Article
Public, Environmental & Occupational Health
Rody Blom, Maarten J. J. Schrama, Jeroen Spitzen, Babette F. M. Weller, Anne van der Linden, Reina S. Sikkema, Marion P. G. Koopmans, Constantianus J. M. Koenraadt
Summary: Genomic analyses of mosquito-borne virus outbreaks in temperate Europe have shown the presence of similar virus strains over multiple years, indicating local overwintering. It is unclear how these viruses persist in winter, but diapausing mosquitoes may play a role in their persistence.
Article
Virology
Stephanie Jansen, Renke Luehken, Michelle Helms, Bjoern Pluskota, Wolf Peter Pfitzner, Sandra Oerther, Norbert Becker, Jonas Schmidt-Chanasit, Anna Heitmann
Summary: Transmission of arboviruses, such as the Sindbis virus, by mosquitoes is a global health threat. This study found that all investigated Culex and Aedes mosquito species have the ability to transmit Sindbis virus, independent of temperature.
Article
Cell Biology
Jialu Qiao, Qingzhen Liu
Summary: The study revealed the interaction between autophagy and Sindbis virus replication in Aedes mosquito cells, showing that autophagy manipulation plays a role in viral replication and could potentially help in controlling mosquito-borne diseases. The differing patterns of autophagy induction and suppression in C6/36 and Aag2 cells during SINV replication suggest the complexity of viral-vector interactions. Understanding these interactions could aid in elucidating the differences in viral transmission capacity between Ae. albopictus and Ae. aegypti.
CELLULAR SIGNALLING
(2022)
Article
Virology
Nazli Ayhan, Aissam Hachid, Laurence Thirion, Kamel Eddine Benallal, Laura Pezzi, Fayez Ahmed Khardine, Chahrazed Benbetka, Sihem Benbetka, Zoubir Harrat, Remi Charrel
Summary: Sindbis virus (SINV), a zoonotic alphavirus, has been identified as a cause of human diseases in Africa, Europe, Asia, and Australia. In this study, SINV was detected and isolated from mosquitoes in Algeria, and genetic analysis revealed its similarity to a strain found in Kenya.
Article
Multidisciplinary Sciences
Hui-Chung Lin, Der-Jiang Chiao, Chang-Chi Lin, Szu-Cheng Kuo
Summary: The study demonstrates efficient delivery of CHIKV replicons into cells using a baculovirus, verifying its efficacy through experiments, offering insights into virus replication mechanisms and potential for antiviral development.
SCIENTIFIC REPORTS
(2021)
Article
Parasitology
Lincoln Timinao, Rebecca Vinit, Michelle Katusele, Louis Schofield, Thomas R. Burkot, Stephan Karl
Summary: Various parameters prior to and during direct membrane feeding on Anopheles farauti sensu stricto were evaluated for their impact on feeding rates. Optimal conditions involved offering 50 female mosquitoes in a cup, using overnight starved mosquitoes, providing 350-500 μL of blood via a Baudruche membrane, for at least 10-20 minutes.
PARASITES & VECTORS
(2021)
Article
Multidisciplinary Sciences
Fei Wang, Cihan Yang, Shunlong Wang, Qun Wu, Christabel Ochieng, Zhiming Yuan, Han Xia
Summary: Mosquito-borne viruses pose a severe threat to public health as they are transmitted by many mosquito species. This study presents a method for detecting viruses in the salivary glands of Aedes aegypti, which can help assess the risk of these viruses spread by mosquitoes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Virology
Alexis Carpenter, Scott R. Santos, Rollie J. Clem
Summary: Avoiding apoptosis has been shown to be a determinant of successful arbovirus infection of arthropod vectors. Expressing Reaper by inserting the Reaper gene can significantly reduce infection prevalence in Aedes aegypti mosquitoes, but its ability to continue suppressing virus replication is limited once infection is established.
