Article
Biochemistry & Molecular Biology
Jun Wan, Hongji Dai, Xiaoli Zhang, Sheng Liu, Yuan Lin, Ally-Khan Somani, Jingwu Xie, Jiali Han
Summary: The majority of non-melanoma skin cancer cases are basal cell carcinoma and squamous cell carcinoma which originate from keratinocytes. The incidence of keratinocyte carcinomas in the US is over 5 million per year, three times higher than all other types of cancer combined. Research has shown significant variations in gene expression in these tumors, with novel findings including up-regulation of zinc finger encoding genes in human BCC and a novel link between hedgehog signaling, Wnt signaling, and the cilium.
Article
Cell Biology
Zhiran Zou, Xiao Long, Qian Zhao, Yandong Zheng, Moshi Song, Shuai Ma, Yaobin Jing, Si Wang, Yifang He, Concepcion Rodriguez Esteban, Nanze Yu, Jiuzuo Huang, Piu Chan, Ting Chen, Juan Carlos Izpisua Belmonte, Weiqi Zhang, Jing Qu, Guang-Hui Liu
Summary: Single-cell RNA sequencing of human eyelid skin revealed progressive accumulation of photoaging-related changes and increased chronic inflammation with age. Transcriptional factors involved in the developmental process showed early-onset decline during aging. Inhibition of key transcription factors HES1 and KLF6 compromised cell proliferation, increased inflammation, and accelerated cellular senescence during aging.
DEVELOPMENTAL CELL
(2021)
Editorial Material
Oncology
Conor E. Steuer, Nabil F. Saba
Summary: Recent evidence confirms the clinical benefit of PD-1 inhibitors in advanced cutaneous squamous cell carcinomas. The activity of different agents appears to be equivalent. The replication of these findings raises the question of substituting immunotherapeutic agents based on smaller trials.
Article
Biochemistry & Molecular Biology
Snezana Hinic, April Rich, Nicole Anayannis, Stephanie Cabarcas-Petroski, Laura Schramm, Patricio Meneses
Summary: High-risk human papillomaviruses (HPV) play a significant role in the development of head and neck squamous cell carcinomas (HNSCC), and are associated with differential gene expression and DNA methylation patterns. The study identified differential gene expression, differential methylation, and altered pathways between HPV positive and HPV negative HNSCC groups, highlighting genes such as SYCP2 and TAF7L that are implicated in HPV positive HNSCC tumorigenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dermatology
Kylee J. B. Kus, Fadi Murad, Timothy D. Smile, Michael Chang, Sepideh Ashrafzadeh, Guohai Zhou, Evelyn O. Ilori, Shlomo A. Koyfman, Allison T. Vidimos, Chrysalyne D. Schmults, Emily S. Ruiz
Summary: Lymphovascular invasion (LVI) has a significant impact on tumor outcomes in cutaneous squamous cell carcinoma, with LVI-positive tumors showing higher rates of metastasis and death within 5 years.
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2022)
Article
Oncology
Tenny Mudianto, Katie M. Campbell, Jason Webb, Paul Zolkind, Zachary L. Skidmore, Rachel Riley, Erica K. Barnell, Ibrahim Ozgenc, Tusar Giri, Gavin P. Dunn, Douglas R. Adkins, Malachi Griffith, Ann Marie Egloff, Obi L. Griffith, Ravindra Uppaluri
Summary: The study revealed a Yap1-dependent resistance to trametinib therapy in HNSCCs, with downstream effectors of the Hippo pathway being activated. Combined targeting of Yap1 and MEK may enhance the therapeutic response in head and neck squamous cell carcinoma. The findings suggest potential strategies to overcome resistance and improve treatment outcomes.
CLINICAL CANCER RESEARCH
(2021)
Article
Oncology
Antonio Ghidini, Domenico Santangelo, Giovanni Vaccaro, Mario Chillura, Fausto Petrelli
Summary: This case report presents an elderly woman with locally advanced squamous carcinoma who showed significant response and good tolerability after receiving Cemiplimab therapy. It demonstrates that immunotherapy is a new treatment option for cSCC.
