Article
Infectious Diseases
Kirill V. Ovchinnikov, Christian Kranjec, Tage Thorstensen, Harald Carlsen, Dzung B. Diep
Summary: The rise of antibiotic-resistant bacteria is a major challenge in human and veterinary medicine. This study highlights the importance of searching for antimicrobials for veterinary applications to prevent the misuse of frontline clinical antibiotics. The research shows that a combination of bacteriocins, namely micrococcin P1 and EntEJ97s, with conventional antibiotic penicillin G can synergistically combat methicillin-resistant Staphylococcus pseudintermedius (MRSP), a common pathogen in animal skin infections, and prevent the development of resistance. It also demonstrates the therapeutic potential of this combination in a murine skin infection model.
Article
Parasitology
Gabriela Merker Breyer, Bianca Fagundes Saggin, Silvia de Carli, Maria Eduarda Rocha Jacques da Silva, Mateus Matiuzzi da Costa, Bertram Brenig, Vasco Ariston de Carvalho Azevedo, Marisa Ribeiro de Itapema Cardoso, Franciele Maboni Siqueira
Summary: This study aims to uncover the virulence profile of Staphylococcus pseudintermedius strains from canine origin. MRSP and MSSP strains were compared to determine their clinical implications. The findings highlight the wide spread of highly virulent and drug-resistant strains, posing potential concerns for One Health issues.
Article
Microbiology
Vanessa Silva, Ana Oliveira, Vera Manageiro, Manuela Canica, Diogo Contente, Rosa Capita, Carlos Alonso-Calleja, Isabel Carvalho, Jose L. Capelo, Gilberto Igrejas, Patricia Poeta
Summary: The study aimed to characterize the antimicrobial resistance and clonal lineages of MRSP isolated from canine cutaneous pyoderma. The majority of isolates belonged to ST123 and exhibited multidrug resistance, posing a significant threat to animal health.
Article
Microbiology
Bojan Papic, Majda Golob, Irena Zdovc, Darja Kusar, Jana Avbersek
Summary: Staphylococcus pseudintermedius is a common cause of skin infections in dogs, cats, and humans, with the frequency of MRSP strains increasing worldwide. A study in Slovenia found multiple MRSP transmission routes and highlighted the importance of MRSP-positive dogs in veterinary care-associated infections.
VETERINARY MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Eaftekhar Ahmed Rana, Md Zohorul Islam, Tridip Das, Avijit Dutta, Abdul Ahad, Paritosh Kumar Biswas, Himel Barua
Summary: The prevalence of methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in dogs in Bangladesh is concerning, with a high level of resistance to several antibiotics. Dogs with dermatitis, a history of antibiotic use, otitis, and oral lesions are at a higher risk of carrying MRSA and MRSP.
VETERINARY MEDICINE AND SCIENCE
(2022)
Article
Infectious Diseases
Andrea T. Fessler, Anissa D. Scholtzek, Angela R. Schug, Barbara Kohn, Christiane Weingart, Anne-Kathrin Schink, Astrid Bethe, Antina Luebke-Becker, Stefan Schwarz
Summary: A study of 114 Staphylococcus isolates from companion animals revealed variations in susceptibility to antimicrobial agents, with a proportion of isolates carrying resistance genes. However, no signs of biocide resistance were detected at present.
Article
Medicine, General & Internal
Sang Guen Kim, Sib Sankar Giri, Saekil Yun, Sang Wha Kim, Se Jin Han, Jun Kwon, Woo Teak Oh, Sung Bin Lee, Yong Ho Park, Se Chang Park
Summary: This study characterized antimicrobial resistance in Staphylococcus pseudintermedius from canine samples, and isolated two novel bacteriophages, pSp-J and pSp-S, from canine pet parks in South Korea with potential to control methicillin-resistant S. pseudintermedius. The phages showed stability and efficacy in preventing and degrading biofilms at different doses, demonstrating their potential for treating this pathogen.
FRONTIERS IN MEDICINE
(2021)
Article
Immunology
Camilla M. Pesset, Carolina O. da Fonseca, Milena Antunes, Ana Luiza L. dos Santos, Izabel M. Teixeira, Tainara A. N. Ribeiro, Daniela Sachs, Bruno Penna
Summary: This study evaluated the biofilm formation capacity of Staphylococcus pseudintermedius collected from dogs with topical infections on commonly used companion animal surgery suture materials. Results showed that cotton sutures and polyglactin 910 were the most susceptible to biofilm formation. Polypropylene and nylon sutures also demonstrated biofilm formation, albeit to varying extents. PCR analysis confirmed the presence of two biofilm-associated genes in all isolates.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Norma Fabregas, Daniel Perez, Joaquim Vines, Anna Cusco, Lourdes Migura-Garcia, Lluis Ferrer, Olga Francino
Summary: Through long-read Nanopore sequencing, we obtained 67 complete circular genomes and 19 associated plasmids of Staphylococcus pseudintermedius from the skin of 9 healthy dogs. The study revealed the diversity and heterogeneity of S. pseudintermedius populations in healthy dogs' skin, with 39% of them being methicillin-resistant. These findings provide important insights for future control of skin infections and antibiotic surveillance in veterinary medicine.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Jung-Hun Kang, Cheol-Yong Hwang
Summary: The study investigated the genetic relationship of SCCmec between staphylococci isolated from dogs with pyoderma and their owners, revealing a pair with isolates carrying the same SCCmec type V and dru type 11a. This highlights the importance of continuous surveillance of antimicrobial resistance using the One Health concept.
