Article
Immunology
Sirikorn Kitiyodom, Teerapong Yata, Kim D. Thompson, Janina Costa, Preetham Elumalai, Takayuki Katagiri, Sasithon Temisak, Katawut Namdee, Channarong Rodkhum, Nopadon Pirarat
Summary: The study confirmed that the chitosan-based mucoadhesive nanovaccine is an effective platform for immersion vaccination of tilapia, with vaccinated fish generating a humoral systemic immune response against columnaris disease. Fish mortality rate significantly decreased in the vaccinated group compared to the unvaccinated control group.
Article
Immunology
Wenlong Cai, Covadonga R. Arias
Summary: A modified-live vaccine against columnaris disease in catfish aquaculture was developed by selecting mutants resistant to rifampin. Comparative genomic analysis revealed 16 unique single nucleotide polymorphisms in the attenuated mutant strain, affecting RNA polymerase activity and potentially disrupting protein secretion systems.
Article
Biotechnology & Applied Microbiology
Benjamin R. LaFrentz, Stanislava Kralova, Claire R. Burbick, Trevor L. Alexander, Conner W. Phillips, Matt J. Griffin, Geoffrey C. Waldbieser, Julio C. Garcia, Fernanda de Alexandre Sebastiao, Esteban Soto, Thomas P. Loch, Mark R. Liles, Kevin R. Snekvik
Summary: This study determined the taxonomic status of four genetic groups of Flavobacterium columnare using polyphasic and phylogenomic approaches. Phylogenetic analyses demonstrated that the four genetic groups formed well supported and distinct clades within the genus Flavobacterium. Biochemical and physiological characteristics were similar among the groups, but quantitative differences in fatty acid profiles were detected and MALDI-TOF analyses showed unique peaks for each group. Therefore, the names F. covae sp. nov., F. davisii sp. nov., and F. oreochromis sp. nov. are proposed for genetic groups 2, 3, and 4, respectively.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2022)
Article
Fisheries
Zijun Lu, Ren Gao, Yafei Duan, Rui Han, Wenjie Guo, Xueming Dan, Yanwei Li
Summary: This study isolated 45 bacterial strains of F. columnare from grass carp in various provinces of China, identifying three genotypes with genomovar I being the dominant type. Analysis revealed genetic differences among the three genomovars, with a closer relationship between genomovar I and II. The study provides insight into the genetic diversity of F. columnare in Chinese grass carp.
Article
Fisheries
Nantawut Ponpukdee, Pradit Wangman, Channarong Rodkhum, Chalinan Pengsuk, Parin Chaivisuthangkura, Paisarn Sithigorngul, Siwaporn Longyant
Summary: Monoclonal antibodies generated from outer membrane proteins of pathogenic Flavobacterium columnare can be used as a simple, fast, specific, and low-cost immunological tool for the direct detection of F. columnare without the need for bacterial isolation as in conventional biochemical tests or DNA extraction as in PCR assays. By combining specific MAbs, the detection limit in dot blotting assay can be increased to 5 x 10(2) cfu/dot.
Article
Biotechnology & Applied Microbiology
Rachel A. Conrad, Jason P. Evenhuis, Ryan S. Lipscomb, Clayton Birkett, Mark J. McBride
Summary: Columnaris disease caused by Flavobacterium columnare is a major challenge for freshwater aquaculture. The virulence factors and control methods of F. columnare are poorly understood. This study revealed that iron acquisition mechanisms, such as siderophores, are not critical virulence factors for F. columnare.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Fisheries
Shivam Singh, Sumanta Kumar Mallik, Krishna Kala, Neetu Shahi, Richa Pathak, Abhay Kumar Giri, Suresh Chandra, Kushagra Pant, Rabindar Singh Patiyal
Summary: The study identified Flavobacterium columnare as the causative agent in columnaris disease in farmed rainbow trout in Central Indian Himalayan region. It confirmed the high virulence of the F. columnare BRTAGILLFC 01 strain through in-vitro assays and experimental infection in rainbow trout fingerlings. The study also revealed the susceptibility of the bacterium to certain antibiotics at lower concentrations, demonstrating the potential for antibiotic treatment in disease control.
Article
Marine & Freshwater Biology
Mahsa Hajisafarali, Sari Aaltonen, Katja Pulkkinen, Jouni Taskinen
Summary: The global decline of freshwater mussels poses a threat to biodiversity and ecosystem services. This study found that freshwater mussels can effectively reduce bacterial fish pathogens in water, but did not significantly decrease algal cell concentration. These findings suggest that freshwater mussels may have the potential to aid in water quality management and aquaculture pathogen issues.
