Article
Parasitology
Hany M. Ibrahim, Eloiza May S. Galon, Maria Agnes Tumwebaze, Benedicto Byamukama, Mingming Liu, Khaled Mohammed-Geba, Sherin K. Sheir, Asmaa Galal-Khallaf, Heba M. Abd El Latif, Dalia S. Morsi, Nora M. Bishr, Xuenan Xuan
Summary: The study aimed to determine the seroprevalence of Babesia bigemina and B. bovis in cattle and water buffaloes in Menoufia province, Egypt. The results showed that babesiosis is frequent in the province, posing a potential threat to milk and meat production in the area.
ACTA PARASITOLOGICA
(2021)
Article
Parasitology
Sabrina Ganzinelli, Charles Byaruhanga, Maria E. Primo, Zinathi Lukanji, Kgomotso Sibeko, Tshepo Matjila, Luis Neves, Daniel Benitez, Batmagnai Enkhbaatar, Arifin Budiman Nugraha, Ikuo Igarashi, Monica Florin-Christensen, Leonhard Schnittger
Summary: Babesia bovis and B. bigemina are tick-transmitted parasites that cause bovine babesiosis, leading to economic losses in the livestock industry. This study developed improved detection methods with high sensitivity for these parasites and compared them to reference protocols, demonstrating their superior capability in detecting carrier animals. Additionally, the reproducibility of the new methods was validated in different laboratories.
VETERINARY PARASITOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Mao Huang, Yanwen Yin, Kaichuang Shi, Hongtao Zhang, Xinru Cao, Xingju Song
Summary: This study investigated the seroprevalence of Neospora caninum infection in water buffaloes and dogs in China, and found a high prevalence in both species. These findings are important for the prevention and control of the disease.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Microbiology
Ian Cary B. Prado, Larry Xerxes B. Capuno, Princess DLP. Collera, Aaron Paul D. Cabralda, Kristina Andrea S. De Ramos, John Michael G. Bernardo, Billy P. Divina, Tatsunori Masatani, Tetsuya Tanaka, Remil L. Galay
Summary: Tick-borne protozoan parasites Babesia and Theileria can cause economic losses in the cattle industry. This study investigated the presence of these parasites in large ruminants in the Philippines, confirming the prevalence of T. orientalis and reporting the genetic diversity of B. bovis and T. orientalis.
Article
Veterinary Sciences
Carolina Beatriz Baptista, Matheus Janeck Araujo, Sandra Valeria Inacio, Bruno Criado de Araujo Mendes, Monally Conceica Costa de Aquino, Elis Domingos Ferrari, Katia Denise Saraiva Bresciani, Alvimar Jose da Costa
Summary: This study reports, for the first time, the occurrence of G. duodenalis assemblage A in pet rabbits in Brazil. PCR analysis of fecal samples from 100 rabbits revealed 40 positive cases. This research is important for understanding parasite infections in Brazilian rabbits.
PREVENTIVE VETERINARY MEDICINE
(2023)
Article
Microbiology
Jose Juan Lira-Amaya, Rebeca Montserrat Santamaria-Espinosa, Roberto O. Castaneda-Arriola, Grecia Martinez-Garcia, Diego J. Polanco-Martinez, Carmen Rojas-Martinez, Jesus Antonio Alvarez-Martinez, Julio Figueroa-Millan
Summary: This study investigated the presence of Babesia and Anaplasma spp. sequences in water buffalo blood samples collected from farms in Veracruz and Tabasco state, Mexico. The results confirmed that water buffalo can act as carriers of hemoparasite infections, which are tick-transmitted and may play an important role in the epidemiology of bovine babesiosis in Mexico.
Article
Agriculture, Multidisciplinary
Asif Masih, Azhar Rafique, Farhat Jabeen, Shabana Naz
Summary: This study aimed to detect Babesia ovis in sheep and goat herds in Pakistan using microscopy and molecular diagnostic techniques. The results showed that nPCR had higher sensitivity compared to conventional PCR, and different risk factors could affect the incidence of the disease.
PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES
(2022)
Article
Microbiology
Guangpu Yang, Bingqian Zhou, Kewei Chen, Zhe Hu, Wei Guo, Xiaojun Wang, Cheng Du
Summary: Novel competitive ELISA and western blot assays were developed to detect antibodies against T. equi or B. caballi. These methods have high sensitivity, no cross-reactivity with other equine diseases, and showed high coincidence rates compared to commercially available kits.
