Article
Agriculture, Dairy & Animal Science
Maria Victoria Miro, Livio Martins Costa-Junior, Mercedes Lloberas, Patricia Cardozo, Carlos Lanusse, Guillermo Virkel, Adrian Lifschitz
Summary: In the past 50 years, the limited incorporation of modern drugs with new mechanisms of action has led to an increase in drug resistance worldwide. Therefore, finding alternative pharmacological tools is a priority in ruminant production systems. Natural products have historically played a relevant role in drug discovery. Although many phytochemicals have shown anthelmintic activity in vitro, their in vivo pharmacological properties have not been extensively explored. This study aimed to evaluate the combined administration of ivermectin and carvone to lambs infected with resistant nematodes and found that innovative pharmaceutical formulations are needed to fully utilize the anthelmintic activity of phytochemicals.
Article
Multidisciplinary Sciences
Gerwin Louis T. Dela Torre, Alice Alma C. Bungay
Summary: This study investigated the effect of probiotic Lactobacillus acidophilus on the transepithelial mucosal-to-serosal transport of colchicine. The results showed that Lactobacillus acidophilus BIOTECH 1900 reduced the transport of colchicine, suggesting a potential induction of P-gp.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
Article
Chemistry, Medicinal
Omar Y. Mady, Adam A. Al-Shoubki, Ahmed A. Donia
Summary: The study demonstrated that sorbitan monostearate, used as a granulating agent for metformin HCl, enhanced drug permeability and reduced blood glucose levels in diabetic rats. The research indicated a dual enhancement effect of the suggested industrial procedure.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Pharmacology & Pharmacy
Rong Li, Yuwen Chen, Mi Jia, Xuehua Jiang, Ling Wang
Summary: In this study, the pharmacokinetic properties and absorption mechanisms of Tandospirone citrate (TDS) were investigated using in vivo, in vitro, and ex vivo approaches. The results showed that TDS was rapidly eliminated in rats and extensively metabolized. TDS exhibited good permeability in the Caco-2 cell model and its transport was influenced by concentration, temperature, and pH. Passive diffusion was identified as the main absorption mechanism.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Applied
Man-Man Yu, Ying-Chen Fan, De-Yang Li, Yu-Xin Liu, Peng-Fei Jiang, Da-Yong Zhou, Bei-Wei Zhu
Summary: This study investigated the texture and digestive properties of different parts of abalone muscle (adductor and transition part) boiled at 80 degrees Celsius for varying times. The shear force and hardness of the adductor initially increased and then decreased with longer boiling times, while these indices dramatically decreased for the transition part after boiling for 6 minutes and remained stable until 240 minutes. The degree of protein hydrolysis, digestibility, and peptide transport levels decreased with longer boiling times for the adductor, while these protein digestion and transport indices initially decreased (6 minutes and 30 minutes) and then increased (240 minutes) for the transition part.
Article
Pharmacology & Pharmacy
Nahathai Dukaew, Patcharawadee Thongkumkoon, Nutnicha Sirikaew, Sivamoke Dissook, Wannachai Sakuludomkan, Siripong Tongjai, Parameth Thiennimitr, Mingkwan Na Takuathung, Juthipong Benjanuwattra, Prachya Kongthaweelert, Nut Koonrungsesomboon
Summary: This study aimed to investigate the pharmacokinetic drug-drug interactions between MPA and TMP-SMX, as well as the relationship between MPA pharmacokinetics and gut microbiota alteration. The results showed a significant decrease in systemic MPA exposure when TMP-SMX was coadministered with MMF. Analysis of the gut microbiome revealed altered relative abundance of certain genera, including Bacteroides and Faecalibacterium, which correlated with systemic MPA exposure.
Article
Veterinary Sciences
Esther Blanco-Paniagua, Laura Alvarez-Fernandez, Alicia Millan-Garcia, Guillermo Rivas, Ana I. Alvarez, Gracia Merino
Summary: In the veterinary field, drug exposure during milk production in dairy cattle is a major health concern. This study found that coadministration of ABCG2 inhibitors affects the milk pharmacokinetics of clorsulon.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Chemistry, Applied
Xinmiao Wang, Qian Wang, Yuanyuan Hu, Fawen Yin, Xiaoyang Liu, Dayong Zhou
Summary: The hydrolysis and transport characteristics of tyrosol-SCFA esters (TYr-SEs) were studied using rat everted gut sac and rat plasma hydrolysis models. Results showed that TYr-SEs could be hydrolyzed by intestinal lipase, leading to sustained release of SCFAs and tyrosol. TYr-SEs and the liberated SCFAs and tyrosol could cross the membrane and enter the blood circulation. Carboxylesterase in plasma further hydrolyzed TYr-SEs, resulting in sustained release of SCFAs and tyrosol. The hydrolysis and transport rates were found to be influenced by the acyl chain lengths, with higher rates observed for TYr-SEs with straight chains than for its isomer with branched chains. This two-step release of SCFAs and tyrosol demonstrated that TYr-SEs could effectively enhance the health benefits of SCFAs and tyrosol.
