Article
Biochemistry & Molecular Biology
Harpreet Kaur, Vinay Modgil, Naveen Chaudhary, Balvinder Mohan, Neelam Taneja
Summary: Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) and their extended-spectrum beta-lactamase (ESBL) production pose a significant threat to current antimicrobial treatment options. To overcome this challenge, the identification of new therapeutic targets is crucial. In this study, we used computational tools to identify potential drug targets against the ESBL-producing UPEC strain NA114, focusing on chokepoint, virulence, and resistance genes. Through a hierarchical in silico technique, we discovered seven distinct targets for this pathogen that showed no similarity to the human proteome. These targets, involved in bacterial peptidoglycan biosynthesis and inositol phosphate metabolism, hold promise for the design of effective drugs against MDR UPEC.
Article
Agriculture, Dairy & Animal Science
Zui Wang, Qin Lu, Xiaohui Mao, Li Li, Junfeng Dou, Qigai He, Huabin Shao, Qingping Luo
Summary: ESBL-producing E.coli strains isolated from chickens in central China showed high resistance to cephalosporins and harbored diverse ESBL-encoding genes. These strains pose a significant public health risk.
Article
Microbiology
Mahesh Kumar Chaudhary, Indrani Jadhav, Megha Raj Banjara
Summary: This study aimed to identify ESBL-producing genes from Escherichia coli isolates in a referral-level tertiary care hospital in Lalitpur. The study found a high prevalence of multidrug-resistant E. coli isolates with high resistance rates to commonly used antibiotics, as well as an increased predominance of the major gene type bla(TEM). This poses a serious threat to clinicians and microbiologists.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Infectious Diseases
Daniel Martak, Julia Guther, Tess D. Verschuuren, Benoit Valot, Nadine Conzelmann, Stefanie Bunk, M. Eugenia Riccio, Elena Salamanca, Alexandre Meunier, Charles P. Henriot, Caroline Pressacco Brossier, Xavier Bertrand, Ben S. Cooper, Stephan Harbarth, Evelina Tacconelli, Ad C. Fluit, Jesus Rodriguez-Bano, Jan A. J. W. Kluytmans, Silke Peter, Didier Hocquet
Summary: This study in five European cities found that food items were not the main source of ESBL-Ec and ESBL-Kp, as the strains from contaminated environments were genetically distinct from those in food. The findings suggest that human-to-human transmission is possibly the most frequent route of ESBL-Ec and ESBL-Kp transmission in high-income countries.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Jerome Ambroise, Elmostafa Benaissa, Leonid Mwana Wa Bene Irenge, El Mehdi Belouad, Bertrand Bearzatto, Jean-Francois Durant, Jamal Badir, Mostafa Elouennass, Jean-Luc Gala
Summary: This study aimed to provide data on ESBL-producing E. coli isolates from patients with extraintestinal infections in Morocco. The results underscore the pre-eminence of ST131 as the major factor driving the expansion of ExPEC in the Rabat region.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Review
Microbiology
Shweta R. Singh, Alvin Kuo Jing Teo, Kiesha Prem, Rick Twee-Hee Ong, Elizabeth A. Ashley, H. Rogier van Doorn, Direk Limmathurotsakul, Paul Turner, Li Yang Hsu
Summary: Despite limited knowledge on the prevalence of ESBL-E and CPE in the Greater Mekong Subregion, recent trends show an increase in ESBL-producing E. coli in clinical and carriage specimens. Risk factors associated with ESBL-E isolation include recent antibiotic exposure, chronic kidney disease, and other co-morbidities. Collaboration on standardizing study methods is needed to improve monitoring and surveillance in the region, especially in community settings of Laos, Myanmar, Cambodia, and Yunnan and Guangxi provinces of China.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Maya Frank Wolf, Raneen Abu Shqara, Karina Naskovica, Inna Amdur Zilberfarb, Inshirah Sgayer, Daniel Glikman, Hagai Rechnitzer, Vered Fleisher Sheffer, Jacob Bornstein
Summary: Maternal carriage and vertical transmission of ESBL-E pose a threat to preterm infants, leading to higher rates of complications. In this study, a correlation was found between placental inflammation and maternal ESBL-E colonization, highlighting the importance of surveillance and preventive measures.
