Article
Agriculture, Dairy & Animal Science
Marta Leal, Catarina Morais, Barbara Ramos, Constanca Pomba, Patricia Abrantes, Sofia Santos Costa, Isabel Couto
Summary: This study aimed to evaluate the contribution of efflux to biocide and fluoroquinolone resistance in S. pseudintermedius. The results showed that efflux activity significantly increased resistance to biocides, which was strain-specific and glucose-dependent. Fluoroquinolone resistance was mainly related to target gene mutations. This study highlights the importance of efflux-mediated resistance in S. pseudintermedius and provides a methodological basis for further studies on this important veterinary pathogen.
Article
Infectious Diseases
Alessandro Bellato, Patrizia Robino, Maria Cristina Stella, Laura Scarrone, Daniela Scalas, Patrizia Nebbia
Summary: This study aimed to assess the resistance of Staphylococcus pseudintermedius isolates, against a broad panel of antibacterials, including exclusive to human medicine. The results showed that these strains had the highest resistance against veterinary medicine antibacterials, but there was also resistance against antibacterials exclusive to human medicine.
Article
Multidisciplinary Sciences
Nahathai Uttamamul, Manida Suksawat, Jutarop Phetcharaburanin, Supranee Jitpean, Aroonlug Lulitanond, Nattaya Sae-ung, Patcharee Boonsiri, Ratree Tavichakorntrakool
Summary: Staphylococcus pseudintermedius is a urease-producing bacteria that is a major cause of magnesium ammonium phosphate (MAP) urolithiasis in canine. Through metabolomics studies, we found that S. pseudintermedius undergoes metabolic changes after crystallization in artificial urine, which helps to explain the molecular mechanism behind the crystals induced by this bacteria strain.
Article
Infectious Diseases
Alice Wegener, Els M. Broens, Linda van der Graaf-van Bloois, Aldert L. Zomer, Caroline E. Visser, Jan van Zeijl, Coby van der Meer, Johannes G. Kusters, Alex W. Friedrich, Greetje A. Kampinga, Gregorius J. Sips, Leonard Smeets, Manfred E. J. van Kerckhoven, Arjen J. Timmerman, Jaap A. Wagenaar, Birgitta Duim
Summary: This study compared the genetic relatedness and antimicrobial resistance genes of canine and human S. pseudintermedius isolates. The high genetic diversity in MSSP isolates from both hosts and the importance of multidrug resistance were highlighted, with CC241 being a prevalent multidrug resistance genotype. No host-specific genes were significantly associated with either human or canine isolates in this genomic analysis.
Review
Veterinary Sciences
Stephanie A. Lynch, Karla J. Helbig
Summary: Staphylococcus pseudintermedius is a significant pathogenic bacterium in veterinary medicine, causing various infections in canines. The high prevalence of methicillin-resistant strains is concerning, prompting research into novel treatment avenues. Multiple new approaches, including vaccines and phage therapy, show promise in combatting MRSP infections in dogs. Further research is needed to address the challenges associated with MRSP.
VETERINARY SCIENCES
(2021)
Article
Multidisciplinary Sciences
K. Bierowiec, M. Miszczak, A. Korzeniowska-Kowal, A. Wzorek, D. Plokarz, A. Gamian
Summary: This study investigated the occurrence of Staphylococcus pseudintermedius in cats, revealing a prevalence of 2.49% in healthy cats and 7.61% in sick cats. Cats living with dogs were more likely to be colonized with the bacterium. Multidrug resistance was a common phenomenon among the isolates, highlighting the importance of monitoring antibiotic resistance in cats.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Elisa Rampacci, Tommaso Felicetti, Giada Cernicchi, Valentina Stefanetti, Stefano Sabatini, Fabrizio Passamonti
Summary: One approach to treating antibiotic-resistant bacteria is to co-administer efflux pump inhibitors (EPIs) with antibiotics to break resistances connected with antibacterial efflux. In this study, ten compounds were evaluated for their ability to inhibit efflux and synergize with different antibiotics in Staphylococcus pseudintermedius. Hit compounds 1, 6, and 8 were considered the best EPIs for S. pseudintermedius. These findings provide valuable data for further optimization and development of EPIs for treating staphylococcal infections.
