Article
Infectious Diseases
Artur J. Sabat, Erik Bathoorn, Monika A. Chlebowicz-Fliss, Viktoria Akkerboom, Inge Kamphuis, Claudy Oliveira Dos Santos, Alexander W. Friedrich
Summary: This study describes two false-negative results in the detection of meticillin-resistant Staphylococcus aureus (MRSA) of sequence type 398 and spa type t011 using the Cepheid Xpert MRSA NxG assay, indicating that this MRSA strain may have been spreading in the northern Netherlands for some time and could have disseminated to other regions.
Review
Infectious Diseases
Joshua. B. Parsons, Annette. C. Westgeest, Brian. P. B. Conlon, Vance. G. Fowler
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a deadly pathogen that can persist in the bloodstream for days despite appropriate antibiotics. Persistent MRSA bacteremia is common and associated with poor clinical outcomes. This review explores the factors related to host-pathogen interaction and discusses the clinical relevance of each element. Treatment options and diagnostic approaches for managing persistent MRSA bacteremia are also discussed.
Article
Public, Environmental & Occupational Health
Sara Quero, Marina Serras-Pujol, Noemi Parraga-Nino, Carmen Torres, Marian Navarro, Anna Vilamala, Emma Puigoriol, Javier Diez de los Rios, Elisenda Arque, Judit Serra-Pladevall, Alba Romero, Daniel Molina, Roger Paredes, Maria Luisa Pedro-Botet, Esteban Reynaga
Summary: This study investigated the prevalence of methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) among workers in the pork production chain. The results showed that the prevalence of MRSA was high among workers in contact with live animals, especially in pig farms, livestock transporters, and slaughterhouses.
Article
Biochemistry & Molecular Biology
Mark Hutchins, Richard A. Bovill, Peter J. Stephens, John A. Brazier, Helen M. I. Osborn
Summary: The increasing number of antibiotic-resistant bacteria poses a serious threat to global human health. This study examines the glycosylation of nadifloxacin to improve its solubility and finds that one glycoside derivative has the potential to be an effective antibacterial agent.
Article
Microbiology
Alexandra E. Chittams-Miles, Areej Malik, Erin B. Purcell, Claudia Muratori, Brian Conlon
Summary: Treatment with short electric pulses can enhance the effects of multiple antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). This combination therapy reduces the necessary antibiotic dosage and improves the efficacy of treating S. aureus infections such as skin and soft-tissue infections (SSTIs). SSTIs are accessible to physical intervention, making electric pulse co-treatment a potential option for wound and abscess debridement.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Kevin Simon, Wolfgang Pier, Alex Kruettgen, Hans-Peter Horz
Summary: The combination of Sb-1 phage and oxacillin showed enhanced antibacterial effects against most S. aureus isolates, with rare antagonism observed. This heterologous antimicrobial pair provides a robust approach for controlling MRSA.
Article
Biochemistry & Molecular Biology
Thibaut Barbier, Alexia Barbry, Jeremy Magand, Cedric Badiou, Floriane Davy, Anne Baudouin, Yves Queneau, Oana Dumitrescu, Gerard Lina, Laurent Soulere
Summary: In this study, new series of compounds were synthesized by combining the benzo[b]thiophene nucleus and the acylhydrazone functional group for screening against Staphylococcus aureus. Among the compounds tested, (E)-6-chloro-N'-(pyridin-2-ylmethylene)benzo[b]thiophene-2-carbohydrazide (II.b) showed promising antimicrobial activity with a minimal inhibitory concentration of 4 μg/mL against S. aureus strains, including clinically isolated strains resistant to methicillin and daptomycin.
Article
Infectious Diseases
Nicholas M. Brown, Erwin M. Brown
Summary: The UK guidelines for MRSA infection treatment have been updated, incorporating new evidence and antibiotics for Gram-positive bacterial infections. However, the current literature review found a lack of strong evidence, resulting in a hybrid of varying degrees of evidence and expert opinion in the guidelines.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Hyunjung Lee, Jaehoan Lee, Juchan Hwang, Sinyoung Park, Namyoul Kim, Kideok Kim, Honggun Lee, David Shum, Soojin Jang
Summary: The study screened 182 FDA-approved drugs and identified three potential antibiotic candidates against Staphylococcus aureus. Among them, eltrombopag showed the highest antibacterial activity, with significant potency against not only a drug-sensitive S. aureus strain but also 55 clinical isolates including 35 methicillin-resistant S. aureus.