Article
Parasitology
Tania Rojas-Campos, Froylan Ibarra-Velarde, Yolanda Vera-Montenegro, Miguel Flores-Ramos, Irene Cruz-Mendoza, Gerardo Leyva-Gomez, Alicia Hernandez-Campos
Summary: This study compared the fasciolicidal efficacy of a new injectable formulation of fosfatriclaben with subcutaneous closantel and oral triclabendazole. Results showed that fosfatriclaben and triclabendazole had similar efficacy, but fosfatriclaben's intramuscular administration was more convenient and had lower dosage.
VETERINARY PARASITOLOGY
(2021)
Article
Food Science & Technology
Min Zhang, Yuxin Liu, Mengling Jin, Deyang Li, Ziye Wang, Pengfei Jiang, Dayong Zhou
Summary: This study investigated the effect of oxidation on the digestion and absorption properties of protein in boiled sea cucumber body wall. Boiling heat treatments led to protein oxidation, which affected various properties including structure, hydrophobicity, and aggregation. Boiling for 2 hours improved protein digestion and absorption levels, while excessive oxidative modification decreased these levels. Boiling for 2 hours is considered to be a better processing condition for sea cucumber products.
Article
Agronomy
Lenka Langhansova, Martina Navratilova, Lenka Skalova, Katerina Mot'kova, Radka Podlipna
Summary: A study was conducted on clover fertilized with sheep manure treated with anthelmintics ABZ, IVM, and MOP, revealing that ABZ and MOP were absorbed, metabolized, and translocated to leaves, while IVM was only detected in roots with minimal impact on plant stress.
Article
Pharmacology & Pharmacy
Jaime Algorta, Alejandro Krolewiecki, Filipe Pinto, Silvia Gold, Jose Munoz
Summary: This study aimed to evaluate the pharmacokinetic characteristics and bioavailability of a fixed-dose combination of ivermectin and albendazole. The results showed bioequivalence between the fixed combination and ivermectin, but not with albendazole. All three medications were well tolerated.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Microbiology
Mariel Fulham, Michelle Power, Rachael Gray
Summary: The gut microbiota of endangered Australian sea lion pups was studied before and after topical ivermectin treatment for hookworm infection. The treatment did not alter the composition of the gut microbiota, indicating its safety and minimal invasiveness. This study provides important insights into the gut microbiota of free-ranging pinniped pups and the effects of parasitic treatment.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Esther Blanco-Paniagua, Alba M. Garcia-Lino, Laura Alvarez-Fernandez, Ana Alvarez, Gracia Merino
Summary: The study confirms the role of ABCG2 transporter protein in the secretion of the non-steroidal anti-inflammatory drug meloxicam into milk, and demonstrates that ivermectin, as an inhibitor of ABCG2, can reduce the concentration of meloxicam in milk.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Medicinal
Jorge Errecalde, Adrian Lifschitz, Graciela Vecchioli, Laura Ceballos, Francisco Errecalde, Mariana Ballent, Gustavo Marin, Martin Daniele, Esteban Turic, Eduardo Spitzer, Fernando Toneguzzo, Silvia Gold, Alejandro Krolewiecki, Luis Alvarez, Carlos Lanusse
Summary: The recent study in a pig model evaluated the safety and pharmacokinetic performance of a novel IVM nasal spray, showing potential for achieving high drug concentrations in nasopharyngeal tissue and good animal tolerability without adverse events. The nasal administration route resulted in the highest IVM concentrations in nasopharyngeal and lung tissues, indicating advantages over oral administration in attaining high and persistent drug levels in target tissues, supporting the future clinical evaluation of nasal IVM spray in COVID-19 prevention and treatment.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Chemistry, Medicinal
Jorge Errecalde, Adrian Lifschitz, Graciela Vecchioli, Laura Ceballos, Francisco Errecalde, Mariana Ballent, Gustavo Marin, Martin Daniele, Esteban Turic, Eduardo Spitzer, Fernando Toneguzzo, Silvia Gold, Alejandro Krolewiecki, Luis Alvarez, Carlos Lanusse
Summary: The recent study in a pig model evaluated the safety and pharmacokinetic performance of a novel IVM nasal spray, showing potential for achieving high drug concentrations in nasopharyngeal tissue and good animal tolerability without adverse events. The nasal administration route resulted in the highest IVM concentrations in nasopharyngeal and lung tissues, indicating advantages over oral administration in attaining high and persistent drug levels in target tissues, supporting the future clinical evaluation of nasal IVM spray in COVID-19 prevention and treatment.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Parasitology
S. Luque, M. Lloberas, P. Cardozo, G. Virkel, C. Farias, P. Viviani, C. Lanusse, L. Alvarez, A. Lifschitz
Summary: The combination therapy of MOX and LEV showed higher anthelmintic effect compared to using either drug alone, proving to be an effective strategy in controlling multi-resistant gastrointestinal nematodes over a four-year study period.