Article
Medicine, General & Internal
Monica Romero Nieto, Sara Maestre Verdu, Vicente Gil, Carlos Perez Barba, Jose Antonio Quesada Rico, Reyes Pascual Perez
Summary: This study identified male sex, smoking, hypertension, urinary incontinence, and recurrent urinary tract infections as independent factors associated with the presence of ESBL-producing E. coli in patients with acute community-acquired pyelonephritis. The multivariate logistic models could correctly classify individuals with ESBL-producing E. coli infection in 80.2% of cases.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Microbiology
Carine Laurence Yehouenou, Bert Bogaerts, Sigrid C. J. De Keersmaecker, Nancy H. C. Roosens, Kathleen Marchal, Edmond Tchiakpe, Dissou Affolabi, Anne Simon, Francis Moise Dossou, Kevin Vanneste, Olivia Dalleur
Summary: The study utilized whole-genome sequencing to investigate antimicrobial resistance and phylogenomic relationships among 19 ESBL-positive E.coli isolates collected from surgical site infections in Benin in 2019. The findings revealed diverse sequence types and the presence of various resistance genes, highlighting the potential of WGS as an effective early warning system for emerging pathogens and antimicrobial resistance.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Nina Langkabel, Janine Burgard, Sabrina Freter, Reinhard Fries, Diana Meemken, Luppo Ellerbroek
Summary: ESBL-producing E. coli is considered a major hazard in poultry, with poultry meat being a potential source of human infection. This study found ESBL-producing E. coli in broiler breast skin samples from different abattoirs, with the lowest incidence in the abattoir using conventional immersion scalding. Good kitchen hygiene is important to prevent infection by ESBL-producing E. coli and highly pathogenic phylogroups through chicken meat.
Article
Microbiology
Wen-Chi Chen, Chih-Hsin Hung, Yao-Shen Chen, Jin-Shiung Cheng, Susan Shin-Jung Lee, Fan-Chen Tseng, Ming-Fang Cheng, Jiun-Ling Wang
Summary: This study found a higher frequency of ST 131 strains and longer hospital stay in patients with cirrhosis in the ESBLEC group compared to the NESBLEC group in bloodstream infections. The 30-day re-admission/mortality rates were similar between the two groups, but ST 131 strains were associated with 30-day re-admission.
Review
Public, Environmental & Occupational Health
H. Namikawa, W. Imoto, K. Yamada, Y. Tochino, Y. Kaneko, H. Kakeya, T. Shuto
Summary: This review evaluated 10 observational studies and found that previous antibiotic therapy, especially with cephalosporins and quinolones, as well as urinary catheter use, were predictive factors for ESBL-producing E. coli-induced bacteraemia.
JOURNAL OF HOSPITAL INFECTION
(2023)
Article
Infectious Diseases
Jolinda de Korne-Elenbaas, Boas C. L. van der Putten, Niels D. M. Boek, Amy Matser, Constance Schultsz, Sylvia M. Bruisten, Alje P. van Dam
Summary: This study investigates the prevalence and transmission of ESBL-Ec strains among MSM in Amsterdam. It identifies unique ESBL-Ec strains involved in putative transmission between participants, suggesting a high probability of ESBL-Ec transmission in this population.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Agriculture, Dairy & Animal Science
Kwang-Won Seo
Summary: A simple and fast method for detecting ESBL- and pAmpC beta-lactamase-producing bacteria in samples with high bacterial load is important for clinical antimicrobial selection. The disk diffusion test, DDST, and multiplex PCR were used for phenotypic and genotypic detection of ESBL and pAmpC. The majority of isolates carried beta-lactamase genes, especially in samples grown on MacConkey agar containing cephalothin or ceftiofur.