VETERINARY MICROBIOLOGY
(2021)
Article
Biology
Usasom Khongsri, Peerawit Chongrattanameteekul, Sineenart Chantarachart, Kornravee Photichai, Nittaya Chanayat, Thanya Varinrak, Raktham Mektrirat, Nuttawan Srifawattana
Summary: The prevalence of MRSP causing pyoderma is changing gradually, with annual variations. Research on cotrimazole susceptibility to MRSP is limited, but the empirical use of cotrimazole remains interesting. This study aimed to evaluate the susceptibility of cotrimazole to canine pyoderma MRSP isolates.
Article
Multidisciplinary Sciences
Flavia Mello Viegas, Jordana Almeida Santana, Brendhal Almeida Silva, Rafael Gariglio Clark Xavier, Claudia Teixeira Bonisson, Julia Lara Sette Camara, Mario Cesar Renno, Joao Luis Reis Cunha, Henrique Cesar Pereira Figueiredo, Francisco Carlos Faria Lobato, Rodrigo Otavio Silveira Silva
Summary: Staphylococcus pseudintermedius (MRSP) is a commensal bacterium commonly found on the skin and mucosae of dogs, but can also cause various infections in both animals and humans. This study aimed to identify the occurrence of MRSP in infected patients, assess its antimicrobial resistance, and analyze its molecular structure. The findings showed a high incidence of multidrug-resistant MRSP in infected dogs, with surgical site infections being the most common. The study also revealed the dispersion of a particular sequence type associated with fluoroquinolone resistance, raising concerns for animal and human health due to limited treatment options for MRSP infections.
Article
Zoology
Hazel Tamakan, Huban Gocmen
Summary: This research investigated the presence and frequency of Staphylococcus pseudintermedius in cats and dogs with pyoderma and otitis externa, and confirmed methicillin resistance. The study found that methicillin-resistant Staphylococcus pseudintermedius is present in cats and dogs in Cyprus, highlighting the global issue of increasing methicillin resistance.
PAKISTAN JOURNAL OF ZOOLOGY
(2022)
Article
Veterinary Sciences
D. A. Wilkinson, L. E. Rogers, A. Bell, J. Benschop, A. C. Midwinter
Summary: This study investigated the frequency of methicillin-susceptible and methicillin-resistant Staphylococcus pseudintermedius (MRSP) carriage in a population of clinically normal dogs in the Christchurch and wider Canterbury region. The majority of dogs carried S. pseudintermedius, but none carried MRSP. Resistance to penicillin and tetracycline was observed.
NEW ZEALAND VETERINARY JOURNAL
(2023)
Article
Immunology
Dorothea Buensow, Eshraq Tantawy, Tjorven Ostermeier, Heike Baehre, Annette Garbe, Jesper Larsen, Volker Winstel
Summary: MRSP, an emerging zoonotic pathogen of canine origin, relies on specific enzymes NucB and AdsA for pathogenesis, leading to selective killing of host immune cells through a specific pathway, which may aid in the development of new therapeutic strategies.
Article
Psychology, Developmental
Ulrike Ravens-Sieberer, Anne Kaman, Michael Erhart, Christiane Otto, Janine Devine, Constanze Loeffler, Klaus Hurrelmann, Monika Bullinger, Claus Barkmann, Nico A. Siegel, Anja M. Simon, Lothar H. Wieler, Robert Schlack, Heike Hoelling
Summary: The German COPSY study is a longitudinal study that examines the mental health impact of the COVID-19 pandemic on children and adolescents. The study found that during the pandemic, the health-related quality of life (HRQoL) of children and adolescents decreased, and there was an increase in emotional problems, peer-related mental health problems, anxiety, depressive symptoms, and psychosomatic complaints. Socially disadvantaged children and children of mentally burdened parents were more at risk of impaired mental health, while female gender and older age were associated with fewer mental health problems. A positive family climate and social support supported the mental health of children and adolescents during the pandemic.