Article
Biotechnology & Applied Microbiology
Nicole C. Thunes, Rachel A. Conrad, Haitham H. Mohammed, Yongtao Zhu, Paul Barbier, Jason P. Evenhuis, David Perez-Pascual, Jean-Marc Ghigo, Ryan S. Lipscomb, John R. Schneider, Nan Li, Devon H. Erbes, Clayton Birkett, Benjamin R. LaFrentz, Timothy J. Welch, Mark J. McBride
Summary: Flavobacterium columnare, a pathogen that causes columnaris disease in freshwater fish, poses a major threat to sustainable aquaculture. Researchers have identified a genetically manipulable strain, MS-FC-4, which has facilitated the identification of virulence factors. Deletion of core genes eliminates virulence, and several secreted proteins have been identified as important for pathogenicity.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Heidi M. T. Kunttu, Anniina Runtuvuori-Salmela, Mathias Middelboe, Jason Clark, Lotta-Riina Sundberg
Summary: Bacteriophages can effectively prevent and treat bacterial infections in aquaculture settings, with bath treatments being the most efficient method for preventing infections. The presence of phage-coated plastic sheets in a flow-through system delayed the onset of disease, while oral administration of phages initially increased disease progression but led to lower total mortality. Successful phage therapy treatment in aquaculture requires optimization of in vivo phage delivery methods.
Article
Immunology
Wenjie Guo, Rui Han, Weizhen Xu, Zijun Lu, Yanwei Li, Xueming Dan, Zequan Mo
Summary: This study investigated the immune protective effects of an inactivated F. columnare vaccine. The results showed that when white oil adjuvant was used as a booster, the relative percent survival (RPS) after 8 weeks of vaccination was 34% and 61%, respectively. Furthermore, the inactivated vaccine comprising an appropriate antigen isolate with white oil adjuvant as a booster can provide effective protection in grass carp.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Infectious Diseases
Charles M. Gieseker, Patricia S. Gaunt, John P. Hawke, Tina C. Crosby, Nicholas R. Hasbrouck, Dana X. Gao, Annelies M. Declercq
Summary: Flavobacterium columnare, the causative agent of columnaris disease in freshwater fish, is a major problem in aquaculture. With limited antimicrobial therapies available, effective monitoring for changes in susceptibility is crucial. Researchers have conducted tests on multiple isolates and proposed interpretive criteria to aid in categorizing and monitoring antimicrobial susceptibility of this pathogen.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Fisheries
Sirikorn Kitiyodom, Clara Trullas, Channarong Rodkhum, Kim D. Thompson, Takayuki Katagiri, Sasithon Temisak, Katawut Namdee, Teerapong Yata, Nopadon Pirarat
Summary: The study focused on developing a mucoadhesive nano-immersion vaccine against columnaris disease in tilapia, using field isolates of Flavobacterium columnare obtained from clinical outbreaks in red tilapia. Results showed that the nano-vaccine induced greater antigen uptake and immune response in fish than conventional whole cell killed vaccines.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Veterinary Sciences
Anita M. Kelly, Nilima Renukdas, Louis Matthew Barnett, Benjamin H. Beck, Hisham A. Abdelrahman, Luke A. Roy
Summary: The aquaculture industry faces financial losses due to a bacterial disease called columnaris. Antibiotic treatment is expensive and leads to antibiotic resistance, so an alternative substance is needed. Kaolin clay has been shown to bind to columnaris bacteria, and this study confirmed its safety and efficacy in treating freshwater fish species in Arkansas, USA.
