A novel isochroman derivative inhibited apoptosis in vascular endothelial cells through depressing the levels of integrin β4, p53 and ROS

A new derivative of isochroman, 7-(isopropoxymethyl)-5-phenyl-7,8-dihydro-5H-[1,3]dioxolo[4,5-g]isochromene (ISO-9), was synthesized in our laboratory. In this study, we investigated the effect of ISO-9 on the apoptosis induced by deprivation of serum and fibroblast growth factor-2 (FGF-2) in human umbilical vein vascular endothelial cells (HUVECs). The results of MTT assay showed that 40 mu M ISO-9 prevented the reduction of cell viability induced by the deprivation of serum and FGF-2 at 24 h or 48 h, respectively. To further study the correlated mechanism, the levels of integrin beta 4, p53 and reactive oxygen species (ROS) were analyzed. The results showed that the high levels of integrin beta 4, p53 and ROS induced by the deprivation of serum and FGF-2 could be inhibited by the treatment of 40 mu M ISO-9. The data suggested that ISO-9 was a promising antiapoptotic agent and could be served as a useful tool to study the molecular mechanism of apoptosis in vascular endothelial cells (VECs). (C) 2008 Elsevier Inc. All rights reserved.