Article
Immunology
Syong H. Nam-Cha, Elena Dominguez-Jurado, Selena L. Tinoco-Valencia, Ramon Perez-Tanoira, Noelia Morata-Moreno, Rocio Alfaro-Ruiza, Agustin Lara-Sanchez, Jaime Esteban, Rafael Lujan, Carlos Alonso-Moreno, Pedro Segui, Alberto Ocana, Angel Lopez Gonzalez, John J. Aguilera-Correa, Francisco C. Perez-Martinez, Milagros Molina Alarcon
Summary: This study aimed to translate clinical research outcomes based on metallodrugs into clinical practice for the treatment of MRSA. The novel metallodrug 2P-Pt showed antibacterial effect against MRSA and inhibited biofilm formation. These findings suggest that 2P-Pt may be a promising therapeutic alternative for MRSA infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Teresa Fasciana, Maria Letizia Gargano, Nicola Serra, Elena Galia, Ignazio Arrigo, Maria Rita Tricoli, Orazia Diquattro, Giuseppa Graceffa, Salvatore Vieni, Giuseppe Venturella, Anna Giammanco
Summary: The study found that extracts from albino Grifola frondosa could effectively inhibit the growth of certain bacteria and biofilm production by Staphylococcus aureus, reducing the presence of biofilm. This indicates that albino Grifola frondosa extracts could be utilized as functional food and natural additives for food processing control and safety.
Article
Biotechnology & Applied Microbiology
Georgios Katsipis, Anastasia A. Pantazaki
Summary: In this study, the antimicrobial effects of SPT on Staphylococcus aureus biofilms were demonstrated. SPT reduced the content of cell wall components, altered the activity of alkaline phosphatases and proteins involved in phosphate metabolism, and weakened the resistance of bacteria to biofilm formation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Medicine, General & Internal
Ahmed Alsolami, Naif Saad ALGhasab, Mohammed S. M. Alharbi, Abdelhafiz I. Bashir, Mohd Saleem, Azharuddin Sajid Syed Khaja, Dakheel F. Aldakheel, Ehab Rakha, Jabar Aziz Alshammari, Taha E. Taha, Ziyad Melibari, Yaseer H. Alharbi, Ali A. Almutlag, Kamaleldin B. Said
Summary: MRSA lineages are a significant clinical and public health issue, and there is limited data on local lineage profiles. This study reports on the frequency of community-acquired and hospital-acquired MRSA cases. The majority of the isolates were community-acquired MRSA, with variations in infection types between males and females.
Article
Infectious Diseases
Stefan Monecke, Amira K. Bedewy, Elke Mueller, Sascha D. Braun, Celia Diezel, Amel Elsheredy, Ola Kader, Martin Reinicke, Abeer Ghazal, Shahinda Rezk, Ralf Ehricht
Summary: The study characterised MRSA isolates from a tertiary care centre in Alexandria, Egypt. The isolates belonged to 11 different clonal complexes and 14 strains, with CC15-MRSA-[V+fus] and CC1-MRSA-[V+fus+tir+ccrA/B-1] (PVL+) being the most common strains. Most isolates carried variant SCCmec elements and the fusidic acid resistance gene fusC. PVL genes were also prevalent. The study revealed an increase in fusC and PVL carriage and a decrease in the prevalence of the CC239 strain when compared to data from a previous study.
Article
Microbiology
Nicholas J. Pitcher, Andries Feder, Nicholas Bolden, Christian F. Zirbes, Anthony J. Pamatmat, Linda Boyken, Jared J. Hill, Alyssa R. Bartels, Andrew L. Thurman, Valerie C. Reeb, Harry S. Porterfield, Ahmed M. Moustafa, Paul J. Planet, Anthony J. Fischer
Summary: Linezolid, an antibiotic used to treat serious Staphylococcus aureus infections, can lead to the emergence of resistance in CF patients. In addition to a common mutation in the 23S ribosomal RNA, S. aureus strains can develop novel resistance through a combination of mutations affecting the bacterial ribosome. Linezolid resistance in CF patients is transient, possibly due to the growth disadvantage of resistant strains. However, chronic exposure to Linezolid may create optimal conditions for the future emergence of resistance.
