Article
Veterinary Sciences
Peixun Li, Yesheng Shen, Tailong Wang, Jing Li, Yan Li, Yiran Zhao, Sidang Liu, Baoquan Li, Mengda Liu, Fanliang Meng
Summary: Mutations have regularly occurred since the rise of porcine reproductive and respiratory syndrome virus (PRRSV) in China, particularly the emergence of the highly pathogenic HP-PRRSV. This study investigated the prevalence and evolution of PRRSV in Shandong Province through the analysis of genetic variation in the isolated PRRSV ORF5 gene. The dominant strain in the province is still NADC30-like, but the emergence of NADC34-like strain is notable. Mutations in certain amino acids can affect virulence and immune escape ability of the isolates.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Qin Luo, Yajie Zheng, Yingxin He, Gan Li, Hang Zhang, Huiyang Sha, Zhiqing Zhang, Liangzong Huang, Mengmeng Zhao
Summary: PRRSV has been prevalent in China for more than 25 years and poses a serious threat to the pig industry. Genetic analysis of PRRSV-2 strains revealed nucleotide and amino acid variations, with some mutations found in key regions of the GP5 and GP5a proteins. Phylogenetic analysis classified the prevalent PRRSV strains in China into lineages 1, 3, 5, and 8, with recombination events identified between lineages 8 and 1. This study enhances our understanding of PRRSV-2 prevalence and genetic variation in China, providing insights for prevention and control strategies.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Virology
Xuewei Liu, Xing Liu, Juan Bai, Yanni Gao, Zhongbao Song, Hans Nauwynck, Xianwei Wang, Yuanqi Yang, Ping Jiang
Summary: This study unveils the interaction between PRRSV GP5 and GAPDH, demonstrating that GP5 binds to a 13-amino-acid sequence on GP5 and interacts with GAPDH at the K277 residue. The findings shed light on the role of GAPDH in PRRSV replication and present a potential target for controlling viral infection.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Lujia Zhou, Yang Yang, Qiqi Xia, Zhixin Guan, Junjie Zhang, Beibei Li, Yafeng Qiu, Ke Liu, Donghua Shao, Zhiyong Ma, Xiaodu Wang, Jianchao Wei
Summary: PRRS is an immunosuppressive disease in pigs caused by PRRSV, leading to reproductive and respiratory disorders. The predominant strain in Eastern China is still highly pathogenic PRRSV, with an increasing proportion of NADC30-like and NADC34-like strains. This study enriches the epidemiological data and provides a theoretical basis for the development of vaccines and prevention and control of the disease.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Pandeng Zhao, Linyang Yu, Yanling Liu, Leyi Zhang, Pengshuai Liang, Lei Wang, Huiyuan Jing, Li Huang, Changxu Song, Jianguo Dong
Summary: This study isolated 43 PRRSV strains from the lungs of sick pigs, with analysis revealing that lineage 8 (8.7) subgroup III strains of PRRSV2 remain dominant in South China. Novel deletions and mutations were identified in the Nsp2 and GPS genes. Additionally, frequent recombination events between different lineages of PRRSV were observed in Guangdong Province.
Article
Veterinary Sciences
Hwi-Yeon Choi, Min-Sik Kim, Yeong-Lim Kang, Jong-Chul Choi, In-Yeong Choi, Sung-Won Jung, Ji-Yun Jeong, Min-Chul Kim, Seong-Soo Hwang, Sang-Won Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee
Summary: In this study, a vaccine candidate against Korean lineage-1 PRRSV was developed using reverse genetics technology. The vaccine showed high serum virus neutralization antibody titers in an animal experiment, indicating its potential as a promising vaccine candidate.
VETERINARY SCIENCES
(2022)
Article
Immunology
Guanyu Zhao, Jiaqi Zhang, Wenchao Sun, Changzhan Xie, He Zhang, Yan Gao, Shubo Wen, Zhuo Ha, Fulong Nan, Xiangyu Zhu, Sheng Feng, Xinyu Cao, Ying Zhang, Yanzhu Zhu, Ningyi Jin, Huijun Lu
Summary: This study examined the immunological effects of DNA vaccines with three adjuvants in pigs challenged with PRRSV. The results showed that the use of adjuvant A3 led to higher levels of neutralizing antibodies and a lower viral load in pigs compared to the other adjuvants.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Xiaoyu Cao, Xinna Ge, Yongning Zhang, Xin Guo, Jun Han, Lei Zhou, Hanchun Yang
Summary: Three novel PRRSV strains were isolated and found to cluster with NADC30-like virus, with a specific deletion in the nsp2 coding region and variable mutagenesis in the GP2 to GP5 antigenic sites. Recombination analysis revealed that these strains were generated through multiple recombination events involving NADC30-like virus, HP-PRRSV vaccine-like virus, and QYYZ-like virus. Furthermore, QYYZ-like strains were found to undergo frequent recombination and obtain immunogenic and protective protein fragments from QYYZ.