Article
Infectious Diseases
Nguyen Thanh Viet, Vu Van Du, Nghiem Duc Thuan, Hoang Van Tong, Nguyen Linh Toan, Can Van Mao, Nguyen Van Tuan, Srinivas Reddy Pallerla, Dennis Nurjadi, Thirumalaisamy P. Velavan, Ho Anh Son
Summary: The study in Vietnam found that 30% of pregnant women had colonization of E. coli and Klebsiella species in their birth canals, with 47% of them producing ESBL. Carapenem resistance was low overall, but over 20% of Klebsiella species were resistant. This highlights the need for surveillance and molecular typing to combat the high rate of ESBL-producers and carbapenem resistance in Enterobacterales in Vietnam.
Editorial Material
Veterinary Sciences
I. C. Johns
EQUINE VETERINARY EDUCATION
(2019)
Article
Polymer Science
Nor Fadhilah Kamaruzzaman, Maria de Fatima Pina, Alexandru Chivu, Liam Good
Article
Veterinary Sciences
D. Pollard, C. E. Wylie, J. R. Newton, K. L. P. Verheyen
EQUINE VETERINARY JOURNAL
(2019)
Article
Reproductive Biology
A. M. de Mestre, B. V. Rose, Y. M. Chang, D. C. Wathes, K. L. P. Verheyen
Article
Veterinary Sciences
D. Pollard, C. E. Wylie, K. L. P. Verheyen, J. R. Newton
BMC VETERINARY RESEARCH
(2019)
Article
Biotechnology & Applied Microbiology
Chinwe U. Chukwudi, Liam Good
JOURNAL OF ANTIBIOTICS
(2019)
Article
Veterinary Sciences
Rekaz A. Ibrahim, Tillie L. Cryer, Shawkat Q. Lafi, Ehab-Abu Basha, Liam Good, Yaser H. Tarazi
BMC VETERINARY RESEARCH
(2019)
Article
Polymer Science
Anna Worsley, Kristin Vassileva, Janice Tsui, Wenhui Song, Liam Good
Article
Multidisciplinary Sciences
Chinwe Uzoma Chukwudi, Liam Good
Review
Public, Environmental & Occupational Health
Bugwesa Z. Katale, Gerald Misinzo, Stephen E. Mshana, Harriet Chiyangi, Susana Campino, Taane G. Clark, Liam Good, Mark M. Rweyemamu, Mecky Matee
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2020)
Article
Pharmacology & Pharmacy
Alexandru Chivu, Kantaraja Chindera, Graca Mendes, Angela An, Brian Davidson, Liam Good, Wenhui Song
Summary: This study demonstrates the efficient intracellular delivery of pDNA and ODNs using PHMB as a nano-vector, with comparable or even higher gene expression levels than PEI analogues in various cell lines. The well-established safety profile of PHMB makes it a promising candidate for further research and potential clinical applications.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Review
Biotechnology & Applied Microbiology
Winnie Ntow-Boahene, David Cook, Liam Good
Summary: Advancements in medicine leading to rising global populations have increased the number of patients susceptible to fungal infections, impacting plant pathogens on preharvested crops and stored food. Current antifungal strategies face limitations, leading to the exploration of antimicrobial polymers as an alternative strategy that can be combined with other antimicrobial compounds for synergistic effects. Antifungal polymers and nanocomposites show promising antifungal effects and reduced toxicity compared to traditional small molecule antifungal drugs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Oluwawemimo Adebowale, Liam Good
BIOLOGY METHODS & PROTOCOLS
(2020)
Article
Veterinary Sciences
Pamela Martinez-Orellana, Marta Baxarias, Liam Good, Laia Solano-Gallego
VETERINARY SCIENCES
(2020)
Article
Multidisciplinary Sciences
Oluwawemimo O. Adebowale, Shan Goh, Liam Good