Article
Parasitology
Carlos Jose Raupp Ramos, Cintia de Souza Franco, Sabrina Pogere da Luz, Julia Marques, Ketriane Mota de Souza, Luiz Flavio Nepomuceno do Nascimento, Gabriella Bassi das Neves, Renato Simoes Moreira, Luiz Claudio Miletti
Summary: This study reports, for the first time, the presence of Trypanosoma evansi DNA in the feeding apparatus of Dichelacera alcicornis and Dichelacera januarii from South America.
PARASITES & VECTORS
(2023)
Article
Agriculture, Dairy & Animal Science
Dina M. Metwally, Isra M. Al-Turaiki, Najwa Altwaijry, Samia Q. Alghamdi, Abdullah D. Alanazi
Summary: The study investigated the occurrence of Trypanosoma evansi in camels in Saudi Arabia and found that nearly half of the camels were infected, despite being undetectable in thin blood smears. The infection causes significant financial burden to camel breeders and owners, and detection of the parasite can reduce financial losses and improve camel mortality. Polymerase chain reaction was found to be more effective than microscopy at identifying T. evansi infection in camels.
Article
Chemistry, Analytical
Jiarui Zhang, Rong Zhao, Kejun Dong, Wan Shu, Sitian Wei, Yuwei Yao, Jun Zhang, Shuangge Liu, Wei Zhang, Hongbo Wang
Summary: DNA damage is a major cause of diseases, and UDG plays a crucial role in repairing DNA damage and serves as an early diagnostic marker and prognostic indicator for cancer. To meet the clinical demand, a simple, low-background, ultra-sensitive UDG activity detection method is urgently needed.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Ophthalmology
Douglas A. Jabs, Nisha R. Acharya, Laure Caspers, Soon-Phaik Chee, Debra Goldstein, Peter McCluskey, Philip Murray, Neal Oden, Alan G. Palestine, James T. Rosenbaum, Jennifer E. Thorne, Brett E. Trusko
Summary: This study aimed to determine classification criteria for herpes simplex virus anterior uveitis using machine learning. Results showed that the criteria had a low misclassification rate and performed well in both the training and validation sets, suggesting their potential use in clinical and translational research.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2021)
Article
Multidisciplinary Sciences
Nupur Garg, Upasana Sahu, Sudeshna Kar, Farhan J. Ahmad
Summary: This study introduces a fast, sensitive, and specific LAMP technique for early detection of leprosy, which showed complete concurrence with conventional techniques in blind clinical validation studies. Visual detection methods were also standardized for easy-to-read and rapid diagnosis, eliminating the lack of accuracy and sensitivity in skin smear tests and the need for expensive lab equipments and trained technicians.
SCIENTIFIC REPORTS
(2021)
Article
Public, Environmental & Occupational Health
Natasha S. Hochberg, Alyse Wheelock, Davidson H. Hamer, Rachel Marcus, Melissa S. Nolan, Sheba Meymandi, Robert H. Gilman
Summary: Chagas disease, a neglected tropical disease affecting an estimated 300,000 people in the United States, poses diagnostic challenges that need to be addressed. This perspective piece reviews these challenges and proposes next steps for improvement.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Nanoscience & Nanotechnology
Shun-ichi Matsuura, Tomoya Baba, Takuji Ikeda, Katsutoshi Yamamoto, Tatsuo Tsunoda, Aritomo Yamaguchi
Summary: The mesoporous silica-enhanced PCR technology allows highly precise and sensitive DNA amplification/detection from minimal amounts of nucleic acid, making it applicable for genetic testing involving small samples.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Chemistry, Analytical
Xinran Dong, Yuchen Huang, Dechen Jiang
Summary: A fluorescent polymerase chain reaction (PCR) nanokit was established for detecting specific DNA sequences in single living cells. This strategy involves sorting the DNA sequence in a cellular nucleus into a nanopipette, where it reacts with PCR kit components to complete a PCR amplification reaction. The presence of the target DNA sequence is validated through an increase in fluorescence, and the nanokit offers a general and highly sensitive approach for single cell analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Thermodynamics
Hassan Ali Abid, Jian Wern Ong, Zhixiong Song, Eric Shen Lin, Oi Wah Liew, Tuck Wah Ng
Summary: PCR amplifies specific DNA targets through precise temperature control and efficient heat transfer. Tilt method can achieve uniform temperature distributions and shorter settling times, potentially enhancing the efficiency of DNA amplification.
