry & Molecular Biology
Warin Rangubpit, Eukote Suwan, Danai Sangthong, Kannika Wongpanit, Roger W. Stich, Prapasiri Pongprayoon, Sathaporn Jittapalapong
Summary: Rhipicephalus (Boophilus) microplus is a tick parasite that affects the cattle industry globally. This study used molecular dynamics simulations to investigate the structure of ligand-free RmGST, a glutathione-S-transferase enzyme in R. (B.) microplus. The findings suggest that RmGST has similar structural stability and catalytic activity to other GSTs. These insights could be valuable for managing acaricide resistance and tick control.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Karishma T. Mody, Bing Zhang, Xun Li, Nicholas L. Fletcher, Dewan T. Akhter, Sandy Jarrett, Jun Zhang, Chengzhong Yu, Kristofer J. Thurecht, Timothy J. Mahony, Neena Mitter
Summary: The study focused on the development of veterinary vaccines against Rhipicephalus microplus using silica vesicles as carriers. The biodistribution of SVs and the Bm86 antigen in a live animal model was investigated over 28 days, showing greater accumulation of SVs at the injection site and distribution of Bm86 in lymph nodes, kidney, and liver.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Parasitology
Brian T. Rich, Donald B. Thomas, Michael T. Longnecker, Douglas R. Tolleson, Jay Angerer, Adalberto A. Perez de Leon, Pete D. Teel
Summary: Surveillance for cattle fever ticks is crucial in preventing the reestablishment of tick vectors causing bovine babesiosis in the U.S. This study aimed to determine if infestation of Rhipicephalus microplus larvae induced detectable changes in fecal chemistry. Near-infrared reflectance spectroscopy was used to analyze fecal samples from infested and non-infested cattle, revealing significant differences in fecal spectra associated with different stages of tick infestation.
VETERINARY PARASITOLOGY
(2022)
Article
Microbiology
Patricia Paulino, Gabriela Vitari, Antonio Rezende, Joana Couto, Sandra Antunes, Ana Domingos, Maristela Peckle, Carlos Massard, Flavio Araujo, Huarrisson Santos
Summary: This study characterized the sialotranscriptome profile of Rhipicephalus (Boophilus) microplus in response to Theileria equi and identified genes with differential genomic expression. The analysis revealed potential targets in the tick-protozoan interactions, providing valuable information for future studies and biotechnological applications.
Article
Biochemistry & Molecular Biology
Warin Rangubpit, Eukote Suwan, Danai Sangthong, Kannika Wongpanit, Roger W. Stich, Prapasiri Pongprayoon, Sathaporn Jittapalapong
Summary: In this study, molecular dynamics simulations were conducted to investigate the binding of GTX and GSH to RmGST. It was found that GSH binds tighter and sits rigidly inside the G-site, while GTX occupies both active sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Alejandro Gonzalez-Mora, Kenny Misael Calvillo-Rodriguez, Jesus Hernandez-Perez, Marco Rito-Palomares, Ana Carolina Martinez-Torres, Jorge Benavides
Summary: The study developed a candidate phage-based vaccine displaying a cattle tick antigen, which induced both humoral and cellular immune-specific responses in bovine and murine models. This represents a promising alternative for controlling cattle tick infestations, though further evaluation in a bovine model is necessary.
Article
Parasitology
Juan P. Wulff, Kevin B. Temeyer, Jason P. Tidwell, Kristie G. Schlechte, Caixing Xiong, Kimberly H. Lohmeyer, Patricia Pietrantonio
Summary: This study found that silencing the pyrokinin receptor (Rhimi-PKR) in R. microplus ticks negatively affected female reproductive fitness, including increased mortality and decreased weight.
PARASITES & VECTORS
(2022)
Article
Microbiology
Thais Almeida Correa, Jessica Fiorotti, Emily Mesquita, Laura Nobrega Meirelles, Mariana Guedes Camargo, Caio Junior Balduino Coutinho-Rodrigues, Allan Felipe Marciano, Vania Rita Elias Pinheiro Bittencourt, Patricia Silva Golo
Summary: The study found that exogenous dopamine can increase the survival rate and circulating hemocyte numbers of Rhipicephalus microplus inoculated with Metarhizium anisopliae blastospores. However, exogenous dopamine does not affect the phagocytic ability of tick hemocytes. Additionally, phenoloxidase activity in ticks injected with dopamine is significantly increased, indicating a potential role in the immune response. These findings help improve strategies for the biological control of ticks.