EUROPEAN CHILD & ADOLESCENT PSYCHIATRY
(2023)
Article
Public, Environmental & Occupational Health
Andre Hajek, Freia De Bock, Christina Merkel, Sarah Eitze, Cornelia Betsch, Michael Bosnjak, Lothar H. Wieler, Hans-Helmut Koenig
Summary: This study aimed to shed light on attitudes towards influenza vaccination in Germany in 2021/2022 based on the COSMO survey. The results showed that about one-third of respondents and healthcare workers plan to get a flu shot this year. Among the at-risk group of people aged 60 and older, more than half plan to get vaccinated. Correlates, such as gender, were identified, with women having a lower likelihood of planning to get a flu shot. The study concludes that physicians should inform women, especially during the pandemic, about the advantages of influenza vaccination and communicate the proven protective effect convincingly.
Article
Infectious Diseases
Anne-Kathrin Schink, Dennis Hanke, Torsten Semmler, Nicole Roschanski, Stefan Schwarz
Summary: The genetic organization of acquired resistance genes in a G. anatis isolate from a German calf with a respiratory tract infection was analyzed. The isolate showed elevated MIC values for various antimicrobial agents and contained multiple resistance genes associated with IS elements. Nucleotide exchanges in gyrA and parC resulting in amino acid exchanges were also identified. The identification of such isolates with reduced susceptibility to commonly used antimicrobial agents is concerning and highlights the potential role as reservoirs for antimicrobial resistance genes.
Article
Biology
Sumeet K. Tiwari, Boas C. L. van der Putten, Thilo M. Fuchs, Trung N. Vinh, Martin Bootsma, Rik Oldenkamp, Roberto La Ragione, Sebastien Matamoros, Ngo T. Hoa, Christian Berens, Joy Leng, Julio Alvarez, Marta Ferrandis-Vila, Jenny M. Ritchie, Angelika Fruth, Stefan Schwarz, Lucas Dominguez, Maria Ugarte-Ruiz, Astrid Bethe, Charlotte Huber, Vanessa Johanns, Ivonne Stamm, Lothar H. Wieler, Christa Ewers, Amanda Fivian-Hughes, Herbert Schmidt, Christian Menge, Torsten Semmler, Constance Schultsz
Summary: This study analyzes the genetic determinants of host specificity in E. coli and identifies specific genes associated with different hosts. The nan-9 gene cluster shows a strong association with the human host, while previously known sialic acid regulon genes show no significant association with any host.
Article
Public, Environmental & Occupational Health
Simone Scheithauer, Alexander Dilthey, Anna Bludau, Sandra Ciesek, Victor Corman, Tjibbe Donker, Tim Eckmanns, Richard Egelkamp, Hajo Grundmann, Georg Haecker, Martin Kaase, Berit Lange, Alexander Mellmann, Martin Mielke, Mathias Pletz, Bernd Salzberger, Andrea Thuermer, Andreas Widmer, Lothar H. Wieler, Thorsten Wolff, Soeren Gatermann, Torsten Semmler
Summary: The deficit of genomic pathogen surveillance infrastructure in Germany has been revealed during the SARS-CoV-2 pandemic. Establishing an efficient and adaptable genomic pathogen surveillance network is urgently needed to prepare for future pandemics. This paper proposes measures based on global and country-specific best practices to achieve integrated genomic pathogen surveillance, which includes linking epidemiological data with pathogen genomic data, sharing and coordinating resources, making surveillance data available to decision-makers, public health service, and the scientific community, and engaging all stakeholders.
BUNDESGESUNDHEITSBLATT-GESUNDHEITSFORSCHUNG-GESUNDHEITSSCHUTZ
(2023)
Article
Public, Environmental & Occupational Health
Christopher Irrgang, Tim Eckmanns, Max von Kleist, Esther-Maria Antao, Katharina Ladewig, Lothar Wieler, Nils Koerber
Summary: Societal health is facing new challenges due to climate change, demographic ageing, and globalization. The One Health approach aims to understand health holistically by linking human, animal, and environmental sectors. Artificial intelligence techniques offer opportunities for cross-sectoral assessment of health threats. This article discusses the applications and challenges of AI techniques in the context of One Health, using antimicrobial resistance as an example.
BUNDESGESUNDHEITSBLATT-GESUNDHEITSFORSCHUNG-GESUNDHEITSSCHUTZ
(2023)
Article
Infectious Diseases
Bernd Salzberger, Alexander Mellmann, Anna Bludau, Sandra Ciesek, Victor Corman, Alexander Dilthey, Tjibbe Donker, Tim Eckmanns, Richard Egelkamp, Soeren G. Gatermann, Hajo Grundmann, Georg Haecker, Martin Kaase, Berit Lange, Martin Mielke, Mathias W. Pletz, Torsten Semmler, Andrea Thuermer, Lothar H. Wieler, Thorsten Wolff, Andreas F. Widmer, Simone Scheithauer
Summary: The COVID-19 pandemic has highlighted the importance of effective infection surveillance and infrastructure. Germany's genomic pathogen sequencing infrastructure was inadequate due to a lack of equipment, resources, data management, and coordination. The authors propose the establishment of comprehensive genomic pathogen surveillance in Germany, including sequencing, data collection, data linkage, and target pathogens, to better address future epidemics and pandemics and learn from the lessons of the COVID-19 pandemic.