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
(2021)
Article
Pathology
Cecile Nabet, Jean-Yves Brossas, Corentin Poignon, Amira Bouzidi, Luc Paris, Feriel Touafek, Emmanuelle Varlet-Marie, Yvon Sterkers, Karine Passebosc-Faure, Marie-Laure Darde, Renaud Piarroux, Jerome Alexandre Denis
Summary: Accurate detection and quantification tools for Toxoplasma gondii are valuable for the management of toxoplasmosis. This study evaluated the performance of droplet digital PCR (ddPCR) for T. gondii detection and quantification. The results showed that ddPCR had equivalent performance to qPCR for T. gondii detection and was more accurate for quantification.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2023)
Article
Multidisciplinary Sciences
Jae Jong Kim, Hyoung-Min Park, A. Young Kyoung, In Kyung Park, Si-Kyu Lim, Byoung Chul Park
Summary: Genetic mutations like SNP are common in genetic disorders and cancers, and PCR methods are widely used for SNP detection. The specifically designed PCR platform STexS can efficiently distinguish small amounts of SNP mixed in normal DNA, aiding in targeting potential SNPs or genetically mutated biomarkers in human clinical samples.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Renna L. Nouwairi, Larissa L. Cunha, Rachelle Turiello, Orion Scott, Jeff Hickey, Scott Thomson, Stuart Knowles, Jeff D. Chapman, James P. Landers
Summary: This article describes a field-forward prototype instrument capable of ultra-fast thermal cycling for real-time PCR amplification of DNA and RNA. The instrument can complete 40 cycles of real-time PCR in under 10 minutes, an 84% reduction in time compared to standard assays.
Article
Multidisciplinary Sciences
Manash Jyoti Kalita, Kalpajit Dutta, Gautam Hazarika, Ridip Dutta, Simanta Kalita, Partha Pratim Das, Manash P. Sarma, Sofia Banu, Md. Ghaznavi Idris, Anjan Jyoti Talukdar, Sangitanjan Dutta, Ajanta Sharma, Subhash Medhi
Summary: As the COVID-19 pandemic continues to impact the world, the development of a cost-effective and efficient multiplex PCR detection assay for the virus has shown promising results. The study demonstrated rapid and sensitive detection of SARS-CoV-2 using innovative PCR techniques, with results comparable to commercially available kits. The novel approach has the potential for extended commercial applicability and offers a practical solution for resource-limited settings.
SCIENTIFIC REPORTS
(2021)
Article
Veterinary Sciences
Amanda E. Fine, Mathieu Pruvot, Camilla T. O. Benfield, Alexandre Caron, Giovanni Cattoli, Philippe Chardonnet, Maurizio Dioli, Thomas Dulu, Martin Gilbert, Richard Kock, Juan Lubroth, Jeffrey C. Mariner, Stephane Ostrowski, Satya Parida, Sasan Fereidouni, Enkhtuvshin Shiilegdamb, Jonathan M. Sleeman, Claudia Schulz, Jean-Jacques Soula, Yves Van der Stede, Berhe G. Tekola, Chris Walzer, Steffen Zuther, Felix Njeumi
FRONTIERS IN VETERINARY SCIENCE
(2020)
Article
Multidisciplinary Sciences
Nguyen Quynh Huong, Nguyen Thi Thanh Nga, Nguyen Van Long, Bach Duc Luu, Alice Latinne, Mathieu Pruvot, Nguyen Thanh Phuong, Le Tin Vinh Quang, Vo Van Hung, Nguyen Thi Lan, Nguyen Thi Hoa, Phan Quang Minh, Nguyen Thi Diep, Nguyen Tung, Van Dang Ky, Scott Roberton, Hoang Bich Thuy, Martin Gilbert, Leanne Wicker, Jonna A. K. Mazet, Christine Kreuder Johnson, Tracey Goldstein, Alex Tremeau-Bravard, Victoria Ontiveros, Damien O. Joly, Chris Walzer, Amanda E. Fine, Sarah H. Olson
