Article
Oncology
Karen Kai-Lin Fang, Jongbok Lee, Ismat Khatri, Yoosu Na, Li Zhang
Summary: The use of allogeneic CAR4-DNTs as adoptive cell therapy for T-cell malignancies is effective. CAR4-DNTs can effectively target T-ALL and PTCL and have superior cytotoxicity compared to empty-vector transduced DNTs.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Immunology
Lucia Lapazio, Monika Braun, Kaj Grandien
Summary: The activation of both CD8 and CD4 T cells is essential for a strong and long-lasting T cell immune response. The study shows that using antigen sequences fused to sequences derived from the H2-M and H2-O proteins can enhance CD4 T-cell activation, potentially improving the efficiency of mRNA- or DNA-based vaccines.
Article
Virology
Kai Qin, Sushma Boppana, Jonathan M. Carlson, Andrew Fiore-Gartland, Jacob Files, Jie Zeng, Jeffrey Edberg, Robbie B. Mailliard, Christina Ochsenbauer, Anju Bansal, Paul Goepfert
Summary: Adapted HIV epitopes can enhance viral trans-infection by promoting dendritic cell maturation. Vaccine recipients with high HLA-I-associated adaptation have a higher risk of HIV infection. These findings suggest the need to optimize HIV vaccine design considering HLA-I-associated adaptations to reduce infection risk.
JOURNAL OF VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Ken Miyaguchi, Hongqiang Wang, Keith L. Black, Stephen L. Shiao, Rongfu Wang, John S. Yu
Summary: Efficient activation of T cells with tumor-associated antigens (TAAs) to glioblastoma (GBM) stem cells was achieved through the use of three different antigen sources loaded onto dendritic cells (DCs). An activated T cell (ATC) protocol was developed and optimized for a phase I clinical trial to target GSCs. The synthetic peptide pool loading method showed significantly increased cytotoxicity towards target cells.
SCIENTIFIC REPORTS
(2023)
Article
Biology
Ellen C. N. van Vroonhoven, Lucas W. Picavet, Rianne C. Scholman, Noortje A. M. van den Dungen, Michal Mokry, Anouk Evers, Robert J. Lebbink, Jorg J. A. Calis, Sebastiaan J. Vastert, Jorg van Loosdregt
Summary: The expression of immune regulatory proteins is tightly regulated at various cellular processes, including at the RNA transcript level. It was found that m(6)A regulates the activation of CD4(+) T lymphocytes by regulating the expression of the co-stimulatory molecule CD40 ligand. This discovery contributes to our understanding of CD4(+) T cell activation, gene expression regulation, and autoimmune disease pathogenesis.
Article
Oncology
Camilla Bove, Silvia Arcangeli, Laura Falcone, Barbara Camisa, Rita El Khoury, Beatrice Greco, Anna De Lucia, Alice Bergamini, Attilio Bondanza, Fabio Ciceri, Chiara Bonini, Monica Casucci
Summary: This study investigated the role of CD4 and CD8 T cells in CD19 CAR-T cell responses and the development of cytokine release syndrome (CRS). CD4 CAR-T cells exhibited superior proliferation and activation potential compared to CD8 CAR-T cells. CD4 CAR-T cells were found to play a key role in the development of CRS, and CD4 CAR-T cells with embedded 4-1BB were associated with a safer profile. CD4 cells were crucial for maintaining long-term responses and the combination of CD4.BBz with CD8.28z CAR-T cells resulted in the lowest toxicity without compromising antitumor efficacy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Immunology
Juan Navarro-Barriuso, Maria Jose Mansilla, Bibiana Quirant-Sanchez, Aina Teniente-Serra, Cristina Ramo-Tello, Eva M. Martinez-Caceres
Summary: The use of vitamin D3-generated tolDC (vitD3-tolDC) has shown promising results in inducing antigen-specific hyporesponsiveness in T cells, reducing T(H)1 immune profiles and IFN-γ production. Transcriptomic analysis revealed a significant down-regulation of genes related to cell cycle and pro-inflammatory immune responses, with JUNB gene identified as a potential biomarker in this process. These findings highlight the regulatory properties and therapeutic potential of vitD3-tolDC in autoimmune diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Kazuhiro Mochizuki, Shogo Kobayashi, Nobuhisa Takahashi, Kotaro Sugimoto, Hideki Sano, Yoshihiro Ohara, Shin Mineishi, Yi Zhang, Atsushi Kikuta