Review
Parasitology
El-Sayed El-Alfy, Ibrahim Abbas, Rana Elseadawy, Somaya Saleh, Bassem Elmishmishy, Shimaa Abd El-Salam El-Sayed, Mohamed Abdo Rizk
Summary: Background buffaloes are economically important in many countries, especially in Asia, but they are commonly infected by tick-borne pathogens (TBPs) that can cause serious diseases. This investigation aimed to determine the prevalence of TBPs infecting buffaloes worldwide. Global data on TBPs in buffaloes were collected and analyzed using meta-analysis, revealing a high species diversity and highlighting the economic implications for the livestock industries.
PARASITES & VECTORS
(2023)
Article
Agriculture, Dairy & Animal Science
Qin Liu, Xueyan Zhan, Dongfang Li, Junlong Zhao, Haiyong Wei, Heba Alzan, Lan He
Summary: A novel BgGPI52-WH antigen was identified as a potential immunodiagnostic marker and iELISA method was established for early diagnosis with good sensitivity. Clinical samples showed a positive rate of approximately 11.41%.
Article
Immunology
Abdelfattah Selim, Ayed Alshammari, Hattan S. Gattan, Mohammed H. Alruhaili, Gehan A. Rashed, Salma Shoulah
Summary: This study investigated the seroprevalence of Toxoplasma gondii infection in water buffaloes in Egypt and found that age and herd size of the buffaloes were associated with the infection risk. Additionally, winter season and contact with cats were identified as higher risk factors for the buffaloes to contract T. gondii infection. It is important to identify and address these risk factors to reduce and control the infection in buffaloes and subsequently decrease the risk of human infection.
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2023)
Article
Agriculture, Dairy & Animal Science
Qudratullah, Ghulam Muhammad, Tariq Jamil, Imaad Rashid, Qudrat Ullah, Muhammad Saqib
Summary: The study evaluated the efficacy of a locally prepared hemorrhagic septicemia and mastitis combined vaccine, showing significant immunogenic effects in dairy cows and buffaloes, including higher antibody titers, lower somatic cell counts and mastitis severity scores, as well as higher milk yield. Challenge studies are still needed to further validate the results.
Article
Agriculture, Dairy & Animal Science
Lucianne Cardoso Neves, Lucas Christian de Sousa-Paula, Sarah Alves Dias, Bianca Barbara Fonseca da Silva, Warley Vieira de Freitas Paula, Luiza Gabriella Ferreira de Paula, Brenda Gomes Pereira, Gracielle Teles Padua, Ana Carolina Borsanelli, Ennya Rafaella Neves Cardoso, Felipe da Silva Krawczak, Filipe Dantas-Torres
Summary: We assessed the presence of piroplasmids and Ehrlichia spp. in capybaras and their associated ticks in Goias state, central-western Brazil. Neither capybaras nor ticks were positive for Ehrlichia spp. However, we detected an undescribed species of protozoan in both the capybaras and ticks.
Article
Microbiology
Marcos Rogerio Andre, Ana Claudia Calchi, Maria Eduarda Chiaradia Furquim, Isabela de Andrade, Paulo Vitor Cadina Arantes, Lara Cristina de Melo Lopes, Iuri Kauan Lins do Nascimento Demarchi, Mayra Araguaia Pereira Figueiredo, Cirilo Antonio de Paula Lima, Rosangela Zacarias Machado
Summary: Despite extensive research on tick-borne agents (TBA) in dogs, little is known about the occurrence, vectors, and molecular identity of these agents in cats. This study aimed to investigate the presence and molecular identity of TBA in cats from different regions in Brazil. The results showed the presence of various TBA in cats, emphasizing the importance of awareness and proper diagnosis among veterinary practitioners when dealing with cats infected with multiple TBA.
Article
Parasitology
Lavanya K. Vijaykumar, Puttalakshmamma G. Chikkachowdappa, Mohan H. Venkatappa, Apsana Rizvan, Revanaiah Yogisharadhya, Sathish Bhadravati Shivachandra, Manjunatha Reddy G. Bayyappa
Summary: Canine babesiosis, a tick-borne haemopmtozoan disease of dogs, is rapidly spreading globally, emphasizing the need for precise diagnosis. The study evaluated a recombinant BgSA3 protein based indirect ELISA for sero-diagnosis and semi-surveillance of Babesia gibsoni infection in dogs. The method showed high specificity and sensitivity, making it suitable for large-scale surveillance of B. gibsoni infection.
VETERINARY PARASITOLOGY
(2021)
Article
Agronomy
Jessica Cecilia Pinheiro Rodrigues, Isabela de Souza Parry, Thais de Cassia Mouzinho Lopes, Eder Barros dos Santos, Livia Caricio Martins, Adivaldo Henrique da Fonseca, Paulo Cesar Magalhaes-Matos
Summary: In the Iguacu National Park, some free-living coatis have antibodies against different types of arboviruses, indicating a potential transmission cycle in the area.