Article
Infectious Diseases
Timothee Vergne, Mathieu Andraud, Sarah Bonnet, Nick De Regge, Marc Desquesnes, Johanna Fite, Florence Etore, Mutien-Marie Garigliany, Ferran Jori, Laetitia Lempereur, Marie-Frederique Le Potier, Elsa Quillery, Claude Saegerman, Laurence Vial, Emilie Bouhsira
Summary: Stable flies may play a minor role in the spread of ASFV at low abundances, but could be responsible for a significant proportion of transmission events at higher abundances, potentially reducing time to pig mortality. The relative contribution of stable flies is strongly influenced by factors like blood volume regurgitated and infectious dose for pigs.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Public, Environmental & Occupational Health
Chanfong Philavong, Mathieu Pruvot, Daniel Reinharz, Mayfong Mayxay, Kongsy Khammavong, Phonesavanh Milavong, Sayapeth Rattanavong, Paul F. Horwood, Philippe Dussart, Bounlom Douangngeun, Watthana Theppangna, Amanda E. Fine, Matthew T. Robinson, Paul N. Newton
ZOONOSES AND PUBLIC HEALTH
(2020)
Article
Multidisciplinary Sciences
Kirsty Officer, Mathieu Pruvot, Paul Horwood, Daniela Denk, Kris Warren, Vibol Hul, Nhim Thy, Nev Broadis, Philippe Dussart, Bethany Jackson
Review
Parasitology
Marc Desquesnes, Marisa Gonzatti, Alireza Sazmand, Sophie Thevenon, Geraldine Bossard, Alain Boulange, Geoffrey Gimonneau, Philippe Truc, Stephane Herder, Sophie Ravel, Denis Sereno, Vincent Jamonneau, Sathaporn Jittapalapong, Philippe Jacquiet, Philippe Solano, David Berthier
Summary: This review discusses the reliable and up-to-date methods for diagnosing trypanosomoses caused by trypanosomes in wild and domestic mammals. The diseases have significant impact on human and animal health, as well as livestock economy. The review covers various diagnostic methods including parasite detection, DNA detection, and antibody detection, while considering factors such as sensitivity, specificity, and cost constraints. It also addresses the challenge of diagnosing mixed infections and the importance of improving diagnostic procedures worldwide for better infection control and One Health.
PARASITES & VECTORS
(2022)
Editorial Material
Parasitology
Alireza Sazmand, Marc Desquesnes, Domenico Otranto
TRENDS IN PARASITOLOGY
(2022)
Article
Microbiology
Phirabhat Saengsawang, Serge Morand, Marc Desquesnes, Sarawut Yangtara, Tawin Inpankaew
Summary: This study detected Bartonella spp. in rodents from urban and suburban areas in Thailand, with approximately 38% of the rodents testing positive for Bartonella DNA. The Pacific rat and Asian house rat were found to harbor Bartonella spp., with four species identified in blood samples. Specific species complexes were found in particular habitats, indicating a need for a monitoring program for zoonotic Bartonella infection in high-risk areas.
Article
Agriculture, Dairy & Animal Science
Eukote Suwan, Piangjai Chalermwong, Rucksak Rucksaken, Metita Sussadee, Sarawan Kaewmongkol, Ruenruetai Udonsom, Sathaporn Jittapalapong, Bandid Mangkit
Summary: This study developed and evaluated a pETite-GRA7-based indirect ELISA method for detecting Toxoplasma gondii infection in cats. The results showed high sensitivity and specificity, with good concordance with existing diagnostic methods.
