Journal
VETERINARY PARASITOLOGY
Volume 184, Issue 2-4, Pages 309-316Publisher
ELSEVIER
DOI: 10.1016/j.vetpar.2011.09.021
Keywords
Babesia bovis; Cattle; Mongolia; MSA-1; MSA-2b; MSA-2c
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Funding
- Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN)
- Japanese Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science (JSPS), Japan
- Grants-in-Aid for Scientific Research [22380154, 23405041] Funding Source: KAKEN
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We conducted a molecular epidemiological study on Babesia bovis in Mongolia. Three hundred blood samples collected from cattle grazed in seven different districts were initially screened using a previously established diagnostic polymerase chain reaction (PCR) assay for the detection of B. bovis-specific DNA. Positive samples were then used to amplify and sequence the hyper-variable regions of three B. bovis genes encoding the merozoite surface antigen (MSA)-1, MSA-2b, and MSA-2c. The diagnostic PCR assay detected B. bovis among cattle populations of all districts surveyed (4.4-26.0%). Sequences of each of the three genes were highly homologous among the Mongolian isolates, and found in a single phylogenetic cluster. In particular, a separate branch was formed only by the Mongolian isolates in the MSA-2b gene-based phylogenetic tree. Our findings indicate that effective preventative and control strategies are essential to control B. bovis infection in Mongolian cattle populations, and suggest that a careful approach must be adopted when using immunization techniques. (C) 2011 Elsevier B.V. All rights reserved.
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