Article
Multidisciplinary Sciences
Jesus Rojas-Jaimes, David Lindo-Seminario, German Correa-Nunez, Benoit Diringer
Summary: The microbiome of ticks collected from collared peccaries in the Amazon region of Madre de Dios, Peru, was characterized for the first time, revealing the presence of potentially pathogenic bacterial genera such as Salmonella and Vibrio. These findings are important for understanding the pathogenic potential of R. microplus and the potential implications of these bacteria for public and animal health.
SCIENTIFIC REPORTS
(2021)
Article
Entomology
Michael T. Suderman, Kevin B. Temeyer, Kristie G. Schlechte, Adalberto A. Perez de Leon
Summary: Ticks feed on blood and transmit diseases, and tick cell culture in 3-D form can provide insights into tick biology and aid in developing control methods. This study successfully adapted 3-D culture technology to cells from the southern cattle fever tick, showing potential applications in advancing tick research and management.
Article
Veterinary Sciences
Remil L. Galay, Carina R. Llaneta, Maria Karla Faye B. Monreal, Antero L. Armero, Arianne Bel D. Baluyut, Czarina Marie F. Regino, Kristina Andrea C. Sandalo, Billy P. Divina, Melbourne R. Talactac, Lennox P. Tapawan, Maarten Czar L. Mojares, Cherry R. Alvarez, Emmanuel R. Mago, Noemi D. Encarnacion, Masako Andoh, Tetsuya Tanaka
Summary: The study investigated the prevalence of Anaplasma marginale and Ehrlichia in cattle and water buffalo from provinces in the southern part of Luzon, Philippines. It found a high prevalence of A. marginale in the region and provided the first molecular evidence of E. minasensis in the country.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Parasitology
Xue-Ling Zhang, Yuan-Ping Deng, Tian Yang, Le-Yan Li, Tian-Yin Cheng, Guo-Hua Liu, De-Yong Duan
Summary: This study characterized the midgut microbiome of Rhipicephalus microplus ticks from China using metagenomic methods. The results showed that the midgut microbiome consists not only of bacteria but also eukaryotes and viruses. The gene functions of the midgut microflora were found to be involved in various metabolic pathways and infectious diseases.
PARASITES & VECTORS
(2022)
Article
Biotechnology & Applied Microbiology
Isabella Barboza de Almeida, Fernanda Calvo Duarte, Luara Lucena Cassiano, Leonardo Costa Fiorini, Lucas Mello Moran, Ana Maria Cristina R. F. Martins, Jose Eduardo marcodes de Almeida, Marcia Cristina Mendes
Summary: The study evaluated the effectiveness of the entomopathogenic fungus Metarhizium anisopliae in controlling Rhipicephalus microplus, indicating stable and long-lasting results in controlling the tick population.
BIOLOGICAL CONTROL
(2022)
Article
Agriculture, Dairy & Animal Science
Sana Ayub, Nosheen Malak, Raquel Cossio-Bayugar, Nasreen Nasreen, Afshan Khan, Sadaf Niaz, Adil Khan, Abdallah D. Alanazi, Mourad Ben Said
Summary: A recent study explored the effectiveness of Pinus roxburghii plant extract in controlling populations of Rhipicephalus (Boophilus) microplus ticks. The study found that the plant extract showed high efficacy in vitro against different tick stages and demonstrated strong binding interaction between the plant's phytochemicals and the tick protein. This suggests the potential use of P. roxburghii as a promising anti-tick agent.
