Article
Infectious Diseases
Joaquim Vines, Norma Fabregas, Daniel Perez, Anna Cusco, Rocio Fonticoba, Olga Francino, Lluis Ferrer, Lourdes Migura-Garcia
Summary: In this study, the correlation between phenotype and genotype regarding antimicrobial resistance of Staphylococcus pseudintermedius from healthy dogs was assessed using Nanopore sequencing technology. Good correlation was observed for some antimicrobials, while correlation was low for others. Multiple antibiotic resistance genes were also found to be located on plasmids. This study provides important insights into the antimicrobial resistance of S. pseudintermedius from healthy dogs.
Article
Microbiology
Vanessa Silva, Ana Oliveira, Vera Manageiro, Manuela Canica, Diogo Contente, Rosa Capita, Carlos Alonso-Calleja, Isabel Carvalho, Jose L. Capelo, Gilberto Igrejas, Patricia Poeta
Summary: The study aimed to characterize the antimicrobial resistance and clonal lineages of MRSP isolated from canine cutaneous pyoderma. The majority of isolates belonged to ST123 and exhibited multidrug resistance, posing a significant threat to animal health.
Article
Infectious Diseases
Kirill V. Ovchinnikov, Christian Kranjec, Tage Thorstensen, Harald Carlsen, Dzung B. Diep
Summary: The rise of antibiotic-resistant bacteria is a major challenge in human and veterinary medicine. This study highlights the importance of searching for antimicrobials for veterinary applications to prevent the misuse of frontline clinical antibiotics. The research shows that a combination of bacteriocins, namely micrococcin P1 and EntEJ97s, with conventional antibiotic penicillin G can synergistically combat methicillin-resistant Staphylococcus pseudintermedius (MRSP), a common pathogen in animal skin infections, and prevent the development of resistance. It also demonstrates the therapeutic potential of this combination in a murine skin infection model.
Article
Infectious Diseases
Elisa Rampacci, Tommaso Felicetti, Giada Cernicchi, Valentina Stefanetti, Stefano Sabatini, Fabrizio Passamonti
Summary: One approach to treating antibiotic-resistant bacteria is to co-administer efflux pump inhibitors (EPIs) with antibiotics to break resistances connected with antibacterial efflux. In this study, ten compounds were evaluated for their ability to inhibit efflux and synergize with different antibiotics in Staphylococcus pseudintermedius. Hit compounds 1, 6, and 8 were considered the best EPIs for S. pseudintermedius. These findings provide valuable data for further optimization and development of EPIs for treating staphylococcal infections.
Article
Medicine, General & Internal
Sang Guen Kim, Sib Sankar Giri, Saekil Yun, Sang Wha Kim, Se Jin Han, Jun Kwon, Woo Teak Oh, Sung Bin Lee, Yong Ho Park, Se Chang Park
Summary: This study characterized antimicrobial resistance in Staphylococcus pseudintermedius from canine samples, and isolated two novel bacteriophages, pSp-J and pSp-S, from canine pet parks in South Korea with potential to control methicillin-resistant S. pseudintermedius. The phages showed stability and efficacy in preventing and degrading biofilms at different doses, demonstrating their potential for treating this pathogen.
FRONTIERS IN MEDICINE
(2021)
Article
Chemistry, Medicinal
Pavarish Jantorn, Hawaree Heemmamad, Tanawan Soimala, Saowakon Indoung, Jongkon Saising, Julalak Chokpaisarn, Warapond Wanna, Varomyalin Tipmanee, Dennapa Saeloh
Summary: Staphylococcus pseudintermedius is a zoonotic pathogen that can cause life-threatening infections in animals and humans. The study focused on methicillin-resistant S. pseudintermedius and its ability to produce biofilms, with results showing a 28.30% MRSP prevalence among isolates in dogs at Prince of Songkla University. The study observed co-resistant patterns in methicillin-resistant strains against various antibiotics.
Article
Infectious Diseases
Andrea T. Fessler, Anissa D. Scholtzek, Angela R. Schug, Barbara Kohn, Christiane Weingart, Anne-Kathrin Schink, Astrid Bethe, Antina Luebke-Becker, Stefan Schwarz
Summary: A study of 114 Staphylococcus isolates from companion animals revealed variations in susceptibility to antimicrobial agents, with a proportion of isolates carrying resistance genes. However, no signs of biocide resistance were detected at present.