Article
Microbiology
Bojan Papic, Majda Golob, Irena Zdovc, Darja Kusar, Jana Avbersek
Summary: Staphylococcus pseudintermedius is a common cause of skin infections in dogs, cats, and humans, with the frequency of MRSP strains increasing worldwide. A study in Slovenia found multiple MRSP transmission routes and highlighted the importance of MRSP-positive dogs in veterinary care-associated infections.
VETERINARY MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lopamudra Kher, Domenico Santoro, Karen Kelley, Daniel Gibson, Gregory Schultz
Summary: Nanosulfur exhibits antimicrobial and anti-biofilm activity against multidrug-resistant Staphylococcus pseudintermedius, while being ineffective against multidrug-resistant Pseudomonas aeruginosa. Nanosulfur has the potential to be used as an alternative to antimicrobials and anti-biofilm agents in veterinary and human medicine.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mariela E. Srednik, Claudia A. Perea, Gabriela I. Giacoboni, Jessica A. Hicks, Christine L. Foxx, Beth Harris, Linda K. Schlater
Summary: This study comparatively analyzed the population structure of Staphylococcus pseudintermedius associated with canine pyoderma in Argentina and the United States. The findings revealed that up to 62% of isolates exhibited multi-drug resistance. Several resistance genes and mutations were identified, indicating an emerging problem in antimicrobial resistance. Additionally, commonalities, such as the presence of sequence type ST71 in isolates from both countries, were observed. This study is important for monitoring the evolution and dissemination of antimicrobial resistance and gaining further insights into the development of this pathogen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Spencer A. Bruce, Joshua T. Smith, Jennifer L. Mydosh, John Ball, David B. Needle, Robert Gibson, Cheryl P. Andam
Summary: This study provides insights into the phylogenetic relationships and genetic resistance patterns of Staphylococcus pseudintermedius in New England and global contexts. Methicillin-resistant S. pseudintermedius with diverse resistance genes was found in the region, while the clonally expanding ST71 clone was not detected. The study highlights the importance of understanding the evolution and geographic spread of high-risk clones for the health of canine companions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Medicine, General & Internal
Sang Guen Kim, Sib Sankar Giri, Saekil Yun, Sang Wha Kim, Se Jin Han, Jun Kwon, Woo Teak Oh, Sung Bin Lee, Yong Ho Park, Se Chang Park
Summary: This study characterized antimicrobial resistance in Staphylococcus pseudintermedius from canine samples, and isolated two novel bacteriophages, pSp-J and pSp-S, from canine pet parks in South Korea with potential to control methicillin-resistant S. pseudintermedius. The phages showed stability and efficacy in preventing and degrading biofilms at different doses, demonstrating their potential for treating this pathogen.
FRONTIERS IN MEDICINE
(2021)
Article
Microbiology
Christiane Cuny, Franziska Layer-Nicolaou, Robert Weber, Robin Kock, Wolfgang Witte
Summary: This study investigated the nasal colonization of MSSA, MRSA, and Staphylococcus pseudintermedius in dogs and humans. The results showed that the risk of exchanging S. aureus/MRSA between humans and dogs is higher than that for S. pseudintermedius.
Article
Biology
Usasom Khongsri, Peerawit Chongrattanameteekul, Sineenart Chantarachart, Kornravee Photichai, Nittaya Chanayat, Thanya Varinrak, Raktham Mektrirat, Nuttawan Srifawattana
Summary: The prevalence of MRSP causing pyoderma is changing gradually, with annual variations. Research on cotrimazole susceptibility to MRSP is limited, but the empirical use of cotrimazole remains interesting. This study aimed to evaluate the susceptibility of cotrimazole to canine pyoderma MRSP isolates.
Article
Infectious Diseases
Kirill V. Ovchinnikov, Christian Kranjec, Tage Thorstensen, Harald Carlsen, Dzung B. Diep
Summary: The rise of antibiotic-resistant bacteria is a major challenge in human and veterinary medicine. This study highlights the importance of searching for antimicrobials for veterinary applications to prevent the misuse of frontline clinical antibiotics. The research shows that a combination of bacteriocins, namely micrococcin P1 and EntEJ97s, with conventional antibiotic penicillin G can synergistically combat methicillin-resistant Staphylococcus pseudintermedius (MRSP), a common pathogen in animal skin infections, and prevent the development of resistance. It also demonstrates the therapeutic potential of this combination in a murine skin infection model.