MICROBIOLOGY SPECTRUM
(2023)
Review
Immunology
Alexander L. Lewis Marffy, Alex J. McCarthy
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Microbiology
Sian Marie Frosini, Ross Bond, Alex J. McCarthy, Claudia Feudi, Stefan Schwarz, Jodi A. Lindsay, Anette Loeffler
Article
Biochemistry & Molecular Biology
Nina M. van Sorge, Daniel A. Bonsor, Liwen Deng, Erik Lindahl, Verena Schmitt, Mykola Lyndin, Alexej Schmidt, Olof R. Nilsson, Jaime Brizuela, Elena Boero, Eric J. Sundberg, Jos A. G. van Strijp, Kelly S. Doran, Bernhard B. Singer, Gunnar Lindahl, Alex J. McCarthy
Summary: Streptococcus agalactiae, also known as group B Streptococcus (GBS), is a major cause of neonatal sepsis in humans. In a recent study, it was found that the surface-expressed beta protein of GBS binds to human CEACAM1 and CEACAM5 receptors, with the crystal structure revealing a novel IgI3 fold. This discovery suggests a potentially widely applicable mechanism for bacteria to target CEACAMs.
Article
Infectious Diseases
Valerie O. Baede, Michael Z. David, Arjana Tambic Andrasevic, Dominique S. Blanc, Michael Borg, Grainne Brennan, Boudewijn Catry, Aurelie Chabaud, Joanna Empel, Hege Enger, Marie Hallin, Marina Ivanova, Andreas Kronenberg, Kuntaman Kuntaman, Anders Rhod Larsen, Katrien Latour, Jodi A. Lindsay, Bruno Pichon, Dewi Santosaningsih, Leo M. Schouls, Francois Vandenesch, Guido Werner, Dorota Zabicka, Helena Zemlickova, Harald Seifert, Margreet C. Vos
Summary: This study examined 24 MRSA surveillance programs in 16 countries and found that the current programs rely on heterogeneous data collection systems, which hampers international epidemiological monitoring and research. To harmonize MRSA surveillance, the study suggests improving data integration, establishing central biobanks for MRSA isolates, and expanding surveillance to include skin and soft-tissue infection cases.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Multidisciplinary Sciences
Jesper Larsen, Claire L. Raisen, Xiaoliang Ba, Nicholas J. Sadgrove, Guillermo F. Padilla-Gonzalez, Monique S. J. Simmonds, Igor Loncaric, Heidrun Kerschner, Petra Apfalter, Rainer Hartl, Ariane Deplano, Stien Vandendriessche, Barbora Cerna Bolfikova, Pavel Hulva, Maiken C. Arendrup, Rasmus K. Hare, Celine Barnadas, Marc Stegger, Raphael N. Sieber, Robert L. Skov, Andreas Petersen, Oystein Angen, Sophie L. Rasmussen, Carmen Espinosa-Gongora, Frank M. Aarestrup, Laura J. Lindholm, Suvi M. Nykasenoja, Frederic Laurent, Karsten Becker, Birgit Walther, Corinna Kehrenberg, Christiane Cuny, Franziska Layer, Guido Werner, Wolfgang Witte, Ivonne Stamm, Paolo Moroni, Hannah J. Jorgensen, Herminia de Lencastre, Emilia Cercenado, Fernando Garcia-Garrote, Stefan Borjesson, Sara Haeggman, Vincent Perreten, Christopher J. Teale, Andrew S. Waller, Bruno Pichon, Martin D. Curran, Matthew J. Ellington, John J. Welch, Sharon J. Peacock, David J. Seilly, Fiona J. E. Morgan, Julian Parkhill, Nazreen F. Hadjirin, Jodi A. Lindsay, Matthew T. G. Holden, Giles F. Edwards, Geoffrey Foster, Gavin K. Paterson, Xavier Didelot, Mark A. Holmes, Ewan M. Harrison, Anders R. Larsen
Summary: This study reveals that methicillin-resistant Staphylococcus aureus (MRSA) strains were present in European hedgehogs before the discovery of antibiotics and have spread among hedgehog populations and between hedgehogs and secondary hosts. The study also shows that the hedgehog dermatophyte Trichophyton erinacei produces two beta-lactam antibiotics that give an advantage to MRSA strains. These findings emphasize the importance of understanding and managing antibiotic resistance in both wild animals and different ecosystems.