Article
Multidisciplinary Sciences
Xiaonan Dong, Yuting Yang, Zhongju Zou, Yuting Zhao, Bo Ci, Lin Zhong, Madhura Bhave, Liwei Wang, Yi-Chun Kuo, Xiao Zang, Rui Zhong, Elizabeth R. Aguilera, R. Blake Richardson, Boris Simonetti, John W. Schoggins, Julie K. Pfeiffer, Li Yu, Xuewu Zhang, Yang Xie, Sandra L. Schmid, Guanghua Xiao, Paul A. Gleeson, Nicholas T. Ktistakis, Peter J. Cullen, Ramnik J. Xavier, Beth Levine
Summary: Autophagy plays a crucial role in immunity, especially in initiating autophagy during viral infection. SNX5 activates the PI3KC3 complex on endosomes to promote the initiation of autophagy, which is important for cellular defense against viral infections.
Article
Virology
Peter Hodoameda, Linus Addae, Rollie J. Clem
Summary: The mechanisms involved in determining arbovirus vector competence are still not fully understood. Genetic differences between mosquito populations are known to contribute to the variability in vector competence. This study found that the midgut barrier and the length of the incubation period can affect the variability in virus titer between individual mosquitoes. Other factors such as virus and mosquito strain, mosquito age and weight, blood ingested, and virus concentration in the blood meal had no discernible effect on titer variability. Differences were also observed in culture adaptability and oral infection ability between virus populations obtained from mosquitoes with low and high titers.
Article
Parasitology
Arturo Hernandez-Colina, Merit Gonzalez-Olvera, Emily Lomax, Freya Townsend, Amber Maddox, Jenny C. Hesson, Kenneth Sherlock, Dawn Ward, Lindsay Eckley, Mark Vercoe, Javier Lopez, Matthew Baylis
Summary: The research on mosquito feeding behavior in zoos revealed that mosquitoes mainly feed on birds, non-human mammals, and humans, with feeding proportions varying across seasons and sites. The study highlighted the importance of monitoring mosquito activity in complex settings for planning effective control strategies and reducing disease transmission risks for humans and zoo vertebrates.
PARASITES & VECTORS
(2021)
Article
Parasitology
Tilahun Adugna, Delensaw Yewhelew, Emana Getu
Summary: The research in Bure district, Ethiopia found that Anopheles mosquitoes had mixed blood feeds, with a preference for human blood for egg development. This suggests the need to strengthen zooprophylaxis as a means of vector control to reduce malaria transmission.
PARASITES & VECTORS
(2021)
Article
Virology
Feixiang Yan, Wei Yang, Xinlu Wang, Guangxia Gao
Summary: This study found that the ISG product TMEM45B inhibits the replication of Sindbis virus (SINV). TMEM45B interacts with the viral nonstructural proteins Nsp1 and Nsp4, inhibiting the translation and promoting the degradation of SINV RNA. In addition, TMEM45B also interacts with Nsp1 and Nsp4 of chikungunya virus (CHIKV), suggesting its potential role in inhibiting the replication of other alphaviruses.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Andargie Abate, Soriya Kedir, Mitiku Bose, Jifar Hassen, Laurent Dembele, Lemu Golassa
Summary: The membrane feeding assay was used to determine the infectivity of blood meals from symptomatic Plasmodium-infected patients to mosquitoes, revealing high infectiousness of symptomatic patients and confirming human as an infectious reservoir of malaria.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Seline Omondi, Jackline Kosgei, Silas Agumba, Brian Polo, Nick Yalla, Vincent Moshi, Bernard Abong'o, Maurice Ombok, Daniel P. P. McDermott, Julian Entwistle, Aaron M. M. Samuels, Feiko O. O. Ter Kuile, John E. E. Gimnig, Eric Ochomo
Summary: This study evaluated the sugar-feeding behavior of Anopheles mosquitoes in western Kenya and found that they can obtain nutrients from natural sugar sources. These findings offer a potential avenue for controlling mosquitoes through the use of attractive targeted sugar baits (ATSBs).
SCIENTIFIC REPORTS
(2022)