Summary: In this study, a novel strategy was established to destroy pre-established tumors and induce potent antitumor immunity in mice using alloantigen-activated CD4(+) T cells (AAA-CD4(+)). Upon intratumoral injection, AAA-CD4(+) T cells demonstrated a significant expansion in the tumor, secretion of high levels of IFN-gamma and IL-2, and increased infiltration of host immune cells. The locally induced antitumor immunity elicited systemic protection, persisted over 6 months, and protected the animals from tumor re-challenge.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Feng Tao, Qingsong Ye, Yimiao Chen, Lifeng Luo, Haiheng Xu, Jialong Xu, Zhuo Feng, Chao Wang, Tao Li, Yuxuan Wen, Yiqiao Hu, Hong Dong, Xiaozhi Zhao, Jinhui Wu
Summary: Loading antigens onto the surface of flagellate bacteria modified by a cationic polymer can overcome the skin barrier and actively deliver antigens in the skin, promoting the activation of dendritic cells and enhancing immune responses. This approach shows potential in cancer therapy and COVID-19 immunization.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Microbiology
Xuejie Liu, Tianyi Zhao, Liangliang Wang, Minchao Li, Caijun Sun, Yuelong Shu
Summary: This study optimized the HA sequences of seasonal influenza viruses and designed a mosaic vaccine to enhance T-cell response. The results showed the potential of the recombinant mosaic antigen as an effective immunogen and provided a strategy for improving human protection against seasonal influenza virus.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Oncology
Tamara Hofer, Matteo Rossi, Susanna Carboni, Wilma Di Berardino Besson, Dorothee von Laer, Guido Wollmann, Madiha Derouazi, Marie-Laure Santiago-Raber
Summary: Developing new therapeutic cancer vaccines is crucial to combat tumor escape after conventional therapies in certain types of cancer. The study's multi-epitope vaccine can induce potent anti-tumor immune responses in various tumor models, laying a foundation for cancer immunotherapy.
Article
Biochemistry & Molecular Biology
Mariana P. Pinho, Guilherme A. Lepski, Roberta Rehder, Nadia E. Chauca-Torres, Gabriela C. M. Evangelista, Sarah F. Teixeira, Elizabeth A. Flatow, Jaqueline V. de Oliveira, Carla S. Fogolin, Nataly Peres, Analia Arevalo, Venancio Alves, Jose A. M. Barbuto, Patricia C. Bergami-Santos
Summary: This case study investigated the treatment of glioblastoma in a 25-year-old female patient with a dendritic-tumor cell fusion vaccine. The findings suggest that tumor-specific CD4+ T-cell numbers and phenotype could serve as biomarkers for treatment efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Multidisciplinary
Brianna L. Scotland, Andrea L. Cottingham, Jackline Joy M. Lasola, Stephen W. Hoag, Ryan M. Pearson
Summary: In this study, bioconjugate nanoparticles composed of poly(lactic-co-glycolic acid) and ovalbumin were developed to investigate the modulation of immune cell responses. The results showed that the antigen loading and formulation parameters of the nanoparticles played a crucial role in determining the T cell activation and differentiation. This work provides important insights for the design of nanoparticle-based immunotherapies.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Immunology
Hugues Allard-Chamard, Faisal Alsufyani, Naoki Kaneko, Kelly Xing, Cory Perugino, Vinay S. Mahajan, Joseph L. Wheat, George S. Deepe, James Loyd, Shiv Pillai
Summary: This study found that fibrotic diseases such as fibrosing mediastinitis may be induced and activated by fungal antigens, indicating that T cells, specifically CD4(+)CTLs, play a significant role in inflammatory fibrosis. The research provides important insights into the pathogenesis of idiopathic fibrotic diseases.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lucille F. van Beek, Pascal L. W. Welzen, Lisa U. Teufel, Irma Joosten, Dimitri A. Diavatopoulos, Jan van Hest, Marien de Jonge
Summary: The study demonstrated that displaying Fc fragments on the surface of polymersomes significantly increased interactions with granulocytes, monocytes, and NK cells, while CpG-decorated polymersomes were found to also interact with T and/or B cells. Stimulation of whole blood with Fc fragments and CpG-decorated polymersomes induced the production of interleukin, while naked polymersomes did not induce cytokine production. This suggests that bimodal targeting of different immune receptors on polymersomes can control specific immune induction for targeted vaccine delivery.