Review
Biochemistry & Molecular Biology
Denis Sereno, Bruno Oury, Anne Geiger, Andrea Vela, Ahmed Karmaoui, Marc Desquesnes
Summary: Isothermal amplification of nucleic acids has the potential to be applied for the detection of infectious agents in resource-limited areas. This review focuses on the application of this technique for Trypanosoma and Leishmania infections and proposes ways to improve its translation into real-world field applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Parasitology
Marc Desquesnes, Alireza Sazmand, Marisa Gonzatti, Alain Boulange, Geraldine Bossard, Sophie Thevenon, Geoffrey Gimonneau, Philippe Truc, Stephane Herder, Sophie Ravel, Denis Sereno, Etienne Waleckx, Vincent Jamonneau, Philippe Jacquiet, Sathaporn Jittapalapong, David Berthier, Philippe Solano, Laurent Hebert
Summary: Reliable diagnostic tools are necessary to effectively treat and control animal trypanosomoses, which can be pathogenic. This review emphasizes the implementation of previously reviewed diagnostic methods in complex scenarios, and highlights the need for an integrative approach that combines parasite detection, DNA/RNA/antigen detection, antibody detection, and epizootiological information. While antibody detection tests have high sensitivity, DNA-based methods are more specific. Further developments are required to address gaps in current diagnostic methods and to improve prevention and control of animal trypanosomoses.
PARASITES & VECTORS
(2022)
Article
Entomology
Marc Desquesnes, Kornkanok Thaisungnoen, Piangjai Chalermwong, Adele Nevot, Clement Fossaert, Antoine Lucas, Sathaporn Onju, Phoompong Boonsaen, Sathaporn Jittapalapong
Summary: This study evaluated the use of pedometers attached to the tails of feeder cattle to assess tail flick frequency and evaluate the impact of hematophagous flies. The results showed a strong correlation between fly density and both visual and pedometer records of tail flicks. The pedometers were able to reveal a high nuisance of mosquitoes that should be further investigated. In conclusion, the tail pedometers proved to be useful tools in evaluating fly density and the impact of dipterans on cattle behavior.
Article
Biochemistry & Molecular Biology
Warin Rangubpit, Eukote Suwan, Danai Sangthong, Kannika Wongpanit, Roger W. Stich, Prapasiri Pongprayoon, Sathaporn Jittapalapong
Summary: In this study, molecular dynamics simulations were conducted to investigate the binding of GTX and GSH to RmGST. It was found that GSH binds tighter and sits rigidly inside the G-site, while GTX occupies both active sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Chanchanok Duangsri, Tiina A. Salminen, Marion Alix, Sarawan Kaewmongkol, Nattaphong Akrimajirachoote, Wanthanee Khetkorn, Sathaporn Jittapalapong, Pirkko Maenpaa, Aran Incharoensakdi, Wuttinun Raksajit
Summary: Polyhydroxybutyrate (PHB) is a biocompatible and biodegradable polymer with potential applications as a replacement for fossil-derived polymers. The key enzyme for PHB production in Arthrospira platensis is PHA synthase (PhaC). This study constructed recombinant E. cloni ((R))10G cells carrying the A. platensis phaC gene (rPhaC(Ap)) and characterized the catalytic properties and structure of the overexpressed rPhaC(Ap). The obtained model of PhaC(Ap) revealed its asymmetric dimeric structure and the involvement of specific residues in substrate binding and dimerization.
Article
Parasitology
Maria Teresa Tejedor-Junco, Adrian Melian Henriquez, Pedro Pelaez Puerto, Maria Dolores Ramos, Margarita Gonzalez-Martin, Manuel Morales Doreste, Geoffrey Gimonneau, Marc Desquesnes, Sergio Martin Martel, Juan Alberto Corbera
Summary: This study investigates the occurrence of Surra, a disease caused by Trypanosoma evansi, in camels in the Canary Islands. Control measures implemented in 1997 led to a decrease in prevalence, but an outbreak in 2014 necessitated the establishment of a lazaret for isolation and further control measures. The only vector for the disease was Stomoxys calcitrans, and no other animals were found to be positive for T. evansi. The study provides evidence to aid in the control of African Animal Trypanosomosis in endemic areas and may contribute to revising control strategies for animal Trypanosomosis in camels.