Article
Parasitology
Nan Wang, Xue Bai, Jing Ding, Jiaojiao Lin, Hongfei Zhu, Xuenong Luo, Zhiqiang Fu, Chuangang Zhu, Hong Jia, Mingyuan Liu, Xiaolei Liu
Summary: The study investigated infectivity and antibody response of four Trichinella species in pigs. Results showed that larvae were detectable for T. spiralis, T. britovi, and T. pseudospiralis at 16, 17, and 16 days post-infection, respectively. Inoculation dose affected T. spiralis ML burden, and IgG antibody response was dose-dependent, with IgG1 significantly higher than IgG2a. IgM antibodies were detected earlier than IgG antibodies.
VETERINARY PARASITOLOGY
(2021)
Article
Microbiology
Michal Gondek, Sylwia Grzelak, Renata Pyz-Lukasik, Przemyslaw Knysz, Monika Ziomek, Justyna Bien-Kalinowska
Summary: This study investigated the infectivity of a Polish strain of Trichinella britovi for pigs and the antibody response of pigs to different doses of T. britovi. The results showed that T. britovi was evenly distributed in pig muscle and the highest density was found in the diaphragm pillars. Specific IgG antibodies against T. britovi were first detected 36 days post infection and reached a plateau phase from day 45 to day 62.
Article
Microbiology
Guangpu Yang, Bingqian Zhou, Kewei Chen, Zhe Hu, Wei Guo, Xiaojun Wang, Cheng Du
Summary: Novel competitive ELISA and western blot assays were developed to detect antibodies against T. equi or B. caballi. These methods have high sensitivity, no cross-reactivity with other equine diseases, and showed high coincidence rates compared to commercially available kits.
Article
Public, Environmental & Occupational Health
Maria Angeles Gomez-Morales, Giovanni Mazzarello, Elisabetta Bondi, Laura Arenare, Maria Cristina Bisso, Alessandra Ludovisi, Marco Amati, Claudio Viscoli, Elio Castagnola, Giovanni Orefice, Federica Magne, Patrizio Pezzotti, Edoardo Pozio
Summary: An outbreak of trichinellosis occurred in Genoa, Northern Italy in January-February 2015, which was traced back to a dinner served at an agritourism farm.
ZOONOSES AND PUBLIC HEALTH
(2021)
Article
Biotechnology & Applied Microbiology
Christina Seisenberger, Tobias Graf, Markus Haindl, Harald Wegele, Michael Wiedmann, Stefanie Wohlrab
Summary: Through a study comparing ELISAs and 2D-WBs for HCP monitoring, it was found that ELISAs are better suited for accurate detection of HCPs, while 2D-WBs have some limitations.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Fisheries
Evan M. Jones, Luke P. Oliver, Jie Ma, Robine H. J. Leeuwis, Veronica Myrsell, Mary R. Arkoosh, Joseph P. Dietrich, Cameron M. Schuster, Matt Hawkyard, A. Kurt Gamperl, Kenneth D. Cain
Summary: A monoclonal antibody specific to sablefish IgM was produced and demonstrated its usefulness in studying antibody-mediated immunity, vaccine development, and aquaculture efforts for this fish species. The antibody, UI-25A, can recognize membrane-bound IgM and identify immunogenic regions of A. salmonicida.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Parasitology
Eya Ben Salah, Coralie Barrera, Sana Mosbahi, Bruno Gottstein, Mar Siles-Lucas, Samia Belhassen, Abdellatif Nouri, Hamouda Babba, Laurence Millon, Wahiba Sakly
Summary: The study investigated the value of antigenic proteins from Echinococcus granulosus protoscoleces and recombinant B2t and 2B2t proteins in assessing the efficacy of surgical treatment for CE-affected children. Results showed that B2t protein could be useful for early post-surgical outcome prediction, while protoscolex proteins like P26/27 and P40 may serve as promising predictive biomarkers for post-surgical follow-up of CE cases.
PARASITES & VECTORS
(2021)
Article
Food Science & Technology
Ewa Bilska-Zajac, Weronika Korpysa-Dzirba, Aneta Belcik, Jacek Karamon, Jacek Sroka, Tomasz Cencek
Summary: Trichinellosis is a parasitic disease caused by Trichinella larvae, which can be transmitted through the consumption of undercooked meat. In Europe, the disease is often associated with the consumption of wild boar, pig, and horse meat. Illegal hunting of wild boars and lack of testing have led to an increase in trichinellosis cases in humans. This article proposes a scheme for effective epidemiological investigations on pig farms to trace the transmission mechanisms and prevent further outbreaks.