Article
Infectious Diseases
Alessandro Bellato, Patrizia Robino, Maria Cristina Stella, Laura Scarrone, Daniela Scalas, Patrizia Nebbia
Summary: This study aimed to assess the resistance of Staphylococcus pseudintermedius isolates, against a broad panel of antibacterials, including exclusive to human medicine. The results showed that these strains had the highest resistance against veterinary medicine antibacterials, but there was also resistance against antibacterials exclusive to human medicine.
Article
Biology
Usasom Khongsri, Peerawit Chongrattanameteekul, Sineenart Chantarachart, Kornravee Photichai, Nittaya Chanayat, Thanya Varinrak, Raktham Mektrirat, Nuttawan Srifawattana
Summary: The prevalence of MRSP causing pyoderma is changing gradually, with annual variations. Research on cotrimazole susceptibility to MRSP is limited, but the empirical use of cotrimazole remains interesting. This study aimed to evaluate the susceptibility of cotrimazole to canine pyoderma MRSP isolates.
Article
Biochemistry & Molecular Biology
Mariela E. Srednik, Claudia A. Perea, Gabriela I. Giacoboni, Jessica A. Hicks, Christine L. Foxx, Beth Harris, Linda K. Schlater
Summary: This study comparatively analyzed the population structure of Staphylococcus pseudintermedius associated with canine pyoderma in Argentina and the United States. The findings revealed that up to 62% of isolates exhibited multi-drug resistance. Several resistance genes and mutations were identified, indicating an emerging problem in antimicrobial resistance. Additionally, commonalities, such as the presence of sequence type ST71 in isolates from both countries, were observed. This study is important for monitoring the evolution and dissemination of antimicrobial resistance and gaining further insights into the development of this pathogen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jareerat Aiemsaard, Chuchat Kamollerd, Supawadee Seubsasana, Eakachai Thongkham, Pairoj Vonghataipaisarn
Summary: The study suggests that lemongrass essential oil in combination with cephalexin has the potential to treat superficial pyoderma in dogs caused by Staphylococcus pseudintermedius. Lemongrass essential oil showed a partial synergistic effect with cephalexin, with its antimicrobial effects being concentration-dependent and time-dependent.
Article
Parasitology
Gabriela Merker Breyer, Bianca Fagundes Saggin, Silvia de Carli, Maria Eduarda Rocha Jacques da Silva, Mateus Matiuzzi da Costa, Bertram Brenig, Vasco Ariston de Carvalho Azevedo, Marisa Ribeiro de Itapema Cardoso, Franciele Maboni Siqueira
Summary: This study aims to uncover the virulence profile of Staphylococcus pseudintermedius strains from canine origin. MRSP and MSSP strains were compared to determine their clinical implications. The findings highlight the wide spread of highly virulent and drug-resistant strains, posing potential concerns for One Health issues.
Article
Veterinary Sciences
Zhihao Wang, Long Guo, Jun Li, Jianji Li, Luying Cui, Junsheng Dong, Xia Meng, Chen Qian, Heng Wang
Summary: This study investigated the distribution rate, resistance to antibiotics, biofilm-producing ability, and dissemination of virulence factors in strains of Staphylococcus pseudintermedius isolated from healthy dogs and dogs with keratitis in Yangzhou, China. The results showed that S. pseudintermedius was the most common bacterial isolate in both groups, with a prevalence of 20.9% in the healthy group and 23.08% in the keratitis group. The majority of S. pseudintermedius isolates were susceptible to rifampin, oxacillin, and neomycin, but resistant to tetracycline. Most isolates exhibited virulence factors and were classified as biofilm producers.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Veterinary Sciences
Stephanie A. Lynch, Karla J. Helbig
Summary: Staphylococcus pseudintermedius is a significant pathogenic bacterium in veterinary medicine, causing various infections in canines. The high prevalence of methicillin-resistant strains is concerning, prompting research into novel treatment avenues. Multiple new approaches, including vaccines and phage therapy, show promise in combatting MRSP infections in dogs. Further research is needed to address the challenges associated with MRSP.
VETERINARY SCIENCES
(2021)
Article
Multidisciplinary Sciences
K. Bierowiec, M. Miszczak, A. Korzeniowska-Kowal, A. Wzorek, D. Plokarz, A. Gamian
Summary: This study investigated the occurrence of Staphylococcus pseudintermedius in cats, revealing a prevalence of 2.49% in healthy cats and 7.61% in sick cats. Cats living with dogs were more likely to be colonized with the bacterium. Multidrug resistance was a common phenomenon among the isolates, highlighting the importance of monitoring antibiotic resistance in cats.