Article
Multidisciplinary Sciences
Peter Bailey, Rachel A. Ridgway, Patrizia Cammareri, Mairi Treanor-Taylor, Ulla-Maja Bailey, Christina Schoenherr, Max Bone, Daniel Schreyer, Karin Purdie, Jason Thomson, William Rickaby, Rene Jackstadt, Andrew D. Campbell, Emmanouil Dimonitsas, Alexander J. Stratigos, Sarah T. Arron, Jun Wang, Karen Blyth, Charlotte M. Proby, Catherine A. Harwood, Owen J. Sansom, Irene M. Leigh, Gareth J. Inman
Summary: By performing RNAseq transcriptomic profiling of 110 patient samples, we revealed a disease continuum from normal sun-exposed skin to precancerous actinic keratosis (AK), and then to malignant invasive cutaneous squamous cell carcinoma (cSCC) and potentially lethal metastatic disease. This progression is accompanied by the suppression of master regulators of epidermal differentiation, dynamic modulation of the epidermal differentiation complex, remodelling of the immune landscape, and an increase in tumor specific keratinocytes. Our study provides a comprehensive map of the cSCC disease continuum and reveals potentially actionable events that promote and accompany disease progression.
NATURE COMMUNICATIONS
(2023)
Review
Oncology
Miguel Alonso-Juarranz, Marta Mascaraque, Elisa Carrasco, Tamara Gracia-Cazana, Oscar De La Sen, Yolanda Gilaberte, Salvador Gonzalez, Angeles Juarranz, Farzin Falahat
Summary: In this review, recent studies on the genetic alterations and stroma composition of oral and cutaneous squamous cell carcinomas (OSCC and CSCC) were described. Mutations in tumor suppressor genes and protooncogenes contribute to the differentiation, aggressiveness, and metastatic potential of these cancers. OSCC tumors exhibit a higher frequency of driver mutations in tumor suppressor genes compared to CSCC. Differences in tumor microenvironment composition influence the aggressiveness and outcomes of each tumor type. High levels of tumor-infiltrating lymphocytes are more frequently associated with positive outcomes in OSCC than CSCC.
Article
Pathology
Paul W. Harms, Monique E. Verhaegen, Kevin Hu, Steven M. Hrycaj, May P. Chan, Chia-Jen Liu, Marina Grachtchouk, Rajiv M. Patel, Aaron M. Udager, Andrzej A. Dlugosz
Summary: MCC and SCCIS show significant overlap in gene mutations, with no unique gene mutations found in all cases of MCC. Transcriptome analysis reveals large differences in gene expression between MCC and SCCIS, including epidermal markers and immune genes. Immunohistochemistry studies show increased expression of SOX2 in the MCC component.
Article
Veterinary Sciences
B. L. Sanz Ressel, A. R. Massone, C. G. Barbeito
Summary: The study found that the coexpression of p63 and CK5 in canine cutaneous tumors may indicate epidermal keratinocytes with self-renewal capacity, suggesting that transit amplifying cells and even differentiated keratinocytes may also contribute to the pathogenesis of epidermal tumors in dogs.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Oncology
Matthew L. Fisher, Seamus Balinth, Yon Hwangbo, Caizhi Wu, Carlos Ballon, John E. Wilkinson, Gary L. Goldberg, Alea A. Mills
Summary: BRD4 plays a critical role in the regulation of cancer stem-like properties and is highly elevated in the aggressive subpopulation of cells in squamous cell carcinomas. Targeting BRD4 can effectively inhibit Delta Np63 alpha, reducing spheroid formation and inhibiting tumor growth.
Article
Biochemistry & Molecular Biology
Leonor Delgado, Paula Brilhante-Simoes, Fernanda Garcez, Luis Monteiro, Isabel Pires, Justina Prada
Summary: p-mTOR and p-S6 proteins are commonly expressed in canine oral squamous cell carcinoma (OSCC), and p-S6 expression is correlated with poor cancer-specific survival (CSS) in dogs with OSCC.
Article
Oncology
Glenn Geidel, Isabel Heidrich, Julian Koett, Stefan W. Schneider, Klaus Pantel, Christoffer Gebhardt
Summary: This article provides an overview of the current diagnostic and therapeutic standards for advanced cutaneous squamous cell carcinoma (cSCC), and discusses the emerging diagnostic approaches such as liquid biopsy and tumor tissue-based analyses.