Article
Environmental Sciences
Dereje Tassew, Susan Fort, Yohannes Mebratu, Jacob McDonald, Hong Wei Chu, Hans Petersen, Yohannes Tesfaigzi
Summary: Exposure to wood smoke (WS) increases mucin expression more efficiently than exposure to cigarette smoke (CS), especially in a p53 pathway-dependent manner. The WS constituent oxalate can enhance mucin expression, particularly in individuals with a p53 arginine genotype, revealing potential mechanisms for increased risk of chronic bronchitis. Identifying responsible compounds and genetic variations could help estimate pollutant-related risks and improve lung disease management strategies.
ENVIRONMENTAL HEALTH PERSPECTIVES
(2022)
Review
Plant Sciences
Yuan Yee Lee, Sung Dae Kim, Seung-Chun Park, Man Hee Rhee
Summary: This article summarizes the therapeutic effects of ginseng on cardiovascular diseases, focusing on its influence on platelet aggregation, plaque formation, and thrombus formation.
JOURNAL OF GINSENG RESEARCH
(2022)
Review
Plant Sciences
Yuan Yee Lee, Yixian Quah, Jung-Hae Shin, Hyuk-Woo Kwon, Dong-Ha Lee, Jee Eun Han, Jin-Kyu Park, Sung Dae Kim, Dongmi Kwak, Seung-Chun Park, Man Hee Rhee
Summary: This article studies the potential use of Panax ginseng as a supplement to combat COVID-19 complications, especially thrombosis. The network pharmacology approach is used to validate the potential therapeutic effects of Panax ginseng on COVID-19-mediated thrombosis, the coagulation pathway, and platelet aggregation. The roles of Panax ginseng in platelet-leukocyte aggregates in relation to immunity-related responses in COVID-19 are also investigated.
JOURNAL OF GINSENG RESEARCH
(2022)
Article
Integrative & Complementary Medicine
Yuan Yee Lee, Heung Joo Yuk, Evelyn Saba, Sung Dae Kim, Dong-Seon Kim, Spandana Rajendra Kopalli, Jae-Wook Oh, Man Hee Rhee
Summary: The study demonstrates the potential of Duchesnea indica extract (DIE) to rescue mice from septic shock and sepsis by suppressing the expression of proinflammatory cytokines and preventing translocation of NF kappa B.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Immunology
Yuan Yee Lee, H. M. Arif Ullah, Lee Su Ha, Sung Dae Kim, Bong-Sik Yun, Man Hee Rhee
Summary: This study investigated the therapeutic efficacy of isopanepoxydone (ISO) isolated from Panus rudis against respiratory complications caused by particulate matter. The results showed that ISO effectively inhibited nitric oxide production, pro-inflammatory mediators, and cytokines in murine alveolar macrophages (MH-S cells). ISO also enhanced the expression of nuclear factor erythroid 2-related factor 2 and suppressed proteins in the inflammasome pathway, indicating its potential as a treatment for respiratory inflammation.
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
(2022)
Article
Pharmacology & Pharmacy
Muhammad Irfan, Tae-Hyung Kwon, Hyuk-Woo Kwon, Man Hee Rhee
Summary: Dieckol, a phlorotannin found in seaweeds with various properties, has been shown to have anti-platelet and anti-thrombotic effects. It inhibits platelet aggregation and secretion, regulates platelet adhesion and clot retraction, and activates the cAMP-PKA-VASP pathway. Animal experiments demonstrated its potential therapeutic use in treating and preventing platelet-related cardiovascular disorders.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Food Science & Technology
Hyuk-Woo Kwon, Muhammad Irfan, Yuan Yee Lee, Man Hee Rhee, Jung-Hae Shin
Summary: Cardiovascular diseases are a critical threat to our lives, and platelets play an important role in these diseases. This study evaluated the antiplatelet effects of artocarpesin, a compound isolated from Cudrania tricuspidata, and found that artocarpesin inhibits platelet activity and thrombus formation through the regulation of associated signaling molecules, highlighting its potential value in preventing platelet-induced cardiovascular diseases.
APPLIED BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Hae Soo Yun, Sylvatrie-Danne Dinzouna-Boutamba, Sanghyun Lee, Zin Moon, Dongmi Kwak, Dong-Il Chung, Yeonchul Hong, Man Hee Rhee, Youn-Kyoung Goo
Summary: The extract of Petasites japonicus shows potential antimalarial effects by inhibiting the proliferation of Plasmodium parasites and improving hematological parameters. These effects may be attributed to the inhibition of platelet activation related to attenuated glycoprotein VI downstream signaling.