Article
Biochemistry & Molecular Biology
Kornelia Bodo, Zoltan Kellermayer, Zoltan Laszlo, Akos Boros, Bohdana Kokhanyuk, Peter Nemeth, Peter Engelmann
Summary: The study aimed to investigate the mechanisms of innate immunity during regeneration in Eisenia andrei earthworms, revealing dynamic changes in cell proliferation during anterior and posterior regeneration, accumulation of different immune cell subsets in the newly-formed blastema, and downregulation of pattern recognition receptors and antimicrobial peptides mRNA patterns in regenerating earthworms. Experimental coelomocyte depletion significantly impaired cell divisions and blastema formation during regeneration, indicating complex interactions between regeneration and invertebrate innate immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Arielly Oliveira Garcia, Pamela Itajara Otto, Luiz Afonso Glatzl Junior, Renata de Fatima Bretanha Rocha, Mateus Guimaraes dos Santos, Daniele Alves de Oliveira, Marcos Vinicius Gualberto Barbosa da Silva, Joao Claudio do Carmo Panetto, Marco Antonio Machado, Rui da Silva Verneque, Simone Eliza Facioni Guimaraes
Summary: Our objective was to establish a SNP panel for pedigree reconstruction using microarrays of different densities and evaluate the genomic relationship coefficient of the inferred pedigree, in addition to analyzing the population structure based on genomic analyses in Gir cattle. We used genotyped Gir animals and common markers to reconstructed the pedigree and analyzed linkage disequilibrium, effective population size, and runs of homozygosity. The findings suggest the need for breeding strategies to minimize inbreeding and increase effective population size to maintain genetic variability in future generations.
JOURNAL OF APPLIED GENETICS
(2023)
Article
Biodiversity Conservation
Tuliana O. Brunes, Felipe C. S. Pinto, Pedro P. G. Taucce, Marcus Thadeu T. Santos, Luciana B. Nascimento, Daniel C. Carvalho, Guilherme Oliveira, Santelmo Vasconcelos, Felipe S. F. Leite
Summary: Despite the difference in land coverage between mountains and lowlands, most species are found in mountains and foothills, which has been a challenge for biogeographers and evolutionary biologists.
SYSTEMATICS AND BIODIVERSITY
(2023)
Article
Agriculture, Dairy & Animal Science
Leticia Fernanda de Oliveira, Paulo Savio Lopes, Layla Cristien Cassia Miranda Dias, Leandro Magno Dornelas e Silva, Hugo Teixeira Silva, Simone Eliza Facioni Guimaraes, Daniele Botelho Diniz Marques, Delvan Alves da Silva, Renata Veroneze
Summary: This study estimated genetic parameters, effective population size, inbreeding, and inbreeding depression in Piau pigs. The results showed low direct heritabilities for birth weight and weaning weight, while average pre-weaning daily weight gain showed moderate heritability. There were high genetic correlations between weight at birth and weight at weaning, as well as weight at weaning and average pre-weaning daily weight gain. Inbreeding increased over generations and led to a reduction in effective population size. Inbreeding had a significant effect on average pre-weaning daily weight gain, with a decrease of 0.005 g for every 1% increase in inbreeding coefficient. Increasing effective population size is necessary to control inbreeding and preserve genetic variability in the Piau pig population.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2023)
Article
Agriculture, Dairy & Animal Science
Elisa Peripolli, Nedenia Bonvino Stafuzza, Marco Antonio Machado, Joao Claudio do Carmo Panetto, Andrea Alves do Egito, Fernando Baldi, Marcos Vinicius Gualberto Barbosa da Silva
Summary: Further characterization of genetic structural variations should focus on small and endangered local breeds. A comprehensive genome-wide assessment of copy number variations (CNVs) in Brazilian cattle breeds revealed important genes and variants. The CNVR distribution is non-uniform and strongly associated with immunity and cattle resilience to harsh environments. QTLs associated with body conformation and dairy-related traits were also identified.
Article
Biochemistry & Molecular Biology
Renata de Fatima Bretanha Rocha, Arielly Oliveira Garcia, Pamela Itajara Otto, Marcos Vinicius Barbosa da Silva, Marta Fonseca Martins, Marco Antonio Machado, Joao Claudio do Carmo Panetto, Simone Eliza Facioni Guimaraes
Summary: The study aimed to verify the impact of ROH and inbreeding depression on total oocytes and embryos in Gir Indicine cattle, and to identify genes and enriched regions related to these traits. Generally, an increase in ROH was found to decrease the number of total oocytes and viable embryos. Additionally, multiple genes and genomic regions were identified as being associated with different breeding values for these traits.