Article
Biochemical Research Methods
Hongxia Bai, Leonard B. B. Collins, Marcos Rogerio Andre, Edward B. B. Breitschwerdt, Taufika Islam Williams
Summary: A bottom-up proteomics workflow was developed to study a system containing multiple organisms, allowing a thorough understanding of their interactions. The workflow utilized label-free quantification proteomics with nanoLC-MS/MS. Detailed instructions were provided to promote the adoption of this workflow in other laboratories.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Article
Veterinary Sciences
Claudia Almeida Scariot, Julian Scariot, Inalda Angelica de Souza Ramos, Luiz Ricardo Gonsalves, Ana Claudia Calchi, Marcos Rogerio Andre, Rosangela Zacarias Machado, Marcio Machado Costa, Luiz Carlos Kreutz, Ricardo Zanella, Maria Isabel Botelho Vieira
Summary: This study aimed to evaluate the occurrence of A. marginale in dairy cattle using qPCR and assess its association with clinical cases of retained placenta, mastitis, and abomasal displacement. The results showed that cows infected with A. marginale had a higher risk of developing retained placenta and mastitis, which negatively impacted their productivity.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Parasitology
agatha Ferreira Xavier de Oliveira, Ana Claudia Calchi, Anieli Vidal Stocco, Naiara Vidal Stocco, Alexandre Carvalho Costa, Elisabeth Neves Mureb, Jeferson Rocha Pires, Andresa Guimaraes, Juliana Macedo Raimundo, Daniel de Almeida Balthazar, Rosangela Zacarias Machado, Marcos Rogerio Andre, Cristiane Divan Baldani
Summary: The aim of the study was to investigate the presence of piroplasmids in opossums rescued from the metropolitan region of Rio de Janeiro state, Brazil. Five out of fifteen opossums tested positive for piroplasms, with two animals showing intra-erythrocytic structures. One infected animal exhibited clinical symptoms of infection. The study proposes a new Piroplasmida Clade, known as the South American Marsupialia Group.
PARASITOLOGY RESEARCH
(2023)
Article
Microbiology
Livia Perles, Wanessa Teixeira Gomes Barreto, Filipe Martins Santos, Leidiane Lima Duarte, Gabriel Carvalho de Macedo, Darci Moraes Barros-Battesti, Heitor Miraglia Herrera, Rosangela Zacarias Machado, Marcos Rogerio Andre
Summary: The present study aimed to detect hemotropic Mycoplasma spp. and Bartonella spp. in coatis from forested urban areas in midwestern Brazil. Two different hemoplasma genotypes were detected in coatis' blood samples, with 71% positive for myc1 and 17% positive for myc2. No Bartonella sp. was detected in coatis, but positive results were found in tick samples.
Article
Microbiology
Charlotte Moore, Erin Lashnits, Pradeep Neupane, Brian H. H. Herrin, Michael Lappin, Marcos Rogerio Andre, Edward B. B. Breitschwerdt
Summary: The effect of Bartonella henselae on the microbiome of Ctenocephalides felis (the cat flea) was investigated in this study. The microbiome of laboratory-origin C. felis fed on B. henselae-infected cats for 24 h or 9 days was analyzed to identify changes in microbial diversity. It was found that microbial diversity increased after 24 h of feeding on infected cats but returned to baseline after 9 days. B. henselae acquisition was poor, with only one out of four infected flea pools showing detection.
Article
Public, Environmental & Occupational Health
Paulina Sepulveda-Garcia, Amir Alabi, Karla Alvarez, Lisbeth Rojas, Armin Mella, Luiz Ricardo Goncalves, Marcos Rogerio Andre, Rosangela Zacarias Machado, Ananda Muller, Gustavo Monti
Summary: The aim of this study was to estimate the occurrence of Bartonella spp. in cats and their owners in Valdivia, Chile. The study found that 20.43% of households had at least one Bartonella positive cat, but Bartonella DNA was not found in the blood samples of humans. Risk factors for Bartonella spp. presence in cats included age, neutering status, sampling location, and improper tick/flea control products.
Article
Microbiology
Felipe da Silva Krawczak, Ana Claudia Calchi, Lucianne Cardoso Neves, Sarah Alves Dias, Bianca Barbara Fonseca da Silva, Warley Vieira de Freitas Paula, Luiza Gabriella Ferreira de Paula, Mariana Avelar Tavares, Gracielle Teles Padua, Nicolas Jalowitzki de Lima, Ennya Rafaella Neves Cardoso, Daniel Graziani, Filipe Dantas-Torres, Marcos Rogerio Andre
Summary: Novel Piroplasmida clades and species were found in wild animals from Brazil. A putative new Babesia species was detected in capybaras and associated ticks through phylogenetic assessments based on distinct molecular markers. The phylogenetic analysis showed that this new species belonged to a new clade sister to the Babesia sensu stricto clade and the Theileria sensu stricto clade.