Article
Integrative & Complementary Medicine
Yixian Quah, Jireh Chan Yi-Le, Na-Hye Park, Yuan Yee Lee, Eon-Bee Lee, Seung-Hee Jang, Min-Jeong Kim, Man Hee Rhee, Seung-Jin Lee, Seung-Chun Park
Summary: This study demonstrates the therapeutic benefits of Trifolium pratense ethanolic extract (TPEE) in preventing osteoporosis and maintaining the estrogen dependent characteristics of the uterus. The combination of recursive feature elimination with cross-validation (RFECV) and extreme gradient boosting (XGBoost) model can serve as a powerful tool for quick evaluation and diagnosis of postmenopausal osteoporosis.
Article
Plant Sciences
Yixian Quah, Yuan Yee Lee, Seung-Jin Lee, Sung Dae Kim, Man Hee Rhee, Seung-Chun Park
Summary: Hypercoagulability and platelet hyperactivity are common in severe COVID-19 patients. Natural remedies, such as Panax ginseng, are being explored for their potential in treating COVID-19 by targeting platelet activation and the coagulation cascade. Molecular docking analysis and pharmacokinetic calculations suggest that certain bioactive compounds in P. ginseng have favorable interactions with proteins and receptors involved in platelet activation and good pharmacokinetic profiles.
JOURNAL OF GINSENG RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Ji Sue Baik, You Na Seo, Young-Choon Lee, Joo Mi Yi, Man Hee Rhee, Moon-Taek Park, Sung Dae Kim
Summary: This study demonstrates that ionizing radiation activates the p38 MAPK-NLRC4-caspase-1 pathway in macrophages, leading to increased IL-1 beta production in response to lipopolysaccharide treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Hyuk-Woo Kwon, Man Hee Rhee, Jung-Hae Shin
Summary: In this study, it was found that the extract from the white-spotted flower chafer, Protaetia brevitarsis, can inhibit platelet aggregation by regulating cyclic nucleotides and signal transduction molecules. This suggests that the extract has strong antiplatelet effects and may be used to treat cardiovascular diseases associated with thrombosis and platelet activation.
PREVENTIVE NUTRITION AND FOOD SCIENCE
(2023)
Article
Microbiology
Chang Uk Chung, Haeseung Lee, Min-Goo Seo, Seung-Hun Lee, Kyoo-Tae Kim, Kaifa Nazim, Jung-Sun Song, Dong Hwa Bae, Man Hee Rhee, Oh-Deog Kwon, Dongmi Kwak
Summary: This study found that the overall infection rate of Theileria in Korean water deer was 8.1%, with 100% infection rates in the northern and southern regions. The central region had the lowest infection rate. Infection rates also varied depending on the season, with the highest rate observed in spring. Phylogenetic analysis revealed the presence of Theileria luwenshuni in the PCR-positive samples.
Article
Biochemistry & Molecular Biology
Hyuk-Woo Kwon, Sung Dae Kim, Man Hee Rhee, Jung-Hae Shin
Summary: Platelets play a significant role in hemostasis but can also contribute to the development of cardiovascular diseases. Controlling platelet function is important in preventing thrombosis. A compound called 5-hydroxyindolin-2-one (5-HI) has been found to inhibit human platelet aggregation without cytotoxicity and suppress intracellular Ca2+ concentration. It also regulates various pathways to inhibit thromboxane A(2) production and alpha IIb beta 3 action, suggesting its potential as a substance for preventing thrombosis-mediated thrombosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shin-Sook Yoon, Hyuk-Woo Kwon, Jung-Hae Shin, Man Hee Rhee, Chang-Eun Park, Dong-Ha Lee
Summary: This study investigates the effects and mechanisms of artesunate on platelet aggregation and thrombus formation. Results show that artesunate significantly increases cAMP and cGMP production, inhibits calcium ion mobilization, and reduces platelet activity and fibrinogen binding. It also decreases the production of TXA(2) and intracellular granular secretion. These findings suggest that artesunate has potential as an antiplatelet substance to inhibit platelet aggregation and thrombus formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)