SCIENTIFIC REPORTS
(2021)
Article
Public, Environmental & Occupational Health
Camillo Martino, Elisa Rampacci, Ilaria Pierini, Monica Giammarioli, Valentina Stefanetti, Doreene R. Hyatt, Andrea Ianni, Giovanni Di Paolos, Mauro Coletti, Fabrizio Passamonti
Summary: Researchers investigated blood and liver samples from domestic pigs in the Abruzzo region of Italy and found a high prevalence of anti-HEV antibodies in swine serum, but no HEV RNA in liver samples. This suggests that while local pigs in Abruzzo are circulating HEV, the hypothesis of foodborne zoonotic transmission may not fully explain the high HEV prevalence in the region. Further research on wild fauna, carrier pigs, and the meat supply chain is needed to understand sources of human infection and occupational risks.
EUROPEAN JOURNAL OF PUBLIC HEALTH
(2021)
Article
Biotechnology & Applied Microbiology
M. L. Marenzoni, M. B. Conti, E. Rossi, R. Rinoldo, R. Franceschini, E. Tesei, F. Porciello, S. Mancini, B. Favi, M. Gamboni, P. Boni, O. Raffaele, C. Desario, M. P. Franciosini, C. Buonavoglia, N. Decaro
Summary: The study developed a protocol for environmental sampling to detect parvoviruses of dogs and cats. Molecular assays were the most sensitive methods, able to identify even very low amounts of viral DNA. The protocol is important for identifying possible sources of infection and evaluating the efficacy of disinfection protocols in the environment.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Infectious Diseases
Elisa Rampacci, Maria Luisa Marenzoni, Rolando Cannalire, Donatella Pietrella, Stefano Sabatini, Stefano Giovagnoli, Tommaso Felicetti, Marco Pepe, Fabrizio Passamonti
Summary: This study created a new strain (Rhodococcus equi(EtBr25)) by exposing R. equi ATCC 33701 to ethidium bromide (EtBr), which increased efflux and resistance to azithromycin and ciprofloxacin. Gene expression analysis showed that the overexpression of a Major Facilitator Superfamily transporter may contribute to this resistance. The findings suggest that clinically important isolates may have a low-level resistance that could be overcome by EPI-based therapies.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Agriculture, Dairy & Animal Science
Lorenzo Pisello, Claudio Forte, Nicoletta D'Avino, Rocco Pisano, Doreene Rose Hyatt, Fabrizio Rueca, Fabrizio Passamonti
Summary: This study found that there are differences in IgG content in colostrum between beef and dairy cows, with beef cows having higher IgG concentrations. The digital Brix refractometer showed good sensitivity and specificity in detecting IgG concentration, but a breed-based cut-point is recommended for accuracy.
JOURNAL OF DAIRY RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Maria Teresa Antognoni, Maria Luisa Marenzoni, Ambra Lisa Misia, Luca Avellini, Elisabetta Chiaradia, Alessandra Gavazza, Arianna Miglio
Summary: The study found that pre-storage leukoreduction can partially protect against storage lesions in blood units, emphasizing its importance for stored blood.
Article
Microbiology
Elisa Rampacci, Michele Trotta, Caterina Fani, Serenella Silvestri, Valentina Stefanetti, Chiara Brachelente, Antonella Mencacci, Fabrizio Passamonti
Summary: Staphylococcus pseudintermedius is the primary cause of canine cutaneous infections and is sporadically isolated from humans. Rapidly emerging antibiotic-resistant strains are creating serious health concerns, highlighting the importance of accurate and timely antimicrobial susceptibility testing for patient care.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
Laura Musa, Patrizia Casagrande Proietti, Maria Luisa Marenzoni, Valentina Stefanetti, Tana Shtylla Kika, Francesca Blasi, Chiara Francesca Magistrali, Valeria Toppi, David Ranucci, Raffaella Branciari, Maria Pia Franciosini
Summary: The study compared antibiotic susceptibility of Escherichia coli from organic, antibiotic-free, and conventional broiler farms, showing higher prevalence of resistant strains in organic systems for certain antibiotics but lower prevalence for cephalosporins, representing a protective factor. Monitoring antibiotic resistance in the production chain can help detect inherent risks in different rearing systems.
Article
Virology
Chiara Arcangeli, Martina Torricelli, Carla Sebastiani, Daniele Lucarelli, Marcella Ciullo, Fabrizio Passamonti, Monica Giammarioli, Massimo Biagetti
Summary: The study genetically characterized SRLVs circulating in central Italy, revealing the presence of genotypes A and B in the samples, with no recombination events detected. Mutations were mainly localized in the VR-2 region, and the dN/dS ratio indicated the existence of purifying selection.