Article
Public, Environmental & Occupational Health
Carmen Koschollek, Katja Kajikhina, Charbel El Bcheraoui, Lothar H. Wieler, Niels Michalski, Claudia Hovener
Summary: The study aimed to investigate the risk of SARS-CoV-2 infection among migrants and ethnic minorities in Germany, and discuss potential explanations for these associations. The results showed that the risk of SARS-CoV-2 infection is determined by socio-economic factors rather than migrant status.
Article
Microbiology
Ruth Offergeld, Karina Preussel, Thomas Zeiler, Konstanze Aurich, Barbara I. Baumann-Baretti, Sandra Ciesek, Victor M. Corman, Viktoria Dienst, Christian Drosten, Siegfried Goerg, Andreas Greinacher, Marica Grossegesse, Sebastian Haller, Hans-Gert Heuft, Natalie Hofmann, Peter A. Horn, Claudia Houareau, Ilay Guelec, Carlos Luis Jimenez Klingberg, David Juhl, Monika Lindemann, Silke Martin, Hannelore K. Neuhauser, Andreas Nitsche, Julia Ohme, Sven Peine, Ulrich J. Sachs, Lars Schaade, Richard Schaefer, Heinrich Scheiblauer, Martin Schlaud, Michael Schmidt, Markus Umhau, Tanja Vollmer, Franz F. Wagner, Lothar H. Wieler, Hendrik Wilking, Malte Ziemann, Marlow Zimmermann, Matthias an der Heiden
Summary: SARS-CoV-2 serosurveillance is crucial for infection control measures and understanding underreporting. A study in Germany analyzed blood donor samples collected from April 2020 to April 2021, September 2021, and April/May 2022. The seroprevalence of SARS-CoV-2 increased over time, reaching 100% in April/May 2022. Neutralizing capacity was also observed in the majority of positive specimens. The study revealed low levels of underreporting, indicating an effective testing and notification system in Germany.
Article
Medical Informatics
Anatol-Fiete Naeher, Carina N. Vorisek, Sophie A. Klopfenstein, Moritz Lehne, Sylvia Thun, Shada Alsalamah, Sameer Pujari, Dominik Heider, Wolfgang Ahrens, Iris Pigeot, Georg Marckmann, Mirjam A. Jenny, Bernhard Y. Renard, Max von Kleist, Lothar H. Wieler, Felix Balzer, Linus Grabenhenrich
Summary: Substantial opportunities for global health intelligence and research can be achieved through the combined and optimised use of secondary data within data ecosystems. This includes data from emerging sources such as the internet, wearables, mobile phone apps, electronic health records, and genome sequencing. Guidance on available sources and approaches for processing secondary data, as well as criteria for assessing reusability, can support policy decision making and improve early detection and prevention of emerging health threats.
LANCET DIGITAL HEALTH
(2023)
Article
Public, Environmental & Occupational Health
Benjamin F. Maier, Annika H. Rose, Angelique Burdinski, Pascal Klamser, Hannelore Neuhauser, Ole Wichmann, Lars Schaade, Lothar H. Wieler, Dirk Brockmann
Summary: After the winter of 2021/2022, a considerable number of people in Germany have been infected with or vaccinated against the SARS-CoV-2 variant. However, due to under-reporting and unknown overlap between the vaccinated and recovered populations, the exact extent is difficult to estimate. This study provides estimates of the share of immunologically naive individuals in each age group for the German population by integrating infectious-disease modeling and vaccine uptake data. The study shows that a significant proportion of the population in Germany remains immunologically naive, highlighting the impact of the Omicron waves.
EPIDEMIOLOGY AND INFECTION
(2023)
Article
Veterinary Sciences
Dordia Anindita Rotinsulu, Christa Ewers, Katharina Kerner, Amrozi Amrozi, Retno Damayanti Soejoedono, Torsten Semmler, Rolf Bauerfeind
Summary: This study aimed to characterize S. equi equi isolates obtained from suspected strangles cases in Indonesia in 2018. The results showed that all isolates belonged to ST179 and carried seM allele 166. The isolates differed from each other by only 2 to 14 cgSNPs and formed an exclusive sub-cluster within the BAPS-2 of S. equi equi cgMLST scheme. Furthermore, all isolates were susceptible to beta-lactam antibiotics penicillin G, ampicillin, and ceftiofur.
VETERINARY SCIENCES
(2023)