Article
Microbiology
Ewa Bilska-Zajac, Miroslaw Rozycki, Weronika Korpysa-Dzirba, Aneta Belcik, Anna Zietek-Barszcz, Magdalena Wlodarczyk-Ramus, Aneta Gontarczyk, Tomasz Cencek
Summary: Trichinella nematodes continue to circulate in various hosts, with wild boars being a primary source in Europe, but pigs could also be a cause of trichinellosis. Study in Polish pig farms showed low overall prevalence but cases of infection, highlighting the importance of constant monitoring for consumer safety.
Article
Chemistry, Analytical
Junqiu Zhang, Liwei Xu, Hongtao Jiang, Chuanlai Xu, Wenjing Liu, Ting Wu, Hua Kuang
Summary: A high-throughput immunoassay was developed for the detection of copper residues in pork tissues, utilizing an ic-ELISA method for copper ion analysis with excellent performance characteristics. The immunoassay was compared with ICP-MS for detection correlation, showing high agreement between the two methods in sample testing.
Article
Biochemical Research Methods
Qiana Mendez, Holland A. Driscoll, Gregory R. Mirando, Felicity Acca, Cassandra D. Chapados, Kezzia S. Jones, Michael Weiner, Xiaofeng Li, Mary R. Ferguson
Summary: Knowing the accuracy of an antibody's sensitivity and specificity is crucial for reliable data collection. Two validation methods, MILKSHAKE and Sundae, are described for antibodies binding post-translationally modified proteins. These methods improve evaluation of commercially available antibodies and identify off-target effects for research and therapeutic purposes.
JOURNAL OF IMMUNOLOGICAL METHODS
(2023)
Article
Biochemistry & Molecular Biology
Muhammad Faheem, Arif-ullah Khan, Muhammad Waqas Saleem, Fawad Ali Shah, Fawad Ali, Abdul Waheed Khan, Shupeng Li
Summary: This study explored the effects of natural compounds, berbamine, bergapten, and carveol, on neuroinflammatory pain associated with paclitaxel. The results showed that these compounds attenuated thermal hypersensitivity, improved motor function, normalized body weight changes, and increased the pain threshold. They also demonstrated neuroprotective effects by regulating molecular pathways.
Article
Parasitology
Vladislav A. Lobanov, Kelly A. Konecsni, Randy W. Purves, W. Brad Scandrett
Summary: This study developed a recombinant protein of Trichinella spiralis serpin using recombinant technology and applied it in indirect ELISA for detecting exposure to Trichinella in pigs. The Serpin ELISA showed high specificity and sensitivity in commercial pig herds, but lower sensitivity for detecting antibodies induced by experimental infections of pigs with sylvatic Trichinella species.
VETERINARY PARASITOLOGY
(2022)
Article
Microbiology
Sarfraz-ur-Rahman, Haroon Akbar, Muhammad Zubair Shabbir, Ubaid Ullah, Muhammad Imran Rashid
Summary: The study evaluated the diagnostic value of the recombinant SAG1 antigen for T. gondii-IgG screening. The ELISA method showed high sensitivity and specificity, but may produce false positive results in some cases.
Article
Parasitology
Marie-Kristin Raulf, Daniela Jordan, Herbert Auer, Jens M. Warnecke, Bernd Lepenies, Christina Strube
Summary: This study evaluated the newly developed Anti-Toxocara-ELISA and Anti-Toxocara-Westernblot prototypes, which showed high sensitivity and specificity, indicating high reliability of these newly developed tests.