VETERINARY PARASITOLOGY
(2021)
Article
Pharmacology & Pharmacy
Lucila Canton, Candela Canton, Laura Ceballos, Paula Dominguez, Jose Rodriguez, Carlos Lanusse, Luis Alvarez, Laura Moreno
Summary: This experimental work replicated the extra-label use of fipronil by producers in laying hens, with two different administration routes: feed additives and topical administration. The study found that the residual concentrations of fipronil in eggs varied significantly between the two treatment methods, suggesting the importance of withdrawal periods to avoid risks to consumers.
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
(2021)
Article
Parasitology
Candela Canton, Lucila Canton, Adrian Lifschitz, Maria Paula Dominguez, Juan Torres, Carlos Lanusse, Luis Alvarez, Laura Ceballos, Mariana Ballent
Summary: The study aimed to evaluate the pharmacokinetics and pharmacodynamics of monepantel in calves naturally infected with GI nematodes resistant to ivermectin and ricobendazole. Results showed that monepantel had high efficacy against the resistant nematodes and could be a valuable tool to manage nematode resistance.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2021)
Article
Microbiology
L. Ceballos, E. Nieves, M. Juarez, R. Aveldano, M. Travacio, J. Martos, R. Cimino, J. L. Walson, A. Krolewiecki, C. Lanusse, L. Alvarez
Summary: This study evaluated the impact of diet and fasting on the efficacy of albendazole treatment, as well as the potential feasibility of detecting albendazole metabolites in urine samples. The results suggest that food intake can enhance the availability of the active ALB-sulfoxide metabolite, and that urine analysis could be used as a noninvasive tool to evaluate adherence to ALB treatment.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Pharmacology & Pharmacy
Mariana Ballent, Candela Canton, Paula Dominguez, Laura Mate, Laura Ceballos, Carlos Lanusse, Adrian Lifschitz
Summary: This study found that after dose normalization, the concentration differences of EPM in plasma and milk were not significant. However, Cmax and AUC values were significantly affected by the dose of 1.5 mg/kg, showing non-linear patterns. The milk-to-plasma ratio of EPM was low, indicating a low percentage of the administered dose excreted through milk.
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
(2022)
Article
Parasitology
Laura Mate, Mariana Ballent, Candela Canton, Carlos Lanusse, Laura Ceballos, Luis L. Ignacio Alvarez, Juan Pedro Liron
Summary: This study compared the gene expression levels of ABC transporters in sheep nematode Haemonchus contortus isolates with different susceptibility to ivermectin and found that some ABC transporter genes showed minor differences in transcription levels, but could not fully explain ivermectin resistance. Pgp-9 may be one of the most relevant candidates contributing to the multi-genic nature of ivermectin resistance.