NPJ PRECISION ONCOLOGY
(2022)
Article
Veterinary Sciences
Ryo Miyamoto, Hiroyuki Tani, Tomoyo Ikeda, Hono Saima, Kyoichi Tamura, Makoto Bonkobara
Summary: The study found that the survivin inhibitor YM155 can inhibit the growth of canine squamous cell carcinoma cells by activating autophagy, leading to cell death.
RESEARCH IN VETERINARY SCIENCE
(2021)
Letter
Veterinary Sciences
Alessio Arbuatti, Sabrina Vanessa Patrizia Defourny, Leonardo Della Salda, Mariarita Romanucci
JOURNAL OF EXOTIC PET MEDICINE
(2021)
Letter
Veterinary Sciences
Mariarita Romanucci, Giuseppe Borzacchiello, Sabrina Vanessa Patrizia Defourny, Aldo Corvini, Gennaro Altamura, Lucio Petrizzi, Leonardo Della Salda
JOURNAL OF EXOTIC PET MEDICINE
(2020)
Article
Veterinary Sciences
Annalisa Rizzo, Leonardo Della Salda, Mariarita Romanucci, Stefano Ciccarelli, Carmela Valastro, Michela Galgano, Raffaele L. Sciorsci
JOURNAL OF THE SOUTH AFRICAN VETERINARY ASSOCIATION
(2020)
Article
Veterinary Sciences
Mariarita Romanucci, Paolo E. Crisi, Maria Veronica Giordano, Morena Di Tommaso, Francesco Simeoni, Leonardo Della Salda
Summary: A 14-year-old spayed female Labrador Retriever presented with chronic vomiting, diagnosed with gastric sarcoma which required partial gastrectomy. Pathological examination revealed features of gastric sarcoma, as well as Menetrier-like disease.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2021)
Review
Veterinary Sciences
Marcella Massimini, Mariarita Romanucci, Raffaella De Maria, Leonardo Della Salda
Summary: Canine tumors serve as valuable models for researching novel biomarkers and metastasis pathways; vasculogenic mimicry, a unique property of malignant cancer cells, offers opportunities for investigating molecular mechanisms and therapeutic targets related to metastasis.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Zoology
Pietro Giorgio Tiscar, Fernando Rubino, Barbara Paoletti, Cristina E. Di Francesco, Francesco Mosca, Leonardo Della Salda, Jasmine Hattab, Camilla Smoglica, Simone Morelli, Giovanni Fanelli
Summary: Since early autumn 2016, Mass Mortality Events (MME) have greatly affected the population of the fan mussel Pinna nobilis in the Mediterranean Sea. A newly described Haplosporidian species, Haplosporidium pinnae, is believed to be the causative agent of the mortality outbreak, along with opportunistic bacterial pathogens. In this study, we provided a cytological description of H. pinnae in moribund specimens of P. nobilis collected in the Gulf of Taranto. Molecular analysis also revealed the presence of bacteria from the Mycobacterium ulcerans-M. marinum complex.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Romina Fusillo, Mariarita Romanucci, Manlio Marcelli, Marcella Massimini, Leonardo Della Salda
Summary: The study of post-mortem examination of Eurasian otters in Italy revealed that vehicle collision was the main cause of death, especially in young individuals, and measures need to be taken to reduce the risk. Most otters were in good nutritional condition and between 1 and 3 years old. Necropsy examinations showed different signs of disease in some individuals, but ticks and endoparasites were rarely detected.
Article
Agriculture, Dairy & Animal Science
Ilaria Falerno, Roberto Tamburro, Francesco Collivignarelli, Leonardo Della Salda, Luigi Navas, Rossella Terragni, Paolo Emidio Crisi, Andrea Paolini, Francesco Simeoni, Massimo Vignoli
Summary: This study evaluates the feasibility and diagnostic yield of pulmonary cryobiopsy compared to surgical lung biopsy (SLB) in dogs. The results show that cryobiopsy samples are smaller than SLB samples but still provide sufficient tissue for diagnosis. Cryobiopsy samples have fewer artifacts and a higher percentage of alveolar tissue compared to SLB samples. Therefore, pulmonary cryobiopsy is a feasible and useful alternative to SLB for lung histopathological examination in dogs.