Review
Veterinary Sciences
Maria Luisa Marenzoni, Chiara De Waure, Peter J. Timoney
Summary: This study aimed to evaluate the efficacy of commercial or candidate vaccines against EHV-1 through a systematic review and meta-analysis of randomized controlled trials. The results showed that vaccination generally improved the clinical and virological outcomes of EHV-1 infection, although the effects were minimal. The limitations of the study included heterogeneity and poor reporting quality.
EQUINE VETERINARY JOURNAL
(2023)
Article
Virology
Katia Cappelli, Chiara Grazia De Ciucis, Samanta Mecocci, Tiziana Nervo, Maria Ines Crescio, Marco Pepe, Rodolfo Gialletti, Daniele Pietrucci, Laura Federica Migone, Silvia Turco, Luca Mechelli, Fabrizio Passamonti, Carlo Drago, Gian Guido Donato, Katia Varello, Paola Modesto, Giovanni Chillemi, Alessandro Ghelardi, Elisabetta Razzuoli
Summary: This study aimed to estimate the genoprevalence of Equine Papillomavirus 2 (EcPV2) in clinically healthy horses in Italy and evaluate their innate immune response. The results showed that approximately 30.3% of the healthy horse samples tested positive for EcPV2-L1 DNA and 48% of the positive samples expressed the L1 gene. There were no statistically significant differences in genoprevalence based on sex, age, and origin, but Thoroughbred horses had the highest risk of infection. In mares specifically, the study found a higher positivity rate in pluriparous and mares subjected to natural reproduction. Samples expressing the L1 gene showed an increased expression of IL1B and IL12p40, and a decreased expression of RANKL and TGFB, indicating an effective immune response.
Article
Veterinary Sciences
Maria Luisa Marenzoni, Alessandro Troisi, Ilaria Gallo, Lakamy Sylla, Nicola Decaro
Summary: The Canid alphaherpesvirus 1 infection can have varied outcomes in puppies and viral spread may be underestimated within the same litter. Early detection and effective control measures are crucial in preventing the spread of infection.
VETERINARY RECORD CASE REPORTS
(2022)
Article
Infectious Diseases
Elisa Rampacci, Tommaso Felicetti, Donatella Pietrella, Stefano Sabatini, Fabrizio Passamonti
Summary: This study performed in silico prediction of drug efflux pumps in Staphylococcus pseudintermedius and investigated their role in resistance to antibiotics and biocidal agents, as well as biofilm formation. The results showed that EPs, particularly NorA, play a complex role in resistance development and are associated with increased biofilm production. However, the regulatory mechanisms of EPs in S. pseudintermedius remain to be explored.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Veterinary Sciences
Giulia Maggi, Mattia Tessadori, Maria Luisa Marenzoni, Francesco Porciello, Domenico Caivano, Maria Chiara Marchesi
Summary: Esophageal and gastric foreign bodies commonly occur in small animal practices. This study reviewed the medical records of 92 animals undergoing endoscopic removal of esophageal and gastric foreign bodies to evaluate the factors that can influence the success rate and timing of the procedure. The study found that the success rate and timing of the endoscopic removal can be influenced by factors such as the size and age of the animals, the localization of the foreign bodies, the device used, and the operator's experience.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Domenico Caivano, Maria Chiara Marchesi, Piero Boni, Fabrizio Passamonti, Noemi Venanzi, Elvio Lepri
Summary: Bacterial endocarditis is a common cardiac disease in adult cattle, often associated with congenital heart defects like a ventricular septal defect. The case study presented symptoms of dysorexia and sudden decrease in milk production, with clinical examination revealing irregular heart rate and atrial fibrillation. Echocardiography detected a large vegetation on the endocardium, highlighting the importance of early diagnosis and treatment for such conditions.
VETERINARY SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Lorenzo Pisello, Giulia Sala, Fabrizio Rueca, Fabrizio Passamonti, Davide Pravettoni, Saverio Ranciati, Antonio Boccardo, Domenico Bergero, Claudio Forte
Summary: This study aimed to identify the associations between calf-level management practices and farm characteristics with disease incidence, mortality, and serum IgG concentration in Chianina beef-suckler calves. Results showed that prepartum vaccination and bottle-fed colostrum were associated with serum IgG concentration, while birth season and serum IgG concentration were linked to neonatal calf diarrhea. Family-owned farms were identified as an important factor related to respiratory disease episodes.
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2021)