TRANSBOUNDARY AND EMERGING DISEASES
(2023)
Article
Infectious Diseases
Jiankui Liu, Ye Xu, Zhifeng Lin, Jialin Fan, Ailing Dai, Xiaoying Deng, Wan Mao, Xiaozi Huang, Xiaoyan Yang, Chunhua Wei
Summary: The study conducted in China from 2017 to 2019 showed that Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) can be transmitted via faeces between farms, with phylogenetic analysis revealing four main lineages.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Immunology
Yaozhong Ding, Ashenafi Kiros Wubshet, Xiaolong Ding, Zhongwang Zhang, Qian Li, Junfei Dai, Qian Hou, Yonghao Hu, Jie Zhang
Summary: This study evaluated the efficacy of four PRRSV commercial vaccines against the QH-08 challenge, showing that JXA1 and JXA1-R MLV vaccines provided complete protection, while Ingelvac PRRS MLV and CH-1 alpha vaccines only offered partial protection. The effectiveness of these vaccines was found to be significantly influenced by variations in ORF1a and 1b coding sequences.
Article
Virology
Yu Wu, Ouyang Peng, Qiuping Xu, Qunhui Li, Wei Li, Limiao Lin, Qingfeng Zhou, Xinbin Cai, Guangli Hu, Zuyong He, Yaosheng Chen, Hao Zhang
Summary: This study isolated and identified two novel recombinant PRRSV strains, named GD-H1 and GD-F1, from pig farms in Guangdong province, China. These recombinant strains showed high pathogenicity to sows and provided early warning and support for the immune prevention and control of porcine reproductive and respiratory syndrome (PRRS).
Article
Virology
Shuo Li, Mengxia Ye, Yonglin Chen, Quan Gong, Bing Mei
Summary: This study sheds light on the relationship between HPV-52 and cervical cancer, identifying variations in the E6-E7 genes and analyzing the protein's structure and selective pressure. Multiple single nucleotide variants were found in the E6 and E7 sequences of HPV-52, some impacting B cell epitopes.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Veterinary Sciences
Giovani Trevisan, Aditi Sharma, Phillip Gauger, Karen M. Harmon, Jianqiang Zhang, Rodger Main, Michael Zeller, Leticia C. M. Linhares, Daniel C. L. Linhares
Summary: The genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) varies over time and geographic space in the U.S. based on ISU-VDL data from 2007 to 2019. Analysis of 40,454 ORF5 sequences revealed significant differences within RFLP families, with certain RFLP types showing distinct distribution trends and clusters, aiding in the development of regional control programs and animal movement policies to minimize PRRSV transmission risk.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2021)
Article
Biology
Fu-Chun Hsueh, Kun-Lin Kuo, Feng-Yang Hsu, Sheng-Yuan Wang, Hsien-Jen Chiu, Meng-Tien Wu, Chuen-Fu Lin, Yu-Han Huang, Ming-Tang Chiou, Chao-Nan Lin
Summary: Two variants of porcine reproductive and respiratory syndrome virus (PRRSV), PRRSV 1 and PRRSV 2, have caused reproductive and respiratory problems in pigs worldwide. This study focused on PRRSV 1 in Taiwan and found that its genetic characteristics are closely related to Spanish-like strains. Analysis showed that there are specific codon mutations in the ORF5 gene of PRRSV 1, which have implications for vaccine development and disease prevention.
Article
Biodiversity Conservation
Andres Baselga, Carola Gomez-Rodriguez, Miguel B. Araujo, Adrian Castro-Insua, Miguel Arenas, David Posada, Alfried P. Vogler
Summary: This study developed a predictive framework based on approximate Bayesian computation to quantify the role of dispersal and environmental constraints in community turnover. Simulations showed that spatial turnover rates remain invariant across genealogical scales when dispersal limitation determines species ranges, but vary when environmental constraint limits species ranges. Analysis of empirical biological communities revealed a combination of dispersal and environmental constraints influencing spatial turnover at different scales. The study highlights the importance of considering multiple genealogical scales in understanding the relative role of dispersal and environmental constraints in community turnover.