VETERINARY SCIENCES
(2023)
Article
Fisheries
Bingchao Wang, Fangzheng Zhu, Zechao Shi, Zhenyu Huang, Ruhan Sun, Qingchao Wang, Ouyang Gang, Wei Ji
Summary: In this study, the mhc IIA and mhc IIB genes of yellow catfish Pelteobagrus fulvidraco were cloned and characterized. Similar gene structure and expression patterns were observed for both mhc IIA and mhc IIB, and they play an important role in the anti-infection process.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Eric Peatman, Haitham Mohammed, Augustus Kirby, Craig A. Shoemaker, Mediha Yildirim-Aksoy, Benjamin H. Beck
Article
Fisheries
Honggang Zhao, Chao Li, John S. Hargrove, Bryant R. Bowen, Wilawan Thongda, Dongdong Zhang, Haitham Mohammed, Benjamin H. Beck, James D. Austin, Eric Peatman
Article
Fisheries
Dongdong Zhang, Benjamin H. Beck, Haitham Mohammed, Honggang Zhao, Wilawan Thongda, Zhi Ye, Qifan Zeng, Craig A. Shoemaker, S. Adam Fuller, Eric Peatman
FISH & SHELLFISH IMMUNOLOGY
(2018)
Article
Fisheries
Mediha Yildirim-Aksoy, Haitham Mohammed, E. Peatman, S. Adam Fuller, Benjamin H. Beck
NORTH AMERICAN JOURNAL OF AQUACULTURE
(2018)
Article
Agriculture, Dairy & Animal Science
H. Zhao, A. Fuller, W. Thongda, H. Mohammed, J. Abernathy, B. Beck, E. Peatman
Article
Parasitology
Justin M. Stilwell, Alvin C. Camus, John H. Leary, Haitham H. Mohammed, Matt J. Griffin
PARASITOLOGY RESEARCH
(2019)
Article
Veterinary Sciences
Stacey LaFrentz, Eva Abarca, Haitham H. Mohammed, Rosemary Cuming, Covadonga R. Arias
VETERINARY OPHTHALMOLOGY
(2020)
Article
Fisheries
Justin M. Stilwell, Matt J. Griffin, Thomas G. Rosser, Haitham H. Mohammed, Inga F. Sidor, Alvin C. Camus
JOURNAL OF FISH DISEASES
(2020)
Article
Biotechnology & Applied Microbiology
Paul Barbier, Tatiana Rochat, Haitham H. Mohammed, Gregory D. Wiens, Jean-Francois Bernardet, David Halpern, Eric Duchaud, Mark J. McBride
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Nicole C. Thunes, Rachel A. Conrad, Haitham H. Mohammed, Yongtao Zhu, Paul Barbier, Jason P. Evenhuis, David Perez-Pascual, Jean-Marc Ghigo, Ryan S. Lipscomb, John R. Schneider, Nan Li, Devon H. Erbes, Clayton Birkett, Benjamin R. LaFrentz, Timothy J. Welch, Mark J. McBride
Summary: Flavobacterium columnare, a pathogen that causes columnaris disease in freshwater fish, poses a major threat to sustainable aquaculture. Researchers have identified a genetically manipulable strain, MS-FC-4, which has facilitated the identification of virulence factors. Deletion of core genes eliminates virulence, and several secreted proteins have been identified as important for pathogenicity.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Fisheries
Dongdong Zhang, Haitham Mohammed, Zhi Ye, Melanie A. Rhodes, Wilawan Thongda, Honggang Zhao, Lauren N. Jescovitch, S. Adam Fuller, D. Allen Davis, Eric Peatman
Summary: This study simulated the natural infection of Amyloodinium ocellatum in cultured pompano and compared the gene expression in the gills of infected and control fish to explore the molecular mechanisms of infection. The results revealed differentially expressed genes associated with immune response, inflammation, reactive oxygen species, tissue repair, apoptosis, respiratory gas sensing, and lipid metabolism. This information can be utilized for disease surveillance, selection for host resistance, and development of therapeutic measures.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Khalid Shahin, Kaveramma Mukkatira, Zeinab Yazdi, Christine Richey, Kevin Kwak, Taylor Heckman, Haitham H. Mohammed, Cesar Ortega, Ruben Avendano-Herrera, Bill Keleher, Michael W. Hyatt, John D. Drennan, Mark Adkison, Matt J. Griffin, Esteban Soto
Summary: The study focuses on the development and validation of a TaqMan qPCR assay for the accurate and early detection of piscine lactococcosis. The developed assay showed high specificity, sensitivity, and accuracy in detecting the causative agents, as well as screening fish and environmental samples. This qPCR assay provides a reliable tool for diagnosing and managing piscine lactococcosis in fish farms and aquariums.
JOURNAL OF FISH DISEASES
(2022)
Article
Agriculture, Dairy & Animal Science
Jose F. Fernandez-Garayzabal, Stacey LaFrentz, Almudena Casamayor, Eva Abarca, Haitham H. Mohammed, Rosemary S. Cuming, Cova R. Arias, Lucas Dominguez, Ana Vela
Summary: This study identified and characterized a new commensal species, Corynebacterium conjunctivae, recovered from the conjunctival sac of healthy adult horses. The bacterial isolates displayed specific cellular morphology, biochemical properties, and gene sequences. This research provides important insights into the diversity of commensal bacteria in horse eyes.
Article
Fisheries
Salwa Mansour, Karima A. Bakry, Eman A. Alwaleed, Hassan Ahmed, Zeinab Al-Amgad, Haitham H. Mohammed, Walaa F. A. Emeish
Summary: The effects of dietary supplementation of nanocurcumin and two doses of florfenicol on African catfish were investigated. The results showed that adding nanocurcumin increased serum total protein levels and protected against liver and kidney damage caused by florfenicol. In addition, the inclusion of nanocurcumin in the diet improved the resistance to bacterial infection.
Article
Fisheries
Haitham H. Mohammed, Taylor L. Brown, Benjamin H. Beck, Mediha Yildirim-Aksoy, Rashida M. Eljack, Eric Peatman
JOURNAL OF APPLIED AQUACULTURE
(2019)