VETERINARY PARASITOLOGY
(2022)
Article
Chemistry, Applied
Karen Larsen, Adrian Lifschitz, Rocio Fernandez San Juan, Guillermo Virkel
Summary: This study investigated the metabolism of GLY and AMPA in the ruminal environment of cattle and evaluated their distribution in the ruminal content. The results showed that there were no metabolites of GLY and AMPA in the rumen of cattle, and they were mainly present in the fluid phase of the ruminal content. This indicates a high metabolic stability of GLY and AMPA in the ruminal environment, which may result in a rapid flow into the posterior gastrointestinal tract.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Veterinary Sciences
Paula Ichinose, Maria Victoria Miro, Karen Larsen, Carlos Lanusse, Adrian Lifschitz, Guillermo Virkel
Summary: This study evaluated the availability and metabolism of fenbendazole (FBZ) and its metabolites in plasma and liver of pigs, and characterized the effect of continuous administration of FBZ on xenobiotic metabolizing enzyme activities in pig liver. The results showed that continuous administration of FBZ can accelerate its own metabolism through the CYP1A pathway in the liver.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Agriculture, Dairy & Animal Science
Maria Victoria Miro, Livio Martins Costa-Junior, Luis Ignacio Alvarez, Carlos Lanusse, Guillermo Virkel, Adrian Lifschitz
Summary: This study found that Geraniol (GNL) has an anthelmintic effect against gastrointestinal nematodes in sheep, but its pharmacokinetic interaction with albendazole (ABZ) is limited. The results highlight the importance of integrated in vitro/in vivo pharmaco-parasitological studies in developing new pharmacological tools for controlling gastrointestinal parasites.
VETERINARY AND ANIMAL SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Karen Larsen, Paula Ichinose, Rocio Fernandez-San Juan, Adrian Lifschitz, Guillermo Virkel
Summary: This study evaluated the potential inhibitory effects of commonly used acaricides on hepatic enzyme activities in cattle. The results showed that all acaricides, especially phosphorothionate-containing OPs, inhibited multiple enzyme activities to some extent, but with low inhibitory potency. Therefore, the risk of in vivo metabolic interactions due to monooxygenase inhibition is low under common husbandry conditions.
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
(2023)
Article
Agriculture, Dairy & Animal Science
Maria Victoria Miro, Livio Martins Costa-Junior, Mercedes Lloberas, Patricia Cardozo, Carlos Lanusse, Guillermo Virkel, Adrian Lifschitz
Summary: In the past 50 years, the limited incorporation of modern drugs with new mechanisms of action has led to an increase in drug resistance worldwide. Therefore, finding alternative pharmacological tools is a priority in ruminant production systems. Natural products have historically played a relevant role in drug discovery. Although many phytochemicals have shown anthelmintic activity in vitro, their in vivo pharmacological properties have not been extensively explored. This study aimed to evaluate the combined administration of ivermectin and carvone to lambs infected with resistant nematodes and found that innovative pharmaceutical formulations are needed to fully utilize the anthelmintic activity of phytochemicals.
Article
Immunology
Gabriela Matamoros, Ana Sanchez, Jose Antonio Gabrie, Marisa Juarez, Laura Ceballos, Andres Escalada, Carol Rodriguez, Helena Marti-Soler, Maria Mercedes Rueda, Maritza Canales, Carlos Lanusse, Pamela Cajal, Luis Alvarez, Ruben O. Cimino, Alejandro Krolewiecki
Summary: This study demonstrates that the combined use of ALB and high-dose IVM is highly effective and well tolerated for the treatment of Trichuris trichiura infections. Comparisons show that the efficacy of the IVM groups is significantly better than the ALB-only groups.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Medicine, General & Internal
Alejandro Krolewiecki, Adrian Lifschitz, Matias Moragas, Marina Travacio, Ricardo Valentini, Daniel F. Alonso, Ruben Solari, Marcelo A. Tinelli, Ruben O. Cimino, Luis Alvarez, Pedro E. Fleitas, Laura Ceballos, Marcelo Golemba, Florencia Fernandez, Diego Fernandez de Oliveira, German Astudillo, Ines Baeck, Javier Farina, Georgina A. Cardama, Andrea Mangano, Eduardo Spitzer, Silvia Gold, Carlos Lanusse
Summary: This study showed that high-dose oral ivermectin has concentration-dependent antiviral activity against SARS-CoV-2, impacting the viral load in respiratory secretions significantly. There was no significant difference observed in clinical outcomes between the two groups of patients.
Article
Veterinary Sciences
L. Alibrandi, R. Tognetti, O. Domenech, M. Croce, M. Giuntoli, G. Grosso, T. Vezzosi
Summary: This study assessed the feasibility and diagnostic reliability of a new smartphone-based ECG device in dogs, and found no significant differences compared to a traditional ECG device. The results suggest that the smartphone-based device is clinically reliable for assessing heart rate and rhythm in dogs.
VETERINARY JOURNAL
(2024)