Letter
Ophthalmology
Jordan L. Mitchell, Laura Ganis, Benjamin T. Blacklock, Harry Petrushkin, Jayne C. Hope, Danielle A. Gunn-Moore
Summary: Tuberculosis is an ancient infectious disease that causes millions of deaths each year in humans, as well as significant morbidity and mortality in animals. Ocular tuberculosis is a recognized condition in humans, but its occurrence in animals, such as cattle and domestic cats, requires further investigation in order to improve treatment outcomes.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2022)
Article
Veterinary Sciences
J. L. Mitchell, J. Del Pozo, C. S. C. Woolley, R. Dheendsa, J. C. Hope, D. A. Gunn-Moore
Summary: The study aimed to differentiate between skin and lymph node lesions associated with M. bovis and M. microti using histological and immunohistochemical criteria. Results showed that feline M. bovis-associated lesions often featured large granulomas with central necrosis, while M. microti-associated lesions were characterized by small granulomas without necrosis. Interestingly, there were no significant differences in immunohistochemical marker expression between the two groups.
JOURNAL OF SMALL ANIMAL PRACTICE
(2022)
Article
Immunology
Heloisa M. Rutigliano, Aaron J. Thomas, Janae J. Umbaugh, Amanda Wilhelm, Benjamin R. Sessions, Rakesh Kaundal, Naveen Duhan, Brady A. Hicks, Donald H. Schlafer, Kenneth L. White, Christopher J. Davies
Summary: A significant rate of spontaneous abortion is observed in cattle pregnancies produced by somatic cell nuclear transfer (SCNT). Major histocompatibility complex class I (MHC-I) proteins are abnormally expressed on the surface of trophoblast cells from SCNT conceptuses. MHC-I compatibility between the dam and fetus prevents an exacerbated maternal immune response.
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2022)
Article
Biology
Stephen Meek, Tom Watson, Lel Eory, Gus McFarlane, Felicity J. Wynne, Stephen McCleary, Laura E. M. Dunn, Emily M. Charlton, Chloe Craig, Barbara Shih, Tim Regan, Ryan Taylor, Linda Sutherland, Anton Gossner, Cosmin Chintoan-Uta, Sarah Fletcher, Philippa M. Beard, Musa A. Hassan, Finn Grey, Jayne C. Hope, Mark P. Stevens, Monika Nowak-Imialek, Heiner Niemann, Pablo J. Ross, Christine Tait-Burkard, Sarah M. Brown, Lucas Lefevre, Gerard Thomson, Barry W. McColl, Alistair B. Lawrence, Alan L. Archibald, Falko Steinbach, Helen R. Crooke, Xuefei Gao, Pentao Liu, Tom Burdon
Summary: This study investigates the potential of porcine pluripotent stem cells to provide a limitless source of macrophages, demonstrating their molecular and functional characteristics, as well as their susceptibility to infection by pig pathogens. The study also shows the successful application of CRISPR/Cas9 gene editing in these cells, presenting new opportunities for researching host-pathogen interactions in pigs.
Article
Immunology
Sara Marzo, Mark Gray, Waywen Loh, Lindsey A. Waddell, C. Irene McGuinnes, Jayne C. Hope, Heather A. Mathie
Summary: The bovine afferent lymphatic cannulation model allows the study of lymphatic cells trafficking from the periphery directly ex-vivo. The time post-surgery did not significantly affect the volume of lymph or cell density, but a decrease in the percentage of gamma delta T-cells in afferent lymph was observed at 1 day post-cannulation. The activation state of dendritic cells in the naive host may be influenced by the cannulation procedure and duration of the experiment.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2022)
Article
Pathology
Jordan L. Mitchell, Laura MacDougall, Melanie J. Dobromylskyj, Ken Smith, Renata Stavinohova, Danielle A. Gunn-Moore, Jayne C. Hope, Emma Scurrell
Summary: This study aimed to explore the distribution and histopathological features of feline ocular mycobacterial lesions and characterize the immune cell population. The results showed that the choroid is the primary site of lesion development in most cases, and the lesions are typically granulomatous to pyogranulomatous.