Article
Infectious Diseases
Anneke S. de Vos, Sake J. de Vlas, Jodi A. Lindsay, Mirjam E. E. Kretzschmar, Gwenan M. Knight
Summary: The study showed that higher prevalence may advantage a CC by allowing it to acquire antimicrobial resistances more easily. Due to density dependence in competition, dominance in an area can depend on historic contingencies; the MRSA CC that happened to be first could stay dominant because of its high prevalence advantage. This could help explain the stability of geographic differences in MRSA CC.
Article
Microbiology
Quentin J. Leclerc, Jacob Wildfire, Arya Gupta, Jodi A. Lindsay, Gwenan M. Knight
Summary: Research has shown that bacteriophages can lead to the evolution of multidrug-resistant bacteria through transduction in a short period of 8 hours, with the transmission of resistance genes occurring early. Mathematical models demonstrate that the dynamic interactions between phages and bacteria are crucial for the evolution of resistant strains.
Article
Biochemical Research Methods
Quentin J. Leclerc, Jodi A. Lindsay, Gwenan M. Knight
Summary: Depending on timing and concentration, phage and antibiotics can either work together to kill bacteria faster, or phage can generate multidrug-resistant bacteria by transduction which are then selected for by antibiotics.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Correction
Microbiology
Quentin J. Leclerc, Jacob Wildfire, Arya Gupta, Jodi A. Lindsay, Gwenan M. Knight
Article
Infectious Diseases
Valerie O. Baede, Arya Gupta, Gwenan M. Knight, Leo M. Schouls, Ken Laing, Mehri Tavakol, Anais Barray, Sake J. de Vlas, Anneke S. de Vos, Antoni P. A. Hendrickx, Madeeha Khan, Mirjam E. Kretzschmar, Willem J. B. van Wamel, Gerard Lina, Francois Vandenesch, Margreet C. Vos, Adam A. Witney, Jean-Philippe Rasigade, Jodi A. Lindsay
Summary: The MACOTRA consortium aimed to identify bacterial markers of epidemic success of MRSA isolates in Europe using a representative MRSA collection originating from France, the Netherlands and the United Kingdom. The results showed that MRSA antibiotic resistance profiles and antibiotic usage were associated with the incidence of infection and successful clonal spread, but there were variations among countries.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
J. B. Readman, M. Acman, A. Hamawandi, Cheng-Hsun Chiu, M. Sharland, J. A. Lindsay, J. F. Standing
Summary: In neonatal ICUs, ubiquitous infection with ESBL-producing Enterobacteriaceae is a major concern due to increasing antibiotic resistance. Distinguishing between bacterial and viral sepsis often leads to empirical antibiotics being used, contributing to resistance. Through testing, it was found that combining cefotaxime or ampicillin with sulbactam and gentamicin effectively inhibits the growth of ESBL-producing isolates and clears the infection. This combination showed consistent bactericidal activity at clinically achievable concentrations.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Erin A. Catton, Daniel A. Bonsor, Carolina Herrera, Margaretha Stalhammar-Carlemalm, Mykola Lyndin, Claire E. Turner, Jo Soden, Jos A. G. van Strijp, Bernhard B. Singer, Nina M. van Sorge, Gunnar Lindahl, Alex J. McCarthy
Summary: Infection with Streptococcus pyogenes after childbirth can have life-threatening consequences. Here, Catton et al. show that the epidemiologically associated bacterial R28 protein targets the human CEACAM1 receptor to facilitate the pathogenesis of bacterial sepsis.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Quentin Leclerc, Alastair Clements, Helen Dunn, James Hatcher, Jodi A. Lindsay, Louis Grandjean, Gwenan M. Knight
Summary: This study examined the antimicrobial resistance dynamics of Staphylococcus aureus in a UK pediatric hospital. It found that the changes in resistance were mainly driven by within-host evolution and between-host transmission. The study also highlighted the value of routine surveillance data in understanding antimicrobial resistance dynamics at both the individual and hospital levels.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Meeting Abstract
Pharmacology & Pharmacy
Madeeha Khan, Jodi Lindsay, Emma Baker
BRITISH JOURNAL OF PHARMACOLOGY
(2021)