Article
Microbiology
Ana Carolina Castro-Santiago, Leidiane Lima-Duarte, Jaqueline Valeria Camargo, Beatriz Rocha De Almeida, Simone Michaela Simons, Luis Antonio Mathias, Ricardo Bassini-Silva, Rosangela Zacarias Machado, Marcos Rogerio Andre, Darci Moraes Barros-Battesti
Summary: This study aimed to investigate the ability of O. fonsecai and O. brasiliensis nymphs to acquire, infect, and perpetuate Anaplasma marginale through artificial feeding. The DNA of A. marginale was detected in nymphs of both tick species after molting and artificial feeding.
Article
Parasitology
Livia Perles, Wanessa Teixeira Gomes Barreto, Gabriel Carvalho de Macedo, Heitor Miraglia Herrera, Rosangela Zacarias Machado, Marcos Rogerio Andre
Summary: This study investigated the occurrence of Neorickettsia sp. in blood samples from coatis in central-western Brazil. Nested PCR targeting the 16S rRNA gene detected 6 positive samples with >99% similarity to N. risticii. This is the first molecular detection of Neorickettsia sp. in coatis in Brazil.
REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA
(2023)
Article
Parasitology
Thiago Merighi Vieira da Silva, Mariele De Santi, Luiz Ricardo Goncalves, Marcia Mariza Jusi Merino, Marcos Rogerio Andre, Rosangela Zacarias Machado
Summary: In Brazil, 69.88% of the 342 horses sampled were found to have Equine Protozoal Myeloencephalitis (EPM) caused by Sarcocystis neurona, while 51.75% of the horses were found to have EPM caused by Sarcocystis falcatula-like (Dal-CG23). Furthermore, 38.59% of the horses showed antibody reactions to both isolates.
REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA
(2023)
Article
Veterinary Sciences
N. R. N. Cruz, M. R. Andre, T. G. Baraldi, L. A. Mathias, L. A. N. Braz, L. G. Oliveira, A. E. Santana
Summary: This study investigated the molecular occurrence of porcine hemoplasmas in technified swine herds in Southeastern Brazil and found that the overall prevalence of hemoplasmosis was 31.93%. The prevalence varied among different growth stages of the pigs.
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
(2023)
Article
Immunology
Alvaro A. Faccini-Martinez, Louise Bach Kmetiuk, Lucas S. Blanton, Lais Giuliane Felipetto, Mara Lucia Gravinatti, Jorge Timenetsky, Luiz Ricardo Goncalves, Rosangela Zacarias Machado, Marcos Rogerio Andre, Fabiano Borges Figueiredo, Andrea Pires dos Santos, Marcelo B. Labruna, Gustavo Monti, Alexander Welker Biondo, David H. Walker
Summary: Homeless people in Sao Paulo, Brazil, were found to have high rates of Bartonella spp. and typhus group rickettsiae. Bartonella quintana DNA was detected in body lice pools and blood samples. Clinicians should consider vectorborne agents as potential causes of febrile syndromes in this population.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Entomology
Victor de Souza Mello-Oliveira, Fernando de Castro Jacinavicius, Mariane Guerro-de-Souza, Leormando Fortunato Dornelas Jr, Ana Carolina Castro-Santiago, Ana Claudia Calchi, Elizabete Captivo Lourenco, Ashley P. G. Dowling, Katia Maria Famadas, Marcos Rogerio Andre, Luis Marcelo Aranha Camargo, Darci Moraes Barros-Battesti, Ricardo Bassini-Silva
Summary: This study identified and provided diagnostic microscopic images and molecular information of Parichoronyssus bakeri, a macronyssid mite collected from bats. New locality records for P. bakeri in Rio de Janeiro and Rondonia States, Brazil were also reported.
Article
Ecology
L. Perles, D. Otranto, W. T. G. Barreto, G. C. de Macedo, R. P. Lia, J. A. Mendoza-Roldan, H. M. Herrera, C. E. de Oliveira, R. Z. Machado, M. R. Andre
Summary: Coatis are wild carnivores well adapted to human-disturbed environments and serve as reservoir hosts for arthropod-borne zoonotic pathogens. A study in Brazil revealed a high prevalence of filarial nematodes in coatis, suggesting their role as potential hosts for a novel species within the Onchocercidae family.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE
(2023)