Article
Multidisciplinary Sciences
Larissa G. Braga, Tatiane C. S. Chud, Rafael N. Watanabe, Rodrigo P. Savegnago, Thomaz M. Sena, Adriana S. do Carmo, Marco A. Machado, Joao Claudio do C. Panetto, Marcos Vinicius G. B. da Silva, Danisio P. Munari
Summary: This study investigated the genome of Dairy Gir cattle breed and identified and characterized high confidence copy number variation regions (CNVR). A total of 10 CNVR_POP and 45 CNVR_ANI were found, which are associated with genes related to complex traits. These findings contribute to a better understanding of the Gir breed genome and provide insights into genes associated with complex traits such as production, health, and reproduction.
Article
Multidisciplinary Sciences
Rafael de Fraga, Valeria Tavares, Matheus Henrique Simoes, Xavier Prous, Cesare Girolamo-Neto, Iuri V. Brandi, Guilherme Oliveira, Leonardo C. Trevelin
Summary: This paper examines the impact of habitat degradation on the richness and composition of animal communities inside caves. The study shows that caves can serve as refuges for fauna when the native vegetation cover surrounding them is degraded. The findings highlight the importance of protecting surface habitats for the conservation of cave ecosystems.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Renata de Fatima Bretanha Rocha, Arielly Oliveira Garcia, Pamela Itajara Otto, Mateus Guimaraes dos Santos, Marcos Vinicius Barbosa da Silva, Marta Fonseca Martins, Marco Antonio Machado, Joao Claudio do Carmo Panetto, Simone Eliza Facioni Guimaraes
Summary: This study used GWAS to identify genomic regions, genes, and biological processes associated with the number of embryos and oocytes in Gir dairy cattle. Several protein-coding genes were found to be related to embryo development and cell functions.
Article
Agriculture, Dairy & Animal Science
Clara Slade Oliveira, Luiz Sergio Almeida Camargo, Marcos Vinicius Gualberto Barbosa da Silva, Naiara Zoccal Saraiva, Carolina Capobiango Quintao, Marco Antonio Machado
Summary: Genomic selection has revolutionized the livestock industry by allowing early-life selection of animals. Biopsy sampling of pre-implantation embryos has been available since 1968, but it was only with the development of molecular biology techniques that next-generation sequencing became commercially feasible for bovine embryos after 2010. This technology enables decision-making on embryo transfer based not only on morphological characteristics but also on genomic estimates, with a wide range of applications in livestock.
ANIMAL REPRODUCTION
(2023)
Article
Developmental Biology
Arthur Nery da Silva, Adriana Mercia Guaratini Ibelli, Igor Ricardo Savoldi, Mauricio Egidio Cantao, Eraldo Lourenso Zanella, Mariana Groke Marques, Marcos Vinicius Gualberto Barbosa da Silva, Jane Oliveira de Peixoto, Monica Correa Ledur, Jader Silva Lopes, Jose Eduardo Vargas, Ricardo Zanella
Summary: Cryptorchidism is a hereditary anomaly that affects animal physiology, leading to reduced welfare and economic losses. This study identified new single nucleotide polymorphisms and genes associated with cryptorchidism in pigs using whole-exome sequencing. Functional pathway analysis revealed the involvement of estrogen signaling, cytoskeleton organization, and the pentose phosphate pathway.
SEXUAL DEVELOPMENT
(2023)
Article
Multidisciplinary Sciences
Breno Hamdan, Victor C. Seixas, Gisele L. Nunes, Guilherme Oliveira, Sandro L. Bonatto, Amanda Vidal, Eder S. Pires, Russolina B. Zingali
Summary: This study presents the complete mitogenome of the Brazilian Atlantic bushmaster for the first time and provides insights into snake evolution. Phylogenetic analysis reveals the evolutionary relationship of the Brazilian Atlantic bushmaster with other snake species. Additionally, the study analyzes the genetic distance between different snake families, revealing the genetic variations among snake species.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2023)
Article
Agriculture, Dairy & Animal Science
Margareth Evangelista Botelho, Marcos Soares Lopes, Pramod K. Mathur, Egbert F. Knol, Daniele B. D. Marques, Paulo Savio Lopes, Fabyano Fonseca e Silva, Simone Eliza Facioni Guimaraes, Renata Veroneze
Summary: This study aimed to investigate the causal relationship and causal effects among boar taint compounds measured in pig adipose tissue from carcasses and biopsies. The results showed that boar taint compounds measured in biopsies have direct effects on the compounds measured in carcasses.
ANIMAL PRODUCTION SCIENCE
(2023)