Article
Veterinary Sciences
Francesca Del Signore, Massimo Vignoli, Leonardo Della Salda, Roberto Tamburro, Andrea Paolini, Ilaria Cerasoli, Matteo Chincarini, Emanuela Rossi, Nicola Ferri, Mariarita Romanucci, Ilaria Falerno, Francesco de Pasquale
Summary: In this study, a T1 and T2-based relaxometry approach was developed to characterize subtle brain lesions obtained with autologous blood injections in rabbits. The approach involved a semi-automatic hierarchical classification of different regions based on their relaxometry properties. The study utilized a low-field scanner commonly found in veterinary clinics. The proposed classifier demonstrated moderate accuracy in lesion characterization.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Francesca Del Signore, Germain Arribarat, Leonardo Della Salda, Giovanni Mogicato, Alexandra Deviers, Benjamin Cartiaux, Massimo Vignoli, Patrice Peran, Francesco de Pasquale
Summary: This study assessed the segmentation variability of Beagle dog brain MRI and the effect of formalin fixation. The segmentation was found to be reproducible, and formalin fixation improved brain structure visualization and segmentation.
Editorial Material
Veterinary Sciences
Mariarita Romanucci, Raffaella De Maria, Emanuela Maria Morello, Leonardo Della Salda
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Matteo Oliveri, Cristina Carnabuci, Massimo Vignoli, Simone DI Feliciantonio, Marco DI Feliciantonio, Leonardo DELLA Salda, Zdenek Knotek, Morena DI Tommaso, Alessia Luciani
Summary: The study aimed to describe the important cardiac structures in Heterodon nasicus using echocardiography and anatomical dissection. Echocardiographic measurements were performed on healthy adult Heterodon nasicus, and the measurements of ventricular length, aortic diameter, pulmonary trunk diameter, and thicknesses of the interventricular septum and wall of the cavum pulmonale and cavum arteriosum were recorded. Aortic flow and pulmonary trunk flow were also measured. Dissection of two specimens was done. The study found differences in pulmonary trunk diameter between male and female Heterodon nasicus, and a moderate interventricular right to left shunt in all specimens.
VETERINARNI MEDICINA
(2023)
Article
Dermatology
Leonardo Della Salda, Laura Bongiovanni, Marcella Massimini, Mariarita Romanucci, Antonella Vercelli, Alessia Colosimo, Ramona Di Matteo, Sabrina Vanessa Patrizia Defourny
Summary: Through immunohistochemical staining of skin biopsies from Pomeranians affected by alopecia X, it was found that there was a significant decrease in the immunexpression of p63 in the hair follicles, indicating the involvement of p63 in the hair cycle.
VETERINARY DERMATOLOGY
(2023)
Article
Veterinary Sciences
Martina Rosto, Sara Canal, Leonardo Della Salda, Carlo Cantile, Alberto Cauduro, Rossella Terragni, Andrea De Bonis, Francesca Del Signore, Massimo Vignoli
Summary: This article presents a clinical case of brain cyst in a Belgian Malinois dog. The study suggests that the clinical symptoms related to cysts could be associated with the size of the cyst and the impact on surrounding neuroanatomical structures.
ACTA VETERINARIA-BEOGRAD
(2023)
Article
Veterinary Sciences
Marcella Massimini, Alessia Gloria, Mariarita Romanucci, Leonardo Della Salda, Lucia Di Francesco, Alberto Contri
Summary: This study compared two different sonoelastography methods in evaluating canine mammary lesions. The results showed that shear-wave elastography was more replicable compared to strain elastography, and both techniques were correlated with the amount of connective tissue in the lesions. However, both techniques were unable to distinguish between benign and malignant mammary tumors due to the wide variable content in the connective tissue. Therefore, sonoelastography can be useful in characterizing connective tissue content in canine mammary tumors but should be used in conjunction with other techniques to evaluate the malignancy of the lesions.
VETERINARY SCIENCES
(2022)