Journal
VETERINARY PARASITOLOGY
Volume 174, Issue 1-2, Pages 106-114Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetpar.2010.07.017
Keywords
Haemonchus contortus; Teladorsagia circumcincta; SSH; Lipase; Saposin-like protein; Transthyretin-like protein; LON-1
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Funding
- INRA Animal Health Division
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The control of gastro-intestinal nematodes remains largely based on anthelminthic treatments, however spreading of anthelmintic resistance has reduced their efficacy. The genes involved in the transition to parasitic lifestyle could constitute targets of interest to develop alternative control strategies. In the trichostrongylid nematode Haemonchus contortus, we have used a SSH (Suppressive Subtractive Hybridization) based approach to generate two distinct subtracted cDNA libraries specifically enriched in cDNA expressed during the early parasitic fourth stage larvae L4 (5 days post-infection). A total of 200 clones were subjected to dot-blot experiments and 46 clones were selected for further characterization. The 46 corresponding expressed sequence tags (EST) were found to cluster into nine contigs. The corresponding full-length cDNA was obtained for all candidates. The genes encoding potentially secreted proteins were investigated in more detail. RT-PCR experiments confirmed their specific expression or over expression from the early L4 larvae to the adult stages and search for homologs in the trichostrongylid species T. circumcincta was performed in order to investigate whether they may be novel cross-specific targets. (C) 2010 Elsevier B.V. All rights reserved.
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