Article
Biotechnology & Applied Microbiology
M. Corro, S. Saleh-Mohamed-Lamin, S. Jatri-Hamdi, B. Slut-Ahmed, S. Mohamed-Lejlifa, S. Di Lello, D. Rossi, A. Broglia, L. Vivas-Alegre
JOURNAL OF FOOD PROTECTION
(2012)
Article
Parasitology
Jochen Reetz, Karsten Noeckler, Sabine Reckinger, Maria Margarida Vargas, Wolf Weiske, Alessandro Broglia
PARASITOLOGY INTERNATIONAL
(2009)
Article
Parasitology
A. Broglia, T. Weitzel, G. Harms, S. M. Caccio, K. Noeckler
PARASITOLOGY RESEARCH
(2013)
Article
Parasitology
A. Broglia, C. Kapel
VETERINARY PARASITOLOGY
(2011)
Article
Parasitology
A. Mayer-Scholl, A. Broglia, S. Reckinger, K. Noeckler
VETERINARY PARASITOLOGY
(2014)
Article
Veterinary Sciences
N. Pantchev, A. Broglia, B. Paoletti, M. Globokar Vrhovec, A. Bertram, K. Noeckler, S. M. Caccio
Review
Virology
Alexander J. McMenamin, Katie F. Daughenbaugh, Fenali Parekh, Marie C. Pizzorno, Michelle L. Flenniken
Article
Biodiversity Conservation
Gabriele Volpato, Antonello Di Nardo, Davide Rossi, Saleh M. Lamin Saleh, Alessandro Broglia
JOURNAL OF ETHNOBIOLOGY AND ETHNOMEDICINE
(2013)
Article
Veterinary Sciences
Eyal Klement, Alessandro Broglia, Sotiria-Eleni Antoniou, Vangelis Tsiamadis, E. Plevraki, Tamas Petrovic, Vladimir Polacek, Zoran Debeljak, Aleksandra Miteva, Tsviatko Alexandrov, Drago Marojevic, Ledi Pite, Vanja Kondratenko, Zoran Atanasov, Simon Gubbins, Arjan Stegeman, Jose Cortinas Abrahantes
PREVENTIVE VETERINARY MEDICINE
(2020)
Article
Veterinary Sciences
Simon Gubbins, Arjan Stegeman, Eyal Klement, Ledi Pite, Alessandro Broglia, Jose Cortinas Abrahantes
PREVENTIVE VETERINARY MEDICINE
(2020)
Article
Veterinary Sciences
E. S. M. Tuppurainen, S-E Antoniou, E. Tsiamadis, M. Topkaridou, T. Labus, Z. Debeljak, B. Plavsic, A. Miteva, T. Alexandrov, L. Pite, J. Boci, D. Marojevic, V Kondratenko, Z. Atanasov, B. Murati, Z. Acinger-Rogic, L. Kohnle, P. Calistri, A. Broglia
PREVENTIVE VETERINARY MEDICINE
(2020)
Article
Anesthesiology
E. Li
Summary: Surgical patients with peri-operative or recent SARS-CoV-2 infection are at increased risk of postoperative venous thromboembolism compared to those without a history of SARS-CoV-2. However, the optimal prophylaxis and treatment for venous thromboembolism in this patient population remains unknown. Venous thromboembolism is independently associated with 30-day mortality.
Article
Food Science & Technology
John Berezowski, Katinka De Balogh, Fernanda C. Dorea, Simon Ruegg, Alessandro Broglia, Gabriele Zancanaro, Andrea Gervelmeyer
Summary: This report provides guidance for Member States planning to apply for the direct grants to Member States' authorities under the work programme 'CP-g-22-04.01'. The report identifies the priority pathogens for coordinated surveillance, including Crimean Congo haemorrhagic fever, echinococcosis, hepatitis E, highly pathogenic avian influenza (HPAI), influenza in swine, Lyme disease, Q-fever, Rift Valley fever, tick-borne encephalitis, West Nile fever, and Disease X (Disease Y of animals). It also suggests surveillance activities for these pathogens and encourages cross-sectoral collaborations.
Article
Food Science & Technology
Cornelia Adlhoch, Alice Fusaro, Jose L. Gonzales, Thijs Kuiken, Angeliki Melidou, Grazina Mirinaviciute, Eric Niqueux, Karl Stahl, Christoph Staubach, Calogero Terregino, Francesca Baldinelli, Alessandro Broglia, Lisa Kohnle
Summary: Between 29 April and 23 June 2023, outbreaks of highly pathogenic avian influenza (HPAI) A(H5N1) virus (clade 2.3.4.4b) were reported in domestic and wild birds across 25 European countries. The mulard ducks for foie gras production in Southwest France were heavily affected, while the overall A(H5N1) situation in poultry in Europe and worldwide has improved. The virus also affected wild birds, especially black-headed gulls and other seabird species, and showed increased mortality in adults and juveniles. In addition, cases of A(H5N1) virus infection were found in domestic cats and a caracal in Poland.
Article
Food Science & Technology
Jane Richardson, Caryl Lockhart, Stefano Pongolini, William B. Karesh, Matthew Baylis, Tony Goldberg, Jan Slingenbergh, Paul Gale, Tommaso Venturini, Mike Catchpole, Katinka de Balogh, Marco Pautasso, Alessandro Broglia, Franck Berthe, Jan Schans, Guy Poppy