VETERINARY PATHOLOGY
(2022)
Article
Microbiology
Hamza Khalid, Anouk van Hooij, Timothy K. Connelley, Annemieke Geluk, Jayne C. Hope
Summary: This study investigated the potential biomarkers for diagnosing bovine tuberculosis and found that IL-2, IP-10, and IFN gamma could be useful in discriminating between uninfected, BCG-vaccinated, and M. bovis-infected animals.
Article
Veterinary Sciences
Rosemary Blake, Kirsty Jensen, Neil Mabbott, Jayne Hope, Joanne Stevens
Summary: Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne's Disease, a prevalent chronic enteritis in ruminants worldwide. Understanding the initial interaction between MAP and the host is critical for the development of better diagnostic tools and new vaccines. In this study, three different multicellular in vitro models derived from bovine intestinal tissue were characterized for studying the interactions between MAP and host cells. The results showed that these models can be infected with MAP and may be useful in investigating the early stages of MAP pathogenesis.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Genetics & Heredity
Christopher J. Davies, Zhiqiang Fan, Kira P. Morgado, Ying Liu, Misha Regouski, Qinggang Meng, Aaron J. Thomas, Sang-Im Yun, Byung-Hak Song, Jordan C. Frank, Iuri V. Perisse, Arnaud Van Wettere, Young-Min Lee, Irina A. Polejaeva
Summary: The study generated sheep models with IFNAR deficiency by disrupting IFNAR genes using CRISPR/Cas9. These sheep have compromised immune responses to viral infections but are able to compensate using alternative interferon pathways. The model provides a unique opportunity to study the pathogenesis of viral infections and pregnancy recognition.
FRONTIERS IN GENETICS
(2022)
Article
Immunology
Alexandria E. Gillespie, Kathleen Loonie, Lucas Lefevre, Jayne C. Hope, Cynthia L. Baldwin, Timothy K. Connelley
Summary: This study investigates the TCR repertoires in cattle and finds that the TCR gamma repertoire is clonally stratified and essentially public, while the TCR delta repertoire shows higher levels of clonal diversity and is essentially private. Furthermore, the study suggests that TCR functions mainly as an innate-like receptor and does not act as a primary determinant of antigen specificity in the response to Leptospira.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Jordan L. Mitchell, Chris Wilson, Janet E. Alexander, Shelley G. Rhodes, Danielle A. Gunn-Moore, Jayne C. Hope
Summary: In order to facilitate the diagnosis of feline tuberculosis (TB), an optimized enzyme-linked immunosorbent assay (ELISA) was developed and tested. The ELISA showed a sensitivity of 64.3% and specificity of 100% when used on cats with culture-confirmed TB. When tested on cats with strongly suspected mycobacteriosis, the sensitivity was 32.9%. These results suggest that antibody-based diagnostics can be a useful adjunctive test for cases of TB missed by the IGRA.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2023)
Article
Immunology
Petra C. Fay, Najith Wijesiriwardana, Henry Munyanduki, Beatriz Sanz-Bernardo, Isabel Lewis, Ismar R. Haga, Katy Moffat, Arnoud H. M. van Vliet, Jayne Hope, Simon P. Graham, Philippa M. Beard
Summary: This study provides an in-depth analysis of the immune responses of calves experimentally inoculated with LSDV and highlights the differences in immune response between clinical and nonclinical cattle. It suggests that arthropod transmission leads to more widespread clinical disease and that early IgM production is a correlate of protection in LSD.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Christopher G. A. Mcgregor, Guerard W. Byrne, Zhiqiang Fan, Christopher J. Davies, Irina A. Polejaeva
Summary: This study developed genetically engineered sheep that can produce anti-Gal antibodies, which mirror the immunologic incompatibility currently seen in clinical heart valve implantation. The genetically engineered sheep provide a new advanced standard for preclinical testing of heart valves, taking into account the human immune responses to residual Gal antigen.
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2023)
Meeting Abstract
Immunology
H. Khalid, A. van Hooij, T. K. Connelley, A. Geluk, J. C. Hope
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Veterinary Sciences
Kate M. Sutton, Brigid Orr, Jayne Hope, Stina R. Jensen, Lonneke Vervelde
Summary: The study presents a method to generate 2D enteroids from 3D enteroids, providing a useful model for studying bovine epithelial cell biology with easy access to the apical surface of cells. It has the potential to investigate host-pathogen interactions and screen bioactive compounds.
VETERINARY RESEARCH
(2022)